| Project/Area Number |
21380123
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General fisheries
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HAMASAKI Koji 東京大学, 大気海洋研究所, 准教授 (80277871)
YOSHIZAWA Susumu 東京大学, 大気海洋研究所, 特任研究員 (00553108)
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| Research Collaborator |
PARK Sanghwa 東京大学, 大気海洋研究所, 農学特定研究員
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2011: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Keywords | プロテオロドプシン / 海洋細菌 / 光エネルギー / プロトンポンプ活性 / Flavobacteria / 遺伝子解析 / 遺伝子伝搬 / 16S rDNA / Bacteroidetes / エルギー代謝 / 海洋生態系 / 系統解析 / エネルギー代謝 |
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| Research Abstract |
Proteorhodopsin (PR) is a membrane protein that absorbs light energy and functions as proton pump. The proton gradient thus established enables the cells to synthesize ATP, thus serves as efficient energy synthesis component. This was first reported in 2000. Since then there have been estimated that nearly half of prokaryotes present in the upper water column in the ocean may possess PR. However, so far only about 10 isolates had been reported to possess PR, that makes any further physiological investigations difficult. Our group succeeded to isolate more than 60 strains and directly measure the activity of proton pump under light. In addition, with further assumptions we succeeded to estimate the contribution of PR to light energy absorption to the ocean for the first time. The research on PR is now expanding after our investigation using isolates.
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