Basic and applied studies on immunobiotics by using intestinal peyer's patch model system of live stock animals

• Project/Area Number: 21380164
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Zootechnical science/Grassland science
• Research Institution: Tohoku University
• Principal Investigator: KITAZAWA Haruki 東北大学, 大学院・農学研究科, 准教授 (10204885)
• Co-Investigator(Kenkyū-buntansha): SAITO Tadao 東北大学, 大学院・農学研究科, 教授 (00118358) ; ASO Hisashi 東北大学, 大学院・農学研究科, 教授 (50241625) ; KAWAI Yasushi 東北大学, 大学院・農学研究科, 助教 (00261496)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2009: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
• Keywords: イムノバイオティクス / 家畜 / ブタ / ウシ / 免疫 / 腸管上皮 / 腸管パイエル板 / 飼料

Research Abstract

In this study, advanced immunobiotic assay systems for anti-inflammatory and anti-viral immunity was successfully developed by using originally established intestinal epithelial cells from live stock animals. The utility of these assay systems was verified by in vivo trial of selected immunobiotic candidate by these assay system in swine. This study may contribute to develop a safer technique without any drugs for livestock production.

Research Products

• [Journal Article] Advanced application of porcine intestinal epithelial cells for the selection of immunobiotics modulating toll-like receptor 3-mediated inflammation (2012)
  • Author(s): Hosoya, S., J. Villena, E. Chiba, T. Shimazu, Y. Suda, H. Aso, T. Saito, H. Kitazawa
  • Journal Title: J. Microbiol. Immunol. Infect
  • Peer Reviewed
• [Journal Article] A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle (2012)
  • Author(s): Chiba, E., J. Villena, S. Hosoya, N. Takanashi, T. Shimazu, H. Aso, M. Tohno, Y. Suda, Y. Kawai, T. Saito, K. Miyazawa, F. He, H. Kitazawa.
  • Journal Title: Res. Vet. Sci
  • Peer Reviewed
• [Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway (2012)
  • Author(s): Shimazu, T., J. Villena, M. Tohno, H. Fujie , S. Hosoya, T. Shimosato, H. Aso, Y. Suda, Y. Kawai, T. Saito, S. Makino, S. Ikegami, H. Itoh, H. Kitazawa
  • Journal Title: Infect. Immun. Volume: 80 Pages: 276-288
  • Peer Reviewed
• [Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway (2012)
  • Author(s): Shimazu, T., H.Aso, Y.Suda, Y.Kawai, T.Saito, H.Kitazawa, et al
  • Journal Title: Infect.Immun. Volume: 80 Issue: 1 Pages: 276-288
  • DOI: 10.1128/iai.05729-11
  • Peer Reviewed
• [Journal Article] Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I : C)in porcine intestinal epithelial cells (2011)
  • Author(s): Hosoya, S., J. Villena, T. Shimazu, M. Tohno, H. Fujie, E. Chiba, T. Shimosato, H. Aso, Y. Suda, Y. Kawai, T, Saito, S. Alvarez, S. Ikegami, H. Itoh, H. Kitazawa
  • Journal Title: Vet. Res Volume: 42(111) Pages: 1-13
  • Peer Reviewed
• [Journal Article] Toll-like receptor-2 activating bifidobacteria strains differentially regulate inflammatory cytokines in porcine intestinal epithelial cell culture system : finding new anti-inflammatory immunobiotics (2011)
  • Author(s): Fujie, H., J. Villena, M. Tohno, K. Morie, T. Shimazu, H. Aso, Y. Suda, T. Shimosato, N. Iwabuchi, J. Z. Xiao, T. Yaeshima, K. Iwatsuki, T. Saito, M. Numasaki, H. Kitazawa
  • Journal Title: FEMS Immunol. Med. Microbiol Volume: 63 Pages: 129-139
  • Peer Reviewed
• [Journal Article] Immunobiotic Lactobacillus strains augment NLRP3 expression in newborn and adult porcine gut-associated lymphoid tissues (2011)
  • Author(s): Tohno, M., T. Shimosato, H. Aso, H. Kitazawa
  • Journal Title: Vet. Immunol. Immunopathol Volume: 144 Pages: 410-416
  • Peer Reviewed
• [Journal Article] Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4 (2011)
  • Author(s): Ueda, W., M. Tohno, T. Shimazu, H. Fujie, H. Aso, Y. Kawai, M. Numasaki, T. Saito, H. Kitazawa
  • Journal Title: Vet. Immunol. Immunopathol Volume: 143 Pages: 148-154
  • Peer Reviewed
• [Journal Article] 豚腸内感染症を制御するイムノバイオティクスおよびイムノジェニクスの探索 (2011)
  • Author(s): 北澤
  • Journal Title: 動物疾病と腸内細菌シリーズ Volume: 6 Pages: 1-5
• [Journal Article] Cytokeratin 18 is a specific marker of bovine intestinal microfold (M)cell (2011)
  • Author(s): Hondo, T., T. Kanaya, I. Takakura, H. Watanabe, Y. Takahashi, Y. Nagasawa, S. Terada, S. Ohwada, K. Watanabe, H. Kitazawa, M. Rose, T. Yamaguchi, H. Aso
  • Journal Title: Am. J. Physiol-Gastrointestinal and Liver Physiology Volume: 300
  • Peer Reviewed
• [Journal Article] Immunobiotic lactic acid bacteria beneficially regulate immune response triggered by poly(I:C) in porcine intestinal epithelial cells (2011)
  • Author(s): Hosoya, S., H.Aso, Y.Suda, Y.Kawai, T.Saito, H.Kitazawa, et al
  • Journal Title: Vet.Res. Volume: 42(111) Issue: 1 Pages: 1-13
  • DOI: 10.1186/1297-9716-42-111
  • Peer Reviewed
• [Journal Article] Toll-like receptor-2 activating bifidobacteria strains differentially regulate inflammatory cytokines in porcine intestinal epithelial cell culture system : finding new anti-inflammatory immunobiotics (2011)
  • Author(s): Fujie, H., H.Aso, Y.Suda, T.Saito, H.Kitazawa, et al
  • Journal Title: FEMS Immunol.Med.Microbiol. Volume: 63 Issue: 1 Pages: 129-139
  • DOI: 10.1111/j.1574-695x.2011.00837.x
  • Peer Reviewed
• [Journal Article] Immunobiotic Lactobacillus strains augment NLRP3 expression in newborn and adult porcine gut-associated lymphoid tissues (2011)
  • Author(s): Tohno, M., H.Aso, H.Kitazawa, et al
  • Journal Title: Vet.Immunol.Immunopathol. Volume: 144 Issue: 3-4 Pages: 410-416
  • DOI: 10.1016/j.vetimm.2011.09.010
  • Peer Reviewed
• [Journal Article] Molecular cloning, tissue expression, and subcellular localization of porcine peptidoglycan recognition proteins 3 and 4 (2011)
  • Author(s): Ueda, W., H.Aso, Y.Kawai, T.Saito, H.Kitazawa, et al
  • Journal Title: Vet.Immunol.Immunopathol. Volume: 143 Issue: 1-2 Pages: 148-154
  • DOI: 10.1016/j.vetimm.2011.05.026
  • Peer Reviewed
• [Journal Article] Cytokeratin 18 is a specific marker of bovine intestinal microfold (M) cell (2011)
  • Author(s): Hondo, T., H.Kitazawa, H.Aso, et al
  • Journal Title: Am.J.Physiol-Gastrointestinal and Liver Physiology Volume: 300
  • Peer Reviewed
• [Journal Article] 安全な持続的家畜生産のためのイムノバイオティック革命 (2010)
  • Author(s): 北澤、須田、麻生
  • Journal Title: 日本家畜臨床感染症研究会誌 Volume: 5 Pages: 79-83
  • Peer Reviewed
• [Journal Article] Development of Immunobiotic Evaluation System for Functional Foods and Feeds (2010)
  • Author(s): Kitazawa, H., M. Tohno, T. Shimosato, Y. Suda and H. Aso
  • Journal Title: Proceedings of the 14th AAAP Animal Science Congress, Pingtung, Taiwan, ROC, Aug Volume: Vol.1 Pages: 23-27
• [Journal Article] Characterization of newly established bovine intestinal epithelial cell line (2010)
  • Author(s): Miyazawa K., T. Hondo, T. Kanaya, S. Tanaka, I. Takakura, W. Itani, M. T. Rose, H. Kitazawa, T. Yamaguchi, H. Aso
  • Journal Title: Histochem. Cell Biol Volume: 133 Pages: 125-134
  • Peer Reviewed
• [Journal Article] Development of Immunobiotic Evaluation System for Functional Foods and Feeds (2010)
  • Author(s): Kitazawa, H., et al
  • Journal Title: Proceedings of the 14th AAAP Animal Science Congress Volume: 1 Pages: 23-27
  • Peer Reviewed
• [Journal Article] Characterization of newly established bovine intestinal epithelial cell line. (2010)
  • Author(s): Miyazawa, K., H.Kitazawa, H.Aso, et al.
  • Journal Title: Histochem.Cell Biol 133 Pages: 125-134
  • Peer Reviewed
• [Journal Article] Immunomodulation in gut-associated lymphoid tissue of neonatal chicks by immunobiotic diets. Poultry Sci (2009)
  • Author(s): Sato, K., K. Takahashi, M. Tohno, Y. Miura, S. Ikegami, H. Kitazawa
  • Volume: 88
  • Pages: 2532-2538
  • Peer Reviewed
• [Journal Article] Identification of a potent immunostimulatory oligodeoxynucleotide from the Streptococcus thermophilus lacZ (2009)
  • Author(s): Shimosato, T., M. Tohno, T. Sato, J. Nishimura, Y. Kawai, T. Saito, H. Kitazawa
  • Journal Title: Anim. Sci. J Volume: 80 Pages: 597-604
  • NAID: 10027887689
  • Peer Reviewed
• [Journal Article] パターン認識受容体を介するイムノバイオティクスの腸管免疫調節機構 (2009)
  • Author(s): 遠野、北澤
  • Journal Title: 栄養生理研究会報 Volume: 53 Pages: 19-33
  • NAID: 10024752851
  • Peer Reviewed
• [Journal Article] Modulation of innate immunity by lactic acid bacteria : impact on host response to infections. (2009)
  • Author(s): Alvarez, S., H.Kitazawa, et al.
  • Journal Title: Curr.Res.in Immunology 3 Pages: 87-126
  • Peer Reviewed
• [Presentation] ウシ対応型イムノバイオティクスの腸管炎症調節機構 (2012)
  • Author(s): 高梨、麻生、須田、川井、齋藤、北澤ら
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋
• [Presentation] ブタ対応型イムノバイオティクスの腸管モデル共培養系による抗ウイルス性免疫評価 (2012)
  • Author(s): 千葉、麻生、川井、齋藤、北澤ら
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋
• [Presentation] 抗菌剤に頼らない「イムノバオテック飼料添加物」の開発戦略 (2011)
  • Author(s): 北澤
  • Organizer: シンポジウム「家畜衛生等技術のこれから～民間の力を生かして～」
  • Place of Presentation: 名古屋
  • Year and Date: 2011-12-19
• [Presentation] 抗菌剤に頼らない「イムノバオテック飼料添加物」の開発戦略 (2011)
  • Author(s): 北澤
  • Organizer: シンポジウム「家畜衛生等技術のこれから～民間の力を生かして～」
  • Place of Presentation: ウインク愛知、名古屋
  • Year and Date: 2011-12-19
• [Presentation] ウシ腸管上皮(BIE)細胞による抗炎症性イムノバイオティクスの選抜・評価 (2011)
  • Author(s): 村田、川井、齋藤、北澤ら
  • Organizer: 日本食品免疫学会第7回学術大会
  • Place of Presentation: 東京
• [Presentation] ウシ腸管上皮細胞によるイムノバイオティクスの抗ウイルス性免疫評価 (2011)
  • Author(s): 千葉、川井、齋藤、北澤ら
  • Organizer: 日本食品免疫学会第7回学術大会
  • Place of Presentation: 東京
• [Presentation] ウシ対応型の抗炎症性イムノバイオティックスの選抜・評価 (2011)
  • Author(s): 村田、麻生、川井、齋藤、北澤ら
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田
• [Presentation] ウシ腸管上皮細胞による抗ウイルス性イムノバイオティクスの評価系構築 (2011)
  • Author(s): 千葉、麻生、川井、齋藤、北澤ら
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田
• [Presentation] 腸管免疫系におけるイムノバイオティック乳酸菌によるNLRP3受容体の発現増強 (2011)
  • Author(s): 遠野、麻生、北澤ら
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田
• [Presentation] Toll-like receptor-2 activating Bifidobacteria strains regulate inflammatory response in porcine intestinal epithelial cells (2011)
  • Author(s): Fujie, H., H. Aso, Y. Suda, Y. Kawai, T. Saito, H. Kitazawa, et al.
  • Organizer: FEMS
  • Place of Presentation: ジュネーブ、スイス
• [Presentation] Toll-like receptor 4 recognizes extracellular polysaccharide from Lactococcus lactis subsp (2011)
  • Author(s): Tohno.M., H. Aso, H. Kitazawa, et al
  • Organizer: cremoris, FEMS
  • Place of Presentation: ジュネーブ、スイス
• [Presentation] Toll-like receptor-2 activating Bifidobacteria strains regulate inflammatory response in porcine intestinal epithelial cells (2011)
  • Author(s): Fujie, H., H.Aso, Y.Suda, Y.Kawai, T.Saito, H.Kitazawa, et al
  • Organizer: FEMS2011
  • Place of Presentation: ジュネーブ、スイス
• [Presentation] Toll-like receptor 4 recognizes extracellular polysaccharide from Lactococcus lactis subsp.cremoris (2011)
  • Author(s): Tohno, M., H.Aso, H.Kitazawa, et al
  • Organizer: FEMS2011
  • Place of Presentation: ジュネーブ、スイス
• [Presentation] ウシ対応型の抗炎症性イムノバイオティックスの選抜・評価 (2011)
  • Author(s): 村田、麻生、川井、齋藤、北澤, ら
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田、青森
• [Presentation] ウシ腸管上皮細胞による抗ウイルス性イムノバイオティクスの評価系構築 (2011)
  • Author(s): 千葉、麻生、川井、齋藤、北澤, ら
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田、青森
• [Presentation] 腸管免疫系におけるイムノバイオティック乳酸菌によるNLRP3受容体の発現増強 (2011)
  • Author(s): 遠野、麻生、北澤, ら
  • Organizer: 日本畜産学会第114回大会
  • Place of Presentation: 十和田、青森
• [Presentation] 腸管免疫能を強化するイムノバイオティック飼料添加物の開発 (2010)
  • Author(s): 北澤
  • Organizer: シンポジウム"飼料添加物をめぐる最新動向"
  • Place of Presentation: 東京
  • Year and Date: 2010-12-08
• [Presentation] イムノジェニクスとしてのオリゴヌクレオチド構造と免疫機能性 (2010)
  • Author(s): 北澤、下里
  • Organizer: 日本食品免疫学会第3回シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2010-01-18
• [Presentation] イムノジェニクスとしてのオリゴヌクレオチド構造と免疫機能性 (2010)
  • Author(s): 北澤春樹、下里剛士
  • Organizer: 日本食品免疫学会第3回シンポジウム
  • Place of Presentation: 東京大学、東京
  • Year and Date: 2010-01-18
• [Presentation] 安全な持続的家畜生産のためのイムノバイオティック革命 (2010)
  • Author(s): 北澤
  • Organizer: 第5回日本家畜臨床感染症研究会総会・学術集会
  • Place of Presentation: 東京
• [Presentation] Development of Immunobiotic Evaluation System for Functional Foods and Feeds (2010)
  • Author(s): Kitazawa, H., H. Aso, et al
  • Organizer: The 14th Animal Science Congress of the Asian-Australasian Association of Animal Production Science, Pingtung
  • Place of Presentation: Taiwan
• [Presentation] Immunobiotics as antimicrobial alternatives increase productivity in swine (2010)
  • Author(s): Suda, Y., J. Villena, H. Kitazawa, et al
  • Organizer: The 14th Animal Science Congress of the Asian-Australasian Association of Animal Production Science, Pingtung
  • Place of Presentation: Taiwan
• [Presentation] Evaluation of anti-inflammatory immunobiotics in porcine intestinal epithelial cells (2010)
  • Author(s): Kitazawa, H. H. Aso, et al
  • Organizer: International Scientific Conference on Probiotics and Prebiotics-IPC2010
  • Place of Presentation: コシチェ、スロバキア
• [Presentation] Effect of immunobiotics on the immune performance and productivity of pigs (2010)
  • Author(s): Suda, Y., J. Villena, H. Aso, H. Kitazawa, et al
  • Organizer: International Scientific Conference on Probiotics and Prebiotics-IPC2010
  • Place of Presentation: コシチェ、スロバキア
• [Presentation] 腸管上皮細胞による抗ウイルス性イムノバイオティクスの選抜・評価 (2010)
  • Author(s): 細矢、麻生、川井、齋藤、北澤ら
  • Organizer: 日本食品免疫学会第6回学術大会
  • Place of Presentation: 東京
• [Presentation] PIE細胞による抗ウイルス性イムノバイオティクスの評価系構築 (2010)
  • Author(s): 細矢、北澤ら
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京
• [Presentation] ウシ対応型の抗炎症性イムノバイオティック評価 (2010)
  • Author(s): 高梨、北澤ら
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 東京
• [Presentation] 腸管上皮(BIE)細胞株による抗炎症性イムノバイオティック評価 (2010)
  • Author(s): 高梨、麻生、川井、齋藤、北澤, ら
  • Organizer: 日本食品免疫学会第6回学術大会
  • Place of Presentation: 東京
• [Presentation] Development of Immunobiotic Evaluation System for Functional Foods and Feeds (2010)
  • Author(s): Kitazawa, H., H.Aso, et al
  • Organizer: The 14th Animal Science Congress of the Asian-Australasian Association of Animal Production Science
  • Place of Presentation: Pingtung, Taiwan
• [Presentation] Immunobiotics as antimicrobial alternatives increase productivity in swine (2010)
  • Author(s): Suda, Y., J.Villena, H.Kitazawa, et al
  • Organizer: The 14th Animal Science Congress of the Asian-Australasian Association of Animal Production Science
  • Place of Presentation: Pingtung, Taiwan
• [Presentation] PIE細胞による抗ウイルス性イムノバイオティクスの評価系構築 (2010)
  • Author(s): 細矢翔一、北澤春樹, ら
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 明治大学、東京
• [Presentation] ウシ対応型の抗炎症性イムノバイオティック評価 (2010)
  • Author(s): 高梨直也、北澤春樹, ら
  • Organizer: 日本畜産学会第112回大会
  • Place of Presentation: 明治大学、東京
• [Presentation] ブタ腸管上皮(PIE)細胞株によるイムノバイオティック飼料素材の抗炎症性評価 (2009)
  • Author(s): 細矢、北澤ら
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 那覇
• [Presentation] ウシ腸管上皮(BIE)細胞株によるイムノバイオティック評価系構築 (2009)
  • Author(s): 高梨、北澤ら
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 那覇
• [Presentation] Regulation of inflammatory response by immunobiotics in swine intestinal epithelial cells via MKP-1 (2009)
  • Author(s): Shimazu, T., H. Kitazawa, et al
  • Organizer: The 9th World Congress on Inflammation
  • Place of Presentation: Tokyo
• [Presentation] Regulation of inflammatory response by immunobiotics in swine intestinal epithelial cells via MKP-1 (2009)
  • Author(s): Shimazu, T., H.Kitazawa, et al.
  • Organizer: The 9^<th> World Congress on Inflammation
  • Place of Presentation: Keio Plaza Hotel, Tokyo
• [Presentation] ブタ腸管上皮(PIE)細胞株によるイムノバイオティック飼料素材の抗炎症性評価 (2009)
  • Author(s): 細矢翔一、北澤春樹, ら
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 琉球大学、那覇市
• [Presentation] ウシ腸管上皮(BIE)細胞株によるイムノバイオティック評価系構築 (2009)
  • Author(s): 高梨直也、北澤春樹, ら
  • Organizer: 日本畜産学会第111回大会
  • Place of Presentation: 琉球大学、那覇市
• [Book] Probiotics : Sources, Types and Health Benefits. Nova Science Publishers, Inc. (2012)
  • Author(s): Villena, J., H. Aso, S. Alvarez, H. Kitazawa
  • Publisher: (in press)
• [Book] 食品免疫・アレルギーの事典 (2011)
  • Author(s): 北澤
  • Publisher: 朝倉書店
• [Book] 乳酸菌とビフィズス菌のサイエンス (2010)
  • Author(s): 遠野、北澤
  • Publisher: 京都大学学術出版会
• [Book] Modulation of innate immunity by lactic acid bacteria, impact on host response to infections (2009)
  • Author(s): Alvarez, S., J. Villena, M. Tohno, S. Salva, H. Kitazawa
  • Publisher: Curr. Res. in Immunology
• [Patent(Industrial Property Rights)] 腸管免疫の抑制剤のスクリーニング方法 (2010)
  • Inventor(s): 北澤ら
  • Industrial Property Rights Holder: (株)明治、東北大学
  • Filing Date: 2010-05-31
• [Patent(Industrial Property Rights)] 腸管免疫の抑制剤のスクリーニング方法 (2010)
  • Inventor(s): 北澤春樹, ら
  • Industrial Property Rights Holder: 明治乳業(株)・東北大学
  • Industrial Property Number: 2010-124889
  • Filing Date: 2010-05-31