| Project/Area Number |
21380100
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,550,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥4,050,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2009: ¥9,230,000 (Direct Cost: ¥7,100,000、Indirect Cost: ¥2,130,000)
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| Keywords | クロマツ / マツ材線虫病 / トランスクリプトーム / 抵抗性育種 / 遺伝子発現 / 植物 / 遺伝子 / 病理学 / 抵抗性 / ODD |
|---|
| Research Abstract |
The Japanese black pine(Pinus thunbergii) is highly susceptible to pine wilt disease caused by the pine wood nematode(PWN ; Bursaphelenchus xylophilus). To cope with this disease, researchers and tree breeders selected PWN-resistant individuals in a previous breeding program. In an attempt to understand the mechanisms of pathopoiesis and resistance, the mechanisms were clarified using LongSAGE(serial analysis of gene expression) and ODD(ordered differential display) analyses. Especially, by the global transcriptome analysis using SAGE, it is indicated that pathogenesis-related proteins, osmotin, lipoxygenase, chalcone synthase were significantly up-regulated, and eukaryotic translation initiation factor SUI1, translationally controlled tumor protein, and xyloglucan endotransglycosylase were down-regulated by the PWN inoculation in both the resistant and non-resistant pines. On the other hand, catalase, dienelactone hydrolase family protein were expressed at a higher level only in the resistant pine, pathogenesis-related proteins and leucoanthocyanidin dioxygenase were expressed only in the non-resistant pine. These differentially expressed genes are presumed to reflect some of the differences between the resistant and non-resistant pines.
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