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Study on the Structures, Functions and Applications of Sphingolipid-metabolizing machinery

Research Project

Project/Area Number 21380066
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied biochemistry
Research InstitutionKyushu University

Principal Investigator

ITO Makoto  九州大学, 大学院・農学研究院, 教授 (40253512)

Co-Investigator(Kenkyū-buntansha) OKINO Nozomu  九州大学, 大学院・農学研究院, 准教授 (90363324)
KAKUTA Yoshimitsu  九州大学, 大学院・農学研究院, 准教授 (00314360)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywords糖鎖生物学 / 酵素利用学 / スフィンゴ(糖)脂質 / 代謝 / 結晶構造 / 糖脂質 / 酵素 / 生体分子 / 糖鎖 / 菌類 / エンドグリコセラミダーゼ / EGCrP / 感染症 / スフィンゴ脂質 / 代謝酵素 / X線結晶構造解析 / 生物機能 / セラミダーゼ
Research Abstract

In this study, we explored the structures and functions of novel sphingolipid-metabolizing enzymes(ceramidase, sphingolipid ceramide N-deacylase, Hex1, NgaP, EGCrP1, EGALC), which had been identified and cloned in our laboratory. Interestingly, both ceramidase and sphingolipid ceramide N-deacylase are a zinc-dependent amidohydrolase. The cleavage of N-acyl linkage of ceramide by ceramidase follows a similar mechanism as observed for the zinc-dependent carboxypeptidases. Hex1 and NgaP were found to cleave the terminal

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (57 results)

All 2012 2011 2010 2009 2007 1999 Other

All Journal Article (25 results) (of which Peer Reviewed: 25 results) Presentation (28 results) Book (1 results) Remarks (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Quality control of fungus-specific glucosylceramide in Cryptococcus neoformans by endoglycoceramidase-related protein 1(EGCrP1)2012

    • Author(s)
      Ishibashi Y, Okino N(6人中4番目), Ito M(6人中6番目)
    • Journal Title

      J. Biol. Chem

      Volume: 287 Pages: 368-381

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Quality control of fungus-specific glucosylceraraide in Cryptococcus neoformans by endoglycoceramidase-related protein 1 (EGCrP1)2012

    • Author(s)
      Y.Ishibashi, et al
    • Journal Title

      Journal of Biological Chemistry

      Volume: 287 Issue: 1 Pages: 368-381

    • DOI

      10.1074/jbc.m111.311340

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Qualitative and quantitative cellular glycomics of glycosphingolipids based on rhodococcal endoglycoceramidase-assisted glycan cleavage, glycoblotting-assisted sample preparation, and matrix-assisted laser desorption ionization tandem time-of-flight mass2011

    • Author(s)
      Fujitani N, Ito M(9人中8番目)
    • Journal Title

      J. Biol. Chem

      Volume: 286 Pages: 41669-41679

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and catalytic mechanism of a novel glycosphingolipid-degradingβ-N-acetylgalactosaminidase from Paenibacillus sp2011

    • Author(s)
      Sumida T, Fujimoto K, Ito M
    • Journal Title

      TS12. J. Biol. Chem

      Volume: 286 Pages: 14065-14072

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Qualitative and quantitative cellular glycomics of glycosphingolipids based on Rhodococcal endoglycoceramidase-assisted glycan cleavage, glycoblotting-assisted sample preparation, and matrix-assisted laser desorption ionization tandem time-of-flight mass spectrometry analysis2011

    • Author(s)
      N.Fujitani, et al
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286 Pages: 41669-41769

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and catalytic mechanism of a novel glycosphingolipid-degrading β-N-acetylgalactosminidase from Paenibacillus sp. TS122011

    • Author(s)
      T.Sumida, et al
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286 Pages: 14065-14072

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lack of lacto/neolacto-glycolipids enhances the formation of glycolipid-enriched microdomains, facilitating B cell activation2010

    • Author(s)
      Togayachi A, Ito M(15人中11番)
    • Journal Title

      PNAS

      Volume: 107 Pages: 11900-11905

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression, purification, and characterization of a recombinant neutral ceramidase from Mycobacterium tuberculosis.2010

    • Author(s)
      Okino N, et al.
    • Journal Title

      Biosci.Biotechnol.Biochem.

      Volume: 74(2) Pages: 316-321

    • NAID

      10027551401

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lack of lacto/neolacto-glycolipids enhances the formation of glycolipid-enriched microdomains, facilitating B cell activation.2010

    • Author(s)
      Togayachi A, et al.
    • Journal Title

      Proc.Natl.Acad.Sci.USA

      Volume: 107(26) Pages: 11900-11905

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] β-Galactosyltransferase-5 is a lactosylceramide synthase essential for □mouse extra-embryonic development.2010

    • Author(s)
      Nishie T, et al.
    • Journal Title

      Glycobiology

      Volume: 20(10) Pages: 1311-1322

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous quantification of glucosylceramide and galactosylceramide by normal-phase HPLC using O-phatalaldehyde derivatives prepared with sphingolipid ceramide N-deacylase2009

    • Author(s)
      Zama K, Okino N(8人中7番), Ito M(8人中8番)
    • Journal Title

      Glycobiology

      Volume: 19 Pages: 767-775

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Transglycosylation-based fluorescent labeling of 6-gala series glycolipids by endogalactosylceramidase2009

    • Author(s)
      Ishibashi Y, Okino N(9人中7番), Ito M(9人中9番)
    • Journal Title

      Glycobiology

      Volume: 19 Pages: 797-807

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Zebrafish and mouseα2, 3-sialyltransferases responsible for synthesizing GM4 ganglioside2009

    • Author(s)
      Chisada S, Okino N(16人中14番目), Ito M.(16人中16番目)
    • Journal Title

      J. Biol. Chem

      Volume: 284 Pages: 30534-30546

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and crystal structural analysis of a novelβ-N-acetylhexosaminidase from Paenibacillus sp. TS12 capable of degrading glycosphingolipids2009

    • Author(s)
      Sumida T, Ishii R, Yanagisawa T, Yokoyama S, Ito M
    • Journal Title

      J. Mol. Biol.

      Volume: 392 Pages: 87-99

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical insights into the hydrolysis and synthesis of ceramide by neutral ceramidase2009

    • Author(s)
      Inoue T, Okino N(12人中2番目First equal contribution), Kakuta Y(12人中3番目), Ito M(12人中12番目)
    • Journal Title

      J. Biol. Chem

      Volume: 284 Pages: 9566-9577

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mechanical insights into the hydrolysis and synthesis of ceramide by neutral ceramidase2009

    • Author(s)
      Inoue, et al.
    • Journal Title

      Journal of Biological Chemistry 284

      Pages: 9566-9577

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Tamavidins--novel avidin-like biotin-binding proteins from the Tamogitake mushroom2009

    • Author(s)
      Takakura, et al
    • Journal Title

      FEBS J 276

      Pages: 1383-1397

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Simultaneous quantification of glucosylceramide and galactosylceramide by normal-phase HPLC using O-phtalaldehyde derivatives prepared with sphingolipid ceramide N-deacylase2009

    • Author(s)
      Zama, et al.
    • Journal Title

      Glycobiology 19

      Pages: 767-775

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Transglycosylation-based fluorescent labeling of 6-gala series glycolipids by endoglycoceramidase2009

    • Author(s)
      Ishibashi, et al.
    • Journal Title

      Glycobiology 19

      Pages: 797-807

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of a/b-galacoside a2, 3-sialyltransferase from a luminous marine bacterium, Photobacterium phosphoreum2009

    • Author(s)
      Iwatani, et al.
    • Journal Title

      FEBS Lett 583

      Pages: 2083-2087

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ca2+/calmodulin-dependent protein kinase phosphatase(CaMKP)is indispensable for normal embryogenesis in zebrafish, Danio rerio2009

    • Author(s)
      Sueyoshi, et al.
    • Journal Title

      Arch Biochem Biophys 488

      Pages: 48-59

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Characterization and application of carbohydrate-binding modules of b-1, 3-xylanase XUL4.2009

    • Author(s)
      Kiyohara, et al.
    • Journal Title

      J Biochem 140

      Pages: 369-373

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular cloning and crystal structural analysis of a novel b-N-acetylhexosaminidase from Paenibacillus sp. TS12 capable of degrading glycosphingoliids2009

    • Author(s)
      Sumida, et al.
    • Journal Title

      J Mol Biol 392

      Pages: 87-99

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Zebrafish and mouse a2, 3-sialyltransferases responsible for synthesizing GM4 ganglioside2009

    • Author(s)
      Chisada, et al.
    • Journal Title

      Journal of Biological Chemistry 284

      Pages: 30534-30546

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel fucosyl glycosphingolipid of brine shrimp that is highly sensitive to endoglycoceramidase2009

    • Author(s)
      Xu, et al.
    • Journal Title

      Glycobiology 141

      Pages: 1-7

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Endoglycoceramidase and its related protein EGCrP12011

    • Author(s)
      M.Ito
    • Organizer
      3rd Asian Communications of Glycobiology and Glycotechnoloey
    • Place of Presentation
      Shanghai, China(招待講演)
    • Year and Date
      2011-10-29
    • Related Report
      2011 Annual Research Report
  • [Presentation] Biological significance of non-lysosomal degradation of glucosylceramide2011

    • Author(s)
      M.Ito
    • Organizer
      Japan-Netherland Glycobiology Symposium
    • Place of Presentation
      Nagoya, Japan(招待講演)
    • Year and Date
      2011-10-09
    • Related Report
      2011 Annual Research Report
  • [Presentation] Lipid metabolism in thraustochytrids2011

    • Author(s)
      M.Ito
    • Organizer
      The 30th Naito Conference
    • Place of Presentation
      Sapporo, Japan(招待講演)
    • Year and Date
      2011-07-01
    • Related Report
      2011 Annual Research Report
  • [Presentation] Endoglycoceramidase and its related protein EGCrP12011

    • Author(s)
      Ito M
    • Organizer
      3rd Asian Communications of Glycobiology and Glycotechnology
    • Related Report
      2011 Final Research Report
  • [Presentation] Biological significance of non-lysososmal degradation of glucosylceramide2011

    • Author(s)
      Ito M
    • Organizer
      日蘭糖鎖科学シンポジウム
    • Place of Presentation
      名古屋大学、名古屋市
    • Related Report
      2011 Final Research Report
  • [Presentation] グルコシルセラミドの品質管理と細胞分裂に関与する2つのEGCrPパラログ2011

    • Author(s)
      伊東信、石橋洋平、伊藤友治、池田和貴、沖野望、合田初美、坂口圭史、田口良
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      国立京都国際会館
    • Related Report
      2011 Final Research Report
  • [Presentation] X-ray structure ofsphingolipid ceramide N-deacylase2011

    • Author(s)
      Okino N, Kakuta Y, Kato-Unoki Y, Sueyoshi N, Ito M
    • Organizer
      The 31st Naito Conference
    • Place of Presentation
      北海道札幌市
    • Related Report
      2011 Final Research Report
  • [Presentation] 全ての糖脂質から糖鎖を切り離す酵素の開発2010

    • Author(s)
      伊東信
    • Organizer
      第8回糖鎖科学コンソーシアムシンポジウム
    • Place of Presentation
      東京コンファレンスセンター、東京
    • Year and Date
      2010-11-30
    • Related Report
      2011 Final Research Report
  • [Presentation] 全ての糖脂質から糖鎖を切り離す酵素の開発2010

    • Author(s)
      伊東信
    • Organizer
      糖鎖科学コンソーシアムシンポジウム
    • Place of Presentation
      東京
    • Year and Date
      2010-11-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] The crystal structure of sphingolipid ceramide N-deacylase that catalyzes the cleavage and creation of an N-acyl linkage of various glycosphingolipids2010

    • Author(s)
      Okino N, et al.
    • Organizer
      International Carbohydrate Symposium (ICS)
    • Place of Presentation
      Makuhari, Chiba, Japan
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Discovery of a novel glucosylceramidase involved in the glucosylceramide metabolism in pathogenic fungi2010

    • Author(s)
      Ishibashi Y, et al.
    • Organizer
      International Carbohydrate Symposium (ICS)
    • Place of Presentation
      Makuhari, Chiba, Japan
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] Pathogenic vibrios preferentially adhere to GM3/GM4 having monounsaturated fatty acids2010

    • Author(s)
      Matsunaga N, et al.
    • Organizer
      International Carbohydrate Symposium (ICS)
    • Place of Presentation
      Makuhari, Chiba, Japan
    • Year and Date
      2010-08-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 真菌グルコシルセラミド合成の編集を担う酵素EGCrP2010

    • Author(s)
      伊東信
    • Organizer
      スフィンゴリピドテラピーカンファレンス(STC)
    • Place of Presentation
      鳥取
    • Year and Date
      2010-07-16
    • Related Report
      2010 Annual Research Report
  • [Presentation] Structure and functions of sphingolipid-metabolizing enzymes2010

    • Author(s)
      Ito M
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      Ventura, California, USA
    • Year and Date
      2010-02-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure and functions of sphingolipid-metabolizing machinery2010

    • Author(s)
      M.Ito
    • Organizer
      Gordon Research Conference
    • Place of Presentation
      Ventula, LA, USA
    • Year and Date
      2010-02-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] The Crystal structure of sphingolipid ceramide N-deacylase that catalyzes the cleavage and creation of an N-acyl linkage of various glycosphingolipids2010

    • Author(s)
      & Okino N, Kakuta Y, Kato-Unoki Y, Sueyoshi N, Ito M
    • Organizer
      25th International Carbohydrate Symposium
    • Place of Presentation
      Makuhari Messe International Convention Complex, Chiba, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Endoglycoceramidase-related protein(EGrP) is a novel glucosylceramidase that participates in glucosylceramide metabolism in pathogenic fungi2010

    • Author(s)
      Ishibashi Y, Okino N, Ito M
    • Organizer
      25th International Carbohydrate Symposium ; ICS2010
    • Place of Presentation
      Makuhari Messe International Convention Complex, Chiba, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] 真菌のGlcCer代謝を担う真菌特異的グルコシルセラミダーゼEGrP2010

    • Author(s)
      石橋洋平、沖野望、池田和貴、田口良、伊東信
    • Organizer
      第27回内藤コンファレンス生体膜ダイナミクスと脂質生物学
    • Place of Presentation
      シャトレーゼガトーキングダムサッポロ、札幌市
    • Related Report
      2011 Final Research Report
  • [Presentation] Rhodococcus erythropolisを宿主としたエンドグリコセラミダーゼIIおよびその活性化タンパク質の発現と機能の解析2010

    • Author(s)
      坂口圭史、田村具博、田中勲、沖野望、伊東信
    • Organizer
      第52回日本脂質生化学会
    • Place of Presentation
      伊香保温泉、群馬県
    • Related Report
      2011 Final Research Report
  • [Presentation] スフィンゴ脂質セラミドN-デアシラーゼ(SCDase)のX線結晶構造解析2009

    • Author(s)
      沖野望,角田佳充,鵜木(加藤)陽子,末吉紀行,伊東信
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] 病原性真菌より見出された新規グルコシルセラミド分解酵素2009

    • Author(s)
      石橋洋平,沖野望,伊東信
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸ポートアイランド、神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] ゼブラフィッシュを用いた糖脂質研究のインパクト2009

    • Author(s)
      伊東信、座間宏太、苣田慎一、吉村征浩、松永尚之、清水耕平、沖野望、上村聡、郷信二、池田和樹、田口良、井ノ口仁一、平林義雄
    • Organizer
      第29回日本糖質学会年会
    • Place of Presentation
      高山
    • Related Report
      2011 Final Research Report
  • [Presentation] 立体構造モデルに基づくエンドグリコセラミダーゼの特異性の解析と改変2009

    • Author(s)
      石橋洋平、小林宇太郎、坂口圭史、土方敦司、沖野望、伊東信
    • Organizer
      第29回日本糖質学会年会
    • Place of Presentation
      高山
    • Related Report
      2011 Final Research Report
  • [Presentation] 接合菌Rhizopus oryzaeより発見された新規グルコシルセラミド分解酵素2009

    • Author(s)
      石橋洋平, 他
    • Organizer
      平成21年度日本生化学会九州支部例会
    • Place of Presentation
      福岡 九州大学大学馬出キャンパス
    • Related Report
      2009 Annual Research Report
  • [Presentation] ラビリンチュラ由来のリゾリン脂質アシル転移酵素の解析2009

    • Author(s)
      安部英理子, 他
    • Organizer
      平成21年度日本生化学会九州支部例会
    • Place of Presentation
      福岡 九州大学大学馬出キャンパス
    • Related Report
      2009 Annual Research Report
  • [Presentation] セラミド代謝の生物学的インパクト2009

    • Author(s)
      伊東信
    • Organizer
      第4回スフィンゴテラピィ研究会
    • Place of Presentation
      鳥取 大山ロイヤルホテル
    • Related Report
      2009 Annual Research Report
  • [Presentation] Transglycosylation-based Fluorescent Labeling of Glycolipids by EGALC2009

    • Author(s)
      Ishibashi
    • Organizer
      50th International Conference on the Bioscience of Lipids
    • Place of Presentation
      Regensburg、Germany
    • Related Report
      2009 Annual Research Report
  • [Presentation] Transglycosylation-based fluorescent labeling of glycolipids by EGALC2007

    • Author(s)
      Ishibashi Y, Nagamatsu Y, Meyer S, Okino N, Geyer G, Ito M
    • Organizer
      50th International Conference on the Bioscience of Lipids
    • Place of Presentation
      Regensburg、Germany
    • Related Report
      2011 Final Research Report
  • [Book] セタミド:基礎戸応用(セラミドの代謝)

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/kaishika/institute/2010insti.html

    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 新規エンドグリコセラミダーゼ1999

    • Inventor(s)
      伊東信、石橋洋平、沖野望
    • Industrial Property Rights Holder
      国立大学法人九州大学
    • Acquisition Date
      1999-08-14
    • Related Report
      2011 Final Research Report
  • [Patent(Industrial Property Rights)] 新規タンパク質1999

    • Inventor(s)
      大森.、伊東信
    • Industrial Property Rights Holder
      三菱化学株式会社
    • Acquisition Date
      1999-10-09
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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