| Project/Area Number |
21380116
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKAGI Yasuaki 北海道大学, 大学院・水産科学研究院, 教授 (10212002)
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| Co-Investigator(Kenkyū-buntansha) |
IKOMA Toshiyuki 東京工業大学, 大学院・理工学研究科, 准教授 (20370306)
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| Co-Investigator(Renkei-kenkyūsha) |
URA Kazuhiro 北海道大学, 大学院・水産科学研究院, 助教 (90360940)
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| Project Period (FY) |
2009 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2009: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
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| Keywords | 生理 / 生体材料 / 鱗 / 再生 / コラーゲン / 角膜 / 魚類コラーゲン / コラーゲン配向 / 変性温度 / SLRPs / 人工骨 / 人工角膜 / 生体修復材料 / 非コラーゲン性基質タンパク質 / 再生医療用デバイス |
|---|
| Research Abstract |
Using MS analysis, scale SLRPs, which were possible regulators of 3-dimensinal architecture of teleost fish collagen, were identified. After the cDNA cloning of SLRPs, localization of their mRNAs were clarified. We also revealed a negative correlation between collagen denaturation temperature (DT) and contents of α3-subunit, as well as positive correlation between DT and hydroxylation ratio of proline residues in the collagen. Thus, these two factors seem to be the major regulators of DT of fish collagen. Artificial fibrillogenesis of collagen under magnetic field enabled a synthesis of a material having relatively aligned collagen fibrils, whose viscoelasticity and tensile strength were highest under 4 T and 12 T, respectively. High-density meso-porous new material was also synthesized using fish collagen.
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