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Elucidation of molecular basis of insect resistance in mungbean using comparative genomics information

Research Project

Project/Area Number 21380010
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Breeding science
Research InstitutionNational Institute of Agrobiological Sciences (2010-2011)
National Agricultural Research Organization (2009)

Principal Investigator

ISHIMOTO Masao  独立行政法人農業生物資源研究所, ダイズゲノム育種研究ユニット, ユニット長 (20355134)

Co-Investigator(Renkei-kenkyūsha) SUGAWARA Fumio  東京理科大学, 理工学部, 教授 (30192123)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywordsゲノム / 抵抗性 / 貯蔵害虫 / アズキゾウムシ / ホソヘリカメムシ / BACクローン / 環状ペプチド / RNA-Seq / 育種学 / 遺伝子 / 植物
Research Abstract

In order to elucidate the molecular basis of bruchid resistance derived from a wild accession of mungbean(Vigna radiata), we constructed a fine linkage map and a detailed physical map around the insect resistance locus(Br) using a large-scale segregating population and three mungbean BAC libraries. Recombinant lines and their progenies indicated that the two distinct regions in the same linkage group are responsible for the resistance. In addition, RNA-Seq analysis revealed one gene, which strongly expressed in developing seeds of the resistant mungbean lines, was identified from each region harboring the resistance gene(s). These two genes shared a high degree of homology, but the function of the genes as well as their homologous genes in other plant species has not been annotated yet.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (4 results)

All 2012 2011

All Presentation (4 results)

  • [Presentation] リョクトウの貯蔵害虫抵抗性に必要なゲノム領域の解析2012

    • Author(s)
      石本政男,寺石政義, Liu S,佐山貴司,廣瀬亜矢,中本有美,横田侑子,佐藤修正,平川英樹,加賀秋人
    • Organizer
      日本育種学会第121回講演会
    • Place of Presentation
      宇都宮市(宇都宮大学)
    • Year and Date
      2012-03-30
    • Related Report
      2011 Final Research Report
  • [Presentation] RNA-Seqによるリョクトウ貯蔵害虫抵抗性関連遺伝子の解析2012

    • Author(s)
      石本政男, 高木恭子, 佐山貴司, 横田侑子, Liu S, 廣瀬亜矢, 中本有美, 寺石政義, 加賀秋人
    • Organizer
      日本育種学会第121回講演会
    • Place of Presentation
      宇都宮大学
    • Year and Date
      2012-03-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] RNA-Seqによるリョクトウ貯蔵害虫抵抗性関連遺伝子の解析2011

    • Author(s)
      石本政男,高木恭子,佐山貴司,横田侑子, Liu S,廣瀬亜矢,中本有美,寺石政義,加賀秋人
    • Organizer
      日本育種学会第120回講演会
    • Place of Presentation
      福井市(福井県立大学)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Final Research Report
  • [Presentation] リョクトウの貯蔵害虫抵抗性に必要なゲノム領域の解析2011

    • Author(s)
      石本政男, 寺石政義, Liu S, 佐山貴司, 廣瀬亜矢, 中本有美, 横田侑子, 佐藤修正, 平川英樹, 加賀秋人
    • Organizer
      日本育種学会第120回講演会
    • Place of Presentation
      福井県立大学
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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