| Project/Area Number |
21380170
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Tohoku University |
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Principal Investigator |
ASO Hisashi 東北大学, 大学院・農学研究科, 教授 (50241625)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Takahiro 東北大学, 大学院・農学研究科, 教授 (20111297)
OHWADA Shuichi 東北大学, 大学院・農学研究科, 助教 (00183244)
WATANABE Koichi 東北大学, 大学院・農学研究科, 助教 (80261494)
KITAZAWA Haruki 東北大学, 大学院・農学研究科, 准教授 (10204885)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | 機能性物質 / M細胞 / プリオン蛋白 / トランスサイトーシス / 膜蛋白 / 輸送小胞体 / aldolase A / 親和性 / Far western blot解析 / 磁気ビーズ / プロテオミクス解析 / 2次元電気泳動 |
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| Research Abstract |
In order to identify a membrane protein associated with the Prion invasion in bovine intestinal microfold (M)cells, M cell-differentiated bovine intestinal epitheliocyte cell line (BIE cells)were treated with recombinant bovine prion protein-coated magnetic beads, and then vesicles including magnetic beads were purified from BIE cells. After 2D-electrophoresis, fructose-bisphosphate aldolase A was identified using LC-MS/MS spectrometer. Aldolase A were specifically expressed on M cells in ileal Peyer's patches and the epithelium in myctero pharynges, these tissues of which were thought to be associated with Prion invasion. In addition, the far western analysis revealed that aldolase A could bind to prion protein.
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