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Analyses of regulation of respiration by bacterial signals

Research Project

Project/Area Number 21380056
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied microbiology
Research InstitutionUniversity of Tsukuba

Principal Investigator

NOMURA Nobuhiko  筑波大学, 生命環境系, 准教授 (60292520)

Co-Investigator(Kenkyū-buntansha) UCHIYAMA Hiroo  筑波大学, 生命環境系, 教授 (00185042)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2009: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Keywords呼吸 / バイオフィルム / 細胞間コミュニケーション / 硝化 / 活性汚泥 / 細菌 / 細菌シグナル / エネルギー代謝 / 微生物複合系
Research Abstract

Regulation of bacterial growth is generally studied in relation to physicochemical conditions ; however, how a bacterial community regulate itself remains obscure. Recently, it was demonstrated that cell-to-cell communication molecules regulate respiration in Pseudomoans aeruginosa. To gain more insight into how growth is regulated in the presence of other bacterial species, the effect of a P. aeruginosa produced cell-to-cell communication molecule on the growth of other bacteria was studied. In conclusion, bacterial signals seem to be a multifunctional molecule affecting other bacterial species ; furthermore, this cell-to-cell communication molecule may influence the bacterial community development by regulating bacterial growth, for which physicochemical factors are important.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (27 results)

All 2012 2011 2010 2009 Other

All Journal Article (19 results) (of which Peer Reviewed: 19 results) Presentation (6 results) Book (1 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Social behaviours under anaerobic conditions in Pseudomonas aeruginosa2012

    • Author(s)
      Masanori Toyofuku, Hiroo Uchiyama, Nobuhiko Nomura
    • Journal Title

      International Journal of Microbiology

      Volume: (印刷中) Pages: 1-7

    • DOI

      10.1155/2012/405191

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multifunctional membrane vesicles in Pseudomonas aeruginosa2012

    • Author(s)
      Yosuke Tashiro, Hiroo Uchiyama, Nobuhiko Nomura
    • Journal Title

      Environmental Microbiology

      Volume: (印刷中) Issue: 6 Pages: 1349-1362

    • DOI

      10.1111/j.1462-2920.2011.02632.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Continuous monitoring of ammonia removal activity and observation of morphology of microbial complexes in a microdevice2011

    • Author(s)
      Toda K, Yawata Y, Setoyama E, Fukuda J, Nomura N, Suzuki H
    • Journal Title

      Applied Environmental Microbiology

      Volume: 77(12) Pages: 4253-5

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of phospholipids in membrane vesicles derived from Pseudomonas aeruginosa2011

    • Author(s)
      Tashiro Y, Inagaki A, Shimizu M, Ichikawa S, Takaya N, Nakajima-Kambe T, Uchiyama H, Nomura N*
    • Journal Title

      Biosci Biotechnol Biochem

      Volume: 75(3) Pages: 605-7

    • NAID

      10028202208

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Continuous monitoring of ammonia removal activity and observation of morphology of microbial complexes in a microdevice2011

    • Author(s)
      Toda K., et al.
    • Journal Title

      Applied Environmental Microbiology

      Volume: 77 Issue: 12 Pages: 4253-5

    • DOI

      10.1128/aem.01246-10

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Variation of physiochemical properties and cell association activity of membrane vesicles with growth phase in Pseudomonas aeruginosa2010

    • Author(s)
      Tashiro Y, S Ichikawa, N Nomura*
    • Journal Title

      Applied Environmental Microbiology

      Volume: 76(11) Pages: 3732-9

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring Biofilm Growth in a Microfludic Device by a Modified Confocal Reflection Microscopy Method2010

    • Author(s)
      Yawata Y, K Toda, H Suzuki, N Nomura
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 110(3) Pages: 377-80

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visualizing the Effects of Biofilm Structures on the Influx of Fluorescent Material Using Combined Confocal Reflection and Fluorescent Microscopy2010

    • Author(s)
      Yawata Y, H Uchiyama, N Nomura
    • Journal Title

      Microbes and Environments

      Volume: 25 Pages: 49-52

    • NAID

      10025921677

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] The Effect of a Cell-to-Cell Communication Molecule, Pseudomonas Quinolone Signal (PQS), Produced by P. aeruginosa on Other Bacterial Species2010

    • Author(s)
      Toyofuku M, T Nakajima-Kambe, H Uchiyama, N Nomura
    • Journal Title

      Microbes and Environments

      Volume: 25 Pages: 1-7

    • NAID

      10025921415

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Bicy clic compounds repress membrane vesicle production and Pseudomonas quinolone signal synthesis in Pseudomonas aeruginosa2010

    • Author(s)
      Tashiro Y, M Toyofuku, N Nomura
    • Journal Title

      FEMS Microbiology Letters

      Volume: 304 Pages: 123-130

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] バイオフィルム研究技術の新展開2010

    • Author(s)
      八幡穣, 戸田憲輔, 瀬戸山恵里香, 福田淳二, 鈴木博章, 稲葉知大, 内山裕夫, 野村暢彦
    • Journal Title

      環境バイオテクノロジー学会誌

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Variation of physiochemical properties and cell association activity of membrane vesicles with growth phase in Pseudomonas aeruginosa2010

    • Author(s)
      Tashiro Y, et al.
    • Journal Title

      Applied Environmental Microbiology

      Volume: 76 Pages: 3732-3739

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bicy clic compounds repress membrane vesicle production and Pseudom on as quinolone signal synthesis in Pseudomonas aemginosa.2010

    • Author(s)
      Tashiro Y, et al.
    • Journal Title

      FEMS Microbiology Letters

      Volume: 304 Pages: 123-130

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pseudomonas quinolone signal affects membrane vesicle production in not only Gram negative but also Gram-positive bacteria2010

    • Author(s)
      Tashiro Y, et al.
    • Journal Title

      Microbes and Environments

      Volume: 25 Pages: 120-125

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Effect of a Cell-to-Cell Communication Molecule, Pseudomonas Quinolone Signal (PQS), Produced by P.aeruginosa on Other Bacterial Species2010

    • Author(s)
      Toyofuku M, et al.
    • Journal Title

      Microbes & Environments

      Volume: Vol 25 Pages: 1-7

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Outer membrane machinery and alginate synthesis regulators control membrane vesicle production in Pseudomonas aeruginosa2009

    • Author(s)
      Tashiro Y, R Sakai, N Namura
    • Journal Title

      Journal of Bacteriology

      Volume: 191 Pages: 7509-7519

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Outer membrane machinery and alginate synthesis regulators control membrane vesicle production in Pseudomonas aeruginosa.2009

    • Author(s)
      Tashiro Y, et al.
    • Journal Title

      Journal of Bacteriology

      Volume: Vol 191 Pages: 7509-7519

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Multifunctional membrane vesicles in Pseudomonas aeruginosa

    • Author(s)
      Tashiro Y, Uchiyama H, Nomura N*
    • Journal Title

      Environmental Microbiology (2011) in press (Review)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Social behaviours under anaerobic conditions in Pseudomonas aeruginosa

    • Author(s)
      Toyofuku M, Uchiyama H, Nomura N*
    • Journal Title

      International Journal of Microbiology (2011) in press (Review)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Presentation] バイオフィルムの基礎研究から臨床への展開へ、小林宏行先生メモリアルセミナー「病原微生物のバイオフィルムと疾病の関わり基礎と臨床展望-」2011

    • Author(s)
      野村暢彦
    • Organizer
      第60回日本感染症学会東日本地方会学術集会・第58回日本化学療法学会東日本支部総会・合同学会
    • Place of Presentation
      ホテルメトロポリタン山形
    • Year and Date
      2011-10-27
    • Related Report
      2011 Final Research Report
  • [Presentation] バイオフィルムの基礎研究臨床への展開へ2011

    • Author(s)
      野村暢彦
    • Organizer
      第60回日本感染症学会・日本化学療法学会合同大会
    • Place of Presentation
      ホテルメトロポリタン山形
    • Year and Date
      2011-10-27
    • Related Report
      2011 Annual Research Report
  • [Presentation] 「微生物集合体バイオフィルム内の細胞外DNAの役割」、細胞外核酸の動態:医科学・環境科学での新しい観点からの研究・応用展開2011

    • Author(s)
      野村暢彦
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Year and Date
      2011-09-02
    • Related Report
      2011 Final Research Report
  • [Presentation] 「マイクロデバイス技術と新規イメージング技術の融合による新しい微生物解析技術の開発-微生物のありのままの非破壊・非侵襲的かつ経時的な解析」、「ゲノム微生物学会ワークショップ-ゲノムで繋がる微生物研究の新展開-」2011

    • Author(s)
      野村暢彦
    • Organizer
      2011年度ゲノム微生物学会大会
    • Place of Presentation
      東北大学
    • Year and Date
      2011-08-20
    • Related Report
      2011 Final Research Report
  • [Presentation] 細菌のバイオフィルム形成と治療薬の開発2010

    • Author(s)
      野村暢彦
    • Organizer
      第84回日本感染症学会総会
    • Place of Presentation
      京都国際会議場
    • Year and Date
      2010-04-06
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 環境中の微生物生態解析に向けて:Meta-cellularからsub-cellularまで2009

    • Author(s)
      野村暢彦
    • Organizer
      東京大学生物生産工学研究センター シンポジウム
    • Place of Presentation
      東京大学弥生講堂一条ホール(東京都)
    • Year and Date
      2009-12-11
    • Related Report
      2009 Annual Research Report
  • [Book] バイオフィルム研究の基盤技術2009

    • Author(s)
      八幡穣, 内山裕夫, 野村暢彦
    • Publisher
      臨床と微生物近代出版36巻
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] Method for treating wastewater containing ammonia nitrogen2012

    • Inventor(s)
      Nomura N., et al.
    • Industrial Property Rights Holder
      住友重機械工業(株)
    • Filing Date
      2012-07-02
    • Related Report
      2011 Final Research Report
    • Overseas

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Published: 2009-04-01   Modified: 2016-04-21  

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