| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2011: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Fiscal Year 2010: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2009: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
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| Research Abstract |
A host seven-pass transmembrane protein TOM1 and a small GTP-binding protein ARL8 are required for efficient multiplication of tomato mosaic virus(ToMV) RNA. When in vitro ToMV RNA replication reactions using evacuolated plant protoplast extracts were performed using Arabidopsis thaliana membranes that lack either one of these proteins, negative-strand RNA synthesis was hardly detectable. When the replication protein of ToMV was co-expressed with TOM1 and ARL8, the proteins gained guanylyltransferase activity, but could not catalize ToMV RNA replication. Thus, interaction with TOM1-and ARL8-containing membranes confers guanylyltransferase activity to ToMV replication protein, however, it is not sufficient for the activation of its RNA polymerase activity.
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