Study on the Structures, Functions and Applications of Sphingolipid-metabolizing machinery
Project/Area Number |
21380066
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied biochemistry
|
Research Institution | Kyushu University |
Principal Investigator |
ITO Makoto 九州大学, 大学院・農学研究院, 教授 (40253512)
|
Co-Investigator(Kenkyū-buntansha) |
OKINO Nozomu 九州大学, 大学院・農学研究院, 准教授 (90363324)
KAKUTA Yoshimitsu 九州大学, 大学院・農学研究院, 准教授 (00314360)
|
Project Period (FY) |
2009 – 2011
|
Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
|
Keywords | 糖鎖生物学 / 酵素利用学 / スフィンゴ(糖)脂質 / 代謝 / 結晶構造 / 糖脂質 / 酵素 / 生体分子 / 糖鎖 / 菌類 / エンドグリコセラミダーゼ / EGCrP / 感染症 / スフィンゴ脂質 / 代謝酵素 / X線結晶構造解析 / 生物機能 / セラミダーゼ |
Research Abstract |
In this study, we explored the structures and functions of novel sphingolipid-metabolizing enzymes(ceramidase, sphingolipid ceramide N-deacylase, Hex1, NgaP, EGCrP1, EGALC), which had been identified and cloned in our laboratory. Interestingly, both ceramidase and sphingolipid ceramide N-deacylase are a zinc-dependent amidohydrolase. The cleavage of N-acyl linkage of ceramide by ceramidase follows a similar mechanism as observed for the zinc-dependent carboxypeptidases. Hex1 and NgaP were found to cleave the terminal
|
Report
(4 results)
Research Products
(57 results)