Studies on physiological roles of ER-60 by tissue-specific gene targeting analysis

• Project/Area Number: 21380081
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Kyoto University
• Principal Investigator: URADE Reiko 京都大学, 大学院・農学研究科, 教授 (90167289)
• Co-Investigator(Kenkyū-buntansha): URADE Yoshihiro 大阪バイオサイエンス研究所, 研究部長 (10201360)
• Co-Investigator(Renkei-kenkyūsha): KASHIWAGI Kahori 大阪バイオサイエンス研究所, 研究員 (10372666) ; LAZARUS Michae 大阪バイオサイエンス研究所, 研究員 (80469650)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: ER-60 / 小胞体 / 分子シャペロン / ノックアウトマウス / 小胞体ストレス / アミロイドβ / X線結晶構造解析 / アポリポプロテインB-100 / 血清トリアシルグリセロール

Research Abstract

In this project, we found that the folding efficiency of some membrane glycoproteins was impaired by liver-specific knockout of ER-60. It was demonstrated that ER stress might be easily induced in ER-60 knockout mice by long feeding of high-fat diet. We attempted to confirm the protective effects of ER-60 against toxicity of amyloid beta peptides by brain-specific ER-60 knockout. In addition, we succeeded in X-ray structural analysis of a complex with the bb' fragment of ER-60 andβ-amyloid peptide.

Research Products

• [Journal Article] Accumulation ofβ-conglycinin in soybean cotyledon through the formation of disulfide bonds betweenα'-andα-subunits (2012)
  • Author(s): Wadahama H, Iwasaki K, Matsusaki M, Nishizawa K, Ishimoto M, Arisaka F, Takagi K, Urade R.
  • Journal Title: Plant Physiol Volume: 158 Pages: 1395-1405
  • Peer Reviewed
• [Journal Article] Acute administration of fluoxetine normalizes rapid eye movement sleep abnormality but not depressive behaviors in olfactory bulbectomized rats (2012)
  • Author(s): Wang YQ, Tu ZC, Xu XY, Li R, Qu WM, Urade Y, Huang ZL.
  • Journal Title: J. Neurochem Volume: 120 Issue: 2 Pages: 314-324
  • DOI: 10.1111/j.1471-4159.2011.07558.x
  • Peer Reviewed
• [Journal Article] Accumulation of β-conglycinin in soybean cotyledon through the formation of disulfide bonds between α'- and α-subunits (2012)
  • Author(s): Wadahama H, Iwasaki K, Matsusaki M, Nishizawa K, Ishimoto M
  • Journal Title: Plant Physiol Volume: 158 Issue: 3 Pages: 1395-1405
  • DOI: 10.1104/pp.111.189621
  • Peer Reviewed
• [Journal Article] High-Quality Protein Crystal Growth of Mouse Lipocalin-Type Prostaglandin D Synthase in Microgravity (2011)
  • Author(s): Inaka K, Takahashi S, Aritake K, Tsurumura T, Furubayashi N, Yan B, Hirota E, Sano S, Sato M, Kobayashi T, Yoshimura Y, Tanaka H, Urade Y.
  • Journal Title: Cryst. Growth Des Volume: 11 Issue: 6 Pages: 2107-2111
  • DOI: 10.1021/cg101370v
  • Peer Reviewed
• [Journal Article] Arousal effect of caffeine depends on adenosine A_<2A> receptors in the shell of the nucleus accumbens (2011)
  • Author(s): Lazarus M, Shen HY, Cherasse Y, Qu WM, Huang ZL, Bass C, Winsky-Sommerer R, Semba K, Fredholm B, Boison D, Hayaishi O, Urade Y, Chen JF.
  • Journal Title: Journal of Neuroscience Volume: 31巻 Issue: 27 Pages: 10067-10075
  • DOI: 10.1523/jneurosci.6730-10.2011
  • Peer Reviewed
• [Journal Article] A novel and comprehensive mouse model of human non-alcoholic steatohepatitis with the full range of dysmetabolic and histological abnormalities induced by gold thioglucose and a high-fat diet. (2011)
  • Author(s): Ogasawara, M., Ogasawara, M., Hirose, A., Ono, M., Aritake, K., Nozaki, Y., Takahashi, M., Okamoto, N., Sakamoto, S., Iwasaki, S., Asanuma, T., Taniguchi, T., Urade, Y., Onishi, S., Saibara, T., Oben, J.A.
  • Journal Title: Liver Int. Volume: 31 Pages: 542-551
  • Peer Reviewed
• [Journal Article] A novel and comprehensive mouse model of human non-alcoholic steatohepatitis with the full range of dysmetabolic and histological abnormalities induced by gold thioglucose and a high-fat diet (2010)
  • Author(s): Ogasawara M, Ogasawara M, Hirose A, Ono M, Aritake K, Nozaki Y, Takahashi M, Okamoto N, Sakamoto S, Iwasaki S, Asanuma T, Taniguchi T, Urade Y, Onishi S, Saibara T, Oben JA
  • Journal Title: Liver Int Volume: 31 Issue: 4 Pages: 542-551
  • DOI: 10.1111/j.1478-3231.2010.02443.x
  • Peer Reviewed
• [Journal Article] Relationship between endogenous protein disulfide isomerase family proteins and gluten in macro polymer (2010)
  • Author(s): Koh A, Nishimura K, Urade R
  • Journal Title: J. Agric. Food Chem Volume: 58 Issue: 24 Pages: 12970-12975
  • DOI: 10.1021/jf103347p
  • Peer Reviewed
• [Journal Article] Relationship between endogenous protein disulfide isomerase family proteins and glutenin macropolymer (2010)
  • Author(s): Koh, A., Nishimura, K.、Urade, R
  • Journal Title: J.Agric.Food Chem. Volume: 58 Pages: 12970-12975
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs (2009)
  • Author(s): Iwasaki K, Kamauchi S, Wadahama H, Ishimoto M, Kawada T and Urade R
  • Journal Title: FEBS J. Volume: 276 Issue: 15 Pages: 4130-4141
  • DOI: 10.1111/j.1742-4658.2009.07123.x
  • Peer Reviewed
• [Journal Article] Changes in lipid metabolism by soy beta-conglycinin-derived peptides (2009)
  • Author(s): Mochizuki, Y., Maebuchi, M., Kohno, M., Hirotsuka M, Wadahama H, Moriyama T, Kawada T and Urade R
  • Journal Title: J. Agric. Food Chem Volume: 57 Issue: 4 Pages: 1473-1480
  • DOI: 10.1021/jf8031793
  • Peer Reviewed
• [Journal Article] Changes in lipid metabolism by soy □-conglycinin-derived peptides in HepG2 cells (2009)
  • Author(s): Y.Mochizuki, M.Maebuchi, M.Kohno, M.Hirotsuka, H.Wadahama, T.Moriyama, T.Kawada, R.Urade
  • Journal Title: J.Agric.Food Chem. 57 Pages: 1473-1480
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of soybean protein disulfide isomerase family proteins with nonclassic active center motifs (2009)
  • Author(s): K.Iwasaki, S.Kamauchi, H.Wadahama, M.Ishimoto, T.Kawada, R.Urade
  • Journal Title: FEBS J. 276 Pages: 4130-4141
  • Peer Reviewed
• [Journal Article] (2009)
  • Author(s): Y.Kamiya, D.Kamiya, R.Urade, T.Suzuki, K.Kato
  • Journal Title: Glycobiology Research Trends(Sophisticated modes of sugar recognition by the intracellular lectins involved in quality control of glycoproteins) Pages: 27-40
• [Presentation] ダイズ子葉細胞におけるジスルフィド結合を介したβ-コングリシニン複合体の形成 (2012)
  • Author(s): 和田濱裕之、岩崎健佑、石本政男、西澤けいと、裏出令子
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-24
• [Presentation] ダイズ子葉細胞におるジスルフィド結合を介したβ-コングリシニン複合体の形成 (2012)
  • Author(s): 和田濱裕之、岩崎健佑、石本政男、西澤けいと、裏出令子
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-24
• [Presentation] 脳におけるER-60の発現量とレプチン作用との関係 (2012)
  • Author(s): 裏出令子、和田濱裕之、有竹浩介、永田奈々恵、裏出良博
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-23
• [Presentation] 小胞体分子シャペロンER-60の基質結合ドメインとアミロイドβペプチド複合体の結晶構造解析 (2012)
  • Author(s): 伊中浩二、古林直樹、裏出良博、魚留信子、鶴村俊治、和田濱裕之、裏出令子
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2012-03-23
• [Presentation] 脳におけるER-60の発現量とレプチン作用との関係 (2012)
  • Author(s): 裏出令子、和田濱裕之、有竹浩介、永田奈々恵、裏出良博
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-23
• [Presentation] 水胞体分子シャペロンER-60の基質結合ドメインとアミロイドβペプチド複合体の結晶構造解析 (2012)
  • Author(s): 伊中浩二、古林直樹、裏出良博、魚留信子、鶴村俊治、和田濱裕之、裏出令子
  • Organizer: 2012年度日本農芸化学会大会
  • Place of Presentation: 京都女子大学(京都府)
  • Year and Date: 2012-03-23
• [Presentation] ER-60ノックアウトの肝細胞小胞体機能への影響 (2011)
  • Author(s): 高木悠希, 有竹浩介, 大石陽, 鈴木比佐子, 和田濱裕之, 河田照雄, 裏出良博, 裏出令子
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2011-03-05
• [Presentation] ER-60ノックアウトの肝細胞小胞体機能への影響 (2011)
  • Author(s): 高木悠希, 有竹浩介, 大石陽, 鈴木比佐子, 和田濱裕之, 河田照雄, 裏出良博, 裏出令子
  • Organizer: 日本農芸化学会大会
  • Place of Presentation: 京都女子大学(大震災のため発表中止となり、講演要旨発行をもって発表成立とされた)
  • Year and Date: 2011-03-05
• [Presentation] 肝臓特異的ER-60/ERp57ノックアウトの小胞体機能への影響 (2010)
  • Author(s): 高木悠希, 有竹浩介, 大石陽, 鈴木比佐子, 和田濱裕之, 河田照雄, 裏出良博, 裏出令子
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2010-12-10
• [Presentation] レプチンによる小胞体分子シャペロンの発現調節 (2009)
  • Author(s): 和田濱裕之、有竹浩介、裏出良博、河田照雄、裏出令子
  • Organizer: 日本生化学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2009-10-21
• [Presentation] Thiol oxidoreductases involved in soybean storage protein biogenesis (2009)
  • Author(s): Urade R
  • Organizer: 34th FEBS Congress
  • Place of Presentation: Prague Congress Centre Czech Republic
  • Year and Date: 2009-07-05
• [Presentation] Thiol oxidoreductases involved in soybean storage protein biogenesis (2009)
  • Author(s): R. Urade
  • Organizer: 34^<th> FEBS Congress
  • Place of Presentation: Prague Congress Centre Czech Republic
  • Year and Date: 2009-07-05
• [Book] Flour and Breads and their Fortification in Health and Disease Prevention (2012)
  • Author(s): Reiko Urade
  • Total Pages: 542
  • Publisher: Academic Press, Elsevier
• [Book] Elsevier, Fortification of bread with soy proteins to normalize serum cholesterol and triacylglycerol levels in Flour and Breads and their Fortification in Health and Disease Prevention (2011)
  • Author(s): Urade R
  • Publisher: Academic Press
• [Book] Sophisticated modes of sugar recognition by the intracellular lectins involved in quality control of glycoproteins in Glycobiology Research Trends (2009)
  • Author(s): Kamiya Y, Kamiya D, Urade R, Suzuki T and Kato K
  • Publisher: Nova Science Publishers, Inc.