Study on the control of gene expression network in Streptomyces growing in the soil environment

• Project/Area Number: 21380050
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant nutrition/Soil science
• Research Institution: National Institute for Agro-Environmental Sciences
• Principal Investigator: FUJII Takeshi 独立行政法人農業環境技術研究所, その他部局等, 研究領域長 (00354100)
• Co-Investigator(Kenkyū-buntansha): SAITO Akihiro 静岡理工科大学, 理工学部, 准教授 (50375614)
• Project Period (FY): 2009-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000); Fiscal Year 2012: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2009: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
• Keywords: 土壌生物 / 遺伝子発現 / マイクロアレイ解析 / 放線菌 / キチナーゼ / 土壌環境 / 抗生物質生産 / キチン / 遺伝子発現制御 / 細菌 / マイクロアレイ / 酵素 / 抗生物質 / 発現制御

Research Abstract

To provide new insights into the cellular response to nutrients and regulation cascade of antibiotic production genes in global gene expression of Streptomyces in soil, genome-wide transcriptional analysis with microarray technology was performed using RNA extracted from the wild type and mutant strains of Streptomyces coelicolor grown in soil cultures. Microarray analyses revealed that expression of genes for secondary metabolism was induced by chitin together with primary metabolic genes in soil cultures of Streptomyces coelicolor. The data also indicated that a transcriptional factor, DasR, was involved in the regulation of gene expression for chitin catabolism, secondary metabolism and stress responses.

Research Products

• [Journal Article] Chitin-induced gene expression in secondary metabolic pathways of Streptomyces coelicolor A3(2) grown in soil (2013)
  • Author(s): Nazari, B., Kobayashi, M., Saito, A., Hassaninasab, A., Miyashita, K., Fujii, T.
  • Journal Title: Appl. Environ. Microbiol Volume: 79 Issue: 2 Pages: 707-713
  • DOI: 10.1128/aem.02217-12
  • Peer Reviewed
• [Journal Article] Enzymatic and genetic characterization of the DasD protein possessing N-acetyl-β-D- glucosaminidase activity in Streptomyces coelicolor A3(2) (2013)
  • Author(s): Saito, A., Ebise, H., Orihara, Y., Murakami, S., Sano, Y., Sugiyama, Y., Ando, A., Fujii, T., Miyashita, K.
  • Journal Title: F EMS Microbiol. Lett Volume: 340 Issue: 1 Pages: 33-40
  • DOI: 10.1111/1574-6968.12069
  • Peer Reviewed
• [Journal Article] 土壌からのRNAの抽出技術とその利用技術の開発 (2013)
  • Author(s): 藤井毅, 王勇
  • Journal Title: 土壌微生物相の解明による土壌微生物性の解析技術の開発(農林水産省研究成果) Volume: 494 Pages: 25-28
• [Journal Article] これまで困難だった黒ボク土壌からのRNA抽出法の開発 (2013)
  • Author(s): 王勇, 長岡和成, 早津雅仁, 酒井順子, 多胡香奈子, 藤井毅
  • Journal Title: 農業環境技術研究所研究成果情報 Volume: 29 Pages: 36-37
• [Journal Article] 土壌環境下、放線菌の抗生物質生産遺伝子群の発現がキチンによって誘導される (2013)
  • Author(s): ベナムナザリ, 藤井毅
  • Journal Title: 農業環境技術研究所研究成果情報 Volume: 29 Pages: 38-39
• [Journal Article] A novel method for RNA extraction from Andosols using casein and its application to amoA gene expression study in soil (2012)
  • Author(s): Wang, Y., Nagaoka, K., Hayatsu M., Sakai Y., Tago K., Asakawa S., Fujii, T.
  • Journal Title: Appl. Microbiol. Biotechnol Volume: 96(3) Issue: 3 Pages: 793-802
  • DOI: 10.1007/s00253-012-4342-3
  • Peer Reviewed
• [Journal Article] Extraction of Bacterial RNA from Soil: Challenges and Solutions (2012)
  • Author(s): Wang, Y., Hayatsu, M., Fujii, T.
  • Journal Title: Microbes and Environments Volume: 27 Issue: 2 Pages: 111-121
  • DOI: 10.1264/jsme2.ME11304
  • NAID: 10030431536
  • ISSN: 1342-6311, 1347-4405
  • Peer Reviewed
• [Journal Article] Specific gene responses of Rhodococcus jostii RHA1 during growth in soil (2012)
  • Author(s): Iino, T., Wang, Y., Miyauchi, K., Kasai, D., Masai, E., Fujii, T., Ogawa, N., Fukuda M.
  • Journal Title: Appl. Environ. Microbiol Volume: 78(19) Issue: 19 Pages: 6954-6962
  • DOI: 10.1128/aem.00164-12
  • Peer Reviewed
• [Journal Article] Classification of Chitosanases by Hydrolytic Specificity toward <i>N</i>¹,<i>N</i>⁴-Diacetylchitohexaose (2012)
  • Author(s): Hirano K, Watanabe M, Seki K, Ando A, Saito A, Mitsutomi M
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 10 Pages: 1932-1937
  • DOI: 10.1271/bbb.120408
  • NAID: 10031126483
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Identification of potato scab-causing Streptomyces sp. occurring in strongly acidic soils in Saga Prefecture in Japan (2012)
  • Author(s): Tashiro N, Manabe K, Saito A, Miyashita K
  • Journal Title: J. Gen. Plant Pathol Volume: 78 Issue: 5 Pages: 353-359
  • DOI: 10.1007/s10327-012-0393-7
  • Peer Reviewed
• [Journal Article] Expression of a bacterial chitosanase in rice plants improves disease resistance to the rice blast fungus Magnaporthe oryzae (2012)
  • Author(s): Kouzai Y, Mochizuki S, Saito A, Ando A, Minami E, Nishizawa Y
  • Journal Title: Plant Cell Rep Volume: 31 Issue: 4 Pages: 629-636
  • DOI: 10.1007/s00299-011-1179-7
  • Peer Reviewed
• [Journal Article] <i>Aleuria aurantia</i> Lectin Exhibits Antifungal Activity against <i>Mucor racemosus</i> (2012)
  • Author(s): Amano K, Katayama H, Saito A, Nagata Y
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Issue: 5 Pages: 967-970
  • DOI: 10.1271/bbb.110982
  • NAID: 10030752918
  • ISSN: 0916-8451, 1347-6947
  • Peer Reviewed
• [Journal Article] Classification of chitosanase by hydrolytic specificities toward N1,N4-diacetylchitohexaose (2012)
  • Author(s): Hirano K, Watanabe M, Seki K, Ando A, Saito A, Mitsutomi M
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 76 Pages: 1932-1937
  • NAID: 10031126483
  • Peer Reviewed
• [Journal Article] A survey of the cellular responses in Pseudomonas putida KT2440 growing in sterilized soil by microarray analysis (2011)
  • Author(s): Wang, Y., Morimoto, S., Ogawa, N., Fujii, T.
  • Journal Title: F EMS Microbiol Ecol Volume: 78 Issue: 3 Pages: 220-232
  • DOI: 10.1111/j.1574-6941.2011.01143.x
  • Peer Reviewed
• [Journal Article] High expression levels of chitinase genes in Streptomyces coelicolor A3(2) grown in soil (2011)
  • Author(s): Nazari, B., Kobayashi, M., Miyashita, K., Saito, A., Wang, Y., Fujii, T
  • Journal Title: F EMS Microbiol. Ecol Volume: 77 Pages: 623-635
  • Peer Reviewed
• [Journal Article] Molecular and enzymatic characterization of a subfamily I.4 lipase from an edible oil degrader Bacillus sp. HH-01 (2011)
  • Author(s): Kamijo, T., Saito, A., Ema, S., You, I., Hayashi, H., Nagata, R., Nagata, Y., Ando, A.
  • Journal Title: Anton. Leeuw. Int. J . G Volume: 99 Issue: 2 Pages: 179-187
  • DOI: 10.1007/s10482-010-9474-9
  • Peer Reviewed
• [Journal Article] Streptomyces coavervatus sp. nov., isolated from the intestinal tract of Armadillidium vulgae (2011)
  • Author(s): Shibasaki, A., Kudo, T., Yaguchi, T., Saito, A., Ando, A., Mikami, Y., Gonoi, T.
  • Journal Title: Int. J . Syst. Enviol. Micr Volume: 61(ijs.0.019091-0v161/5/1073) Pages: 1073-1077
  • Peer Reviewed
• [Journal Article] Biological control of Rhizoctonia damping-off of cucumber by a transformed Pseudomonas putida strain expressing a chitinase from a marine bacterium (2011)
  • Author(s): Ohno, M., Kataoka, S., Numata, S., Yamamoto-Tamura, K., Fujii, T., Nakajima, M., Akutsu, K., Hasebe, A.
  • Journal Title: J ARQ-J pn. Agr. Res. Q Volume: 45(1) Pages: 91-98
  • NAID: 130002147920
  • URL: http://www.jircas.affrc.jp
  • Peer Reviewed
• [Journal Article] Assessment of the effects of genetically modified Pseudomonas spp. expressing chitinase on the soil microbial community in the cucumber rhizosphere (2011)
  • Author(s): Yamamoto-Tamura, K., Ohno M., Fujii T., Kataoka, S., Numata, S., Nakajima, M., Hasebe A., Akutsu K.
  • Journal Title: JARQ-Jpn. Agr. Res. Q Volume: 45(4) Pages: 377-383
  • NAID: 130002147951
  • URL: http://www.jircas.affrc.jp
  • Peer Reviewed
• [Journal Article] メタゲノムからの新規遺伝子探索 (2011)
  • Author(s): 森本晶, 藤井毅
  • Journal Title: 土と微生物 Volume: 65 Pages: 66-72
  • Peer Reviewed
• [Journal Article] スギ落葉から分離したPestalotiopsis sp.属糸状菌が生産するカテコール配糖化酵素の精製と諸性質 (2011)
  • Author(s): 齋藤政則, 篠山浩文, 齋藤明広, 知久和寛, 安藤昭一
  • Journal Title: 食と緑の科学 Volume: 65 Pages: 75-80
  • Peer Reviewed
• [Journal Article] Evaluation of Methods of Determining Humic Acids in Nucleic Acid Samples for Molecular Biological Analysis (2011)
  • Author(s): Wang, Y., Fujii, T
  • Journal Title: Bioscience, Biotechnology, and Biochemistry Volume: 75 Issue: 2 Pages: 355-357
  • DOI: 10.1271/bbb.100597
  • NAID: 10027898077
  • ISSN: 0916-8451, 1347-6947
• [Journal Article] A survey of the cellular responses in Pseudomonas putida KT2440 growing in sterilized soil by microarray analysis (2011)
  • Author(s): Wang, Y., Morimoto, S., Ogawa, N., Fujii, T.
  • Journal Title: FEMS Microbiology Ecology Volume: 78 Issue: 2 Pages: 220-232
  • DOI: 10.1111/j.1574-6941.2011.01146.x
  • Peer Reviewed
• [Journal Article] Streptomyces coavervatus sp.nov., isolated from the intestinal tract of Armadillidium vulgae (2011)
  • Author(s): Shibasaki, A., Kudo, T., Yaguchi, T., Saito, A., Ando, A., Mikami, Y., Gonoi, T.
  • Journal Title: International Journal of Systematic and Environmental Microbiology Volume: 61 Pages: 1073-1077
  • Peer Reviewed
• [Journal Article] Biological control of Rhizoctonia damping-off of cucumber by a transformed Pseudomonas putida strain expressing a chitinase from a marine bacterium (2011)
  • Author(s): Ohno, M., Kataoka, S., Numata, S., Yamamoto-Tamura, K., Fujii, T., Nakajima, M., Akutsu, K., Hasebe, A.
  • Journal Title: Agricultural Research Quarterly Volume: 45 Pages: 91-98
  • NAID: 130002147920
  • Peer Reviewed
• [Journal Article] Assessment of the effects of genetically modified Pseudomonas spp.expressing chitinase on the soil microbial community in the cucumber rhizosphere (2011)
  • Author(s): Yamamoto-Tamura, K., Ohno M., Fujii T., Kataoka, S., Numata, S., Nakajima, M., Hasebe A., Akutsu K.
  • Journal Title: Agricultural Research Quarterly Volume: 45 Pages: 377-383
  • Peer Reviewed
• [Journal Article] スギ落葉から分離したPestalotiopsis sp.属糸状菌が生産するカテコール配糖化酵素の精製と諸性質.食と緑の科学.65:75-80 (2011)
  • Author(s): 齋藤政則, 篠山浩文, 齋藤明広, 知久和寛, 安藤昭一
  • Journal Title: 食と緑の科学 Volume: 65 Pages: 75-80
  • Peer Reviewed
• [Journal Article] Evaluation of Methods for Determining Humic Acids in the Nucleic Acids Samples for Molecular Biological Analysis. (2011)
  • Author(s): Wang, Y., Fujii, T.
  • Journal Title: Biosci.Biotechnol.Biochem. Volume: 75(2) Pages: 355-357
  • Peer Reviewed
• [Journal Article] An improved method to extract RNA form soil with efficient removal of humic acids (2009)
  • Author(s): Wang, Y., Morimoto, S., Ogawa, N., Ohmori, T., Fujii, T.
  • Journal Title: J . Appl. Microbiol Volume: 107 Issue: 4 Pages: 1168-1177
  • DOI: 10.1111/j.1365-2672.2009.04298.x
  • Peer Reviewed
• [Journal Article] 農耕地土壌におけるメタゲノム解析研究の動向
  • Author(s): 藤井毅
  • Journal Title: 化学と生物 Volume: 49 Pages: 697-703
  • NAID: 10029758050
• [Presentation] N-アセチル-β-D-グルコサミニダーゼDasDの放線菌Streptomyces coelicolor A3(2)における機能の解析 (2013)
  • Author(s): 齋藤明広, 村上聡史, 海老瀬広規, 杉山裕多, 安藤昭一, 藤井毅, 宮下清貴
  • Organizer: 日本農芸化学会2013年度大会
  • Place of Presentation: 仙台・東北大学
  • Year and Date: 2013-03-26
• [Presentation] 土壌抽出RNA から明らかになった抗生物質生産遺伝子の土壌における誘導発現 (2013)
  • Author(s): 藤井毅
  • Organizer: 農業環境技術研究所30 周年記念セミナー公開セミナー
  • Place of Presentation: 秋葉原コンベンションホール
  • Year and Date: 2013-03-07
• [Presentation] 細胞質局在型N-アセチル-β-D-グルコサミニダーゼDasDの放線菌Streptomyces coelicolor A3(2)における機能の解析 (2013)
  • Author(s): 齋藤明広，村上聡史，海老瀬広規，杉山裕多，安藤昭一，藤井毅，宮下清貴
  • Organizer: 日本農芸化学会
  • Place of Presentation: 東北大学
• [Presentation] The role of DasR in regulation of chitin metabolism of Streptomyces coelicolor grown in soil (2012)
  • Author(s): Nazari,B., Kobayashi, M., Fujii, T.
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-23
• [Presentation] The role of DasR in regulation of chitin metabolism of Streptomyces coelicolor grown in soil (2012)
  • Author(s): Behnam Nazari, Michihiko Kobayashi, Takeshi Fujii
  • Organizer: 日本農芸化学会(日本農芸化学会2011年度大会)
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 2012-03-23
• [Presentation] Microarray Analysis of the Transcriptional Responses of Streptomyces coelicolor to the Addition of Chitin into Soil Microcosm (2012)
  • Author(s): Nazari,B., Kobayashi, M., Fujii, T.
  • Organizer: International Conference Molecular Ecology
  • Place of Presentation: University of Veterinary Medicine, オーストリア
  • Year and Date: 2012-02-06
• [Presentation] Microarray Analysis of the Transcriptional Responses of Streptomyces coelicolor to the Addition of Chitin into Soil Microcosm (2012)
  • Author(s): Behnam Nazari, Michihiko Kobayashi, Takeshi Fujii
  • Organizer: International Conference Molecular Ecology
  • Place of Presentation: University of Veterinary Medicine(ウイーン)
  • Year and Date: 2012-02-06
• [Presentation] Sequence analysis of a fosmid clone possessing AOA amoA and 16S rDNA from agricultural soil (2012)
  • Author(s): Sakai Y, Hayatsu M, Fujii T
  • Organizer: 日本微生物生態学会
  • Place of Presentation: 豊橋技術科学大学
• [Presentation] A universal method for RNA extraction from soil using casein and its application to amoA gene expression study in Andosols (2012)
  • Author(s): Wang Y, Nagaoka K, Hayatsu M, Sakai Y, Tago K, Asakawa S, Akiyama H, Fujii T
  • Organizer: 日本分子生物学会
  • Place of Presentation: 福岡国際会議場・マリンメッセ福岡
• [Presentation] Biodegradation of chitin in soil by Streptomyces coelicolor A3(2) (2012)
  • Author(s): Nazari B, Kobayashi M, Fujii T
  • Organizer: 日本放線菌学会
  • Place of Presentation: 府中の森芸術劇場
• [Presentation] Microarray analysis revealed a highly responsive gene cluster to 3-chlorobenzoic acid in Pseudomonas putida KT2440 growing in sterilized soil (2011)
  • Author(s): Wang, Y., Morimoto, S., Ogawa, N., Fujii T. and, Hayatsu, M.
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] analysis revealed a highly responsive gene cluster to 3-chlorobenzoic acid in Pseudomonas putida KT2440 growing in sterilized soil (2011)
  • Author(s): Yong Wang, Sho Morimoto, Naoto Ogawa, Takeshi Fujii, Masahiyo Hayatsu
  • Organizer: 日本分子生物学会(第34回日本分子生物学会年会)
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2011-12-14
• [Presentation] Genome-wide transcriptomic analysis of Streptomyces coelicolor grown in soil in response to the addition of chitin (2011)
  • Author(s): Nazari,B., Kobayashi, M., Fujii, T.
  • Organizer: 日本農芸化学会関東支部2011年度大会
  • Place of Presentation: 東洋大学
  • Year and Date: 2011-10-15
• [Presentation] Genome-wide transcriptomic analysis of Streptomyces coelicolor grown in soil in response to the addition of chitin (2011)
  • Author(s): Behnam Nazari, Michihiko Kobayashi, Takeshi Fujii
  • Organizer: 日本農芸化学会(日本農芸化学会関東支部2011年度大会)
  • Place of Presentation: 東洋大学・板倉キャンパス(群馬県板倉町)
  • Year and Date: 2011-10-15
• [Presentation] Microbial gene expression analysis in soil using microarray technology (2011)
  • Author(s): Wang, Y., Nagaoka, K., Morimoto, S., Ogawa, N., Hayatsu, M., and Fujii T.
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学
  • Year and Date: 2011-10-09
• [Presentation] Microbial gene expression analysis in soil using microarray technology (2011)
  • Author(s): Yong Wang, Kazunari Nagaoka, Sho Morimoto, Naoto Ogawa, Masahiyo Hayatsu, Takeshi Fujii
  • Organizer: 日本微生物生態学会(第27回日本微生物生態学会大会)
  • Place of Presentation: 京都大学(京都市)
  • Year and Date: 2011-10-09
• [Presentation] The unique chitinase gene expressions in Streptomyces coelicolor grown in soil (2011)
  • Author(s): Nazari,B., Kobayashi, M., Fujii, T.
  • Organizer: 第84回日本生化学大会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-09-23
• [Presentation] The unique chitinase gene expressions in Streptomyces coelicolor grown in soil (2011)
  • Author(s): Nazari Behnam, Michihiko Kobayashi, Takeshi Fujii
  • Organizer: 日本生化学会(第84回日本生化学大会)
  • Place of Presentation: 国立京都国際会館(京都市)
  • Year and Date: 2011-09-23
• [Presentation] 放線菌Streptomyces coelicolor A3(2)のDasDタンパク質は細胞内局在型β-N-アセチルグルコサミニダーゼとして機能する (2011)
  • Author(s): 折原由香里, 海老瀬広規, 村上聡史, 齋藤明広, 安藤昭一, 藤井毅, 宮下清貴
  • Organizer: 日本キチン・キトサン学会(第25回キチン・キトサンシンポジウム)
  • Place of Presentation: 奈良県新公会堂(奈良市)
  • Year and Date: 2011-08-31
• [Presentation] キチン2糖輸送系に変異を持つ放線菌を用いたキチン2糖生産の検討 (2011)
  • Author(s): 太箸勇太, 齋藤明広
  • Organizer: 日本キチン・キトサン学会(第25回キチン・キトサンシンポジウム)
  • Place of Presentation: 奈良県新公会堂(奈良市)
  • Year and Date: 2011-08-31
• [Presentation] 放線菌Streptomyces coelicolor A3(2)のDasDタンパク質は細胞内局在型β-N-アセチルグルコサミニダーゼとして機能する (2011)
  • Author(s): 折原由香里, 海老瀬広規, 村上聡史, 齋藤明広, 安藤昭一, 藤井毅, 宮下淸貴
  • Organizer: 第25回キチン・キトサンシンポジウム
  • Place of Presentation: 奈良県新公会堂
  • Year and Date: 2011-08-30
• [Presentation] 放線菌Streptomyces coelicolor A3(2)におけるキトサナーゼ遺伝子の探索 (2011)
  • Author(s): 茂木麻衣, 海老瀬広規, 村上聡史, 佐野ユカリ, 石井健雄, 木村明音, 齋藤明広, 安藤昭一
  • Organizer: 日本キチン・キトサン学会(第25回キチン・キトサンシンポジウム)
  • Place of Presentation: 奈良県新公会堂(奈良市)
  • Year and Date: 2011-08-30
• [Presentation] 放線菌Streptomyces coelicolor A3(2)のdasD遺伝子のキチン分解における機能の解明 (2011)
  • Author(s): 海老瀬広規, 折原由香里, 村上聡史, 齋藤明広, 安藤昭一, 藤井毅, 宮下清貴
  • Organizer: 日本土壌肥料学会(2011年度つくば大会)
  • Place of Presentation: エポカルつくば(つくば市)
  • Year and Date: 2011-08-09
• [Presentation] 放線菌Streptomyces coelicolor A3(2)におけるMsiK-ABC輸送系複合体の探索 (2011)
  • Author(s): 海老瀬広規, 村上聡史, 佐藤守, 安藤昭一, 齋藤明広
  • Organizer: 第8回大腸菌研究会
  • Place of Presentation: ホテル木曽路
  • Year and Date: 2011-05-18
• [Presentation] 放線菌Streptomyces coelicolor A3(2)におけるMsiK-ABC輸送系複合体の探索 (2011)
  • Author(s): 海老瀬広規, 村上聡史, 佐藤守, 安藤昭一, 齋藤明広
  • Organizer: 第8回大腸菌研究会(第8回大腸菌研究会)
  • Place of Presentation: ホテル木曽路(長野県木曽町)
  • Year and Date: 2011-05-18
• [Presentation] 放線菌Streptomyces coelicolor A3(2)のdasD遺伝子のキチン分解における機能の解明 (2011)
  • Author(s): 海老瀬広規, 折原由香里, 村上聡史, 齋藤明広, 安藤昭一, 藤井毅, 宮下清貴
  • Organizer: 日本土壌肥料学会2011年度大会
• [Presentation] Soil specific chitinase gene expression in Streptomyces coelicolor A3(2) (2010)
  • Author(s): Nazari,B., Kobayashi, M., Fujii, T.
  • Organizer: International Symposium on New Frontiers in Microbiology and Biotechnology
  • Place of Presentation: 中国,江南大学
  • Year and Date: 2010-09-29
• [Presentation] Soil specific chitinase gene expression in Streptomyces coelicolor A3(2) (2010)
  • Author(s): Behnam, N., Kobayashi, M., Fujii, T.
  • Organizer: International Symposium on New Frontiers in Microbiology and Biotechnology
  • Place of Presentation: 中国、江南大学
  • Year and Date: 2010-09-29
• [Presentation] Chitinase production and N,N'- diacetylchitobiose uptake in Streptomyces coelicolor A3(2) (2010)
  • Author(s): Saito, A., Fujii, T., Shinya, T., Shibuya, N., Ando, A., Miyashita, K.
  • Organizer: The 11th International Conference on Chitin and Chitosan Symposium
  • Place of Presentation: 台湾, 台湾理工科大学
  • Year and Date: 2010-09-08
• [Presentation] Chitinase-production and N,N'-diacetylchitobiose-uptake in Strepomyces coelicolor A3(2) (2010)
  • Author(s): Saito, A., Fujii, T., Shinya, T.Shibuya, N., Ando, A., Miyashita, K.
  • Organizer: The 11^<th> International Conference on Chitin and Chitosan and The 8^<th> Asia-Pacific Chitin and Chitosan Symposium
  • Place of Presentation: 台湾、台湾理工科大学
  • Year and Date: 2010-09-08
• [Presentation] Purification and characterization of a soil specific chitinase from Streptomyces coelicolor A3(2) (2010)
  • Author(s): Behnam, N., Kobayashi, M., Fujii, T.
  • Organizer: 日本放線菌学会
  • Place of Presentation: 東京、船堀
  • Year and Date: 2010-09-02
• [Presentation] Detection of genome-wide gene expression in Pseudomonas putida KT-2440 growing in soil by microarray analysis (2010)
  • Author(s): Wang, Y., Morimoto, S., Ogawa, N. and Fujii, T.
  • Organizer: ISME 13
  • Place of Presentation: 米国, シアトル
  • Year and Date: 2010-08-27
• [Presentation] Detection of genome-wide gene expression in Pseudomonas putida KT-2440 growing in soil by microarray analysis (2010)
  • Author(s): Wang, Y., Morimoto, S., Ogawa, N., Fujii, T.
  • Organizer: International Symposium on Microbial Ecology 13
  • Place of Presentation: 米国、シアトル
  • Year and Date: 2010-08-27
• [Presentation] Purification and characterization of a soil specific chitinase from Streptomyces coelicolor A3(2) (2010)
  • Author(s): Nazari,B., Kobayashi, M., and Fujii, T.
  • Organizer: 日本放線菌学会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-14
• [Presentation] 放線菌Streptomyces coelicolor A3(2)における2つの(GlcNAc)_2輸送系の役割 (2010)
  • Author(s): 齋藤明広, 飯沼千晴, 新屋友規, 出崎能丈, 澁谷直人, 安藤昭一, 藤井毅, 宮下清貴
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京、東京大学
  • Year and Date: 2010-07-14
• [Presentation] 放線菌Streptomyces coelicolor A3(2)のキチナーゼ生産におけるDasRの機能解析 (2010)
  • Author(s): 佐野ユカリ, 村上聡史, 海老瀬広規, 齋藤明広, 新屋友規, 出﨑能丈, 澁谷直人, 安藤昭一, 藤井毅, 宮下清貴
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2010-07-13
• [Presentation] 放線菌Streptomyces coelicolor A3(2)における2つの(GlcNAc)2輸送系の役割 (2010)
  • Author(s): 齋藤明広, 飯沼千晴, 新屋友規, 出﨑能丈, 澁谷直人, 安藤昭一, 藤井毅, 宮下清貴
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京大学
  • Year and Date: 2010-07-13
• [Presentation] 放線菌Streptomyces coelicolor A3(2)のキチナーゼ生産におけるDasRの機能解析 (2010)
  • Author(s): 佐野ユカリ, 村上聡史, 海老瀬広規, 齋藤明広, 新屋友規, 出崎能丈, 澁谷直人, 安藤昭一, 藤井毅, 宮下清貴
  • Organizer: 第24回キチン・キトサンシンポジウム
  • Place of Presentation: 東京、東京大学
  • Year and Date: 2010-07-13
• [Presentation] Detection of soil specific gene expression of a chitinase in Streptomyces coelicolor (2010)
  • Author(s): Nazari,B., Kobayashi, M., Fujii, T.
  • Organizer: 日本土壌微生物学会
  • Place of Presentation: 東京大学
  • Year and Date: 2010-05-21
• [Presentation] Detection of soil specific gene expression of a chitinase in Streptomyces coelicolor (2010)
  • Author(s): Behnam, N., Kobayashi, M.Fujii, T.
  • Organizer: 日本土壌微生物学会
  • Place of Presentation: 東京、東京大学
  • Year and Date: 2010-05-21
• [Presentation] From soil to transcriptome-the soil RNA-centered study (2010)
  • Author(s): Yong Wang, Sho Morimot, Naoto Ogawa, Takeshi Fujii
  • Organizer: 第4回日本ゲノム微生物学会
  • Place of Presentation: 九州大学医学部百年講堂
  • Year and Date: 2010-03-07
• [Presentation] Chitinase-production and N, N'-diacetylchitobiose-uptake in Streptomyces coelicolor A3(2) (2009)
  • Author(s): Saito A, Fujii T, Shinya T, Shibuya N, Ando A, Miyashita K
  • Organizer: The 11^<th> International Conference on Chitin and Chitosan and The 8^<th> Asia-Pacific Chitin and Chitosan Symposium
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2009-09-07
• [Presentation] 放線菌でのキトサン分解産物の輸送とキトサナーゼ遺伝子の発現制御 (2009)
  • Author(s): 木村明音, 齋藤明広, 安藤昭一
  • Organizer: 日本キチン・キトサン学会
  • Place of Presentation: 佐賀
  • Year and Date: 2009-08-20
• [Presentation] An improved method to extract RNA from soil with efficient removal of humic acids (2009)
  • Author(s): Wang, Y., Morimoto, S., Ogawa, N., Fujii T.
  • Organizer: BGECO 10
  • Place of Presentation: スウエーデン・ウプサラ
  • Year and Date: 2009-06-15
• [Presentation] An improved method to extract RNA from soil with efficient removal of humic acids (2009)
  • Author(s): Yong Wang, Sho Morimoto, Naoto Ogawa, Takeshi Fujii
  • Organizer: BAGECO10(Bacterial Genetics and Ecology-Coexisting on a Changing Planet)
  • Place of Presentation: スウエーデン・ウプサラ
  • Year and Date: 2009-06-15
• [Book] Application of PCR-DGGE and metagenome walking to retrieve full-lengths functional genes from soil. In Molecular Microbial Ecology II(Ed. Bruijn, F. J. de) (2011)
  • Author(s): Morimoto, S., Fujii, T
  • Publisher: Wiley-Blackwell, Hoboken, New Jersey
• [Book] Molecular Microbial Ecology II(一部執筆担当)"Application of PCR-DGGE and metagenome walking to retrieve full-lengths functional genes from soil"(P.117～123) (2011)
  • Author(s): Sho Morimoto, Takeshi Fujii
  • Total Pages: 7
  • Publisher: Wiley-Blackwell, Hoboken, New Jersey
• [Book] 食品衛生学実験書」第2章第5節「遺伝子検査」(P.60～62)(中村好志・白尾美佳編著) (2011)
  • Author(s): 齋藤明広
  • Total Pages: 3
  • Publisher: 光生館
• [Book] 物質と生命の科学(惣田〓夫・出口潔編著) (2011)
  • Author(s): 齋藤明広
  • Total Pages: 195
  • Publisher: 現代図書
• [Book] 農耕地土壌の生物学的特性解明への挑戦, メタゲノム解析技術の最前線 (2010)
  • Author(s): 藤井毅
  • Publisher: シーエムー出版
• [Book] メタゲノム解析技術の最前線(共著) (2010)
  • Author(s): 藤井毅
  • Total Pages: 231
  • Publisher: シーエム出版
• [Remarks]
  • URL: http://www.niaes.affrc.go.jp/researcher/fujii_t.html
• [Remarks]
  • URL: http://www.sist.ac.jp/col/teacher/search/detail/655
• [Remarks]
  • URL: http://www.niaes.affrc.go.jp/researcher/fujii_t.html