Evolution of W, female-specific sex chromosome through the comparative genomics between Bombyx mori and B. mandarina

• Project/Area Number: 21380042
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: KADONO-OKUDA Keiko 独立行政法人農業生物資源研究所, 昆虫ゲノム研究ユニット, 上級研究員 (40355722)
• Co-Investigator(Kenkyū-buntansha): MITA Kazuei (独)農業生物資源研究所, 昆虫科学研究領域, 特任上級研究員 (30159165) ; ABE Hiroaki 東京農工大学, 大学院・共生科学技術研究院, 助教 (80222660) ; MAEKAWA Hideaki 琉球大学, 熱帯生物圏研究センター, 教授 (60100096) ; NAKAJIMA Yumiko 琉球大学, 熱帯生物圏研究センター, 准教授 (70244340) ; YUKUHIRO Kenji (独)農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, 主任研究員 (50343992)
• Co-Investigator(Renkei-kenkyūsha): SHIMADA Toru 東京大学, 農学生命科学研究科, 教授 (20202111)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000); Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
• Keywords: 養蚕 / 蚕糸 / クワコ / カイコ / 進化 / 性染色体 / W染色体 / トランスポゾン / 性決定 / Bombyx / ゲノム / Z / W

Research Abstract

We analyzed the W-chromosome construction in B. mori, B. mandarina and other related species. B. mandarina and B. huttoni was branched 14 million years ago(MYA). In B. mandarina one copy set of two genes was translocated from an autosome to W-chromosome(z1-z20) in 7 MYA, and z1-z20 was duplicated on W-chromosome 0.3 MYA. Such a new type of B. mandarina containing duplicated z1-z20 on W-chromosome had spread drastically. But, since Japan islands have been isolated from the continent 0.1 MYA, the new type did not invade in Japan. In present, there are 4 copies of z1-z20 in all B. mori, and B. mandarina except for Japan.

Research Products

• [Journal Article] Female sex pheromone and male behavioral responses of the bombycid moth Trilocha varians : comparison with those of the domesticated silkmoth Bombyx mori (2012)
  • Author(s): Daimon T, Fujii T, Yago M, Hsu Y-F, Nakajima Y, Fujii T, Katsuma S, Ishikawa Y, and Shimada T
  • Journal Title: Naturwissenschaften Volume: 99 Pages: 207-15
  • Peer Reviewed
• [Journal Article] Molecular phylogeny, laboratory rearing, and karyotype of the bombycid moth (2012)
  • Author(s): Daimon T, Yago M, Hsu Y-F., Fujii T, Nakajima Y, Kokusho R, Abe H, Katsuma S, Shimada T
  • Journal Title: Trilocha varians.、Journal of Insect Science Volume: 12
  • Peer Reviewed
• [Journal Article] The silkworm W chromosome is a source of female-enriched piRNAs (2012)
  • Author(s): Kawaoka S, Kadota K, Arai Y, Suzuki Y, Fujii T, Abe H, Yasukochi Y, Mita K, Sugano S, Shimizu K, Tomari Y, Shimada T, Katsuma Shimada, T.
  • Journal Title: RNA Volume: 17 Pages: 2144-51
  • Peer Reviewed
• [Journal Article] Female sex pheromone and male behavioral responses of the bombycid moth Trilocha varians : comparison with those of the domesticated silkmoth Bombyx mori (2012)
  • Author(s): Daimon, T., Fujii, T., Yago, M., Hsu, Y.-F., Nakajima, Y., Fujii, T., Katsuma, S., Ishikawa, Y., Shimada, T.
  • Journal Title: Naturwissenschaften Volume: 99 Pages: 207-215
  • Peer Reviewed
• [Journal Article] Molecular phylogeny, laboratory rearing, and karyotype of the bombycid moth, Trilocha varians (2012)
  • Author(s): Daimon, T., Yago, M., Hsu, Y.-F., Fujii, T., Nakajima, Y., Kokusho, R., Abe, H., Katsuma, S., Shimada, T.
  • Journal Title: Journal of Insect Science Volume: 12 Pages: 1-17
  • Peer Reviewed
• [Journal Article] 南西諸島に棲息する昆虫・クモ類における転移因子マリナー様配列(MLE : mariner-like element)の比較系統解析 (2011)
  • Author(s): 山田香織,川西祐一,奥間政一郎,岩崎華子,山田明徳.佐々木健志,中島裕美子,前川秀彰
  • Journal Title: Entomotech Volume: 35 Pages: 13-39
  • Peer Reviewed
• [Journal Article] 分子系統解析によるクワコの進化と日本列島の地理的変動との関係-カイコのゲノム情報とクワコの多型から- (2010)
  • Author(s): 川西祐一、前川秀彰、中島裕美子
  • Journal Title: 蚕糸・昆虫バイオテック Volume: 79 Pages: 109-117
  • NAID: 10029378271
• [Journal Article] Novel non-autonomous transposable elements on W chromosome of the silkworm (2010)
  • Author(s): Abe H, Fujii T, Shimada T, Mita K.
  • Journal Title: Bombyx mori., Journal of Genetics Volume: 89 Pages: 375-87
  • Peer Reviewed
• [Journal Article] Molecular analysis of sex chromosome-linked mutants in the silkworm Bombyx mori (2010)
  • Author(s): Fujii T, Abe H, Shimada T.
  • Journal Title: Journal of Genetics Volume: 53 Pages: 585-93
  • Peer Reviewed
• [Journal Article] Recent transposition of yabusame, a novel piggyBac-like transposable element in the genome of the silkworm, Bombyx mori (2010)
  • Author(s): Daimon T, Mitsuhiro M, Katsuma S, Abe H, Mita K, Shimada T.
  • Journal Title: Genome Volume: 53 Pages: 585-93
  • Peer Reviewed
• [Journal Article] Repression of tyrosine hydroxylase is responsible for the sex-linked chocolate mutation of the silkworm, Bombyx mori (2010)
  • Author(s): Liu C, Yamamoto K, Cheng TC, Kadono-Okuda K, Narukawa J, Liu SP, Han Y, Futahashi R, Kidokoro K, Noda H, Kobayashi I, Tamura T, Ohnuma A, Banno Y, Dai FY, Xiang ZH, Goldsmith MR, Mita K, Xia QY
  • Journal Title: Proc Natl Acad Sci U S A Volume: 107 Pages: 12980-5
  • Peer Reviewed
• [Journal Article] 亜熱帯に生息する昆虫・クモ類に見出される転移因子マリナー様配列の水平伝播機構の解析 (2010)
  • Author(s): 奥間政一郎,川西祐一,佐々木健志,日高道雄,前川秀彰,中島裕美子
  • Journal Title: Entomotech Volume: 34 Pages: 47-52
  • URL: http://www.agr.kyushu-u.ac.jp/sanshi-kyushu/entomotech34.html
  • Peer Reviewed
• [Journal Article] Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable(spli) larval body phenotype in the silkworm (2010)
  • Author(s): Fujii T, Kuwazaki S, Yamamoto K, Abe H, Ohnuma A, Katsuma S, Mita K, Shimada T.
  • Journal Title: Bombyx mori. Genome Volume: 53 Pages: 45-54
  • Peer Reviewed
• [Journal Article] Identification of 2nd chromosome region translocated onto the W chromosome by RFLP with EST-cDNA clones in the Gensei-kouken strains of the mulberry silkworm (2010)
  • Author(s): Sivaramakurup Sreekumar, Keiko Kadono-Okuda, Ken-ichi Nagayasu and Wajiro Hara
  • Journal Title: Bombyx mori L、Genetics and Molecular Biology Volume: 33 Pages: 27-35
  • Peer Reviewed
• [Journal Article] Novel non-autonomous transposable elements on W chromosome of the silkworm, Bombyx mori (2010)
  • Author(s): Abe H, Fujii T, Shimada T, Mita K
  • Journal Title: Journal of Genetics Volume: 89 Pages: 375-87
  • Peer Reviewed
• [Journal Article] Molecular analysis of sex chromosome-linked mutants in the silkworm Bombyx mori (2010)
  • Author(s): Fujii T, Abe H, Shimada T
  • Journal Title: Journal of Genetics Volume: 89 Pages: 365-74
  • Peer Reviewed
• [Journal Article] 亜熱帯に生息する昆虫・クモ類に見出される転移因子マリナー様配列の水平伝播機構の解析 (2010)
  • Author(s): 奥間政一郎, 川西祐一, 佐々木健志, 日高道雄, 前川秀彰, 中島裕美子
  • Journal Title: Entomotech 34(in press)
  • Peer Reviewed
• [Journal Article] Identification and molecular characterization of a sex chromosome rearrangement causing a soft and pliable (spli) larval body phenotype in the silkworm, Bombyx mori. (2010)
  • Author(s): Fujii T, Kuwazaki S, Yamamoto K, Abe H, Ohnuma A, Katsuma S, Mita K, Shimada T.
  • Journal Title: Genome 53(1) Pages: 45-54
  • Peer Reviewed
• [Journal Article] Identification of 2nd chromosome region translocated onto the W chromosome by RFLP with EST-cDNA clones in the Gensei-kouken strains of the mulberry silkworm, Bombyx mori L (2010)
  • Author(s): Sivaramakurup Sreekumar, Keiko Kadono-Okuda, Ken-ichi Nagayasu, Wajiro Hara
  • Journal Title: Genetics and Molecular Biology 33(1) Pages: 27-35
  • Peer Reviewed
• [Presentation] カイコおよび近縁野蚕ゲノムにおけるBm1について (2012)
  • Author(s): 味村正博、三田和英, 他
  • Organizer: クワコ談話会
  • Place of Presentation: 琉球大学
• [Presentation] カイコ濃核病ウイルスと抵抗性突然変異の起源 (2012)
  • Author(s): 門野敬子、味村正博、三田和英、中島裕美子、前川秀彰、行弘研司、阿部広明, 他
  • Organizer: クワコ談話会
  • Place of Presentation: 琉球大学
• [Presentation] 日本産クワコの系統地理学 (2012)
  • Author(s): 行弘研司, 他
  • Organizer: クワコ談話会
  • Place of Presentation: 琉球大学
• [Presentation] カイコとクワコのBmTNML座位における、転移因子の動向 (2012)
  • Author(s): 中島裕美子, 他
  • Organizer: クワコ談話会
  • Place of Presentation: 琉球大学
• [Presentation] 日本に生息するクワコ自然集団間の遺伝的変異 (2012)
  • Author(s): 行弘研司、河本夏雄、冨田秀一郎、瀬筒秀樹、田村俊樹、小瀬川英一、木内信、門野敬子、三田和英、味村正博、橋本拓真、伊藤雅信
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学
• [Presentation] バキュロウイルスをベクターとしたトランスポゾン転移実験系の構築 (2012)
  • Author(s): Gurung Raj、川西祐一、山田明徳、片岡周子、松浦善治、中島裕美子、前川秀彰
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学
• [Presentation] イチジクカサンのW染色体RAPDマーカーの探索 (2012)
  • Author(s): 惣中祐季、阿部広明、普後一、藤井告、嶋田透、大門高明
  • Organizer: 日本蚕糸学会第82回大会
  • Place of Presentation: 九州大学
• [Presentation] Molecular genetics of the densovirus resistance genes in Bombyx silkworms (2011)
  • Author(s): K. Kadono-Okuda, D. O. Ogoyi, H. Masui, K. Kidokoro, M. Ajimura, K. Ito, K. Yukuhiro, E. Kosegawa, Y. Banno, H. Maekawa, Y. Nakajima, K. Morimoto, M. Ichida, T. Adachi, K. Yamamoto, K. Mita
  • Organizer: 22nd Congress of the International Sericultural Commission
  • Place of Presentation: Chiangmai(タイ)
• [Presentation] (パスツール賞受賞講演) Silk Genome Opens New Silk Road for Insect Sciences and Its Applications (2011)
  • Author(s): K. Mita
  • Organizer: 22nd Congress of the International Sericultural Commission
  • Place of Presentation: Chiangmai(タイ)
• [Presentation] Ancient Mariner Transposon Targeted by Other Transposable Elements in the Genome of Bombyx mori and B. mandarina (2011)
  • Author(s): Nakagome, S., Mano, S., Nakajima, Y.
  • Organizer: Annual Meeting of the Society for Molecular Biology and Evolution
  • Place of Presentation: 京都大学
• [Presentation] Ancient Mariner Transposon Targeted by Other Transposable Elements in the Genome of Bombyx mori and B.mandarina (2011)
  • Author(s): Nakagome, S., Mano, S., Nakajima, Y.
  • Organizer: Annual Meeting of the Society for Molecular Biology and Evolution
  • Place of Presentation: 京都大学
• [Presentation] Silk Genome Opens New Silk Road for Insect Sciences and Its Applications (2011)
  • Author(s): K.Mita
  • Organizer: 22nd Congress of the International Sericultural Commission
  • Place of Presentation: Chiangmai(タイ)(パスツール賞受賞講演)
• [Presentation] カイコ濃核病ウイルス抵抗性遺伝子、nsd-1とnsd-2の分布 (2010)
  • Author(s): 門野敬子・Dorington O.Ogoyi・城所久良子・味村正博・小瀬川英一・伴野豊・前川秀彰・中島裕美子, ・行弘研司・伊藤克彦・山本公子・三田和英
  • Organizer: 日本蚕糸学会第80回記念大会.平成22年度 蚕糸・昆虫利用学術講演会
  • Place of Presentation: 長野県上田
  • Year and Date: 2010-04-04
• [Presentation] 日本産クワコのミトコンドリアCO1遺伝子の塩基多型にもとづく集団間分化の検討 (2010)
  • Author(s): 行弘研司、岩田和也、冨田秀一郎、河本夏雄、門野敬子、伊藤雅信、伴野豊、木内信
  • Organizer: 日本蚕糸学会第80回記念大会.平成22年度 蚕糸・昆虫利用学術講演会
  • Place of Presentation: 長野県上田
  • Year and Date: 2010-04-04
• [Presentation] カイコ・クワコのBmTNMLローカスにおける、転移因子の挿入と切り出しイベントの年代推定 (2010)
  • Author(s): 中島裕美子・川西祐一・前川秀彰・行弘研司・間野修平
  • Organizer: 日本蚕糸学会第80回記念大会.平成22年度 蚕糸・昆虫利用学術講演会
  • Place of Presentation: 長野県上田
  • Year and Date: 2010-04-03
• [Presentation] Estimation of the periods some different transposable elements insert into and move off from the host genome in the BmTNML Locus of Bombyx mori and Bombyx mandarina (2010)
  • Author(s): Mano, S., Kawanishi, Y., Maekawa, H., Nakajima, Y.
  • Organizer: 4th Annual Arthropod Genomics Symposium
  • Place of Presentation: Kansas City, MO USA
• [Presentation] Estimation of the time some transposons insert into and move out from the host genome at the BmTNML locus of Bombyx mori and Bombyx mandarina (2010)
  • Author(s): Mano, S., Yukuhiro, K., Kawanishi, Y., Maekawa, H., Nakajima, Y.
  • Organizer: 6th International Conference on wild silkmoths
  • Place of Presentation: Tokyo University of Agriculture, Tokyo
• [Presentation] カイコ・クワコのBmTNML locusでの転移因子の挿入と切り出しイベントの年代推定 (2010)
  • Author(s): 川西祐一、間野修平、行弘研司、前川秀彰、中島裕美子
  • Organizer: 第33回日本分子生物学会・第83回日本生化学会合同大会
  • Place of Presentation: 神戸国際会議場、神戸
• [Presentation] Bombyx familyの進化 (2010)
  • Author(s): 味村、前川、中島、伴野、阿部、行弘、瀬筒、門野、下村、大門、矢後、A.Promboon, R.Urs, S.Gu, Q.Xia, J Nagaraju, 嶋田、三田
  • Organizer: 平成22年度日本蚕糸学会合同支部大会
  • Place of Presentation: 浦添てだこホール、沖縄
• [Presentation] Silkwor genome annotation for comparative genomics and functional genomics (2010)
  • Author(s): K.Mita, R.Futahashi, Y.Suetsugu, K.Kadono-Okuda, 他8名
  • Organizer: 6th International Conference on wild silkmoths
  • Place of Presentation: Tokyo University of Agriculture, Tokyo(招待講演)
• [Presentation] MOLECULAR GENETICS OF THE DENSOVIRUS RESISTANCE GENES IN BOMBYX SILKWORMS (2010)
  • Author(s): K.Kadono-Okuda, D.O.Ogoyi, K.Kidokoro, M.Ajimura, E.Kosegawa, Y.Banno, H.Maekawa, Y.Nakajima, K.Yukuhiro, K.Ito, K.Yamamoto, K.Mita
  • Organizer: 6th International Conference on wild silkmoths
  • Place of Presentation: Tokyo University of Agriculture, Tokyo
• [Presentation] DNA polymorphism in Japanese populations of wild mulberry silkmoth Bombyx mandarina (2010)
  • Author(s): K.Yukuhiro, K.Iwata, N.Komoto, S.Tomita, K.Kadono-Okuda, M.Kiuchi, M.Ito, Y.Banno
  • Organizer: 6th International Conference on wild silkmoths
  • Place of Presentation: Tokyo University of Agriculture, Tokyo
• [Presentation] Evolution of Bombyx family (2010)
  • Author(s): M.Ajimura, H.Maekawa, Y.Nakajima, Y.Banno, H.Abe, K.Yukuhiro, H.Sezutsu, K.Kadono-Okuda, T.Daimon, A.Promboon, R.Urs, S.Gu, Q.Xia, J.Nagaraju, T.Shimada, K.Mita
  • Organizer: 6th International Conference on wild silkmoths
  • Place of Presentation: Tokyo University of Agriculture, Tokyo
• [Presentation] Silkworm genome annotation for comparative genomics and functional genomics (2010)
  • Author(s): K.Mita, 他11名
  • Organizer: 6th International Conference on wild silkmoths
  • Place of Presentation: Tokyo University of Agriculture, Tokyo(招待講演)
• [Presentation] Molecular genetics of densovirus resistance in the silkworm, Bombyx mori (2009)
  • Author(s): K.Kadono-Okuda, D.O.Ogoyi, K.Kidokoro, M.Ajimura, E.Kosegawa, Y.Banno, H.Maekawa, Y.Nakajima, K.Yukuhiro, K.Ito, K.Yamamoto, K.Mita
  • Organizer: International Symposium on Bombyx mori Functional Genomics and Modern Silk Road
  • Place of Presentation: 中国重慶
  • Year and Date: 2009-10-23
• [Presentation] カイコ濃核病ウイルス抵抗性の分子遺伝学 (2009)
  • Author(s): 門野敬子, Dorington O.Ogoyi, 城所久良子, 味村正博, 小瀬川英一, 伴野豊, 前川秀彰, 中島裕美子, 行弘研司, 伊藤克彦, 山本公子, 三田和英
  • Organizer: 日本遺伝学会大会ワークショップ
  • Place of Presentation: 長野県上田
  • Year and Date: 2009-09-18
• [Presentation] The application of the MLE sequences as the "Migration Trace Indicator" among the different population of the same species. (2009)
  • Author(s): NAKAJIMA Yumiko, KAWANISHI Yuichi, MANO Shuhei, MAEKAWA Hideaki
  • Organizer: International Symposium of Marine Genomics, December 15-18, Okinawa, Japan
  • Place of Presentation: Okinawa, Japan
• [Presentation] The analysis of the specific mariner Transposon in some insects, BmTNML locus and the R2 retrotransposable elements of B.mandarina compared with those of B.mori. (2009)
  • Author(s): Y.Kawanishi, Y.Kamauchi, S.Okuma, S.Mano, Y.Banno, S.K.Nho, Yu-Feng Hsu, K.Ueda, M.Yago, K.Odagiri, T.H.Eickbush, H.Maekawa, Y.Nakajima
  • Organizer: The 6th Asia-Pacific Congress of Entomology, October 18-22, Beijing, 2009, China
  • Place of Presentation: Beijing, China
• [Book] Molecular Biology and Genetics of the Lepidoptera (Densovirus Resistance in Bombyx mori) (2010)
  • Author(s): Kadono-Okuda Keiko(分担)
  • Total Pages: 362
  • Publisher: CRC Press
• [Book] Molecular Biology and Genetics of the Lepidoptera (Sex Chromosomes and Sex Determination in Bombyx mori) (2010)
  • Author(s): Abe Hiroaki, Fujii Tsuguru, Shimada Toru(分担)
  • Total Pages: 362
  • Publisher: CRC Press
• [Book] 分子昆虫学 ポストゲノムの昆虫研究 (1.5突然変異遺伝子の単離) (2009)
  • Author(s): 門野敬子(分担)
  • Total Pages: 426
  • Publisher: 共立出版株式会社