Analysis of molecular basis for enhanced symbiotic nitrogen fixation and its application for leguminous crops production.

• Project/Area Number: 21380016
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Crop science/Weed science
• Research Institution: Saga University
• Principal Investigator: SUZUKI Akihiro 佐賀大学, 農学部, 准教授 (50305108)
• Co-Investigator(Kenkyū-buntansha): ARIMA Susumu 佐賀大学, 農学部, 教授 (90140954)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥16,380,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥3,780,000); Fiscal Year 2012: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: ミヤコグサ / ダイズ / 窒素固定 / 共生 / 根粒菌 / 遺伝子 / 根粒 / Enf1遺伝子 / ABA / 表現型 / 変異体 / スクリーニング

Research Abstract

Root nodule formation and nitrogen fixation activity was enhanced in enf1 mutant of model leguminous plant Lotus japonicus. In this project, we showed that the number of root nodules was increased by the down-regulation of TRX gene expression. This result indicates that TRX gene is one of a gene that function as an enhancer of symbiotic nitrogen fixation. Soybean mutant line NO. 57 that were isolated by the ABA screening showed enhanced nitrogen fixation activity and also showed higher seed yields.

Research Products

• [Journal Article] Quantitative trait locus analysis of symbiotic nitrogen fixation activity in the model legume Lotus japonicus (2012)
  • Author(s): Tominaga A, Gondo T, Akashi R, Zheng SH, Arima S, Suzuki A
  • Journal Title: Journal of Plant Research Volume: 125 Issue: 3 Pages: 395-406
  • DOI: 10.1007/s10265-011-0459-1
  • Peer Reviewed
• [Journal Article] Additional cause for reduced JA-Ile in the root of a Lotus japonicus phyB mutant (2012)
  • Author(s): Shigeyama T, Tominaga A, Arima S, Sakai T, Inada , Jikumaru , Kamiya , Uchiumi , Abe M, Hashiguchi M, Akashi R, Hirsch AM, and Suzuki A, Shigeyama T, Tominaga A, Arima S, Sakai T, Inada , Jikumaru , Kamiya , Uchiumi , Abe M, Hashiguchi M, Akashi R, Hirsch AM, and Suzuki A
  • Journal Title: Plant Signal Behav. Volume: 7 Issue: 7 Pages: 1-3
  • DOI: 10.4161/psb.20407
  • Peer Reviewed
• [Journal Article] The National BioResource Project (NBRP) Lotus and Glycine in Japan (2012)
  • Author(s): Hashiguchi M, Abe J, Aoki T, Anai T, Suzuki A, Akashi R
  • Journal Title: Breeding Science Volume: 61 Issue: 5 Pages: 453-461
  • DOI: 10.1270/jsbbs.61.453
  • NAID: 130004057126
  • ISSN: 1344-7610, 1347-3735
  • Peer Reviewed
• [Journal Article] Quantitative trait locus analysis of symbiotic nitrogen fixation activity in the model legume Lotus Japonicus (2012)
  • Author(s): Tominaga A, Gondo T, Akashi R, Zheng SH, Arima S, Suzuki A
  • Journal Title: Journal of Plant Research Volume: 126 Pages: 395-406
  • NAID: 10030652669
  • Peer Reviewed
• [Journal Article] Lotus japonicus nodulation is photomorphogenetically controlled by sensing the red/far red (R/FR) ratio through jasmonic acid (JA) signaling (2011)
  • Author(s): Suzuki A, Suriyagoda L, Shigeyama T, Tominaga A, Sasaki M, Hiratsuka Y, Yoshinaga A, Arima S, Agarie S, Sakai T, Inada S, Jikumaru Y, Kamiya Y, Uchiumi T, Abe M, Hashiguchi M, Akashi R, Sato S, Kaneko T, Tabata S, Hirsch AM
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 108 Issue: 40 Pages: 16837-16842
  • DOI: 10.1073/pnas.1105892108
  • Peer Reviewed
• [Journal Article] Effect of abscisic acid on symbiotic nitrogen fixation activity in the root nodules of Lotus japonicus. (2010)
  • Author(s): A.Tominaga, M.Nagata, K.Futsuki, H.Abe, T.Uchiumi, M.Abe, K.Kucho, M.Hashiguchi, R.Akashi, A.Hirsch, S.Arima, A.Suzuki
  • Journal Title: Plant Signaling & Behavior Volume: 5 Pages: 440-443
• [Journal Article] Enhanced nodulation and nitrogen fixation in the ABA low-sensitive mutant enf1 (enhanced nitrogen fixation 1) of Lotus japonicus (2009)
  • Author(s): A. Tominaga, M. Nagata, K. Futsuki, H. Abe, T. Uchiumi, M. Abe, K. Kucho, M. Hashiguchi, R. Akashi, A. Hirsch, S. Arima and A. Suzuki
  • Journal Title: Plant Physiology Volume: 151 Pages: 1965-1976
  • URL: http://dx.doi.org/10.1104/pp.109.142638
  • Peer Reviewed
• [Journal Article] Enhanced nodulation and nitrogen fixation in the ABA low-sensitive mutant enf1(enhanced nitrogen fixation 1)of Lotus japonicus. (2009)
  • Author(s): A.Tominaga, M.Nagata, K.Futsuki, H.Abe, T.Uchiumi, M.Abe, K.Kucho, M.Hashiguchi, R.Akashi, A.Hirsch, S.Arima, A.Suzuki
  • Journal Title: Plant Physiology 151 Pages: 1965-1976
  • Peer Reviewed
• [Presentation] 高等植物への菌根菌感染は光の質と量によって制御される (2013)
  • Author(s): 鈴木章弘等
  • Organizer: 日本作物学会第235回講演会
  • Place of Presentation: 川崎市
• [Presentation] Molecular mechanism of improved symbiotic phenotype in ABA low-sensitive mutant enf1 of Lotus japonicus (2012)
  • Author(s): A. Tominaga, S. Arima, A. Suzuki et al
  • Organizer: 第2回アジア窒素固定会議
  • Place of Presentation: プーケット(タイ)
  • Year and Date: 2012-10-28
• [Presentation] Expression of b-1,3-glucanasegene in autoregulation of nodulation (2012)
  • Author(s): K. Osuki, A. Suzuki et al
  • Organizer: 第2回アジア窒素固定会議
  • Place of Presentation: プーケット(タイ)
  • Year and Date: 2012-10-28
• [Presentation] A possible role of vitamin B6 metabolic pathway of Mesorhizobium loti in symbiosis with Lotus japonicus. (2012)
  • Author(s): A. Suzuki et al.
  • Organizer: 第１０回ヨーロッパ窒素固定会議
  • Place of Presentation: ミュンヘン（ドイツ）
• [Presentation] Expression of b-1,3-glucanase gene in autoregulation of nodulation (2012)
  • Author(s): K. Osuki et al.
  • Organizer: 第２回アジア窒素固定会議
  • Place of Presentation: プーケット（タイ）
• [Presentation] Characterization of LjGlu1, b-1,3-glucanase gene of Lotus japonicus. (2012)
  • Author(s): S. Hashimoto et al.
  • Organizer: 第２回アジア窒素固定会議
  • Place of Presentation: プーケット（タイ）
• [Presentation] Molecular mechanism of improved symbiotic phenotype in ABA low-sensitive mutant enf1 of Lotus japonicus. (2012)
  • Author(s): A. Tominaga et al.
  • Organizer: 第２回アジア窒素固定会議
  • Place of Presentation: プーケット（タイ）
• [Presentation] Low R/FR decreased mycorrhizal infection in higher plants. (2012)
  • Author(s): M. Nagata et al.
  • Organizer: 第２回アジア窒素固定会議
  • Place of Presentation: プーケット（タイ）
• [Presentation] ビタミンB_6分解というミヤコグサ・根粒菌共生の新たな意義 (2012)
  • Author(s): 富永晃好, 等
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学
• [Presentation] 根粒形成のための避陰反応 (2011)
  • Author(s): 鈴木章弘
  • Organizer: 第5回ダイズ研究会
  • Place of Presentation: 札幌市
  • Year and Date: 2011-03-06
• [Presentation] Expression of an abscisic acid-re sponsive b-1,3-glucanase in autoregulation of nodulation (2011)
  • Author(s): Ken-ichi Osuki, et al
  • Organizer: 17^<th> International congress on nitrogen fixation
  • Place of Presentation: Fremantle, Australia
• [Presentation] Lotus japonicus nodulates when it sees red (2011)
  • Author(s): Akihiro Suzuki, et al
  • Organizer: 17^<th> International congress on nitrogen fixation
  • Place of Presentation: Fremantle, Australia
• [Presentation] Quantitative trait locus analysis of symbiotic nitrogen fixation activity in the model legume Lotus japonicus (2011)
  • Author(s): Akiyoshi Tminaga, et al
  • Organizer: Model Legume Congress 2011
  • Place of Presentation: Sainte Maxime, France
• [Presentation] ミヤコグサにおける共生窒素固定活性に関するQTL解析 (2011)
  • Author(s): 富永晃好, 等
  • Organizer: 第21回植物微生物研究会
  • Place of Presentation: 岡山大学
• [Presentation] マメ科植物の共生窒素固定能増強のメカニズムとその応用 (2010)
  • Author(s): 鈴木章弘
  • Organizer: 第5回九州育種談話会
  • Place of Presentation: 佐賀市
  • Year and Date: 2010-11-19
• [Presentation] Introduction and utilization of Legume Base in Lotus and Glycine bioresources (2010)
  • Author(s): M.Hashiguchi, J.Abe, T.Aoki, T.Anai, A.Suzuki, R.Akashi
  • Organizer: 1st Asian Conference on Plant-Microbe Symbiosis and Nitrogen Fixation
  • Place of Presentation: 宮崎市
  • Year and Date: 2010-09-22
• [Presentation] マメ科植物の共生窒素固定能増強のメカニズムとその応用 (2010)
  • Author(s): 鈴木章弘
  • Organizer: 日本植物病理学会平成22年度植物感染生理談話会
  • Place of Presentation: 唐津市
  • Year and Date: 2010-08-18
• [Presentation] ミヤコグサのABA低感受性変異体enf1の共生窒素固定能と応用の可能性 (2010)
  • Author(s): 鈴木章弘
  • Organizer: 植物生理学会シンポジウム
  • Place of Presentation: 熊本大学
• [Book] 作物学用語事典 (2010)
  • Author(s): 鈴木章弘,他
  • Publisher: 農文協
• [Remarks]
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• [Remarks]
  • URL: http://extwww.cc.saga-u.ac.jp/~azuki/top.html
• [Remarks]
  • URL: http://extwww.cc.saga-u.ac.jp/~azuki/top.html
• [Remarks]
  • URL: http://extwww.cc.saga-u.ac.jp/~azuki/top.html