• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Practical use of RNA vaccine at aquaculture field.

Research Project

Project/Area Number 21380112
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General fisheries
Research InstitutionHokkaido University

Principal Investigator

YOSHIMIZU Mamoru  北海道大学, 大学院・水産科学研究院, 特任教授 (40122915)

Co-Investigator(Kenkyū-buntansha) KASAI Hisae  北海道大学, 大学院・水産科学研究院, 准教授 (50399995)
Co-Investigator(Renkei-kenkyūsha) NISHIZAWA Toyohiko  北海道大学, 大学院・水産科学研究院, 准教授 (10222184)
TAKAMI Ikuo  長崎県水産試験場, 対馬水産業普及指導センター, 所長 (50506178)
OSEKO Norihisa  水産総合研究センター増養殖研究所, 魚病診断・研修センター, センター長 (30221823)
OH Myung-Joo  Nhonnam National University, Department of Aqualife Medicine, Professor
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords魚病 / ウイルス / ワクチン / ds-RNA / Poly(I:C) / インターフェロン / 免疫
Research Abstract

In this study, we developed a novel fish-immunization with a synthetic double-stranded RNA [Poly(I:C)] and live pathogenic viruses. The fishes immunized with live pathogenic virus, followed by administration of Poly(I:C), became a transient, non-specific antiviral state, and those fish survive the initial immunization with live virus, thereby mounting a specific protective immune response against the injected pathogenic virus. Strong protective immune response was observed in rainbow trout, Japanese flounder and sevenband grouper by Poly(I:C)-immunization with IHNV, VHSV and NNV, respectively. It is conceivable that Poly(I:C)-immunization will eventually be applicable to a wide range of fish species as well as other viruses.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (82 results)

All 2013 2012 2011 2010 2009

All Journal Article (35 results) (of which Peer Reviewed: 28 results) Presentation (43 results) Book (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Assessment of the sevenband grouper Epinephelus septemfasciatus with a live nervous necrosis virus (NNV) vaccine at natural seawater temperature2013

    • Author(s)
      Oh, M.-J., H.J. Gye, T. Nishizawa
    • Journal Title

      Vaccine

      Volume: Vol. 31 Issue: 16 Pages: 2025-2027

    • DOI

      10.1016/j.vaccine.2013.02.049

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nishizawa, Change in infectivity titre of nervous necrosis virus (NNV) in brain tissue of sevenband grouper, Epinephalus fasciatus Thunberg, with Poly(I:C) administration2013

    • Author(s)
      Oh, M-J., W-S. Kim, H-G. Seo, H-J. Gye, T
    • Journal Title

      J. Fish Dis

      Volume: Vol. 36 Issue: 2 Pages: 159-162

    • DOI

      10.1111/j.1365-2761.2012.01451.x

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] カキヘルペスウイルス1型変異株 (OsHV-1 μvar)について2013

    • Author(s)
      笠井久会
    • Journal Title

      かき研究所ニュース

      Volume: 28 Pages: 5-8

    • NAID

      120005476109

    • Related Report
      2012 Annual Research Report
  • [Journal Article] さけますふ化場で問題となる疾病の実態と対策2013

    • Author(s)
      浦和茂彦
    • Journal Title

      SALMON 情報

      Volume: 461 Pages: 1-5

    • Related Report
      2012 Annual Research Report
  • [Journal Article] 魚へのプロバイオティクス投与に期待されるもの2013

    • Author(s)
      吉水 守
    • Journal Title

      育てる漁業

      Volume: 461 Pages: 3-7

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Potentiality of a live vaccine with nervous necrosis virus (NNV) for sevenband grouper Epinephelus septemfasciatus at a low rearing temperature2012

    • Author(s)
      Nishizawa, T., H-J. Gye, I. Takami, M-J.Oh
    • Journal Title

      Vaccine

      Volume: Vol. 30 Issue: 6 Pages: 1056-1063

    • DOI

      10.1016/j.vaccine.2011.12.033

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Field tests of Poly(I:C) immunization with nervous necrosis virus (NNV) in sevenband grouper, Epinephelus septemfasciatus (Thunberg)2012

    • Author(s)
      Oh, M-J., I. Takami, T. Nishizawa, W-S.Kim, C-S. Kim, S-R. Kim, M-A. Park
    • Journal Title

      J. Fish Dis

      Volume: Vol. 35 Issue: 3 Pages: 187-191

    • DOI

      10.1111/j.1365-2761.2011.01334.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Toxicity of Poly(I:C) against Japanese Flounder <i>Paralichthys olivaceus</i>2012

    • Author(s)
      松井崇憲
    • Journal Title

      Fish Pathology

      Volume: 47 Issue: 3 Pages: 104-106

    • DOI

      10.3147/jsfp.47.104

    • NAID

      10031040449

    • ISSN
      0388-788X, 1881-7335
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RSIV is probably insensitive to the transient innate immune response induced by administration of Poly(I:C), a synthetic double-stranded RNA2012

    • Author(s)
      Kim J.-O.
    • Journal Title

      Fish Pathology

      Volume: 47 Pages: 137-142

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 魚類ウイルス病とその防除・防疫に関する研究2012

    • Author(s)
      吉水 守
    • Journal Title

      日本水産学会誌

      Volume: 78 Pages: 358-367

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Intra-ovum infection in salmonid eggs artificially contaminated with fish pathogenic bacteria: Flavobacterium psychrophilum, Renibacterium salmoninarum and Aeromonas salmonicida2012

    • Author(s)
      Kohara, M.
    • Journal Title

      Fish Pathology

      Volume: 47 Pages: 49-55

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Inactivation of koi-herpesvirus (KHV) in water using bacteria isolated from carp intestines and carp habitats2012

    • Author(s)
      Yoshida, N., et al
    • Journal Title

      Journal of Fish Diseases

      Volume: 35(in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Live vaccine of viral hemorrhagic septicemia virus (VHSV) for Japanese flounder at fish rearing temperature of 21 C instead of Poly(I:C)administration2011

    • Author(s)
      Nishizawa, T., I. Takami, M. Yanga, M-J.Oh
    • Journal Title

      Vaccine

      Volume: Vol.29 Issue: 46 Pages: 8397-8404

    • DOI

      10.1016/j.vaccine.2011.08.032

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Required dose of fish nervous necrosis virus (NNV) for Poly(I:C) immunization of sevenband grouper Epinephelus septemfasciatus2011

    • Author(s)
      Nishizawa, T., et al
    • Journal Title

      Aquaculture

      Volume: 311 Issue: 1-4 Pages: 100-104

    • DOI

      10.1016/j.aquaculture.2010.12.009

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Absorbability of bacteria isolated from active sludge of fish rearing waste water treatment facilities2011

    • Author(s)
      吉田夏子, 他
    • Journal Title

      NIPPON SUISAN GAKKAISHI

      Volume: 77 Issue: 1 Pages: 94-96

    • DOI

      10.2331/suisan.77.94

    • NAID

      10027411727

    • ISSN
      0021-5392, 1349-998X
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular identification and transmission studies of X-cell parasites from Atlantic cod Gadus morhua (Gadiformes : Gadidae) and the northern black flounder Pseudopleuronectes obscures (Pleuronectiformes : Pleuronecti-dae)2011

    • Author(s)
      Freeman, M.A., et al
    • Journal Title

      Parasites & Vectors

      Volume: 4 Issue: 1 Pages: 15-28

    • DOI

      10.1186/1756-3305-4-15

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Elimination of Escherichia coli from oysters using electrolyzed seawater2011

    • Author(s)
      Kasai, H., et al
    • Journal Title

      Aquaculture

      Volume: 319 Issue: 3-4 Pages: 315-318

    • DOI

      10.1016/j.aquaculture.2011.07.025

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 増養殖現場に求められる防疫対策で求められる数値2011

    • Author(s)
      吉水守
    • Journal Title

      アクアネット

      Volume: 14(9) Pages: 30-36

    • Related Report
      2011 Annual Research Report
  • [Journal Article] 漁場・漁港・産地市場における水産物の衛生管理基準2011

    • Author(s)
      吉水守
    • Journal Title

      アクアネット

      Volume: 14(9) Pages: 39-47

    • Related Report
      2011 Annual Research Report
  • [Journal Article] Protection of Japanese flounder Paralichthys olivaceus from viral hemorrhagic septicemia (VHS) byPoly(I:C) immunization2010

    • Author(s)
      Takami. I., S-R. Kwon, T. Nishizawa, M. Yoshimizu
    • Journal Title

      Dis. Aquat. Org

      Volume: Vol. 89 Pages: 109-115

    • DOI

      10.3354/dao02185

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protection of Japanese flounder Paralichthys olivaceus from viral hem orrhagic senticemia (VHS) by Poly (I:C) immunization.2010

    • Author(s)
      Takami, I., et al.
    • Journal Title

      Diseases of Aquatic Organisms

      Volume: 89 Pages: 109-115

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of specific antibodies against red seabream iridovirus (RSIV) in yellowtail Seriola quinaueradiata using ELISA.2010

    • Author(s)
      Kwon, S.R., et al.
    • Journal Title

      Fish Pathology

      Volume: 45: Pages: 73-76

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Renibacterium salmoninarumの表在抗原p57をELISA用抗原とした抗体検出2010

    • Author(s)
      松井崇憲, 他
    • Journal Title

      魚病研究

      Volume: 45 Pages: 80-83

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 養殖サケ科魚類の人工採卵における等調液洗卵法の除菌効果2010

    • Author(s)
      小原昌和, 他
    • Journal Title

      水産増殖

      Volume: 58 Pages: 37-43

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] コラーゲン・テクノロジーの新展開:生物種の遠い魚と人-安全性の追求2010

    • Author(s)
      吉水守
    • Journal Title

      Materials Integration

      Volume: 23 Pages: 62-66

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Prospective studies for control of viral disease of salmonid fish and flounders2010

    • Author(s)
      Yoshimizu, M.
    • Journal Title

      Proceedings of the 9^<th> Japan-Korea Joint Seminar on Fisheries Sciences

      Pages: 121-124

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Fish-immunization using a synthetic double-stranded RNA Poly(I:C), an interferon inducer, offers protection against RGNNV, a fish nodavirus2009

    • Author(s)
      Nishizawa, T., I. Takami, Y. Kokawwa, M. Yoshimizu
    • Journal Title

      Dis. Aquat. Org

      Volume: Vol. 83 Pages: 115-122

    • DOI

      10.3354/dao02001

    • NAID

      120005323614

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Virulence Change of Infectious Hematopoietic Necrosis Virus against Rainbow Trout Oncorhynchus mykiss with Viral Molecular Evolution2009

    • Author(s)
      Mochizuki, M., H.J. Kim, H. Kasai, T.Nishizawa, M. Yoshimizu
    • Journal Title

      Fish Pathology

      Volume: 44 Issue: 4 Pages: 159-165

    • DOI

      10.3147/jsfp.44.159

    • NAID

      10026105793

    • ISSN
      0388-788X, 1881-7335
    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protection of rainbow trout from infectious hematopoietic necrosis (IHN) by injection of infectious pancreatic necrosis virus (IPNV) or Poly(I:C)2009

    • Author(s)
      Kim, H-J, N. Oseko, T. Nishizawa, M.Yoshimizu
    • Journal Title

      Dis. Aquat. Org

      Volume: Vol. 83 Pages: 105-113

    • DOI

      10.3354/dao02000

    • NAID

      120005323613

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Protection of rainbow trout from infectious hematopoietic necrosis(IHN)by injection of infectious pancreatic necrosis virus(IPNV)or Poly(I:C)2009

    • Author(s)
      Kim, H.J., et al.
    • Journal Title

      Diseases of Aquatic Organisms 83

      Pages: 105-113

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fish-immunization using a synthetic double-stranded RNA Poly(I:C), an interferon inducer, offers protection against RGNNV, a fish nodavirus2009

    • Author(s)
      Nishizawa, T., et al.
    • Journal Title

      Diseases of Aquatic Organisms 83

      Pages: 115-122

    • NAID

      120005323614

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lmphocystis disease virus persists in the epidermal tissues of olive flounder, Paralichthys olivaceus(Temminch & Schlegel), at low temperatures2009

    • Author(s)
      Hossain, M., et al.
    • Journal Title

      Journal of Fish Diseases 32

      Pages: 669-703

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Duration and booster effect of phylactic response against white spot syndrome virus infection in kuruma shrimp orally administrated with recombinant viral proteins, rVP26 and rVP282009

    • Author(s)
      Satoh, J., et al.
    • Journal Title

      Fish Pathology 44

      Pages: 120-127

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Virulence change of infectious hematopoietic necrosis virus against rainbow trout Oncorhynchus mykiss with viral molecular evolution2009

    • Author(s)
      Mochizuki, M., et al.
    • Journal Title

      Fish Pathology 44

      Pages: 159-165

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Integration of a target gene into chromosomal genome of BF-2 cells using UV-inactivated snakehead retrovirus(SnRV)2009

    • Author(s)
      Kwon, S.R., et al.
    • Journal Title

      Journal of Fish Pathology 22

      Pages: 375-382

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 魚類ウイルス病とその防除・ 防疫に関する研究2013

    • Author(s)
      吉水守
    • Organizer
      日本水産学会受賞講演
    • Place of Presentation
      東京海洋大学
    • Related Report
      2012 Final Research Report
  • [Presentation] 感染耐過ニジマスが認識するOMVのタンパク質について2013

    • Author(s)
      石戸谷純貴
    • Organizer
      日本魚病学会
    • Place of Presentation
      日本大学生物資源科学部(神奈川県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] PAV防除のための電解海水を用いたクルマエビ受精卵の洗浄効果2013

    • Author(s)
      佐藤 純
    • Organizer
      日本水産学会
    • Place of Presentation
      東京海洋大学(東京都)
    • Related Report
      2012 Annual Research Report
  • [Presentation] サケ科魚ヘルペスウイルスOncorhynchus masou virus (OMV)の全ゲノム解析2012

    • Author(s)
      森真希, 他
    • Organizer
      平成24年度日本魚病学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Year and Date
      2012-03-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Comprehensive genome sequence analysis of Oncorhynchus masou virus isolated from masu, chum and rainbow trout2012

    • Author(s)
      Kasai, H.
    • Organizer
      Aqua12, Global Aquaculture
    • Place of Presentation
      プラハ国際会議場(チェコ)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 抗カキヘルペスウイルス活性を有するカキの腸内細菌の探索2012

    • Author(s)
      笠井久会
    • Organizer
      日本魚病学会
    • Place of Presentation
      水産大学校(山口県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] サケを対象にした BKD 研究の必要性2012

    • Author(s)
      吉水 守
    • Organizer
      日本魚病学会
    • Place of Presentation
      水産大学校(山口県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ウイルス性神経壊死症ウイルスに対する抗体検査の高精度化2012

    • Author(s)
      小川真依
    • Organizer
      日本水産増殖学会
    • Place of Presentation
      長崎大学(長崎県)
    • Related Report
      2012 Annual Research Report
  • [Presentation] サケの細菌性腎臓病原因菌に対する感受性および親魚の抗体保有状況2012

    • Author(s)
      笠井久会
    • Organizer
      サケ学研究会
    • Place of Presentation
      北海道大学水産学部(北海道)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 麻酔がコイの血清化学成分に与える影響2012

    • Author(s)
      金子智博
    • Organizer
      日本水産学会北海道支部会
    • Place of Presentation
      東京農業大学生物産業学部(北海道)
    • Related Report
      2012 Annual Research Report
  • [Presentation] 魚類ウイルス病とその防疫・防除に関する研究2012

    • Author(s)
      吉水守
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Vibrio neptunis 1-76株の産生する抗ウイルス活性物質の精製2012

    • Author(s)
      笠井久会, 他
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)
    • Related Report
      2011 Annual Research Report
  • [Presentation] カキの腸内細菌によるノロウイルス感染症の抑止2012

    • Author(s)
      笠井久会
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 抗ウイルス活性細菌による水産プロバイオティクスの新展開2012

    • Author(s)
      吉水守
    • Organizer
      平成24年度日本水産学会春季大会
    • Place of Presentation
      東京海洋大学(東京)(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Utilization of Poly(I:C)- immunization for viral nervous necrosis in sevenband grouper2011

    • Author(s)
      Gye, H.J., I. Takami, M.-J. Oh, T.Nishizawa
    • Organizer
      In "5th Vaccine and ISV Annual globalCongress", Seattle Marriott waterfront Hotel
    • Place of Presentation
      Seattle, USA
    • Related Report
      2012 Final Research Report
  • [Presentation] Improvement of aquaculture wastewater using sludge disposal technology2011

    • Author(s)
      Kasai H., et al.
    • Organizer
      The 9th Asian Fisheries and Aquaculture Forum
    • Place of Presentation
      上海海洋大学(中国)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Survival of norovirus (NoV) and FCV in marine environments and prediction for elimination of NoV infectivity2011

    • Author(s)
      Kasai, H., et al.
    • Organizer
      International Union of Microbiological Society 2011 Sapporo Congress
    • Place of Presentation
      札幌コンベンションホール(北海道)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Survivability of koi Herpesvirus (KHV) and a new approach for disinfection of KHV in fish breeding effluent water using anti-KHV bacteria2011

    • Author(s)
      Yoshida, N., et al.
    • Organizer
      International Union of Microbiological Society 2011 Sapporo Congress
    • Place of Presentation
      札幌コンベンションホール(北海道)
    • Related Report
      2011 Annual Research Report
  • [Presentation] カキの殻むきへの高静水圧処理の利用およびカキ体内の病原微生物の殺菌・不活化2011

    • Author(s)
      笠井久会・吉水守
    • Organizer
      第17回生物関連高圧研究会シンポジウム
    • Place of Presentation
      朱鷺メッセ新潟コンベンションセンター(新潟)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Disease problems of eel culture in Japan2011

    • Author(s)
      Tanaka, M., Yoshimizu, M.
    • Organizer
      15th Interanational Conference of European Association of Fish Pathologist
    • Place of Presentation
      Radisson Blu Hotel(クロアチア)(invited)
    • Related Report
      2011 Annual Research Report
  • [Presentation] ウイルス性神経壊死症ウイルスに対する抗体検出ELISAの改良を目的としたマツカワおよびヒラメのIgMの精製2011

    • Author(s)
      小川真依, 他
    • Organizer
      平成23年度日本魚病学会秋季大会
    • Place of Presentation
      長崎大学(長崎市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Control strategy for viral diseases of salmonid fish and flounders at hatchery or seed production facility, and net-pen or tank in Japan2011

    • Author(s)
      Yoshimizu, M., Kasai, H.
    • Organizer
      Cage Aquaculture in Asia 3
    • Place of Presentation
      Putra World Trade Centre(マレーシア)
    • Related Report
      2011 Annual Research Report
  • [Presentation] ヒラメおよびマハタの死後変化に及ぼす致死条件の影響2011

    • Author(s)
      笠井久会, 他
    • Organizer
      平成23年度日本水産学会北海道支部会
    • Place of Presentation
      北海道大学(函館市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] サクラマスにおける細菌性腎臓病の垂直感染の防止試験2011

    • Author(s)
      畑山誠, 他
    • Organizer
      第5回サケ学研究会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Detection methods for epizootiological survey of Aeromonas salmonicida, the causative agent of furunculosis2011

    • Author(s)
      Devon Dublin, et al.
    • Organizer
      第5回サケ学研究会
    • Place of Presentation
      北海道大学(札幌市)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 魚細胞におけるバキュロウイルスベクターシステムの開発に向けて2010

    • Author(s)
      横尾成子, 他
    • Organizer
      分子生物学会
    • Place of Presentation
      神戸ポートアイランド(神戸市)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Efficacy of Poly (I:C) immunization for IHN, VNN and VHS.2010

    • Author(s)
      Kwon S-R., et al.
    • Organizer
      韓国魚病学会
    • Place of Presentation
      Pusan (Korea)
    • Year and Date
      2010-11-12
    • Related Report
      2010 Annual Research Report
  • [Presentation] Survivability of Koi Herpesvirus (KHV) and a new approach for disinfection of KHV in fish breeding effluent water using anti-KHV bacteria2010

    • Author(s)
      Yoshida, N., et al.
    • Organizer
      日本水産増殖学会
    • Place of Presentation
      唐津市民プラザ(唐津市)
    • Year and Date
      2010-10-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] マハタ稚魚のウイルス性神経壊死症防除における殺菌海水飼育の必要性2010

    • Author(s)
      渡邊研一, 他
    • Organizer
      日本魚病学会
    • Place of Presentation
      三重大学(津市)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] マハタのPoly(1:C)免疫法における最小必要ウイルス量2010

    • Author(s)
      高見生雄, 他
    • Organizer
      日本魚病学会
    • Place of Presentation
      三重大学(津市)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] KDM-SMRs 培地によるRenibacterium salmoninarum生菌数の測定2010

    • Author(s)
      吉水守
    • Organizer
      日本魚病学会
    • Place of Presentation
      三重大学(津市)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞の増殖およびCPE形成時の形態変化2010

    • Author(s)
      森真希, 他
    • Organizer
      日本魚病学会
    • Place of Presentation
      三重大学(津市)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 魚類病原細菌および一般細菌の人工感染試験による卵内感染機序の検討2010

    • Author(s)
      小原昌和
    • Organizer
      日本魚病学会
    • Place of Presentation
      三重大学(津市)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] 等張液洗卵法による病原細菌およびウイルスの除菌・除去効果と卵内感染防除効果2010

    • Author(s)
      小原昌和
    • Organizer
      日本魚病学会
    • Place of Presentation
      三重大学(津市)
    • Year and Date
      2010-09-22
    • Related Report
      2010 Annual Research Report
  • [Presentation] Trends in aquatic animal health in Japan2010

    • Author(s)
      Ogawa, K., M. Yoshimizu
    • Organizer
      American Fisheries Society
    • Place of Presentation
      Tampa, Florida (USA) 招待講演
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Occurrence of intra-ovum infection and effect of egg-washing with isotonic solution on elimination of bacteria and viruses before fertilization in salmon aquaculture.2010

    • Author(s)
      Kohara, M., et al.
    • Organizer
      American Fisheries Society
    • Place of Presentation
      Tampa, Florida (USA)
    • Year and Date
      2010-09-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] Efficacy of Poly (I:C) immunization for IHN, VNN and VHS.2010

    • Author(s)
      Nishizawa T., et al.
    • Organizer
      8^<th> International Symposium Viruses of Lower Vertebrates
    • Place of Presentation
      Santiago de Compostela (Spain)
    • Year and Date
      2010-04-27
    • Related Report
      2010 Annual Research Report
  • [Presentation] Poly(I:C) immunization for fish virus diseases2010

    • Author(s)
      Nishizawa, T., I. Takami, S.R. Kwon, M.Yoshimizu, M-J. Oh
    • Organizer
      In"The 8th International Symposium of Viruses of Lower Vertebrates " , Universidad de Santiago de Compostela
    • Place of Presentation
      Spain
    • Related Report
      2012 Final Research Report
  • [Presentation] Survivability of koi Herpesvirus (REV) and a new approach for disinfection of KHV in fish breeding effluent water using anti-KHV bacteria.2010

    • Author(s)
      Yoshida, N., et al.
    • Organizer
      American Fisheries Society
    • Place of Presentation
      Tampa, Florida (USA)
    • Related Report
      2010 Annual Research Report
  • [Presentation] Measurement of viable Renibacterium salmoninarum counts by modified KDM-2 with culture-spent medium.2010

    • Author(s)
      Yoshimizu, M., et al.
    • Organizer
      American Fisheries Society
    • Place of Presentation
      Tampa, Florida (USA)
    • Related Report
      2010 Annual Research Report
  • [Presentation] WSSV rVP26およびrVP28の発現条件と経口ワクチンとしての最少有効投与量2009

    • Author(s)
      佐藤純, ら
    • Organizer
      日本魚病学会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2009-09-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] ヒラメのVHSにおけるpoly(I:C)-免疫法の有効性2009

    • Author(s)
      高見生雄, ら
    • Organizer
      日本魚病学会
    • Place of Presentation
      東北大学(仙台)
    • Year and Date
      2009-09-28
    • Related Report
      2009 Annual Research Report
  • [Presentation] インターフェロン誘導dsRNAウイルスおよび合成2本鎖RNA Poly(I:C)を用いた魚類の免疫法2009

    • Author(s)
      權世蓮, ら
    • Organizer
      日本ウイルス学会北海道支部大会
    • Place of Presentation
      北海道十勝川温泉
    • Year and Date
      2009-07-25
    • Related Report
      2009 Annual Research Report
  • [Book] 『最新 水産ハンドブック』第3章7節. 疾病と防除,4.診断2012

    • Author(s)
      吉水 守
    • Publisher
      講談社
    • Related Report
      2012 Annual Research Report
  • [Book] Chapter 7 : Oncogenic viruses, 『2nd edition of Fish Diseases & Disorders, Vol. 3』2011

    • Author(s)
      Yoshimizu, M., Kasai, H.
    • Publisher
      CABI International
    • Related Report
      2011 Annual Research Report
  • [Book] 水産利用化学の基礎2010

    • Author(s)
      渡部終五編
    • Total Pages
      211
    • Publisher
      恒星社厚生閣
    • Related Report
      2010 Annual Research Report
  • [Patent(Industrial Property Rights)] 魚類ウイルスワクチン、魚類免疫賦活剤、および魚類免疫付与法2010

    • Inventor(s)
      西澤豊彦・吉水守・高見生雄
    • Industrial Property Rights Holder
      北海道大学
    • Industrial Property Number
      2010-526739
    • Filing Date
      2010-12-22
    • Related Report
      2010 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi