Defence systems of social insects toentomopathogenic microorganisms

• Project/Area Number: 21380037
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: Kyushu University
• Principal Investigator: SHIMIZU Susumu 九州大学, 大学院・農学研究院, 教授 (20187454)
• Co-Investigator(Kenkyū-buntansha): TAKAGI Masami 九州大学, 大学院・農学研究院, 教授 (20175425) ; AOKI Chisa 九州大学, 大学院・農学研究院, 准教授 (20264103) ; IIYAMA Kazuhiro 九州大学, 大学院・農学研究院, 助教 (70325489)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥19,110,000 (Direct Cost: ¥14,700,000、Indirect Cost: ¥4,410,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
• Keywords: 社会性昆虫 / 病原微生物 / 病気抵抗性 / 昆虫病理 / イエシロアリ / 触角 / 昆虫病原糸状菌 / 匂い / 分離法 / 系統分類 / 釣り餌法

Research Abstract

Termites, Coptotermes formosanusShiraki, reared individually, were highly susceptible to entomopathogenic fungi, while termites reared in groups were highly resistant. Our results suggested that self-grooming behavior isless effective, but mutual grooming is very effective in the removal of conidia from cuticles of their nestmates.Grooming behavior can at times lead to attack and cannibalism behaviors. The results of this study also indicated that contact with a pathogen induces behavioral changes in termite disease-prevention behaviors.

Research Products

• [Journal Article] Odor aversion and pathogen-removal efficiency in grooming behavior of the Termite Coptotermes formosanus (2012)
  • Author(s): Yanagawa, A., Fujiwara-Tsuji, N., Akino, T., Yoshimura, T., Yanagawa, T., and Shimizu, S
  • Journal Title: PLOS ONE Volume: Volume 7 | Issue 10
  • NAID: 120004920430
• [Journal Article] Odor aversion and pathogen-removal efficiency in grooming behavior of the Termite Coptotermes formosanus (2012)
  • Author(s): Yanagawa, A., Fujiwara-Tsuji, N., Akino, T., Yoshimura, T., Yanagawa, T., and Shimizu, S. October 2012 | Volume 7 | Issue 10 | e4741
  • Journal Title: PLOS ONE Volume: 7
  • NAID: 120004920430
  • Peer Reviewed
• [Journal Article] Musty odor of entomopathogens enhances disease-prevention behaviors in the termiteCoptotermes formosanus (2011)
  • Author(s): Yanagawa, A., Fujiwara-Tsuji, N., Akino, T., Yoshimura, T., Yanagawa, T., and Shimizu, S.
  • Journal Title: Journal of Invertebrate Pathology Volume: 108 Pages: 1-6
• [Journal Article] Behavioral changes in the termite, Coptotermes formosanus (Isoptera) (2011)
  • Author(s): Yanagawa A., Fujiwara-Tsujii, N., Akino, T., Yoshimura T., Yanagawa, T., Shimizu, S
  • Journal Title: inoculated with six fungal isolates Journal of Invertebrate Pathology Volume: 107 Pages: 100-106
• [Journal Article] Phylogenetic analysisof Metarhiziumspp (2011)
  • Author(s): Nishi, O., Hasegawa, K., Iiyama, K., Yasunaga-Aoki, C., and Shimizu, S
  • Journal Title: isolated from soil in Japan Applied Entomology and Zoology Volume: 46 Pages: 301-309
• [Journal Article] Phylogenetic analysis of Metarhizium spp. isolated from soil in Japan (2011)
  • Author(s): Oumi Nishi, Keiichi Hasegawa, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu
  • Journal Title: Applied Entomology and Zoology Volume: 36 Pages: 301-309
  • NAID: 40018958890
  • Peer Reviewed
• [Journal Article] Musty odor of entomop athogens enhances disease-prevention behaviors in the termite Coptotermes formosanus (2011)
  • Author(s): Aya Yanagawa, Nao Fujiwara-Tsujii, Toshiharu Akino, Tsuyoshi Yoshimura, Takashi Yanagawa, Susumu Shimizu
  • Journal Title: Journal of Insect Pathology Volume: 108 Pages: 1-6
  • NAID: 120003517941
  • Peer Reviewed
• [Journal Article] Influence of fungal ordor on grooming behavior of the termite (2010)
  • Author(s): Yanagawa, A., Yokohari, F., and Shimizu, S
  • Journal Title: Coptotermes formosanus Journal of Insect Science Volume: 10
• [Journal Article] Virulence of Metarhizium anisopliae var. majusisolated from soils against termite (2010)
  • Author(s): Nishi, O., Iiyama, K., Yasunaga-Aoki, C., and Shimizu, S
  • Journal Title: Coptotermes formosanus Shiraki Japanese Journal of Environmental Entomolology and Zoology Volume: 21 Pages: 29-35
• [Journal Article] Influence of fungal ordor on grooming behavior of the termite, Coptotermes formosanus (2010)
  • Author(s): Yanagawa, A., Yokohari, F., Shimizu, S
  • Journal Title: Journal of Insect Science Volume: Insect Science
  • Peer Reviewed
• [Journal Article] Virulence of Metarhizium anisopliae var. majus isolated from soils against termite, Coptotermes formosanus Shiraki (2010)
  • Author(s): Nishi, O., Iiyama, K., Yasunaga-Aoki, C., Shimizu, S.
  • Journal Title: Jpn.J.Environ.Entomol.Zool. Volume: 21 Pages: 29-35
  • NAID: 40017000046
  • Peer Reviewed
• [Journal Article] The role of antennae in removing entomopathogenic fungi from cuticle of the termite (2009)
  • Author(s): Yanagawa, A. Yokohari, F., and Shimizu, S
  • Journal Title: Coptotermes formosanus. Journal of Insect Science Volume: 9 Pages: 1-9
• [Journal Article] Classification and distribution of antennal sensilla of the termite Coptotermes formosanus (Isoptera : Rhinotermitidae) (2009)
  • Author(s): Yanagawa, A., Shimizu, S., Noma, K., Nishikawa, M., Ogino, K., Yokohari, F.
  • Journal Title: Sociobiology Volume: 54 Pages: 327-347
  • Peer Reviewed
• [Journal Article] The role of antennae in removing entomopathogenic fungi from cuticle of the termite, Coptotermes formosanus (2009)
  • Author(s): Yanagawa, A., Yokohari, F., Shimizu, S.
  • Journal Title: Journal of Insect Science Volume: 9 Pages: 1-9
  • Peer Reviewed
• [Presentation] Sensitivity of behavior to pathogen-related ordor in the termite (2012)
  • Author(s): Yanagawa, A., Fujiwara-Tsuji, N., Akino, T., Yoshimura, T., and Shimizu, S
  • Organizer: Coptotermes formosanus International Congress on Invertebrate Pathology and Microbial Control and 45th Annual Meeting of the Society for Invertebrate Pathology, P75, Centro de Convenciones de la UCA Puerto Madero
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2012-08-06
• [Presentation] Host-dependent lineage diversification of Scarabaeidae-specific pathogen Metarhizium majus (2012)
  • Author(s): Nishi O., Iiyama, K., Yasunaga-Aoki, C., and Shimizu, S
  • Organizer: International Congress on Invertebrate Pathology and Microbial Control and 45th Annual Meeting of the Society for Invertebrate Pathology, P60, Centro de Convenciones de la UCA Puerto Madero
  • Place of Presentation: Buenos Aires, Argentina
  • Year and Date: 2012-08-06
• [Presentation] 緑膿菌Pseudomonas aeruginosaのタンパク質分泌機構変異株の作出と病原力の評価 (2012)
  • Author(s): 大峠勝寛・飯山和弘・李在萬・日下部宜宏・青木智佐・清水進
  • Organizer: 平成24年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 九州大学箱崎キャンパス文系地区
  • Year and Date: 2012-03-19
• [Presentation] 大腸菌発現系を用いた組換えセラリシン様メタロプロテアーゼの発現および精製 (2012)
  • Author(s): 河野康亮・飯山和弘・李在萬・日下部宜宏・青木智佐・清水進
  • Organizer: 平成24年度蚕糸・昆虫機能利用学術講演会
  • Place of Presentation: 九州大学箱崎キャンパス文系地区
  • Year and Date: 2012-03-18
• [Presentation] Host-dependent lineage diversification of Scarabaeidae-specific pathogen Metarhizium majus (2012)
  • Author(s): Nishi O., Iiyama, K., Yasunaga-Aoki, C., and Shimizu, S
  • Organizer: International Congress on Invertebrate Pathology and Microbial Control
  • Place of Presentation: Buenos Aires, Argentina
• [Presentation] Sensitivity of behavior to pathogen-related ordor in the termite, Coptotermes formosanu (2012)
  • Author(s): Yanagawa, A., Fujiwara-Tsuji, N., Akino, T., Yoshimura, T., and Shimizu, S.
  • Organizer: International Congress on Invertebrate Pathology and Microbial Control
  • Place of Presentation: Buenos Aires, Argentina
• [Presentation] イモゾウムシ病原性Farinocystis様原虫の宿主範囲の調査 (2012)
  • Author(s): 新見はるか・鶴田幸成・青木智佐・松山隆志・飯山和弘・清水進
  • Organizer: 第56回日本応用動物昆虫学会
  • Place of Presentation: 第56回日本応用動物昆虫学会
• [Presentation] Metarhizium majusのシロテンハナムグリ病原性系統はタイワンカブトムシ由来の系統とは独立の系統である (2012)
  • Author(s): 西大海・飯山和弘・青木智佐・清水進
  • Organizer: 第56回日本応用動物昆虫学会
  • Place of Presentation: 第56回日本応用動物昆虫学会
• [Presentation] イモゾウムシ病原性Farinocystis様原虫の疫学的研究-1 (2011)
  • Author(s): 新見はるか・松山隆志・飯山和弘・青木智佐・清水進
  • Organizer: 第73回昆虫病理研究会
  • Place of Presentation: 東京大学農学部
  • Year and Date: 2011-12-03
• [Presentation] Pathogenicity of Metarhizium majus (Deuteromycotina; Hyphomycetes) to Scarabaeidae insect larvae (2011)
  • Author(s): Nishi O., Iiyama, K., Yasunaga-Aoki, C., and Shimizu, S
  • Organizer: The 8th International AFAS Joint Symposium between Japan and Korea
  • Place of Presentation: Yonago, Tottori
  • Year and Date: 2011-11-16
• [Presentation] 組換えセラリシン様メタロプロテアーゼの発現,精製の試み (2011)
  • Author(s): 河野康亮・飯山和弘・李在萬・日下部宜宏・青木智佐・清水進
  • Organizer: 日本蚕糸学会第65回東北支部・第62回関東支部・第77回関西支部・第67回九州支部合同大会
  • Place of Presentation: 岩手大学農学部
  • Year and Date: 2011-11-06
• [Presentation] The effect of pathogen odor signals on behavior of termites, Coptotermes formosanus (2011)
  • Author(s): Yanagawa, A., Fujiwara-Tsuji, N., Akino, T., Yoshimura, T., and Shimizu, S
  • Organizer: International Conference on Invertebrate Pathology and Microbial Control, OECD Symposium on Disease in Aquatic Crustaceans & 44th Annual Meeting of the Society for Invertebrate Pathology
  • Place of Presentation: Halifax, Nova Scotia, Canada
  • Year and Date: 2011-08-10
• [Presentation] Phylogenetic and pathogenic divergence within Metarhizium majus lineage International Conference on Invertebrate Pathology and Microbial Control (2011)
  • Author(s): Nishi O., Iiyama, K., Yasunaga-Aoki, C., and Shimizu, S
  • Organizer: OECD Symposium on Disease in Aquatic Crustaceans & 44th Annual Meeting of the Society for Invertebrate Pathology
  • Place of Presentation: Halifax, Nova Scotia, Canada
  • Year and Date: 2011-08-10
• [Presentation] シロアリを用いた釣り出し法による昆虫病原性糸状菌の土壌からの分離 (2010)
  • Author(s): 西 大海・飯山和弘・青木智佐・清水 進
  • Organizer: 日本蚕糸学会第64回東北支部・第66回中部支部・第62回東海支部・第76回関西支部・第66回九州支部合同大会 昆虫機能・利用学術講演会
  • Place of Presentation: 浦添市てだこホール 沖縄
  • Year and Date: 2010-11-15
• [Presentation] シロアリを用いた釣り出し法による昆虫病原性糸状菌の土壌からの分離 (2010)
  • Author(s): 西大海・飯山和弘・青木智佐・清水進
  • Organizer: 日本蚕糸学会第64回東北支部・第66回中部支部・第62回東海支部・第76回関西支部・第66回九州支部合同大会 昆虫機能・利用学術講演会
  • Place of Presentation: 浦添市てだこホール
  • Year and Date: 2010-11-15
• [Presentation] Serratia liquefaciens FK01セラリシン様メタロプロテアーゼ変異株のカイコに対する病原力の解析 (2010)
  • Author(s): 甲斐原布紗子・飯山和弘・青木智佐・清水進
  • Organizer: 日本蚕糸学会第64回東北支部・第66回中部支部・第62回東海支部・第76回関西支部・第66回九州支部合同大会 昆虫機能・利用学術講演会
  • Place of Presentation: 浦添市てだこホール
  • Year and Date: 2010-11-15
• [Presentation] イモゾウムシ病原性原虫の宿主幼虫個体への接種と増殖 (2010)
  • Author(s): 新見はるか・鶴田幸成・青木智佐・原口大・飯山和弘・清水進
  • Organizer: 日本蚕糸学会第64回東北支部・第66回中部支部・第62回東海支部・第76回関西支部・第66回九州支部合同大会 昆虫機能・利用学術講演会
  • Place of Presentation: 浦添市てだこホール
  • Year and Date: 2010-11-15
• [Presentation] トウモロコシからの昆虫病原性糸状菌の分離 (2010)
  • Author(s): 藤永小百合・堀下美穂・飯山和弘・青木智佐・清水進
  • Organizer: 日本蚕糸学会第64回東北支部・第66回中部支部・第62回東海支部・第76回関西支部・第66回九州支部合同大会 昆虫機能・利用学術講演会
  • Place of Presentation: 浦添市てだこホール
  • Year and Date: 2010-11-15
• [Presentation] Paenibaclllus popilliae var.popilliae Mameおよびvar.popilliae Sakuraのカタラーゼ (2010)
  • Author(s): 飯山和弘・浅野眞一郎・李在萬・日下部宜宏・青木智佐・清水進
  • Organizer: 平成22年度 蚕糸・昆虫機能利用学術講演会日本蚕糸学会第80回記念大会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 2010-04-04
• [Presentation] Serratia liquefaciens FK01のメタロプロテアーゼ遺伝子変異株の作出 (2010)
  • Author(s): 甲斐原布紗子・飯山和弘・青木智佐・清水進
  • Organizer: 平成22年度 蚕糸・昆虫機能利用学術講演会日本蚕糸学会第80回記念大会
  • Place of Presentation: 信州大学繊維学部
  • Year and Date: 2010-04-04
• [Presentation] 日本の土壌から分離されたMetarhizium属糸状菌のEF・1α遺伝子による系統解析 (2010)
  • Author(s): 西大海・飯山和弘・青木智佐・清水進
  • Organizer: 第54回日本応用動物昆虫学会大会
  • Place of Presentation: 北海道大学
  • Year and Date: 2010-03-27
• [Presentation] Isolation of entomopathogenic fungus Metarhizium anisopliaefrom soil by bait method with termite (2010)
  • Author(s): Nishi O., Iiyama, K., Yasunaga-Aoki, C., and Shimizu, S
  • Organizer: TRG 7, 2010 Pacific Rim Termite Research Group
  • Place of Presentation: Singapore
  • Year and Date: 2010-03-01
• [Presentation] Isolation of entomopathogenic fungus Metarhizium anisopliae from soil by bait method with termite. (2010)
  • Author(s): Oumi Nishi, Kazuhiro Iiyama, Chisa Yasunaga-Aoki, Susumu Shimizu
  • Organizer: TRG 7, 2010 Pacific Rim Termite Research Group
  • Place of Presentation: Singapore
  • Year and Date: 2010-03-01
• [Presentation] Paenibacillus popilliae var. popilliae Mameのカタラーゼ (2009)
  • Author(s): 飯山和弘・浅野眞一郎・李在萬・日下部宜宏・青木智佐・清水進
  • Organizer: 第71回昆虫病理研究会
  • Place of Presentation: つくば農林ホール
  • Year and Date: 2009-11-27
• [Presentation] Serratia liquefaciensの殺虫機構に関する研究 (2009)
  • Author(s): 甲斐原布紗子・飯山和弘・青木智佐・清水進
  • Organizer: 日本蚕糸学会第75回関西支部第・65回九州支部合同大会 昆虫機能・利用学術講演会
  • Place of Presentation: 山口大学
• [Presentation] Metarhizium属糸状菌の系統解析とその生態分布 (2009)
  • Author(s): 西大海・飯山和弘・青木智佐・清水進
  • Organizer: 日本蚕糸学会第75回関西支部第・65回九州支部合同大会 昆虫機能・利用学術講演会
  • Place of Presentation: 山口大学
• [Presentation] トウモロコシからの昆虫病原性糸状菌の分離の試み (2009)
  • Author(s): 堀下美穂・飯山和弘・青木智佐・清水進
  • Organizer: 日本蚕糸学会第75回関西支部第・65回九州支部合同大会 昆虫機能・利用学術講演会
  • Place of Presentation: 山口大学