• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of tolerance induction method using clone pigs remodeled for expressing human ABO blood group antigen

Research Project

Project/Area Number 21380168
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionObihiro University of Agriculture and Veterinary Medicine

Principal Investigator

OGAWA Haruko  帯広畜産大学, 動物・食品衛生研究センター, 准教授 (10400079)

Co-Investigator(Kenkyū-buntansha) OHNISHI Akira  独立行政法人農業生物資源研究所, 医用モデルブタ研究開発ユニット, 上級研究員 (30414890)
KOBAYASHI Takaaki  名古屋大学, 大学院・医学系研究科, 寄附講座教授 (70314010)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥15,080,000 (Direct Cost: ¥11,600,000、Indirect Cost: ¥3,480,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Keywordsクローン家畜 / 核移植 / 移植 / 拒絶反応 / 免疫寛容 / ABO式血液型抗原 / 移植・再生医療 / 免疫学 / 糖鎖 / バイオテクノロジー / 血液型抗原 / クローン動物 / リンパ球 / ABO
Research Abstract

This study aims to generate transgenic pigs expressing human ABO blood group antigens by using nuclear transfer method, and utilize the pigs to develop a novel tolerance induction method to control the rejection in ABO-incompatible transplantation. First, transgenic pig carrying one of the two glycosyltransferase genes to be transferred was produced, and then the second glycosyltransferase gene was transferred to the somatic cells of the transgenic pig. Using the genetically-engineered cells as a donor cell for nuclear transfer, purposed transgenic pig was produced. There has been seen some problems in the growth after birth and the expression of the purposed antigen, therefore additional studies are ongoing to solve the problems.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (34 results)

All 2012 2011 2010 2009

All Journal Article (12 results) (of which Peer Reviewed: 11 results) Presentation (19 results) Book (3 results)

  • [Journal Article] Comparative study on signal transduction in endothelial cells after anti-A/B and HLA antibody reaction : implication of accommodation2012

    • Author(s)
      Iwasaki K, Miwa Y, Ogawa H, Iwamoto M, Furusawa T, Onishi A, Kuzuya T, Haneda M, Watarai Y, Uchida K, Kobayashi T.
    • Journal Title

      Transplantation

      Volume: 93 Issue: 4 Pages: 390-397

    • DOI

      10.1097/tp.0b013e3182424df3

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Production of cloned pigs expressing human thrombomodulin in endothelial cells2012

    • Author(s)
      Yazaki S, Iwamoto M, Onishi A, Miwa Y, Hashimoto M, Oishi T, Suzuki S, Fuchimoto D, Sembon S, Furusawa T, Liu D, Nagasaka T, Kuzuya T, Ogawa H, Yamamoto K, Iwasaki K, Haneda M, Maruyama S, Kobayashi T.
    • Journal Title

      Xenotransplantation

      Volume: 19 Issue: 2 Pages: 82-91

    • DOI

      10.1111/j.1399-3089.2012.00696.x

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potent immunosuppression for ABO-incompatible renal transplantation may not be a risk factor for malignancy2012

    • Author(s)
      Yamamoto T, Kawaguchi T, Watarai Y, Tujita M, Hiramitsu T, Nanmoku K, Goto N, Katayama A, Kobayashi T, Uchida K.
    • Journal Title

      Transplant Proc.

      Volume: 44 Issue: 1 Pages: 210-213

    • DOI

      10.1016/j.transproceed.2011.11.048

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparative study on signal transduction in endothelial cells after anti-A/B and human leukocyte antigen antibody reaction : implication of accommodation2012

    • Author(s)
      Iwasaki K., Miwa Y., Ogawa H., et al.
    • Journal Title

      Transplantation

      Volume: 93 Pages: 390-397

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Potent immunosuppression for ABO-incompatible renal transplantation may not be a risk factor for malignancy2012

    • Author(s)
      Yamamoto T., Kawaguchi T., Watarai Y., wt al.
    • Journal Title

      Transplant Proc

      Volume: 44 Pages: 210-213

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ABO血液型糖鎖抗原の切断2011

    • Author(s)
      小林孝彰
    • Journal Title

      Organ Biology

      Volume: 18 Pages: 116-120

    • NAID

      130004709993

    • URL

      http://mol.medicalonline.jp/archive/select?jo=ca3organ

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Surveillance of avian influenza virus in migratory water birds in eastern Hokkaido, Japan.2011

    • Author(s)
      Bui VN
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 73 Pages: 209-215

    • Related Report
      2010 Annual Research Report
  • [Journal Article] Potential value of human thrombomodulin and DAF expression for coagulation control in pig-to-human xenotransplantation2010

    • Author(s)
      Miwa Y, Yamamoto K, Onishi A, Iwamoto M, Yazaki S, Haneda M, Iwasaki K, Liu D, Ogawa H, Nagasaka T, Uchida K, Nakao A, Kadomatsu K, Kobayashi T.
    • Journal Title

      Xenotransplantation

      Volume: 17 Issue: 1 Pages: 26-37

    • DOI

      10.1111/j.1399-3089.2009.00555.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Potential value of human thrombomodulin and DAF expression for coagulation control in pig-to-human xenotransplantation.2010

    • Author(s)
      Miwa Y
    • Journal Title

      Xenotransplantation. 17

      Pages: 26-37

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successful cross-breeding of cloned pigs expressing endo-galactosidase C and hDAF2009

    • Author(s)
      Yazaki S, Iwamoto M, Onishi A, Miwa Y, Suzuki S, Fuchimoto D, Sembon S, Furusawa T, Hashimoto M, Oishi T, Liu D, Nagasaka T, Kuzuya T, Maruyama S, Ogawa H, Kadomatsu K, Uchida K, Nakao A, Kobayashi T.
    • Journal Title

      Xenotransplantation

      Volume: 16 Issue: 6 Pages: 511-521

    • DOI

      10.1111/j.1399-3089.2009.00549.x

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Removal of blood group A/B antigen in organs by ex vivo and in vivo administration of endo-galactosidase (ABase) for ABO-incompatible transplantation2009

    • Author(s)
      Kobayashi T, Liu D, Ogawa H, Miwa Y, Nagasaka T, Maruyama S, Li Y-T, Onishi A, Iwamoto M, Kuzuya T, Kadomatsu K, Uchida K, Nakao A.
    • Journal Title

      Transpl. Immunol

      Volume: 20 Pages: 132-138

    • URL

      http://dx.doi.org/10.1016/j.trim.2008.09.007

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Successful cross-breeding of clonal pigs expressing endo-b-galactosisdase C(EndoGalC)and human decay accelerating factor(hDAF).2009

    • Author(s)
      Yazaki S
    • Journal Title

      Xenotransplantation. 16

      Pages: 511-521

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Successful production and breeding of cloned pigs expressing human thrombomodulin in endothelial cells2011

    • Author(s)
      Iwamoto M, Yazaki S, Onishi A, Miwa Y, Hashimoto M, Oishi T, Suzuki S, Fuchimoto D, Sembon S, Nagasaka T, Liu D, Iwasaki K, Haneda M, Yamamoto K, Maruyama S, Kobayashi T.
    • Organizer
      CTS-IXA 2011 Joint Congress
    • Place of Presentation
      Miami (USA)
    • Related Report
      2011 Final Research Report
  • [Presentation] Difference in signal transduction between anti-A/B and anti-HLA antibody binding to endothelial cells2011

    • Author(s)
      Iwasaki K, Miwa Y, Haneda M, Kobayashi T.
    • Organizer
      CTS-IXA 2011 Joint Congress
    • Place of Presentation
      Miami (USA)
    • Related Report
      2011 Final Research Report
  • [Presentation] Complement and thrombin block endothelial cell accommodation2011

    • Author(s)
      Iwasaki K, Miwa Y, Haneda M, Kobayashi T.
    • Organizer
      CTS-IXA 2011 Joint Congress
    • Place of Presentation
      Miami (USA)
    • Related Report
      2011 Final Research Report
  • [Presentation] 異種移植における凝固系制御の試み:ヒトトロンボモジュリン遺伝子導入クローンブタの作出2011

    • Author(s)
      岩元正樹,矢崎智子,三輪祐子,丸山彰一,鈴木俊一,橋本径子,大石貴嗣,劉大革,長坂隆治,小林孝彰,大西彰
    • Organizer
      第47回日本移植学会総会
    • Place of Presentation
      仙台
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] ERK活性化細胞外因子はAccommodation誘導を阻害する2011

    • Author(s)
      岩崎研太,三輪祐子,羽根田正隆,矢崎智子,岩元正樹,大西彰,小川晴子,小林孝彰
    • Organizer
      第47回日本移植学会総会
    • Place of Presentation
      仙台
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] human thrombomodulin高発現体細胞クローンブタおよびその後代の作出2011

    • Author(s)
      岩元正樹,矢崎智子,三輪祐子,山本晃士,丸山彰一,鈴木俊一,淵本大一郎,千本正一郎,鈴木美佐枝,橋本径子,大石貴嗣,羽根田正隆,岩崎研太,中尾昭公,小林孝彰,大西彰
    • Organizer
      第16回日本病態プロテアーゼ学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Successful production and breeding of cloned pigs expressing human thrombomodulin in endothelial cells2011

    • Author(s)
      Iwamoto M., Yazaki S., Onishi A., et al.
    • Organizer
      CTS-IXA 2011 Joint Congress (oral)
    • Place of Presentation
      Miami (USA)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Difference in signal transduction between anti-A/B and anti-HLA antibody binding to endothelial cells2011

    • Author(s)
      Iwasaki K., Miwa Y., Haneda M., et al.
    • Organizer
      CTS-IXA 2011 Joint Congress (oral)
    • Place of Presentation
      Miami (USA)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Complement and thrombin block endothelial cell accommodation2011

    • Author(s)
      Iwasaki K., Miwa Y., Haneda M., et al.
    • Organizer
      CTS-IXA 2011 Joint Congress (oral)
    • Place of Presentation
      Miami (USA)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Functional difference between membrane-bound and soluble human thrombomodulin2011

    • Author(s)
      Miwa Y., Yazaki S., Iwamoto M., et al.
    • Organizer
      CTS-IXA 2011 Joint Congress (oral)
    • Place of Presentation
      Miami (USA)
    • Related Report
      2011 Annual Research Report
  • [Presentation] 異種移植におけるhuman thrombomodulinの有用性2011

    • Author(s)
      三輪祐子, 矢崎智子, 岩元正樹, 他
    • Organizer
      第16回日本病態プロテアーゼ学会
    • Place of Presentation
      大阪
    • Related Report
      2011 Annual Research Report
  • [Presentation] 異種移植における可溶性および膜型ヒトトロンボモジュリンの比較検討2011

    • Author(s)
      三輪祐子, 矢崎智子, 岩元正樹, 他
    • Organizer
      第47回日本移植学会総会
    • Place of Presentation
      仙台
    • Related Report
      2011 Annual Research Report
  • [Presentation] ABO不適合移植における抗体接着が誘導する細胞保護に関する研究2010

    • Author(s)
      岩崎研太,三輪祐子,羽根田正隆,矢崎智子,岩本正樹,大西彰,小川晴子,小林孝彰
    • Organizer
      第46回日本移植学会総会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] The reduction of RAF/MEK/ERK pathway by anti-ABO association may be the identity of accommodation in ABO-incompatible transplantation2010

    • Author(s)
      Iwasaki K, Miwa Y, Haneda M, Ogawa H, Uchida K, Takeda A, Nakao A, Kobayashi T.
    • Organizer
      23rd International Congress of The Transplantation Society
    • Place of Presentation
      Vancouver (Canada)
    • Related Report
      2011 Final Research Report
  • [Presentation] Effective suppression of direct prothrombinase activity in porcine endothelial cells by human thrombomodulin (hTM) gene transfer or its soluble form2010

    • Author(s)
      Miwa Y, Yazaki S, Iwamoto M, Onishi A, Yamamoto K, Iwasaki K, Haneda M, Maruyama S, Kadomatsu K, Uchida K, Kobayashi T.
    • Organizer
      American Transplant Congress 2010
    • Place of Presentation
      San Diego (USA)
    • Related Report
      2011 Final Research Report
  • [Presentation] ABO式血液型抗原に対する免疫寛容の誘導に関する研究2009

    • Author(s)
      小川晴子
    • Organizer
      第45回日本移植学会総会
    • Place of Presentation
      京王プラザホテル(東京)
    • Year and Date
      2009-09-17
    • Related Report
      2009 Annual Research Report
  • [Presentation] Human thrombomodulin plays an important role in coagulaiton control : engine braking is essential for physiological regulation2009

    • Author(s)
      Miwa Y, Iwasaki K, Yamamoto K, Maruyama S, Onishi A, Iwamoto M, Yazaki S, Liu DG, Nagasaka T, Haneda M, Kadomatsu K, Kobayashi T.
    • Organizer
      IPITA-IXA 2009
    • Place of Presentation
      Venice (Italy)
    • Related Report
      2011 Final Research Report
  • [Presentation] ABO式血液型抗原に対する免疫寛容の誘導に関する研究2009

    • Author(s)
      小川晴子,大西彰,岩元正樹,羽根田正隆,岩崎研太,三輪祐子,小林孝彰
    • Organizer
      (口演)第45回日本移植学会総会
    • Place of Presentation
      東京
    • Related Report
      2011 Final Research Report
  • [Presentation] Potential impacts of human DAF and thrombomodulin on thrombin regulation2009

    • Author(s)
      Miwa Y, Yamamoto K, Onishi A, Iwamoto M, Maruyama S, Nakao A, Kadomatsu K, Kobayashi T.
    • Organizer
      American transplant congress 2009
    • Place of Presentation
      Boston (USA)
    • Related Report
      2011 Final Research Report
  • [Book] 異種移植研究から臨床へ:遺伝子組換えブタに対する期待と日本の役割2010

    • Author(s)
      小林孝彰
    • Publisher
      Techno Innovation
    • Related Report
      2011 Final Research Report
  • [Book] 腎移植における免疫モニタリングの試み.今日の移植2009

    • Author(s)
      小林孝彰
    • Related Report
      2011 Final Research Report
  • [Book] ABO血液型抗体の測定腎移植のすべて2009

    • Author(s)
      小林孝彰
    • Publisher
      メジカルレビュー社
    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi