Oligomer forming mechanism of lectin-like oxidized LDL receptor-1(LOX-1), and its functional reconstitution.
| Project/Area Number |
21380068
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
MACHIDA Sachiko 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品バイオテクノロジー研究領域, 上席研究員 (30353981)
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| Co-Investigator(Kenkyū-buntansha) |
ISHIKAWA Yuko 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品機能研究領域, 上席研究員 (40353940)
WATANABE Jun 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品機能研究領域, 主任研究員 (10374729)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | LOX-1 / オリゴマー化 / サンドイッチELISA / ビオチン化 / 競合ELISA / 酸化LDL / 酸化LDL受容体 / LOX1 |
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| Research Abstract |
Lectin-like oxidized LDL receptor-1(LOX-1) recognizes oxidized LDL and initiates endothelial dysfunction, which is believed to be a crucial early step in atherosclerosis. Here we show how LOX-1 forms the functional unit, and succeed to reconstitute the binding ability of LOX-1.Moreover, we develop molecular device to evaluate the OxLDL formation and molecular species, which produced on OxLDL as the results of oxidative stress.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Identification of 4-hydroxy-2-nonenal-histidine adducts that serve as ligands for human lectin-like oxidized LDL receptor-12012
Author(s)
M. K. Kuramochi, Y. Shimozu, C. Wakita, M. O. Kameyama, T. Shibata, S. Matsunaga, Y. T. Ishikawa, J. Watanabe, M. Goto, Q. Xie, S. Komba, K. Uchida, S. Machida
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Journal Title
The Biochemical Journal
Volume: 442
Pages: 171-180
Related Report
Peer Reviewed
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[Journal Article] Identification of 4-hydroxy-2-nonenal-histidine adducts that serve as ligands for human lectin-like oxidized LDL receptor-12012
Author(s)
Kumano-Kuramochi, M., Shimozu, Y., Wakita, C., Ohnishi-Kameyama, M., Shibata, T., Matsunaga, S., Takano-Ishikawa, Y., Watanabe, J., Goto, M., Xie, Q., Komba, S., Uchida, K., and Machida, S.
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Journal Title
Biochem. J
Volume: 442
Issue: 1
Pages: 171-180
DOI
Related Report
Peer Reviewed
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[Journal Article] Lipid peroxidation modification of protein generates Ne-(4-oxononanoyl)lysine as a pro-inflammatory ligand2011
Author(s)
Shibata, T., Shimozu, Y., Wakita, C., Shibata, N., Kobayashi, M., Machida, S., Kato, R., Itabe, H., Zhu,X., Sayre, L. M., and Uchida, K.
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Journal Title
J. Biol. Chem
Volume: 286
Issue: 22
Pages: 19943-19957
DOI
Related Report
Peer Reviewed
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[Journal Article] Lipid peroxidation generates a body odor component trans-2-nonenal covalently bound to protein in vivo2010
Author(s)
Ishino, K., Wakita, C., Shibata, T., Toyokuni, S., Machida, S., Matsuda, S., Matsuda, T., and Uchida, K.
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Journal Title
J. Biol. Chem
Volume: 285
Issue: 20
Pages: 15302-15313
DOI
Related Report
Peer Reviewed
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