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Molecular analysis of novel factors involved in repression of infection and propagation of a DNA virus in silkworm.

Research Project

Project/Area Number 21380043
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied entomology
Research InstitutionNational Institute of Agrobiological Sciences

Principal Investigator

TANAKA Hiromitsu  独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, 主任研究員 (30391577)

Co-Investigator(Kenkyū-buntansha) SEZUTSU Hideki  独立行政法人農業生物資源研究所, 遺伝子組換え研究開発ユニット, ユニット長 (70342805)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
Keywords昆虫病理 / カイコ / 核多角体病ウイルス / Etsファミリー遺伝子 / Tollタンパク質 / immediate early遺伝子 / 遺伝子ノックダウン / Etsファミリータンパク質 / Toll
Research Abstract

RNA interference and overexpression experiments using silkworm culture cells demonstrated that an Ets family transcription factor, BmEts was involved in repression of propagation of BmNPV, which belongs to DNA viruses, in the silkworm cells. BmEts suppressed the activation of promoter activity of viral immediate early gene. Furthermore, the expression of BmEts gene was shown to be up-regulated in response to bacterial challenge. BmEts enhanced the expression of a silkworm antimicrobial peptide gene, lebocin. On the other hand, BmToll10-3was shown to be involved in the promoter activation of viral immediate early gene, suggesting that BmToll10-3induces activation of transcription factors for enhancement of the immediate early gene promoter activity, in contrast to BmEts. We believe that these findings contribute to elucidation of the mechanisms of insect immunity against DNA viruses.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (27 results)

All 2012 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 7 results) Presentation (20 results)

  • [Journal Article] BmEts upregulates promoter activity of lebocin in2012

    • Author(s)
      Tanaka H, Sagisaka A, Fujita K, Furukawa S, Ishibashi J, Yamakawa M
    • Journal Title

      Bombyx mori Insect Biochemistry and Molecular Biology

      Volume: 42(7) Pages: 474-481

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] BmEts upregulates promoter activity of lebocin in Bombyx mori2012

    • Author(s)
      Tanaka H, Sagisaka A, Fujita K, Furukawa S, Ishibashi J, Yamakawa M
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: (In press) Issue: 7 Pages: 474-481

    • DOI

      10.1016/j.ibmb.2012.03.004

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effective RNA interference in cultured silkworm cells mediated by over expression of Caenorhabditis elegans SID-12012

    • Author(s)
      Mon H, Kobayashi I, Ohkubo S, Tomita S, Lee JM, Sezutsu H, Tamura T, Kusakabe T
    • Journal Title

      RNA Biol

      Volume: 9 Issue: 1 Pages: 1-8

    • DOI

      10.4161/rna.9.1.18084

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] SID-1 protein of Caenorhabditis elegans mediates uptake of dsRNA into Bombyx cells2012

    • Author(s)
      Kobayashi I, Tsukioka H, Komoto N, Uchino K, Sezutsu H, Tamura T, Kusakabe T, Tomita S
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 42 Issue: 2 Pages: 148-154

    • DOI

      10.1016/j.ibmb.2011.11.007

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of the innate immune responses in the silkworm, Bombyx mori2011

    • Author(s)
      Tanaka H, Yamakawa M
    • Journal Title

      Invertebrate Survival Journal

      Volume: 8 Pages: 59-69

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression profiling of novel bacteria-induced genes from the silkworm2010

    • Author(s)
      Tanaka H, Suzuki N, Nakajima Y, Sato M, Sagisaka A, Fujita K, Ishibashi J, Imanishi S, Mita K, Yamakawa M
    • Journal Title

      Bombyx mori Archives of Insect Biochemistry and Physiology

      Volume: 73(3) Pages: 148-162

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression profiling of novel bacteria-induced genes from the silkworm, Bombyx mori2010

    • Author(s)
      田中博光
    • Journal Title

      Archives of Insect Biochemistry and Physiology 73

      Pages: 148-162

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] カイコEtsファミリータンパク質の自然免疫への関与2011

    • Author(s)
      田中博光,勾坂晶,石橋純,山川稔
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      神奈川県横浜市パシフィコ横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Final Research Report
  • [Presentation] カイコEtsファミリータンパク質の自然免疫への関与2011

    • Author(s)
      田中博光, 勾坂晶, 石橋純, 山川稔
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] 培養細胞を用いたカイコのウイルス感染応答遺伝子、BmEtsの機能解析2011

    • Author(s)
      勾坂晶,石橋純,山川稔,田中博光
    • Organizer
      平成23年度日本蚕糸学会合同支部大会昆虫機能・利用学術講演会第65回東北支部第62回関東支部第77回関西支部第67回九州支部合同大会
    • Place of Presentation
      岩手県盛岡市岩手大学農学部
    • Year and Date
      2011-11-05
    • Related Report
      2011 Final Research Report
  • [Presentation] 培養細胞を用いたカイコのウイルス感染応答遺伝子、BmEtsの機能解析2011

    • Author(s)
      勾坂晶, 石橋純, 山川稔, 田中博光
    • Organizer
      平成23年度日本蚕糸学会合同支部大会
    • Place of Presentation
      岩手大学農学部
    • Year and Date
      2011-11-04
    • Related Report
      2011 Annual Research Report
  • [Presentation] Etsファミリータンパク質のカイコ生体防御機構への関与2011

    • Author(s)
      田中博光
    • Organizer
      昆虫ワークショップ2011東北
    • Place of Presentation
      山形県山形市ZAOセンタープラザ
    • Year and Date
      2011-10-14
    • Related Report
      2011 Final Research Report
  • [Presentation] Etsファミリータンパク質のカイコ生体防御機構への関与2011

    • Author(s)
      田中博光
    • Organizer
      昆虫ワークショップ2011東北
    • Place of Presentation
      ZAOセンタープラザ
    • Year and Date
      2011-10-14
    • Related Report
      2011 Annual Research Report
  • [Presentation] BmEtsの抗微生物ペプチド遺伝子プロモーターの活性制御解析2011

    • Author(s)
      田中博光、勾坂晶、石橋純、山川稔
    • Organizer
      平成23年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      講演要旨集の発行のみ
    • Year and Date
      2011-03-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] カイコ培養細胞におけるBmNPV感染応答遺伝子の機能解析2011

    • Author(s)
      勾坂晶、石橋純、山川稔、田中博光
    • Organizer
      平成23年度蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      講演要旨集の発行のみ
    • Year and Date
      2011-03-21
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of transgenic silkworm bombyx mori for functional genomics2011

    • Author(s)
      Sezutsu H, Uchino K, Tsubota T, Osanai-Futahashi M, Suzuki T, Tatematsu K I, Tamura T
    • Organizer
      Sixth International Symposium on Molecular Insect Science
    • Place of Presentation
      Amsterdam, The Netherland
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of transgenic silkworms for functional genomics in Bombyx mori as a lepidopteran model insect 5th Annual Arthropod Genomics Symposium2011

    • Author(s)
      Sezutsu H, Tsubota T, Uchino K, Kobayashi I, Osanai-Futahashi M, Suzuki T, Tatematsu K I, Iizuka T, Yonemura N, Tamura T
    • Organizer
      5th Annual Arthropod Genomics Symposium
    • Place of Presentation
      Kansas, USA
    • Related Report
      2011 Annual Research Report
  • [Presentation] BmEtsはウイルス由来遺伝子の遺伝子発現に抑制的に作用する2010

    • Author(s)
      勾坂晶、田中博光、藤田幸輔、石橋純、山川稔
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] BmEtsはウイルス由来遺伝子の遺伝子発現に抑制的に作用する2010

    • Author(s)
      勾坂晶,田中博光,藤田幸輔,石橋純,山川稔
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      兵庫県神戸市神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] Microarray analysis of host gene expression in the silkworm cells infected with Bombyx mori nucleopolyhedrovirus2010

    • Author(s)
      勾坂晶、石橋純、今西重雄、三田和英、山川稔、田中博光
    • Organizer
      Current Opinion in Cellular Host-Pathogen Interactions
    • Place of Presentation
      オランダ アムステルダム市
    • Year and Date
      2010-09-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Microarray analysis of host gene expression in the silkworm cells infected with Bombyx mori nucleopolyhedrovirus2010

    • Author(s)
      Sagisaka A, Ishibashi J, Imanishi S, Mita K, Yamakawa M, Tanaka H
    • Organizer
      Current Opinion in Cellular Host-Pathogen Interactions
    • Place of Presentation
      オランダアムステルダム
    • Year and Date
      2010-09-07
    • Related Report
      2011 Final Research Report
  • [Presentation] BmNPV感染応答遺伝子であるBmEtsの機能解析2010

    • Author(s)
      勾坂晶,田中博光,石橋純,山川稔
    • Organizer
      平成22年度蚕糸・昆虫機能利用学術講演会日本蚕糸学会第80回記念大会
    • Place of Presentation
      長野県上田市信州大学繊維学部
    • Year and Date
      2010-04-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 細菌応答遺伝子として同定されたカイコEtsファミリータンパク質遺伝子の発現解析及び遺伝子産物の機能解析2010

    • Author(s)
      田中博光,勾坂晶,石橋純,山川稔
    • Organizer
      平成22年度蚕糸・昆虫機能利用学術講演会日本蚕糸学会第80回記念大会
    • Place of Presentation
      長野県上田市信州大学繊維学部
    • Year and Date
      2010-04-04
    • Related Report
      2011 Final Research Report
  • [Presentation] カイコゲノム情報から明らかになったカイコの自然免疫2009

    • Author(s)
      田中博光
    • Organizer
      日本蚕糸学会関東支部第60回大会蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      栃木県宇都宮市宇都宮大学農学部
    • Year and Date
      2009-11-07
    • Related Report
      2011 Final Research Report
  • [Presentation] カイコゲノム情報から明らかになったカイコの自然免疫2009

    • Author(s)
      田中博光
    • Organizer
      日本蚕糸学会関東支部第60回大会 蚕糸・昆虫機能利用学術講演会
    • Place of Presentation
      宇都宮大学 農学部
    • Year and Date
      2009-11-07
    • Related Report
      2009 Annual Research Report
  • [Presentation] RNAi法を用いてのカイコ核多角体病ウイルスの感染・感染防御に関わるカイコ由来因子の探索2009

    • Author(s)
      田中博光,勾坂晶,藤田幸輔,石橋純,今西重雄,三田和英,山川稔
    • Organizer
      同定昆虫ポストゲノム研究会
    • Place of Presentation
      北海道札幌市北海道大学農学部
    • Year and Date
      2009-09-11
    • Related Report
      2011 Final Research Report
  • [Presentation] RNAi法を用いてのカイコ核多角体病ウイルスの感染・感染防御に関わるカイコ由来因子の探索・同定2009

    • Author(s)
      田中博光
    • Organizer
      昆虫ポストゲノム研究会
    • Place of Presentation
      北海道大学 農学部
    • Year and Date
      2009-09-11
    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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