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Genomic research and their utilization for selection marker development in Japanese chestnut

Research Project

Project/Area Number 21380020
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Horticulture/Landscape architecture
Research InstitutionIbaraki University

Principal Investigator

INOUE Eiichi  茨城大学, 農学部, 准教授 (90292482)

Co-Investigator(Kenkyū-buntansha) ANZAI Hiroyuki  茨城大学, 遺伝子実験施設, 教授 (20323214)
Research Collaborator KASUMI Masakazu  茨城県農業総合センター, 生物工学研究所, 室長(協力時)
TAKATSU Yasumasa  茨城県農業総合センター, 生物工学研究所, 室長(協力時)
Project Period (FY) 2009-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywordsクリ / ゲノム / SSRマーカー / DNAマーカー / 連鎖地図 / QTL解析 / 種間雑種 / ニホングリ / QTL / SSR / 花成遺伝子 / 堅果形質 / マイクロサテライト / AP1 / AFLP
Research Abstract

In the fruit breeding, shortening the selection period is very important because long years, considerable costs, and labor are needed for the selection.
Nowadays, the breeding efficiency could be improved by using the marker-assisted selection (MAS) according to the genomic information. However we had no enough genomic information about chestnuts. In this research we constructed genomic basis for realizing MAS in Japanese chestnut.

Report

(6 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (35 results)

All 2014 2013 2012 2011 2010 2009 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (26 results) Remarks (3 results)

  • [Journal Article] 花粉遮断試験によるクリ品種間における自家結実性の評価とSSR マーカーを用いた自家結実実生の遺伝解析2012

    • Author(s)
      井上栄一・本間貴司・佐々木道康・郷内武・霞正一
    • Journal Title

      園芸学研究(園芸学会)

      Volume: 11 Pages: 199-203

    • NAID

      130004938741

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 花粉遮断試験によるクリ品種間における自家結実性の評価とSSRマーカーを用いた自家結実実生の遺伝解析2012

    • Author(s)
      井上栄一・本間貴司・佐々木道康・郷内武・霞正一
    • Journal Title

      園芸学研究

      Volume: 11(2) Pages: 199-203

    • NAID

      130004938741

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] クリ果実における糖とデンプン含有量の品種間差並びに,加工グリの不良品発生要因2011

    • Author(s)
      本間貴司・原弘道・阮樹安・陳喜忠・王志運・井上栄一・月橋
    • Journal Title

      全球視角下的当代農業問題研究(中国農業科学技術)

      Volume: 2 Pages: 41-54

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of SSR Markers in Chinese chestnuts and their characterization in diverse chestnuts cultivars2009

    • Author(s)
      Inoue, E., L. Ning, H. Hara, S. Ruan and H. Anzai
    • Journal Title

      Journal of the American Society for Horticultural Science

      Volume: 134 Pages: 1-8

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] ニホングリとモーパングリの種間雑種の特性2009

    • Author(s)
      霞正一・郷内武・井上栄一・眞部徹・佐久間文雄
    • Journal Title

      茨城県農業総合センター生物工学研究所研究報告

      Volume: 11 Pages: 33-44

    • NAID

      220000114737

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of SSR Markers in Chinese Chestnuts and their Characterization in Diverse Chestnuts Cultivars2009

    • Author(s)
      Eiichi Inoue, Lin Ning, Hiromichi Hara, Shuan Ruan, Hiroyuki Anzai
    • Journal Title

      J.AMER.SOC.HORT.SCI 134(6)

      Pages: 1-8

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] QTL Analysis for Nuts Qualities in Interspecific Chestnut Population2014

    • Author(s)
      Eiichi Inoue,Michiyasu Sasaki
    • Organizer
      The 29th International Horticultural Congress 2014
    • Place of Presentation
      ブリスベン,(オーストラリア)
    • Related Report
      2013 Final Research Report
  • [Presentation] Isolation and characterization of the flowering-related genes in chestnut2014

    • Author(s)
      Eiichi Inoue,Tsunoda Suzuna, Takashi Kodama, Yasumasa Takatsu and Hisao Higashio
    • Organizer
      ISHS Symposium "Physiological Principles and Their Application to Fruit Production"
    • Place of Presentation
      ジェニーバ(アメリカ)
    • Related Report
      2013 Final Research Report
  • [Presentation] Isolation and characterization of the flowering-related genes in chestnut2014

    • Author(s)
      Eiichi Inoue, Tsunoda Suzuna, Takashi Kodama, Yasumasa Takatsu and Hisao Higashio
    • Organizer
      ISHS Symposium “Physiological Principles and Their Application to Fruit Production”
    • Place of Presentation
      Geneva, NY, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] QTL Analysis for Nuts Qualities in Interspecific Chestnut Population2014

    • Author(s)
      Eiichi Inoue and Michiyasu Sasaki
    • Organizer
      The 29th International Horticultural Congress 2014
    • Place of Presentation
      Brisbane Queensland Australia
    • Related Report
      2013 Annual Research Report
  • [Presentation] ニホングリとモーパングリの種間雑種における堅果形質の変異2013

    • Author(s)
      岡部慎也,井上栄一, 他
    • Organizer
      日本育種学会第123回講演会
    • Place of Presentation
      東京農業大学
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Presentation] クリにおける開花関連遺伝子の単離と解析2013

    • Author(s)
      角田鈴奈,井上栄一, 他
    • Organizer
      園芸学会平成25年度春季大会
    • Place of Presentation
      東京農工大学
    • Related Report
      2013 Final Research Report 2012 Annual Research Report
  • [Presentation] クリにおけるAP1様遺伝子の単離と発現解析2012

    • Author(s)
      角田鈴奈,井上栄一, 他
    • Organizer
      園芸学会平成24年度春季大会
    • Place of Presentation
      大阪府立大学
    • Year and Date
      2012-03-29
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] クリ種間雑種におけるヨーロッパグリ由来SSRマーカーの遺伝2012

    • Author(s)
      岡部慎也,井上栄一, 他
    • Organizer
      平成24年度果樹バイテク研究会
    • Place of Presentation
      弘前商工会議所会館
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic mapping and QTL analysis in interspecific chestnut population2012

    • Author(s)
      Eiichi Inoue
    • Organizer
      2012 American Society for Horticultural Science
    • Place of Presentation
      マイアミ(アメリカ)
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic mapping and QTL analysis in interspecific chestnut population2012

    • Author(s)
      Eiichi Inoue
    • Organizer
      American Society for Horticultural Science
    • Place of Presentation
      Miami, Florida, USA
    • Related Report
      2012 Annual Research Report
  • [Presentation] クリ種間雑種におけるヨーロッパグリ由来SSRマーカーの遺伝2012

    • Author(s)
      岡部慎也
    • Organizer
      平成24年度 果樹バイテク研究会
    • Place of Presentation
      弘前商工会議所会館
    • Related Report
      2012 Annual Research Report
  • [Presentation] ニホングリ'石鎚'とモーパングリの種間雑種における諸形質の解析2011

    • Author(s)
      角田鈴奈,井上栄一
    • Organizer
      茨城県園芸談話会
    • Place of Presentation
      茨城県農業総合センター
    • Year and Date
      2011-11-21
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] ニホングリ'石鎚'とクリ野生種モーパングリの種間雑種における形態及び開花特性の変異2011

    • Author(s)
      角田鈴奈,井上栄一, 他
    • Organizer
      園芸学会平成23年度秋季大会
    • Place of Presentation
      岡山大学
    • Year and Date
      2011-09-25
    • Related Report
      2013 Final Research Report 2011 Annual Research Report
  • [Presentation] クリにおける遺伝学的連鎖地図の構築とQTL 解析2011

    • Author(s)
      井上栄一, 他
    • Organizer
      平成23年度果樹バイテク研究会
    • Place of Presentation
      沖縄県庁北部合同庁舎
    • Year and Date
      2011-07-11
    • Related Report
      2013 Final Research Report
  • [Presentation] クリにおける遺伝学的連鎖地図の構築とQTL解析2011

    • Author(s)
      井上栄一・佐々木道康
    • Organizer
      平成23年度 果樹バイテク研究会
    • Place of Presentation
      沖縄県庁北部合同庁舎
    • Year and Date
      2011-07-11
    • Related Report
      2011 Annual Research Report
  • [Presentation] ニホングリとモーパングリの種間雑種を用いた遺伝学的連鎖地図の構築2011

    • Author(s)
      佐々木道康,井上栄一, 他
    • Organizer
      園芸学会平成23年度春季大会
    • Place of Presentation
      宇都宮大学農学部
    • Year and Date
      2011-03-20
    • Related Report
      2013 Final Research Report 2010 Annual Research Report
  • [Presentation] Nuclear and Chloroplast Microsatellite Diversity in Chestnuts2010

    • Author(s)
      Eiichi Inoue, Miki Kurabayashi, Lin Ning, Takashi Homma, Hiromichi Hara
    • Organizer
      International Hotisultural Congless 2010
    • Place of Presentation
      ポルトガル共和国リスボン
    • Year and Date
      2010-08-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Nuclear and Chloroplast Microsatellite Diversity in Chestnuts2010

    • Author(s)
      Eiichi Inoue, 他
    • Organizer
      The 28th International Horticultural Congress 2010
    • Place of Presentation
      リスボン,(ポルトガル)
    • Year and Date
      2010-08-24
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic Comparison of Chestnuts and the Related Species in Fagaceae by cpSSR markers2010

    • Author(s)
      Eiichi Inoue
    • Organizer
      2010 American Society for Horticultural Science
    • Place of Presentation
      パームデザート(アメリカ)
    • Year and Date
      2010-08-05
    • Related Report
      2013 Final Research Report
  • [Presentation] Genetic Comparison of Chestnuts and the Related Species in Fagaceae by cpSSR markers2010

    • Author(s)
      Eiichi Inoue, Lin Ning, Shuan Ruan, Hiroyuki Anzai, Hiromichi Hara
    • Organizer
      The 2010 American Society for Horticultural Science Annual Conference
    • Place of Presentation
      米国パームデザート
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 茨城県特産果樹のゲノム研究基盤の整備について2010

    • Author(s)
      井上栄一, 他
    • Organizer
      シンポジウム 次世代シークエンサーとDNA 塩基配列情報の農業研究への展開
    • Place of Presentation
      茨城大学農学部
    • Year and Date
      2010-02-26
    • Related Report
      2013 Final Research Report
  • [Presentation] 茨城県特産果樹のゲノム研究基盤の整備について2010

    • Author(s)
      井上栄一, 郷内武, 霞正一
    • Organizer
      シンポジウム 次世代シークエンサーとDNA塩基配列情報の農業研究への展開
    • Place of Presentation
      茨城大学農学部
    • Year and Date
      2010-02-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] アジアのクリの遺伝学的分類(第10 報)クリ種間および種内における葉緑体SSR 遺伝子座の変異2009

    • Author(s)
      井上栄一,倉林樹, 他
    • Organizer
      園芸学会平成21年度秋季大会
    • Place of Presentation
      秋田大学
    • Year and Date
      2009-09-26
    • Related Report
      2013 Final Research Report
  • [Presentation] アジアのクリの遺伝学的分類(第10報)クリ種間および種内における葉緑体SSR遺伝子座の変異2009

    • Author(s)
      井上栄一, 倉林樹, 寧林, 本間貴司, 原弘道
    • Organizer
      園芸学会平成21年度秋季大会
    • Place of Presentation
      秋田大学
    • Year and Date
      2009-09-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] Interspecific Hybridization for Genetic Mapping in Chestnuts2009

    • Author(s)
      Eiichi Inoue, 他
    • Organizer
      ISHS Symposium "Molecular Markers in Horticulture"
    • Place of Presentation
      オレゴン(アメリカ)
    • Year and Date
      2009-07-29
    • Related Report
      2013 Final Research Report
  • [Presentation] Interspecific Hybridization for Genetic Mapping in Chestnuts2009

    • Author(s)
      Eiichi Inoue, Michiyasu Sasaki, Takashi Homma, Masakazu Kasumi
    • Organizer
      Molecular Markers in Horticulture Symposium
    • Place of Presentation
      米国オレゴン州立大学
    • Year and Date
      2009-07-29
    • Related Report
      2009 Annual Research Report
  • [Remarks] シンポジウム開催 『次世代シークエンサーとDNA 塩基配列情報の農業研究への展開-果樹ゲノム研究から品種識別まで-』,茨城大学農学部研究推進委員会,2010年2月26日,茨城大学農学部

    • Related Report
      2013 Final Research Report
  • [Remarks]

    • URL

      http://info.ibaraki.ac.jp/scripts/websearch/index.htm

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://info.ibaraki.ac.jp/scripts/websearch/index.htm

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2019-07-29  

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