Development of effective promoters and efficient systems for gene function analysis in insects

• Project/Area Number: 21380041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: SEZUTSU Hideki 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, ユニット長 (70342805)
• Co-Investigator(Kenkyū-buntansha): KOMOTO Natuo 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30355747) ; TOMITA Shuichiro 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (30360457) ; HATAKEYAMA Masatsugu 独立行政法人農業生物資源研究所, 昆虫成長制御研究ユニット, 主任研究員 (50281142) ; NIIMI Teruyuki 名古屋大学, 大学院生命農学研究科, 助教 (00293712)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
• Keywords: 昆虫分子生物学 / トランスジェニック昆虫 / プロモーター / カイコ / ナミテントウ / キイロショウジョウバエ / カブラハバチ / エンハンサートラップ / 部位特異的組換え / 人工プロモーター / トランスポゾン

Research Abstract

Systems to efficiently analyze gene function in insects were developed. Site-specific recombination works in silkworm and the system was found to be applicable for the clonal analysis and enhancer trapping. Gene editing utilizing artificial nucleases becomes possible. A promoter of baculovirus promoted expression of transgenes in some species belonging to different orders suggesting its utility across the insects.

Research Products

• [Journal Article] Tightly controlled Tetracycline-inducible transcription system for explosive gene expression in cultured silkworm cells (2013)
  • Author(s): Tatsuke T, Lee JM, Iiyama K, Sezutsu H, Uchino K, Kusakabe T
  • Journal Title: Archives of Insect Biochemistry & Physiology Volume: 82(4) Issue: 4 Pages: 173-182
  • DOI: 10.1002/arch.21083
• [Journal Article] Efficient disruption of endogenous Bombyx gene by TAL effector nucleases (2013)
  • Author(s): Sajwan S, Takasu Y, Tamura T, Uchino K, Sezutsu H, Zurovec M
  • Journal Title: Efficient disruption of endogenous Bombyx gene by TAL effector nucleases Volume: 43(1) Issue: 1 Pages: 17-23
  • DOI: 10.1016/j.ibmb.2012.10.011
• [Journal Article] A survey of the effectiveness of non-cell autonomous RNAi throughout development in the sawfly, Athalia rosae(Hymenoptera) (2013)
  • Author(s): Yoshiyama N, Tojo K, Hatakeyama M
  • Journal Title: Journal of Insect Physiology Volume: 59(4) Issue: 4 Pages: 400-407
  • DOI: 10.1016/j.jinsphys.2013.01.009
• [Journal Article] Dpp/BMP transport mechanism is required for wing venation in the sawfly Athalia rosae (2013)
  • Author(s): Matsuda S, Yoshiyama N, Künnapuu-Vulli J, Hatakeyama M, Shimmi O
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 43(5) Issue: 5 Pages: 466-473
  • DOI: 10.1016/j.ibmb.2013.02.008
  • Peer Reviewed
• [Journal Article] Precocious metamorphosis in the juvenile hormone-deficient mutant of the silkworm, Bombyx mori (2012)
  • Author(s): Daimon T., Sezutsu H. 他14名
  • Journal Title: PLoS Genetics Volume: 8 (3) Issue: 3 Pages: e1002486-e1002486
  • DOI: 10.1371/journal.pgen.1002486
• [Journal Article] A visible dominant marker for insect transgenesis. (2012)
  • Author(s): Osanai-Futahashi M, Ohde T, Hirata J, Uchino K, Futahashi R, Tamura T, Niimi T, Sezutsu H.
  • Journal Title: Nature Communications Volume: 3 Issue: 1 Pages: 1295-1295
  • DOI: 10.1038/ncomms2312
  • Peer Reviewed
• [Journal Article] PhiC31 integrase-mediated cassette exchange in silkworm embryos (2012)
  • Author(s): Yonemura N, Tamura T, Uchino K, Kobayashi I, Tatematsu K, Iizuka T, Sezutsu H, Muthulakshmi M, Nagaraju J, Kusakabe T
  • Journal Title: Molecular Genetics and Genomics Volume: 287(9) Issue: 9 Pages: 731-739
  • DOI: 10.1007/s00438-012-0711-y
  • Peer Reviewed
• [Journal Article] Identification of the Bombyx red egg gene reveals the involvement of a novel transporter family gene in the late steps of the insect ommochrome biosynthesis pathway (2012)
  • Author(s): Osanai-Futahashi M, Tatematsu KI, Yamamoto K, Narukawa J, Uchino K, Kayukawa T, Shinoda T, Banno Y, Tamura T, Sezutsu H.
  • Journal Title: Journal of Biological Chemistry Volume: 287(21) Issue: 21 Pages: 17706-17714
  • DOI: 10.1074/jbc.m111.321331
• [Journal Article] Development of a clonal analysis system in the silkworm, <i>Bombyx mori</i>, mediated by FLP and FRT (2012)
  • Author(s): Komoto N, Tomita S
  • Journal Title: Journal of Insect Biotechnology and Sericology Volume: 81 Issue: 2_3 Pages: 2_3_063-2_3_067
  • DOI: 10.11416/jibs.81.2_3_063
  • NAID: 130004464192
  • ISSN: 1346-8073, 1884-7978
• [Journal Article] A baculovirus immediate-early gene, ie1, promoter drives efficient expression of a transgene in both Drosophila melanogaster and Bombyx mori (2012)
  • Author(s): Masumoto, M., Ohde, T., Shiomi, K., Yaginuma, T. and Niimi, T.
  • Journal Title: PLoS ONE Volume: 7 Issue: 11 Pages: e49323-e49323
  • DOI: 10.1371/journal.pone.0049323
  • NAID: 120007100550
  • Peer Reviewed
• [Journal Article] SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells (2012)
  • Author(s): Kobayashi I, Tsukioka H, Komoto N, Uchino K, Sezutsu H, Tamura T, Kusakabe T, Tomita S
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 42 Issue: 2 Pages: 148-154
  • DOI: 10.1016/j.ibmb.2011.11.007
  • Peer Reviewed
• [Journal Article] An Efficient Binary System for Gene Expression in the Silkworm, Bombyx mori, Using GAL4 Variants (2011)
  • Author(s): Kobayashi I., Sezutsu H. 他10名
  • Journal Title: Archives of Insect Biochemistry & Physiology Volume: 76 Issue: 4 Pages: 195-210
  • DOI: 10.1002/arch.20402
  • Peer Reviewed
• [Journal Article] Rescue of the Aojuku white-egg translucent (<i>w-3</i>^<i>ol</i>) <i>Bombyx mori</i> mutant by transgenic expression of the wild-type <i>Bmwh3</i> gene (2011)
  • Author(s): Kobayashi, I. 他6名
  • Journal Title: Journal of Insect Biotechnology and Sericology Volume: 79 Issue: 3 Pages: 3_111-3_116
  • DOI: 10.11416/jibs.79.3_111
  • NAID: 130004464171
  • ISSN: 1346-8073, 1884-7978
• [Journal Article] A single sex pheromone receptor determines chemical response specificity of sexual behavior in the silkmoth Bombyx mori (2011)
  • Author(s): Sakurai, T., et al
  • Journal Title: PLoS Genetics Volume: 7 Issue: 6 Pages: 1-10
  • DOI: 10.1371/journal.pgen.1002115
  • Peer Reviewed
• [Journal Article] テントウムジの斑紋形成メカニズムと進化 (2011)
  • Author(s): 新美輝幸・岡田駿吾・大場裕一・柳沼利信
  • Journal Title: 昆虫と自然 Volume: 46 Pages: 5-9
• [Journal Article] Positional cloning of silkworm white egg 2 (w-2) locus reveals functional conservation and diversification of ABC transporters for pigmentation in insects (2011)
  • Author(s): Tatematsu, K.I., et al.
  • Journal Title: Genes to Cells Volume: 16 Pages: 331-342
  • Peer Reviewed
• [Journal Article] チョウ目害虫とカイコのゲノム構造の類似性 (2011)
  • Author(s): 瀬筒秀樹
  • Journal Title: 植物防疫 Volume: 65 Pages: 14-17
• [Journal Article] Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection (2010)
  • Author(s): Takasu, Y. 他8名
  • Journal Title: Insect Biochemistry and Molecular Biology Volume: 40 Issue: 10 Pages: 759-765
  • DOI: 10.1016/j.ibmb.2010.07.012
• [Journal Article] Extensive synteny conservation of holocentric chromosomes in Lepidoptera despite high rates of local genome rearrangement (2010)
  • Author(s): d'Alencon, E., Sezutsu, H., et al.
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Issue: 17 Pages: 7680-7685
  • DOI: 10.1073/pnas.0910413107
  • NAID: 40018728414
• [Journal Article] Transgenic analysis of the BmBLOS2 gene that governs the translucency of the larval integument of the silkworm, Bombyx mori (2010)
  • Author(s): Fujii, T. 他7名
  • Journal Title: Insect Molecular Biology Volume: 19 Issue: 5 Pages: 659-667
  • DOI: 10.1111/j.1365-2583.2010.01020.x
• [Journal Article] The Silkworm-An Attractive BioResource Supplied by Japan (2010)
  • Author(s): Banno Y., Shimada T., Kajiura Z., Sezutsu H
  • Journal Title: Experimental Animals Volume: 59 Issue: 2 Pages: 139-146
  • DOI: 10.1538/expanim.59.139
  • NAID: 130000254341
  • ISSN: 0007-5124, 1341-1357, 1881-7122
• [Journal Article] Transgenic tools for members of the genus drosophila with sequenced genomes (2010)
  • Author(s): Holtzman S., Miller D., Eisman,R.C., Kuwayama H.,Niimi T., Kaufman T.C
  • Journal Title: Fly Volume: 4 Issue: 4 Pages: 349-362
  • DOI: 10.4161/fly.4.4.13304
• [Journal Article] Transgenic tools for members of the genus drosophila with sequenced genomes (2010)
  • Author(s): Holtzman, S., et al.
  • Journal Title: Fly Volume: 4 Pages: 1-13
  • Peer Reviewed
• [Journal Article] Characterization of abdominal appendages in the sawfly, Athalia rosae (Hymenoptera), by morphological and gene expression analyses (2010)
  • Author(s): Oka, K., et al.
  • Journal Title: Development Genes and Evolution Volume: 220 Pages: 53-59
  • Peer Reviewed
• [Journal Article] A CD36-related transmembrane protein is coordinated with an intracellular lipid-binding protein in selective carotenoid transport for cocoon coloration (2010)
  • Author(s): Sakudoh T., et al.
  • Journal Title: Journal of Biological Chemistry 63(8) Pages: 7738-7751
  • Peer Reviewed
• [Journal Article] KAIKObase: An integrated silkworm genome database and data mining tool (2009)
  • Author(s): Shimomura M. 他14名
  • Journal Title: BMC Genomics Volume: 10 Issue: 1 Pages: 486-486
  • DOI: 10.1186/1471-2164-10-486
• [Journal Article] The sawfly, Athalia rosae ruficornis (Hymenoptera) as a model insect for developmental and reproductive biology: what has been done and what should be done? Proc. Arthropod. Embryol (2009)
  • Author(s): Hatakeyama M., et al.
  • Journal Title: Soc. Jpn, Volume: 44 Pages: 1-12
  • URL: http://aesj.co-site.jp/paesj.html
• [Journal Article] The sawfly, Athalia rosae ruficornis (Hymenoptera) as a model insect for developmental and reproductive biology : what has been done and what should be done? (2009)
  • Author(s): Hatakeyama M., et al.
  • Journal Title: Proc.Arthropod.Embryol.Soc.Jpn. 44 Pages: 1-12
  • Peer Reviewed
• [Journal Article] 遺伝子組換え技術を使った害虫防除 (2009)
  • Author(s): 畠山正統
  • Journal Title: 植物防疫 63(8) Pages: 490-493
• [Journal Article] Expression of the Japanese oak silkworm Antheraea yamamai fibroin gene in the domesticated silkworm Bombyx mori (2009)
  • Author(s): Kobayashi I., et al.
  • Journal Title: Insect Science 16(6) Pages: 465-473
  • Peer Reviewed
• [Journal Article] KAIKObase : An integrated silkworm genome database and data mining tool (2009)
  • Author(s): Shimomura M., et al.
  • Journal Title: Genomics 10 Pages: 486-486
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): 畠山正統
  • Journal Title: 分子昆虫学(第7章4:遺伝子組換え技術の害虫防除への利用)(共立出版) Pages: 394-396
• [Journal Article] (2009)
  • Author(s): 瀬筒秀樹、冨田正浩
  • Journal Title: 分子昆虫学(第1章6:遺伝子組換え技術)(共立出版) Pages: 37-39
• [Presentation] Athalia rosae (2012)
  • Author(s): Hatakeyama M
  • Organizer: an emerging genetic model system for sawflies XXIV International Congress of Entomology: S1107TH07,
  • Place of Presentation: Daegu, Korea
  • Year and Date: 2012-08-23
• [Presentation] Transgenic silkworms for analysis of gene function and production of recombinant proteins/silks (2012)
  • Author(s): Sezutsu H, Tsubota T, Osanai-Futahashi M, Suzuki T, Tatematsu KI, Kobayashi I, Iizuka T, Uchino K, Yonemura N, Takasu Y, Tamura T
  • Organizer: ICE2012 - XXIV International Congress of Entomology2012
  • Place of Presentation: Korea
  • Year and Date: 2012-08-20
• [Presentation] Pigment synthesis pathway genes and their application to visible transgenic markers (2012)
  • Author(s): Osanai-Futahashi M, Tatematsu KI, Yamamoto K, Uchino K, Futahashi R, Tamura T, Sezutsu H
  • Organizer: ICE2012 - XXIV International Congress of Entomology 2012
  • Place of Presentation: Korea
  • Year and Date: 2012-08-20
• [Presentation] Genetically modified silkworms and silks - recent updates and future prospects (2012)
  • Author(s): Sezutsu H, Iizuka T, Uchino K, Kobayashi I, Tatematsu KI, Tsubota T, Yonemura N, Okada E, Nakajima K, Machii H, Tamura T
  • Organizer: The 7th International Conference on Wild Silkmoth and Silk
  • Place of Presentation: Mahasarakham Univ., Thailand
• [Presentation] Transgenic silkworms for analysis of gene function and production of recombinant proteins/silks (2012)
  • Author(s): Sezutsu H, Tsubota T, Osanai-Futahashi M, Suzuki T, Tatematsu KI, Kobayashi I, Iizuka T, UchinoK, Yonemura N, Takasu Y, Tamura T
  • Organizer: ICE2012 - XXIV International Congress of Entomology 2012
  • Place of Presentation: Daegu, Korea
  • Invited
• [Presentation] Genetically modified silkworms and silks - recent updates and future prospects (2012)
  • Author(s): Sezutsu H, Iizuka T, Uchino K, Kobayashi I, Tatematsu KI, Tsubota T, Yonemura N, Okada E, Nakajima K, Machii H, Tamura T
  • Organizer: The 7th International Conference on Wild Silkmoth and Silk
  • Place of Presentation: Mahasarakham, Thailand
  • Invited
• [Presentation] チョウ目昆虫ゲノム情報と遺伝子組換えカイコの利用 (2012)
  • Author(s): 瀬筒秀樹
  • Organizer: 第 10 回農薬バイオサイエンス研究会シンポジウム「農薬科学の将来を支える新技術・新知見」
  • Place of Presentation: 東京農業大学, 東京
  • Invited
• [Presentation] Pigment synthesis pathway genes and their application to visible transgenic markers (2012)
  • Author(s): Osanai-Futahashi M, Tatematsu KI, Yamamoto K, Uchino K, Futahashi R, Tamura T, Sezutsu H
  • Organizer: ICE2012 - XXIV International Congress of Entomology 2012
  • Place of Presentation: Daegu, Korea
• [Presentation] カブラハバチにおけるmRNAシークエンスを利用した遺伝子の探索と同定 (2012)
  • Author(s): 畠山正統, 炭谷めぐみ, 関根一希
  • Organizer: 第48回日本節足動物発生学会
  • Place of Presentation: 菅平, 長野
• [Presentation] Athalia rosae: an emerging genetic model system for sawflies (2012)
  • Author(s): Hatakeyama M
  • Organizer: ICE2012 - XXIV International Congress of Entomology 2012
  • Place of Presentation: Daegu, Korea
  • Invited
• [Presentation] Towards the understanding of sex determination in Coleoptera (2011)
  • Author(s): Niimi, T.
  • Organizer: Nothiger Meeting
  • Place of Presentation: University of Amsterdam, Amsterdam, The Netherlands
  • Year and Date: 2011-10-06
• [Presentation] Transgenic ladybird: development of gene function analysis systems for non-model insects (2011)
  • Author(s): Niimi, T
  • Organizer: The 1st International Conference on the Criket / RNAi Symposium for Medicine-Agriculture-Engineering Collaboration Project (Joint Meeting)
  • Place of Presentation: (要旨集)
  • Year and Date: 2011-03-14
• [Presentation] Transgenic ladybird : development of gene function analysis systems for non-model insects. (2011)
  • Author(s): Niimi, T.
  • Organizer: The 1st International Conference on the Criket /RNAi Symposium for Medicine-Agriculture-Engineering Collaboration Project (Joint Meeting)
  • Place of Presentation: 要旨集
  • Year and Date: 2011-03-14
• [Presentation] Development of transgenic silkworm Bombyx mori for functional genomics (2011)
  • Author(s): Sezutsu, H. et al.
  • Organizer: Sixth International Symposium on Molecular Insect Science
  • Place of Presentation: Sixth International Symposium on Molecular Insect Science
• [Presentation] Development of transgenic silkworms for functional genomics in Bombyx mori as a lepidopteran model insect 5th Annual Arthropod Genomics Symposium (2011)
  • Author(s): Sezutsu, H. et al.
  • Organizer: 5th Annual Arthropod Genomics Symposium
  • Place of Presentation: Kansas, USA
• [Presentation] Transgenic silkworm as a tool for functional analysis of insect genes and for bioreactor (2011)
  • Author(s): Tamura, T. et al.
  • Organizer: 2nd International Symposium on Silkworm Functional Genomics and Modern Silk Road
  • Place of Presentation: Chongqing, China
• [Presentation] Development of transgenic silkworm bombyx mori for functional genomics (2011)
  • Author(s): Sezutsu, H., et al
  • Organizer: Sixth International Symposium on Molecular Insect Science
  • Place of Presentation: Amsterdam, The Netherland
• [Presentation] Identification of a novel ommochrome pigmentation gene in lepidopteran and coleopteran insects (2011)
  • Author(s): Osanai-Futahashi, M., et al
  • Organizer: Sixth International Symposium on Molecular Insect Science
  • Place of Presentation: Amsterdam, The Netherland
• [Presentation] Extensive synteny conservation of holocentric chromosomes in Lepidoptera despite high rates of local genome rearrangement (2010)
  • Author(s): d'Alencon, E., et al.
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-11
• [Presentation] カブラハバチにおけるプロモーター探索の試み (2010)
  • Author(s): 畠山正統
  • Organizer: 第31回菅平動物学セミナー
  • Place of Presentation: 長野・筑波大菅平高原実験センター
  • Year and Date: 2010-12-04
• [Presentation] カブラハバチにおけるプロモーター探索の試み (2010)
  • Author(s): 畠山正統
  • Organizer: 第31回菅平動物学セミナー
  • Place of Presentation: 長野・菅平(筑波大学菅平高原実験センター)
  • Year and Date: 2010-12-04
• [Presentation] カブラハバチの付属肢形成に関わる遺伝子の解析 (2010)
  • Author(s): 吉山直利, ら
  • Organizer: 第31回菅平動物学セミナー
  • Place of Presentation: 長野・菅平(筑波大学菅平高原実験センター)
  • Year and Date: 2010-12-04
• [Presentation] Directional transport of Dpp/BMP draws diversified wing vein patterns in insects (2010)
  • Author(s): Matsuda, S., et al.
  • Organizer: The 16^<th> International Conference on the International Society of Differentiation
  • Place of Presentation: Nara, Japan
  • Year and Date: 2010-11-17
• [Presentation] Development of transposon mutagenesis systems and a database for transposon insertion lines in silkworm Bombyx mori (2010)
  • Author(s): Sezutsu, H. et al.
  • Organizer: International Symposium; New Silk Road: Silkworm Genome to Sustainable Agriculture
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2010-11-10
• [Presentation] Development of transgenic silkworm as a tool for functional analysis and for bioreactor (2010)
  • Author(s): Tamura, T. et al.
  • Organizer: International Symposium; New Silk Road: Silkworm Genome to Sustainable Agriculture
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2010-11-10
• [Presentation] Development of transposon mutagenesis systems and a database for transposon insertion lines in silkworm Bombyx mori (2010)
  • Author(s): Sezutsu, H., et al.
  • Organizer: International Symposium ; New Silk Road : Silkworm Genome to Sustainable Agriculture
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2010-11-10
• [Presentation] Development of transgenic silkworm as a tool for functional analysis and for bioreactor (2010)
  • Author(s): Tamura, T., et al.
  • Organizer: International Symposium ; New Silk Road : Silkworm Genome to Sustainable Agriculture
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2010-11-10
• [Presentation] カブラハバチの翅脈形成におけるdecapentaplegicおよびtwisted gastrulation遺伝子の機能 (2010)
  • Author(s): 吉山直利, ら
  • Organizer: 第46回日本節足動物発生学会
  • Place of Presentation: 福島・甲子
  • Year and Date: 2010-06-12
• [Presentation] Extensive synteny conservation of holocentric chromosomes in lepidoptera despite high rates of local genome rearrangement (2010)
  • Author(s): Sezutsu, H. et al.
  • Organizer: Workshop on Recent Advances in Sericulture Research
  • Place of Presentation: Bengalure, India
  • Year and Date: 2010-05-18
• [Presentation] Transgenic silkworm as a tool for the analysis of gene function and bioreactor (2010)
  • Author(s): Tamura, T. et al.
  • Organizer: Workshop on Recent Advances in Sericulture Research
  • Place of Presentation: Bengalure, India
  • Year and Date: 2010-05-18
• [Presentation] テントウムシをめぐる擬態斑紋 (2010)
  • Author(s): 新美輝幸
  • Organizer: 愛知学院大学歯学部 第6回若手研究者・大学院生のための講演会
  • Place of Presentation: 名古屋市、愛知学院大学歯学部
  • Year and Date: 2010-04-20
• [Presentation] 新規ミューテーターを用いたカイコ・エンハンサートップ系統の作出と解析, (2010)
  • Author(s): 内野恵郎他
  • Organizer: 平成22年度蚕糸・昆虫機能学術講演会.日本蚕糸学会第80回大会
  • Place of Presentation: 上田市、信州大学
  • Year and Date: 2010-04-04
• [Presentation] 新規ミューテーターを用いたカイコ・エンハンサートップ系統の作出と解析 (2010)
  • Author(s): 内野恵郎, ら
  • Organizer: 平成22年度蚕糸・昆虫機能学術講演会-日本蚕糸学会第80回大会-
  • Place of Presentation: 上田市、信州大学
  • Year and Date: 2010-04-04
• [Presentation] 鞘翅目昆虫の性決定遺伝子の解析 (2010)
  • Author(s): 西河秀人, ら
  • Organizer: 平成22年度蚕糸・昆虫機能学術講演会-日本蚕糸学会第80回大会-
  • Place of Presentation: 上田市、信州大学繊維学部
  • Year and Date: 2010-04-04
• [Presentation] Construction of an enhancer trap system and Bombyx Trap DataBase for the analysis of gene function in silkworm Bombyx mori (2009)
  • Author(s): Sezutsu H., et al.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-11
• [Presentation] ナミテントウにおける性決定遺伝子の解析 (2009)
  • Author(s): 西河秀人・柳沼利信・新美輝幸
  • Organizer: 日本蚕糸学会中部支部第65回・東海支部大会第61回
  • Place of Presentation: 名古屋大学 野依記念学術交流館・会議室
  • Year and Date: 2009-12-04
• [Presentation] 形質転換昆虫におけるBmNPVie1プロモーターの汎用性の検討 (2009)
  • Author(s): 大出高弘・増本三香・柳沼利信・新美輝幸
  • Organizer: 日本蚕糸学会中部支部第65回・東海支部大会第61回
  • Place of Presentation: 名古屋大学野依記念学術交流館・会議室
  • Year and Date: 2009-12-03
• [Presentation] 形質転換昆虫におけるBmNPVielプロモーターの汎用性の検討 (2009)
  • Author(s): 大出高弘・増本三香・柳沼利信・新美輝幸
  • Organizer: 日本蚕糸学会中部支部第65回・東海支部大会第61回
  • Place of Presentation: 名古屋大学 野依記念学術交流館・会議室
  • Year and Date: 2009-12-03
• [Presentation] カイコ遺伝子組換え技術の開発 (2009)
  • Author(s): 瀬筒秀樹
  • Organizer: 公開シンポジウム「天の虫『蚕(かいこ)』、新たな可能性への挑戦」
  • Place of Presentation: つくばエポカル
  • Year and Date: 2009-11-18
• [Presentation] 鞘翅目昆虫の性決定機構の解明を目指して (2009)
  • Author(s): 西河秀人・梶村恒・柳沼利信・新美輝幸
  • Organizer: 昆虫ワークショップ09福岡
  • Place of Presentation: ウェルサンピア福岡
  • Year and Date: 2009-10-30
• [Presentation] Transgenic silkworm as a tool for the analysis of gene function.International Symposium on Bombyx mori (2009)
  • Author(s): Sezutsu H., et al.
  • Organizer: Functional Genomics and Modern Silk Road
  • Place of Presentation: Chongqing, China
  • Year and Date: 2009-10-23
• [Presentation] Transgenic silkworm as a tool for the analysis of gene function. (2009)
  • Author(s): Sezutsu H., et al.
  • Organizer: International Symposium on Bombyx mori. Functional Genomics and Modern Silk Road.
  • Place of Presentation: Chongqing, China
  • Year and Date: 2009-10-23
• [Presentation] Development of transgenic silkworm Bombyx mori for functional genomics (2009)
  • Author(s): Sezutsu H., et al.
  • Organizer: The 6th Asia-Pacific Congress of Entomology
  • Place of Presentation: Beijing, China
  • Year and Date: 2009-10-20
• [Presentation] ナミテントウにおける性決定遺伝子の探索 (2009)
  • Author(s): 西河秀人・佐原健・柳沼利信・新美輝幸
  • Organizer: 2009年度 昆虫ポストゲノム研究会
  • Place of Presentation: 北海道大学 農学部総合研究棟
  • Year and Date: 2009-09-11
• [Presentation] トランスジェニックカイコを用いた遺伝子機能解析 (2009)
  • Author(s): 瀬筒秀樹
  • Organizer: 2009年度 昆虫ポストゲノム研究会
  • Place of Presentation: 北海道大学 農学部総合研究棟
  • Year and Date: 2009-09-10
• [Presentation] トランスジェニックカイコおよびトランスジェニックフライにおけるBmNPVie1プロモーターのレポーターアッセイ (2009)
  • Author(s): 大出高弘・増本三香・柳沼利信・新美輝幸
  • Organizer: 2009年度 昆虫ポストゲノム研究会
  • Place of Presentation: 北海道大学 農学部総合研究棟
  • Year and Date: 2009-09-10
• [Presentation] トランスジェニックカイコおよびトランスジェニックフライにおけるBmNPVielプロモーターのレポーターアッセィ (2009)
  • Author(s): 大出高弘・増本三香・柳沼利信・新美輝幸
  • Organizer: 2009年度 昆虫ポストゲノム研究会
  • Place of Presentation: 北海道大学 農学部総合研究棟
  • Year and Date: 2009-09-10
• [Presentation] Construction of enhancer trap system and Bombyx Trap DataBase for the analysis of gene function in silkworm Bombyx mori (2009)
  • Author(s): Sezutsu H., et al.
  • Organizer: The Eighth International Workshop on MOLECULAR BIOLOGY AND GENETICS OF THE LEPIDOPTERA
  • Place of Presentation: Crete, Greece
  • Year and Date: 2009-08-28
• [Presentation] 遺伝子組換え技術を使った害虫防除 (2009)
  • Author(s): 畠山正統
  • Organizer: 公開シンポジウム「ポストゲノム時代の害虫防除研究のあり方」第1回 昆虫ゲノム情報と総合的害虫管理技術IPM
  • Place of Presentation: 秋葉原コンベンションセンター、東京
  • Year and Date: 2009-04-24
• [Presentation] Facilitated transport and active retention of BMP draw diversified wing vein patterns in insects (2009)
  • Author(s): Matsuda S., et al.
  • Organizer: 16th International Society of Developmental Biologists Congress
  • Place of Presentation: Edinburgh, UK
• [Presentation] Construction of enhancer trap system and Bombyx Trap DataBase for the analysis of gene function in silkworm Bombyx mori
  • Author(s): Sezutsu H., et al.
• [Book] 分子昆虫学 (第7章4 : 遺伝子組換え技術の害虫防除への利用) (2009)
  • Author(s): 畠山正統
  • Publisher: 共立出版
• [Book] 分子昆虫学 (第1章6 : 遺伝子組換え技術) (2009)
  • Author(s): 瀬筒秀樹、冨田正浩
  • Publisher: 瀬筒秀樹、冨田正浩