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Biochemical studies on of ecological function of meiobenthos

Research Project

Project/Area Number 21380131
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Fisheries chemistry
Research InstitutionKyoto University

Principal Investigator

TOYOHARA Haruhiko  京都大学, 農学研究科, 准教授 (90183079)

Co-Investigator(Kenkyū-buntansha) KASAI Akihide  京都大学, 農学研究科, 准教授 (80263127)
Co-Investigator(Renkei-kenkyūsha) KAMITAKAHARA Hiroshi  京都大学, 農学研究科, 准教授 (10293911)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2009: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywordsセルラーゼ / セルロース / メイオベントス / 底泥 / 湿地帯 / マングローブ / ベントス / 干潟 / 河川 / 河川(底泥
Research Abstract

To determine the process of cellulose degradation in mangrove estuaries of the South-Western Islands, Japan, the cellulase activity of sediments was examined at a total of 9 sites on Okinawa Main Island (the River Gesashi and Manko), Ishigaki Island (Nagura Gulf and River Miyara), and Iriomote Island (rivers Urauchi, Mare, Hinai, Shira and Maira). Zymographic analysis was used to record cellulase activity in all sediment samples and in the meiobenthos. A 27-kDa cellulase activity band was expressed by several animal groups examined in the meiobenthos, including Gammaridea, Turbellaria, Ostracoda, Oligochaeta, Nematoda, and Polychaeta. However, the mangrove whelk, Terebralia palustris, which is a widely distributed member of the macrobenthos in these areas except Okinawa Main Island, expressed cellulase activities at 29, 31, 49, and 56 kDa. Interestingly, 31-kDa cellulase activity levels were recorded both in sediments and in the feces of T. palustris. The results of this study indicate that various benthic animals contribute to cellulose degradation in mangrove sediments of estuaries in the Southwestern Japanese Islands.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (24 results)

All 2013 2012 2011 2010 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (15 results) Remarks (4 results)

  • [Journal Article] Animal cellulases with a focus on aquatic invertebrates2013

    • Author(s)
      A.Tanimura, W. Limu, K.Yamada, T. Kishida, H.Toyohara
    • Journal Title

      Fish Sci

      Volume: 79 Pages: 1-13

    • NAID

      10031138636

    • Related Report
      2011 Final Research Report
  • [Journal Article] 1. Distribution of cellulase activities among the Acetes shrimps living in the Matang Mangrove Forest Reserve, Malaysia.2013

    • Author(s)
      Aya Tanimura
    • Journal Title

      Malaysian Journal of Science

      Volume: 44 Pages: 12-17

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Animal Cellulases with a Focus on Aquatic Invertebrates2013

    • Author(s)
      Aya Tanimura
    • Journal Title

      Fish Sci

      Volume: 79 Pages: 1-13

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Occurrence of cellulase activities in mangrove estuarine mysids and Acetes shrimps2012

    • Author(s)
      T. Niiyama, Y. Hanamura, K. Tanaka and H. Toyohara
    • Journal Title

      JIRCUS Working Report

      Volume: 75 Pages: 35-40

    • Related Report
      2011 Final Research Report
  • [Journal Article] Widespread distribution of cellulase and hemi-cellulase activities among aquatic invertebrates

    • Author(s)
      Takaaki Niiyama, Haruhiko Toyohara
    • Journal Title

      Fisheries Sciences

      Volume: (印刷中)

    • NAID

      10029123974

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 底泥バイオリアクターシステムにおけるベントス排泄物由来酵素の役割2013

    • Author(s)
      豊原治彦
    • Organizer
      日本水産学会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
  • [Presentation] Validation of the breakdown activity of secreted cellulases from Corbicula japonica in sediment bioreactor system2013

    • Author(s)
      Wen Liu
    • Organizer
      日本水産学会
    • Place of Presentation
      東京
    • Related Report
      2011 Annual Research Report
  • [Presentation] Validation of the breakdown activity of secreted cellulases from Corbicula japonica in sediment bioreactor system. (底泥バイオリアクター系におけるヤマトシジミの分泌性セルラーゼの分解機能の検証)2012

    • Author(s)
      W.Liu and H.Toyohara
    • Organizer
      平成 24 年度日本水産学会秋季大会
    • Related Report
      2011 Final Research Report
  • [Presentation] 底泥バイオリアクターシステムにおけるベントス排泄物由来酵素の役割2012

    • Author(s)
      豊原治彦・山田京平・谷村彩・劉文
    • Organizer
      平成 24 年度日本水産学会秋季大会
    • Related Report
      2011 Final Research Report
  • [Presentation] Probiotic shrimp culture in Japan and Thailand - Utilization of Enzymes from Traditional Fermented Foods2012

    • Author(s)
      H.Toyohara, W.Liu, Xuegang Li, Y.Araki, W.Salikput, K.Shiomi
    • Organizer
      JSPS-NRCT Asian Core Program Symposium 2012
    • Related Report
      2011 Final Research Report
  • [Presentation] Determination of the "wetland sediments acting as bioreactors" hypothesis from the standpoint of the mechanism of Cellulose Binding Domain (CBD) of a cellulase of a brackish clam Corbicula japonica.2012

    • Author(s)
      W.Liu, S.Maegawa, H.Toyohara.
    • Organizer
      JSPS-NRCT Asian Core Program Symposium 2012
    • Related Report
      2011 Final Research Report
  • [Presentation] Distribution of cellulase activities among the animals living in the Matang mangrove forest reserve2012

    • Author(s)
      Malaysia. A,Tanimura, T.Niiyama, H.Toyohara, Y.Hanamura, K.Tanaka
    • Organizer
      Synposium on South China Sea 2012
    • Related Report
      2011 Final Research Report
  • [Presentation] 干潟底泥バイオリアクター説の検証ーヤマトシジミセルラーゼのcellulose binding domain (CBD)のアンカー機能の検討2012

    • Author(s)
      劉文
    • Organizer
      日本水産学会
    • Place of Presentation
      下関
    • Related Report
      2011 Annual Research Report
  • [Presentation] DISTRIBUTION OF CELLULASE ACTIVITIES AMONG THE ANIMALS LIVING IN THE MATANG MANGROVE FOREST RESERVE, MALAYSIA2012

    • Author(s)
      Aya Tanimura
    • Organizer
      南シナ海学会
    • Place of Presentation
      クアラルンプル
    • Related Report
      2011 Annual Research Report
  • [Presentation] 湿地帯における 植物起源有機物の分解機構2011

    • Author(s)
      豊原治彦
    • Organizer
      日本木材学会
    • Place of Presentation
      京都大学(招待講演)
    • Year and Date
      2011-03-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 湿地帯における 植物起源有機物の分解機構2011

    • Author(s)
      豊原治彦
    • Organizer
      日本木材学会
    • Place of Presentation
      京都大学(京都市)
    • Year and Date
      2011-03-20
    • Related Report
      2009 Annual Research Report
  • [Presentation] Biochemical Studies on the Cellulase Activities in Macrobenthos in a Mangrove Estuary2010

    • Author(s)
      Haruhiko Toyohara
    • Organizer
      JRCUS Work shop "Sustainable stock management and aquaculture technology suitable for Southeast Asia"
    • Place of Presentation
      つくば国際会議場(招待講演)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Enzymes Involved in the Degradation of Plant-Derived Carbohydrates in the Wet Lands2010

    • Author(s)
      Haruhiko Toyohara, Kentaro Sakamoto
    • Organizer
      9th International Marine Biotechnology Conference
    • Place of Presentation
      チンタオ(中国)
    • Year and Date
      2010-10-11
    • Related Report
      2010 Annual Research Report 2009 Annual Research Report
  • [Presentation] 湿地帯の浄化機能に関する生化学的研究-4.泥粒子のセルラーゼ固定化能の検討2010

    • Author(s)
      劉文, 豊原治彦
    • Organizer
      日本水産学会 秋季大会
    • Place of Presentation
      京都大学
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] Cellulase activity of ocypodid crabs on the Tanakagawa tidal flat, central Japan

    • Author(s)
      S.Kawaida, H.Toyohara, T.Kimura
    • Organizer
      The First Asian Marine Biology Symposium
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.mbf.marine.kais.kyoto-u.ac.jp/

    • Related Report
      2011 Final Research Report
  • [Remarks] 海洋生物機能学分野

    • URL

      http://www.mbf.marine.kais.kyoto-u.ac.jp/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.mbf.marine.kais.kyoto-u.ac.jp/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.mbf.marine.kais.kyoto-u.ac.jp/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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