Molecular mechanism of the bamboo mass flowering revealed with cloning and identification of the flowering gene homologs

• Project/Area Number: 21380089
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Forest science
• Research Institution: Utsunomiya University
• Principal Investigator: KOBAYASHI Mikio 宇都宮大学, 農学部, 教授 (80111392)
• Co-Investigator(Kenkyū-buntansha): NATSUAKI Tomohide 宇都宮大学, 農学部, 教授 (10134264)
• Co-Investigator(Renkei-kenkyūsha): FUKUHARA Toshiyuki 東京農工大学, 共生科学技術研究科, 教授 (90228924)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000); Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2009: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
• Keywords: 森林生物 / 一斉開花 / タケモザイクウイルスBaMVV / モウハイチクPhyllostachys meyeri / 花成促進遺伝子PmFT / トウオカメザサShibataea chinensis / BaMVベクター / 花成抑制遺子PmCEN / タケ類 / プロトプラスト / タケモザイクウイルス / BaMV / 完全cDNAクローン / p35SIV(19)ベクター / 花成促進遺伝子FT / 花成抑制遺伝子CEN / タケモザイクウイルスBaMV / トウオカメーザサ / PmFT / モウハイチク

Research Abstract

Expression of flowering promoting gene, FT homologs in Phyllostachys meyeri and Shibataea chinensis was analyzed with a real-time RT-PCR technique from the beginning of mass flowering through the following several years. The expression was disappeared soon after the flowering in P. meyeri, while sustained same higher level for several years in S. chinensis. Cloned FT homolog, PmFT was confirmed extopic expression in protoplasts of P. meyeri by an electroporation method. Developing a new vector for PmFT identification based on bamboo mosaic virus was tried.

Research Products

• [Journal Article] Flowering habit of two bamboos Phyllostachys meyeri and Shibataea chinensis analyzed with flowering gene expression (2012)
  • Author(s): Hisamoto, Y. and Kobayashi, M.
  • Journal Title: Plant Species Biology Volume: 27(2) Issue: 2 Pages: 109-117
  • DOI: 10.1111/j.1442-1984.2012.00369.x
  • Peer Reviewed
• [Journal Article] 日本産タケ類における推定雑種分類群の存在意義と識別法 (2011)
  • Author(s): 小林幹夫
  • Journal Title: 東北植物研究 Volume: 16巻 Pages: 1-15
• [Journal Article] Protoplast isolation from bamboo leaves (2010)
  • Author(s): Hisamoto, Y. and Kobayashi, M.
  • Journal Title: Plant Biotechnology Volume: 27 Pages: 353-358
  • NAID: 10026706110
• [Journal Article] Protoplast isolation from bamboo leaves. (2010)
  • Author(s): Yoko Hisamoto, Mikio Kobayashi
  • Journal Title: Plant Biotechnology Volume: 27 Pages: 353-358
  • NAID: 10026706110
  • Peer Reviewed
• [Journal Article] 竹は貧困と地球温暖化の救世主となり得るか? (2009)
  • Author(s): 小林幹夫・久本洋子
  • Journal Title: 竹 Volume: 110号 Pages: 22-26
• [Journal Article] Mass flowering and flower morphology of Shibataea chinensis Nakai(Poaceae : Bambusoideae)cultivated in the Fuji Bamboo Garden, Japan (2009)
  • Author(s): Yoko Hisamoto, Harutsugu Kashiwagi, Mikio Kobayashi
  • Journal Title: The Journal of Japanese Botany 84 Pages: 167-176
  • NAID: 40016731446
  • Peer Reviewed
• [Journal Article] 第4回単子葉植物の比較生物学国際交流集会/第5回イネ科植物の分類と進化に関する国際シンポジウムMonocots IV/5^<th> International Symposium on Grass Systematics and Evolutionに参加して (2009)
  • Author(s): 久本洋子・小林幹夫
  • Journal Title: Bamboo Journal 26 Pages: 65-70
• [Journal Article] 竹は貧困と地球温暖化の救世主となり得るか?第8回世界竹会議の印象 (2009)
  • Author(s): 小林幹夫・久本洋子
  • Journal Title: 竹 110 Pages: 22-26
• [Presentation] A survey of bamboo mosaic virus and its modification to express foreign genes focusing on temperate strains (2011)
  • Author(s): Hisamoto, Y., T. Natsuaki and M. Kobayashi
  • Organizer: IBC2011 XVIII International Botanical Congress第18回国際植物学会議
  • Place of Presentation: メルボルン(オーストラリア国)
  • Year and Date: 2011-07-26
• [Presentation] タケ類の葉からのプロトプラストの調整と電気穿孔法によるGFPベクターの導入 (2010)
  • Author(s): 小林幹夫・久本洋子
  • Organizer: 日本植物学会第74回大会
  • Place of Presentation: 中部大学
  • Year and Date: 2010-09-11
• [Presentation] Flowering gene expression in the life history of two mass-flowered bamboos, Phyllostachys meyeri and Shibataea chinensis (Poaceae : Bambusoideae) (2009)
  • Author(s): Y. Hisamoto and M. Kobayashi
  • Organizer: 8th World Bamboo Congress (第八回世界竹会議)
  • Place of Presentation: バンコック(タイ国)
  • Year and Date: 2009-09-17
• [Presentation] Flowering gene expression in the life history of two mass-flowered bamboos, Phyllostachys meyeri and Shibataea chinensis(Poaceae : Bambusoideae). (2009)
  • Author(s): Yoko Hisamoto, Mikio Kobayashi
  • Organizer: 8th World Bamboo Congress(第八回世界竹会議)
  • Place of Presentation: タイ国・バンコック
  • Year and Date: 2009-09-17
• [Book] 日本の固有植物 (2011)
  • Author(s): 小林幹夫(分担執筆)
  • Total Pages: 503
  • Publisher: 東海大学出版会
• [Patent(Industrial Property Rights)] タケ類の開花予測方法 (2010)
  • Inventor(s): 小林幹夫・久本洋子
  • Industrial Property Rights Holder: 国立大学法人宇都宮大学
  • Industrial Property Number: 2010-205180
  • Filing Date: 2010-09-14
• [Patent(Industrial Property Rights)] タケ類の開花予測方法 (2009)
  • Inventor(s): 小林幹夫, 久本洋子
  • Industrial Property Rights Holder: 宇都宮大学
  • Industrial Property Number: 2009-212390
  • Filing Date: 2009-09-14