Molecular analysis of novel factors involved in repression of infection and propagation of a DNA virus in silkworm.
| Project/Area Number |
21380043
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
TANAKA Hiromitsu 独立行政法人農業生物資源研究所, 昆虫機能研究開発ユニット, 主任研究員 (30391577)
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| Co-Investigator(Kenkyū-buntansha) |
SEZUTSU Hideki 独立行政法人農業生物資源研究所, 遺伝子組換え研究開発ユニット, ユニット長 (70342805)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 昆虫病理 / カイコ / 核多角体病ウイルス / Etsファミリー遺伝子 / Tollタンパク質 / immediate early遺伝子 / 遺伝子ノックダウン / Etsファミリータンパク質 / Toll |
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| Research Abstract |
RNA interference and overexpression experiments using silkworm culture cells demonstrated that an Ets family transcription factor, BmEts was involved in repression of propagation of BmNPV, which belongs to DNA viruses, in the silkworm cells. BmEts suppressed the activation of promoter activity of viral immediate early gene. Furthermore, the expression of BmEts gene was shown to be up-regulated in response to bacterial challenge. BmEts enhanced the expression of a silkworm antimicrobial peptide gene, lebocin. On the other hand, BmToll10-3was shown to be involved in the promoter activation of viral immediate early gene, suggesting that BmToll10-3induces activation of transcription factors for enhancement of the immediate early gene promoter activity, in contrast to BmEts. We believe that these findings contribute to elucidation of the mechanisms of insect immunity against DNA viruses.
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Report
(4 results)
Research Products
(27 results)
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[Presentation] Development of transgenic silkworms for functional genomics in Bombyx mori as a lepidopteran model insect 5th Annual Arthropod Genomics Symposium2011
Author(s)
Sezutsu H, Tsubota T, Uchino K, Kobayashi I, Osanai-Futahashi M, Suzuki T, Tatematsu K I, Iizuka T, Yonemura N, Tamura T
Organizer
5th Annual Arthropod Genomics Symposium
Place of Presentation
Kansas, USA
Related Report
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