| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
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| Research Abstract |
The purpose of this research is to clarify the relationship between nitrate concentration and variation in nitrate reductase(NR) gene expression. The tested plants were komatsuna. Absorption spectra at each pixel on leaf surface were measured by using a hpyerspectral imaging system. In order to derive mathematical models to estimate the nitrate concentration from absorption spectra, mean spectrum over pixels that were included in small leaf bit was measured. Nitrate concentration of the leaf bit was measured by HPLC. This measurement was applied to 459 leaf bits that included leaf blades and midribs. The mathematical models were developed for leaf blade or midrib separately. Correlation coefficient between the measured and estimated concentration of evaluation data showed more than 0. 91 in both the models. These models were applied to visualize the distribution image of nitrate concentration in a leaf. Then sections of high or low concentration were detected and were cut off from the leaf. Variations of NR gene expression in the leaf sections were measured by PT-PCR and agarose gel electrophoresis methods. Results of this analysis showed that there was not clear correlation between the nitrate concentration and variation of NR gene expression. However, variations of NR gene expression tended to increase with increment of nitrate concentration from initial concentration. It was clarified that the increment of nitrate concentration was considered as one of the factors to induce NR gene expression.
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