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Evaluation of Acetobacter spp. as a novel inoculant to improve silage management

Research Project

Project/Area Number 21380166
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionOkayama University

Principal Investigator

NISHINO Naoki  岡山大学, 大学院・自然科学研究科, 准教授 (50237715)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2011: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
Keywords飼料 / サイレージ / 微生物 / 畜産 / 乳酸菌 / 酢酸菌
Research Abstract

Acetobacter pasteurianus was isolated from bunker-made untreated corn silage as the predominant bacterium. The presence of A. pasteurianus was confirmed by denaturing gradient gel electrophoresis, and in other bunker-made corn silages produced in different regions including Beijing, China. Using the isolate, the effects of A. pasteurianus on fermentation, aerobic stability and bacterial community were determined. Addition of A. pasteurianus before ensiling improved aerobic stability in corn but not in grass and rice silage, whereas addition of A. pasteurianus after silo opening accelerated aerobic spoilage in corn silage. These results indicate that A. pasteurianus can inhabit bunker-made corn silage, and may show significant interactions with other bacterial species associated with anaerobic storage and aerobic deterioration.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (24 results)

All 2012 2011 2010 2009

All Journal Article (7 results) (of which Peer Reviewed: 4 results) Presentation (15 results) Book (2 results)

  • [Journal Article] Monitoring the bacterial community of maize silage stored in a bunker silo inoculated with Enterococus faecium, Lactobacillus plantarum and Lactobacillus buchneri2011

    • Author(s)
      Li, Y. and Nishino, N.
    • Journal Title

      Journal of Applied Microbiology

      Volume: 110 Pages: 1561-1570

    • Related Report
      2011 Final Research Report
  • [Journal Article] Bacterial and fungal communities of wilted Italian ryegrass silage inoculated with and without Lactobacillus rhamnosus or Lactobacillus buchneri2011

    • Author(s)
      Li, Y. and Nishino, N.
    • Journal Title

      Letters in Applied Microbiology

      Volume: 52 Pages: 314-321

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Journal Article] Effects of inoculation of Lactobacillus rhamnosus and Lactobacillus buchneri on fermentation, aerobic stability and microbial communities in whole crop corn silage.2011

    • Author(s)
      Li, Y. and Nishino, N.
    • Journal Title

      Grassland Science

      Volume: 57 Pages: 184-191

    • NAID

      10030565024

    • Related Report
      2011 Final Research Report
  • [Journal Article] Monitoring the bacterial community of maize silage stored in a bunker silo inoculated with Enterococcus faecium, Lactobacillus plantarum and Lactobacillus buchneri2011

    • Author(s)
      Li, Y., Nishino, N.
    • Journal Title

      Journal of Applied Microbiology

      Volume: 110 Issue: 6 Pages: 1561-1570

    • DOI

      10.1111/j.1365-2672.2011.05010.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of inoculation of Lactobacillus rhamnosus and Lactobacillus buchnerion fermentation, aerobic stability and microbial communities in whole crop corn silage2011

    • Author(s)
      Li, Y., Nishino, N.
    • Journal Title

      Grassland Science

      Volume: 57 Issue: 4 Pages: 184-191

    • DOI

      10.1111/j.1744-697x.2011.00226.x

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Monitoring the bacterial community of maize silage stored in a bunker silo inoculated with Enterococcus faecium, Lactobacillus plaotarum and Lactobacillus buchneri.2011

    • Author(s)
      Li, Y., Nishino, N.
    • Journal Title

      Journal of Applied Microbiology

      Volume: 110 Pages: 1561-1570

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of inoculation of Lactobacillus rhamnosus and Lactobacillus buchneri on fermentation, aerobic stability and microbial communities in whole crop corn silage2011

    • Author(s)
      Li, Y., Nishino, N.
    • Journal Title

      Grassland Science

      Volume: (印刷中)

    • NAID

      10030565024

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Ensiling fermentation, aerobic stability and bacterial community of direct-cut and wilted alfalfa silage with and without addition of Lactobacillus rhamnosus and molasses2012

    • Author(s)
      Wu, B., Cui, Y. and Nishino, N.
    • Organizer
      Proceedings of the 4th Japan-China-Korea Grassland Conference
    • Place of Presentation
      Aichi
    • Year and Date
      2012-03-31
    • Related Report
      2011 Final Research Report
  • [Presentation] アルファルファサイレージの発酵特性と好気的安定性に関わる細菌フローラ2012

    • Author(s)
      呉白乙拉, 西野直樹
    • Organizer
      日本畜産学会第114回大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2012-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of inoculation of Acetobacter pasteurianus at ensiling and after silo opening on fermentation products, aerobic stability, and bacterial community of whole crop corn silage2011

    • Author(s)
      Han, H. and Nishino, N.
    • Organizer
      Proceedings of the 2nd International Symposium on Forage Quality and Conservation
    • Place of Presentation
      Sao Pedro
    • Year and Date
      2011-11-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of inoculation of Acetobacter pasteurianus at ensiling and after silo opening on fermentation products, aerobic stability, and bacterial community of whole crop corn silage2011

    • Author(s)
      Han, H., Nishino, N.
    • Organizer
      2nd International Symposium on Forage Quality and Conservation
    • Place of Presentation
      Sao Pedro (Brazil)
    • Year and Date
      2011-11-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Aerobic stability and instability of silages caused by bacteria2011

    • Author(s)
      Nishino, N.
    • Organizer
      2nd International Symposium on Forage Quality and Conservation
    • Place of Presentation
      Sao Pedro (Brazil)(招待講演)
    • Year and Date
      2011-11-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Non-plate methods for silage microorganisms identification2011

    • Author(s)
      Nishino, N.
    • Organizer
      2nd International Symposium on Forage Quality and Conservation
    • Place of Presentation
      Sao Pedro (Brazil)(招待講演)
    • Year and Date
      2011-11-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] トウモロコシサイレージの貯蔵および好気的変敗におけるAcetobacter pasteurianusの役割2011

    • Author(s)
      韓紅燕, 西野直樹
    • Organizer
      関西畜産学会第61回大会
    • Place of Presentation
      岡山市
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] イタリアンライグラスおよびギニアグラスから調製したダイレクトカットおよび予乾サイレージの発酵特性と細菌フローラ2011

    • Author(s)
      李雁氷, 西野直樹
    • Organizer
      日本草地学会第67回発表会
    • Place of Presentation
      宇都宮市
    • Year and Date
      2011-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] コントラクタが調製したトウモロコシサイレージの細菌群集2010

    • Author(s)
      李雁氷, 西野直樹
    • Organizer
      日本畜産学会第112回大会
    • Place of Presentation
      千代田区
    • Year and Date
      2010-03-29
    • Related Report
      2011 Final Research Report
  • [Presentation] コントラクタが調製したトウモロコシサイレージの細菌群集2010

    • Author(s)
      李雁氷・西野直樹
    • Organizer
      日本畜産学会第112回大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2009 Annual Research Report
  • [Presentation] イタリアンライグラスサイレージの貯蔵および好気的変敗過程における微生物フローラの変化2010

    • Author(s)
      李雁氷, 西野直樹
    • Organizer
      日本草地学会第66回発表会
    • Place of Presentation
      津市
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report
  • [Presentation] トウモロコシサイレージの好気的変敗に対するAcetobacter pasteurianusの添加効果2010

    • Author(s)
      韓紅燕, 西野直樹
    • Organizer
      日本草地学会第66回発表会
    • Place of Presentation
      津市
    • Year and Date
      2010-03-26
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] バンカーサイロから分離したAcetobacter pasteurianusの性質とトウモロコシサイレージにおける生残性2009

    • Author(s)
      西野直樹, 韓紅燕, 王超, 李雁氷, Parvin, S.
    • Organizer
      関西畜産学会第59回大会
    • Place of Presentation
      鳥取市
    • Year and Date
      2009-08-27
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Occurrence and survival in whole crop corn silage of Acetobacter pasteurianus2009

    • Author(s)
      Nishino, N., Wang, C., Li, Y., Parvin, S. and Han, H.
    • Organizer
      Proceedings of the 15th International Silage Conference
    • Place of Presentation
      Wisconsin
    • Year and Date
      2009-07-29
    • Related Report
      2011 Final Research Report
  • [Presentation] Variations in bacterial community and survival of inoculated bacteria in bunker silos assessed by denaturing gradient gel electrophoresis profiles2009

    • Author(s)
      Li, Y. and Nishino, N.
    • Organizer
      Proceedings of the 15th International Silage Conference
    • Place of Presentation
      Wisconsin
    • Year and Date
      2009-07-29
    • Related Report
      2011 Final Research Report
  • [Book] Aerobic Stability and Instability of Silages Caused by Bacteria. in Proceedings of the 2nd International Symposium on Forage Quality and Conservation(Daniel, J. L. P., Zopollatto, M., and Nussio, L. G. eds.)2011

    • Author(s)
      Nishino, N.
    • Publisher
      Piracicaba, Brazil
    • Related Report
      2011 Final Research Report
  • [Book] サイレージ発酵の原理最新サイレージバイブル-サイレージとTMRの調製と給与-(安宅一夫監修)2011

    • Author(s)
      西野直樹
    • Publisher
      酪農大学エクステンションセンター
    • Related Report
      2011 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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