Mechanisms for eukaryotic response and adaptation to hypoxia

• Project/Area Number: 21380055
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: University of Tsukuba
• Principal Investigator: TAKAYA Naoki 筑波大学, 生命環境系, 教授 (50282322)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2009: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
• Keywords: 真菌 / 嫌気呼吸 / 低酸素応答 / エネルギー代謝 / オミクス / カビ / 低酸素 / 発酵 / 酸化還元 / 代謝調節 / ストレス応答 / 醗酵

Research Abstract

Oxygen depletion imposes a challenge on fungi since oxygen serves as a substrate for respiration and for other biological processes. Our investigation using omics and biochemical approaches revealed novel adaptation mechanisms to such environment of the Aspergillus fungi. These mechanisms are considered be important for the fungal adaptation into natural environment as well as into artificial fermenting conditions.

Research Products

• [Journal Article] Hydrolase controls cellular nicotinamide adenine dinucleotide, sirtuin and secondary metabolites (2012)
  • Author(s): Shimizu T.
  • Journal Title: Mol. Cell. Biol. Volume: 32 Issue: 18 Pages: 3743-3755
  • DOI: 10.1128/mcb.00032-12
  • Peer Reviewed
• [Journal Article] Heme-biosynthetic porphobilinogen deaminase protects Aspergillus nidulans from nitrosative stress (2012)
  • Author(s): Zhou, S., Narukami, T., Nameki, M., Ozawa, T., Kamimura, Y., Hoshino, T., and Takaya, N
  • Journal Title: Appl. Environ. Microbiol. Volume: 78 Issue: 1 Pages: 103-109
  • DOI: 10.1128/aem.06195-11
  • Peer Reviewed
• [Journal Article] Conserved and specific responses to hypoxia in Aspergillus oryzae and Aspergillus nidulans determined by comparative transcriptomics (2012)
  • Author(s): Terabayashi, Y., Shimizu, M., Kitazume, T., Masuo, S., Fujii, T., Takaya, N.
  • Journal Title: Appl.Microbiol.Biotechnol Volume: 93 Issue: 1 Pages: 307-315
  • DOI: 10.1007/s00253-011-3767-4
  • Peer Reviewed
• [Journal Article] Glutathione reductase/glutathione is responsible for cytotoxic elemental sulfur tolerance via polysulfide shuttle in fungi (2011)
  • Author(s): Sato, I., Shimatani, K., Fujita, K., Abe, T., Shimizu, M., Fujii, T., Hoshino, T., and Takaya, N
  • Journal Title: J. Biol. Chem. Volume: 286 Pages: 20283-20291
  • Peer Reviewed
• [Journal Article] Glutathione reductase/glutathione is responsible for cytotoxic elemental sulfur tolerance via polysulfide shuttle in fungi (2011)
  • Author(s): Sato I.
  • Journal Title: J. Biol. Chem Volume: 286 Issue: 23 Pages: 20283-20291
  • DOI: 10.1074/jbc.m111.225979
  • Peer Reviewed
• [Journal Article] Mechanism of de novo branched-chain amino acid synthesis as an alternative electron sink in hypoxic Aspergillus nidulans cells. (2010)
  • Author(s): Shimizu, M., Fujii, T., Masuo, S., and Takaya, N
  • Journal Title: Appl. Environ. Microbiol. Volume: 76 Pages: 1507-1515
  • Peer Reviewed
• [Journal Article] Global gene expression analysis of Aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia (2010)
  • Author(s): Masuo, S., Terabayashi, Y., Shimizu, M., Fujii, T., Kitazume, T., and Takaya, N
  • Journal Title: and transcription suppression under hypoxia. Mol. Genet. Genomics. Volume: 284 Pages: 415-424
  • Peer Reviewed
• [Journal Article] Multi-energy metabolic mechanisms of the fungus Fusarium oxysporum under lower oxygen environments. (2010)
  • Author(s): Zhou, Z., Takaya, N., and Shoun, H
  • Journal Title: Biosci. Biotechnol. Biochem. Volume: 74 Pages: 2431-2437
  • Peer Reviewed
• [Journal Article] Global gene expression analysis of Aspergillus nidulans reveals metabolic shift and transcription suppression under hypoxia (2010)
  • Author(s): Masuo, S., Terabayashi, Y., Shimizu, M., Fujii, T., Kitazume, T, Takaya, N.
  • Journal Title: Mol.Gen.Genet. Volume: 284 Pages: 1415-4241
  • Peer Reviewed
• [Journal Article] Mechanism of de novo branched-chain amino acid synthesis as alternative electron sink in hypoxic Aspergillus nidulans cells. (2010)
  • Author(s): Shimizu, M., Fujii, T., Masuo, S., Takaya, N.
  • Journal Title: Appl.Environ.Microbiol. Volume: 76 Pages: 1507-1515
  • Peer Reviewed
• [Journal Article] The glutathione system of Aspergillus nidulans involves a fungus-specific glutathione S-transferase. (2009)
  • Author(s): Sato, I., Shimizu, M., Hoshino, T., and Takaya, N
  • Journal Title: J. Biol. Chem. Volume: 284 Pages: 8042-8053
  • Peer Reviewed
• [Journal Article] Proteomic analysis of Aspergillus nidulans cultured under hypoxic conditions. (2009)
  • Author(s): Shimizu, M., Fujii, T., Masuo, S., Fujita, K., and Takaya, N
  • Journal Title: Proteomics Volume: 8 Pages: 7-19
  • Peer Reviewed
• [Journal Article] The glutathione system of Aspergillus nidulans involves a fungal specific glutathione-S-transferase (2009)
  • Author(s): Sato, I., Shimizu, M., Hoshino, T., Takaya, N.
  • Journal Title: J.Biol.Chem. Volume: 284 Pages: 8042-8053
  • Peer Reviewed
• [Presentation] Aspergillus nidulansの元素状硫黄還元反応に関わる酵素の機能解析 (2010)
  • Author(s): 島谷佳奈果、佐藤育男、高谷直樹
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-05
• [Presentation] Aspergillus nidulans のメナジオンストレス下におけるNmrAの機能 (2010)
  • Author(s): 桝尾俊介、志水元亨、高谷直樹
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-05
• [Presentation] Roles of Aspergillus nidulans proteins in thioredoxin reductase superfamily (2009)
  • Author(s): Sato, I., Shimiatani, K., Shimizu, M., Takaya, N
  • Organizer: Microbial Interactions Leading to Novel Biological Functions
  • Place of Presentation: Tsukuba
  • Year and Date: 2009-11-20
• [Remarks]
  • URL: http://www.microbes.agbi.tsukuba.ac.jp/takaya/