Identification of membrane proteins associated with Prion invasion in bovine intestinal microfold (M)cells
Project/Area Number |
21380170
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied animal science
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Research Institution | Tohoku University |
Principal Investigator |
ASO Hisashi 東北大学, 大学院・農学研究科, 教授 (50241625)
|
Co-Investigator(Kenkyū-buntansha) |
YAMAGUCHI Takahiro 東北大学, 大学院・農学研究科, 教授 (20111297)
OHWADA Shuichi 東北大学, 大学院・農学研究科, 助教 (00183244)
WATANABE Koichi 東北大学, 大学院・農学研究科, 助教 (80261494)
KITAZAWA Haruki 東北大学, 大学院・農学研究科, 准教授 (10204885)
|
Project Period (FY) |
2009 – 2011
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Project Status |
Completed (Fiscal Year 2011)
|
Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
|
Keywords | 機能性物質 / M細胞 / プリオン蛋白 / トランスサイトーシス / 膜蛋白 / 輸送小胞体 / aldolase A / 親和性 / Far western blot解析 / 磁気ビーズ / プロテオミクス解析 / 2次元電気泳動 |
Research Abstract |
In order to identify a membrane protein associated with the Prion invasion in bovine intestinal microfold (M)cells, M cell-differentiated bovine intestinal epitheliocyte cell line (BIE cells)were treated with recombinant bovine prion protein-coated magnetic beads, and then vesicles including magnetic beads were purified from BIE cells. After 2D-electrophoresis, fructose-bisphosphate aldolase A was identified using LC-MS/MS spectrometer. Aldolase A were specifically expressed on M cells in ileal Peyer's patches and the epithelium in myctero pharynges, these tissues of which were thought to be associated with Prion invasion. In addition, the far western analysis revealed that aldolase A could bind to prion protein.
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Report
(4 results)
Research Products
(84 results)
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[Journal Article] Immunobiotic Lactobacillus jensenii elicit anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the toll-like receptor signaling pathway.2012
Author(s)
Tomoyuki Shimazu, Julio Villena, Masanori Tohno, Hitomi Fujie, Shoichi Hosoya, Takeshi Shimosato, Hisashi Aso, Yoshihito Suda, Yasushi Kawai, Tadao Saito, Seiya Makino, Shuji Ikegami, Hiroyuki Itoh, Haruki Kitazawa.
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Journal Title
Infection and Immunity
Volume: 80 (1)
Pages: 276-288
Related Report
Peer Reviewed
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[Journal Article] Orally administered prion protein is incorporated by M cells and propagated into lymphoid tissues by macrophages in prion protein knockout mice.2011
Author(s)
Ikuro Takakura, Kohtaro Miyazawa, Takashi Kanaya, Wataru Itani, Kouichi Watanabe, Shyuichi Ohwada, Hitoshi Watanabe, Michael T. Rose, Suehiro Sakaguchi, Noriyuki Nishida, Shigeru Katamine, Takahiro Yamaguchi, Hisashi Aso.
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Journal Title
American Journal of Pathology
Volume: 179(3)
Pages: 1301-1309
Related Report
Peer Reviewed
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[Journal Article] Peripheral serotonin enhances lipid metabolism by accelerating bile acid turnover.2010
Author(s)
Hitoshi Watanabe, Daisuke Akasaka, Hideki Ogasawara, Kan Sato, Masato Miyake, Kazuki Saito, Takashi Kanaya, Ikuro Takakura, Guozheng Chao, Michael T. Rose, Shyuichi Ohwada, Kouichi Watanabe, Takahiro Yamaguchi, Hisashi Aso.
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Journal Title
Endocrinology
Volume: 151(10)
Pages: 4776-4786
Related Report
Peer Reviewed
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[Journal Article] Transcytosis of murine-adapted BSE agent in an in vitro bovine M cell model.2010
Author(s)
Kohtaro Miyazawa, Takashi Kanaya, Ikuro Takakura, Sachi Tanaka, Tetsuya Hondo, Hitoshi Watanabe, Michael T. Rose, Haruki Kitazawa, Takahiro Yamaguchi, Shigeru Katamine, Noriyuki Nishida, Hisashi Aso.
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Journal Title
Journal of Virology
Volume: 84(23)
Pages: 12285-12291
Related Report
Peer Reviewed
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[Presentation] Evaluation of anti-inflammatory immunobiotics in porcine intestinal epithelial cell2010
Author(s)
Kitazawa, Haruki ; Shimazu, Tomoyuki ; Tohno, Masanori ; Villena, Julio ; Hosoya, Syoichi ; Fujie, Hitomi ; Suda, Yoshihito ; Shimosato, Takeshi ; Aso, Hisashi ; Makino, Seiya ; Ikegami, Shuuji ; Kawai, Yasushi ; Saito, Tadao
Organizer
International Scientific Conference on Probiotics and Prebiotics-IPC2010
Place of Presentation
Kosice, Slovakia
Related Report
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[Presentation] Effect of immunobiotics on the immune performance and productivity of pigs2010
Author(s)
Suda, Yoshihito ; Takahashi, Yu ; Tohno, Masanori ; Villena, Julio ; Hosoya, Syoichi ; Fujie, Hitomi ; Shimosato, Takeshi ; Kobashi, Jin ; Ishida, Mitsuharu ; Aso, Hisashi ; Kawai, Yasushi ; Saito, Tadao ; Makino, Seiya ; Ikegami, Shuuji ; Kitazawa, Haruk
Organizer
International Scientific Conference on Probiotics and Prebiotics-IPC2010
Place of Presentation
Kosice, Slovakia
Related Report
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[Presentation] Peripheral Serotonin Enhanced Bile Acids Circulation and Accelerated Lipid Metabolism2009
Author(s)
H. Watanabe, D. Akasaka, K. Saito, H. Ogasawara, T. Kanaya, M. Miyake, S. Iwasaki, T. Hondo, Y. Takahashi, K. Sato, S. Ohwada, K. Watanabe, T. Yamaguti, H. Aso
Organizer
49th Annual Meeting, The American Society for Cell Biology
Place of Presentation
San Diego, U. S. A
Related Report
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[Presentation] Cytokeratin 18 Is a Specific Marker of M Cell of Bovine Peyer's Patches2009
Author(s)
T. Hondo, H. Aso, T. Kanaya, I. Takakura, H. Watanabe, M. Miyake, S. Iwasaki, K. Saito, Y. Takahashi, S. Ohwada1, K. Watanabe, T. Yamaguti
Organizer
49th Annual Meeting, The American Society for Cell Biology
Place of Presentation
San Diego, U. S. A
Related Report
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