| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2011: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2010: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2009: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Research Abstract |
The maturation mechanisms of subtilisins, Tk-subtilisin and Tk-SP, from the hyperthermophilic archaeon Thermococcus kodakarensis were analyzed. The results indicate that both proteins are activated(matured) upon autoprocessing and degradation of N-terminal propeprides, folding of Tk-subtilisin is induced upon binding of the Ca^<2+> ions to the Ca^<2+>-binding loop, Tk-SP is matured in the absence of the Ca^<2+> ions, Tk-SP requires C-terminalβ-jelly roll domain for hyperstability. It was also shown that Tk-subtilisin is useful for degradation of abnormal prion proteins.
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