Identification of Signaling Transmitter to Activate Nitrate Reductase in Vegetable leaves by Near-Infrared Hyperspectral Imaging System
| Project/Area Number |
21380156
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Agricultural environmental engineering
|
|---|
| Research Institution | Kobe University |
|---|
Principal Investigator |
ITOH Hiromichi 神戸大学, 大学院・農学研究科, 准教授 (00258063)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIRAISHI Naomasa 神戸大学, 大学院・農学研究科, 助教 (00304121)
UNO Yuichi 神戸大学, 大学院・農学研究科, 准教授 (90304120)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2009: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
|
|---|
| Keywords | 施設園芸 / 植物工場 / 葉面内硝酸イオン濃度分布 / ハイパースペクトルカメラ / 近赤外線分光法 / RT-PCR法 / 電気泳動 / 硝酸還元酵素遺伝子 / mRNA / パルスチェイス法 / 葉菜中硝酸イオン濃度分布 / 主成分回帰法 / PLS法 / ホウレンソウ / コマツナ |
|---|
| Research Abstract |
The purpose of this research is to clarify the relationship between nitrate concentration and variation in nitrate reductase(NR) gene expression. The tested plants were komatsuna. Absorption spectra at each pixel on leaf surface were measured by using a hpyerspectral imaging system. In order to derive mathematical models to estimate the nitrate concentration from absorption spectra, mean spectrum over pixels that were included in small leaf bit was measured. Nitrate concentration of the leaf bit was measured by HPLC. This measurement was applied to 459 leaf bits that included leaf blades and midribs. The mathematical models were developed for leaf blade or midrib separately. Correlation coefficient between the measured and estimated concentration of evaluation data showed more than 0. 91 in both the models. These models were applied to visualize the distribution image of nitrate concentration in a leaf. Then sections of high or low concentration were detected and were cut off from the leaf. Variations of NR gene expression in the leaf sections were measured by PT-PCR and agarose gel electrophoresis methods. Results of this analysis showed that there was not clear correlation between the nitrate concentration and variation of NR gene expression. However, variations of NR gene expression tended to increase with increment of nitrate concentration from initial concentration. It was clarified that the increment of nitrate concentration was considered as one of the factors to induce NR gene expression.
|
Report
(4 results)
Research Products
(57 results)
-
-
-
-
-
-
-
-
-
[Journal Article] Development of Hyperspectral Imaging System to Measure Spatial Distribution of Nitrate Concentration in Vegetables2010
Author(s)
Kanda, S., Itoh, H., Matsuura, H., Tomoda, S., Shiraishi, N., Sakai, K., Sasao, A.
-
Journal Title
Proceedings of ISMAB 2010 JAPAN
Volume: (CD-ROM)
Pages: 6-6
Related Report
Peer Reviewed
-
[Journal Article] Measurement of Nitrate Concentration Distribution in Vegetables by Near-Infrared Hyperspectral Imaging2010
Author(s)
Itoh, H., Kanda, S., Matsuura, H., Shiraishi, N., Sakai, K. SASAO, A.
-
Journal Title
Environment Control in Biology
Volume: 48(2)
Pages: 31-43
NAID
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Measurement of nitrate concentration distribution invegetables by hyperspectral camera2009
Author(s)
Matsuura, H., Itoh, H., Kanda, S., Shiraishi, N., Sakai, K., Sasao, A.
-
Journal Title
Proceedings of BIO-ROBOTICS IIII
Volume: (CD-ROM)
Pages: 6-6
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Development of Hyperspectral Imaging System to Measure Spatial Distribution of Nitrate Concentration in Vegetables2010
Author(s)
Kanda, S., Itoh, H., Matsuura, H., Tomoda, S., Shiraishi, N., Sakai, K., Sasao, A
Organizer
International Symposium on Machinery and Mechatronics for Agriculture and Biosystems Engineering
Place of Presentation
JAPAN, Fukuoka
Year and Date
2010-04-06
Related Report
-
-
-
-
[Presentation] Measurement of nitrate concentration distribution in vegetables by hyperspectral camera2009
Author(s)
Matsuura, H., Itoh, H., Kanda, S., Shiraishi, N., Sakai K., Sasao, A.
Organizer
Bio-Robotics IV
Place of Presentation
USA
Year and Date
2009-09-10
Related Report
-
-
-
-
-
-
-
-
-
