Regeneration ofthe chick organs and the application for breeding by use of a stem cell
| Project/Area Number |
21380173
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Shinshu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ONO Tamao 信州大学, 農学部, 教授 (10177264)
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| Project Period (FY) |
2009 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
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| Keywords | 育種 / 胚性幹細胞 / 体性幹細胞 / 臓器 / 器官 / 再生 / 家禽 / 幹細胞 / 分化制御 / 臓器再生 / 個体再生 / sp細胞 / 単一幹細胞 / SP細胞 / 個体再 |
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| Research Abstract |
Stem cells which retained pluripotency was isolated from the chick embryos or somatic cells. The obtained stem cells were used as donor cell for making germline and somatic chicmeras. The embryonic stem cells were derived from area pellucida of stage X blastoderm. The somatic stem cells were obtained from bone marrowcells or adipocytes. The germline chimeras were effectively generated by the embryonic stem cells. The bone marrow cells or adipocyte could be regenerated into vein, muscle or bone cells. Due to these results, the stem cells could be useful tools for future poultry breeding.
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Report
(5 results)
Research Products
(38 results)
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[Journal Article] X-irradiation Removes Endogenous Primordial Germ Cells (PGCs) and Increases Germline Transmission of Donor PGCs in Chimeric Chickens2012
Author(s)
Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Ono, T., Kagami, H., Takeda, K., Nirasawa, K., Tagami, T
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Journal Title
Journal of Reproduction and Development
Volume: 58
Pages: 432-437
NAID
Related Report
Peer Reviewed
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[Journal Article] Efficient System for Preservation and Regeneration of Genetic Resources in Chicken: Concurrent Store of Primordial Germ Cells and Live Animals from early Embryos of A Rare Indigenous Fowl (Gifujidoro). Reproduction2010
Author(s)
Nakamura, Y., Usui, F., Miyahara, D., Mori, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H and Tagami, T.
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Journal Title
Fertility and Development
Volume: 22
Pages: 1237-1344
Related Report
Peer Reviewed
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[Journal Article] Germline Replacement by Transfer of Primordial Germ Cells into Partially Sterilized Embryos in the Chicken.2010
Author(s)
Nakamura, Y., Usui, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H and Tagami, T.
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Journal Title
Biology of Reproduction
Volume: 83
Pages: 130-137
Related Report
Peer Reviewed
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[Journal Article] A Novel Concentration System of Chicken Stem Cells by Bone Marrow Side Population Cells.2010
Author(s)
Usui, F., Nakamura, Y., Yamamoto, Y., Tatsumi, K., Tominari, K., Ono, T. and Kagami, H
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Journal Title
Journal of Poultry Science
Volume: 47
Pages: 53-56
NAID
Related Report
Peer Reviewed
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[Presentation] Effect ofX-ray Irradiation of Primordoal Germ Cell-depletion in Chicken Embryos.2011
Author(s)
Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Watanabe, H., Ono, T., Takeda, K., Nirasawa, Kagami, H., Tagami, T
Organizer
th Asia Pacific Poultry Conference
Place of Presentation
Taipei
Year and Date
2011-03-22
Related Report
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[Presentation] Avian Stem Cells and the Application for Biotechnology.2010
Author(s)
Kagami, H., Usui, F., Nakamura, Y., Kashiwagi, M., Yamamoto, Y., Ono, T., Tagami, T.,Nirasawa, K., Matsubara, Y., Petitte, J.N.
Organizer
43rd Annual Meeting for the Japanese Society of Developmental Biologist
Place of Presentation
Kyoto
Year and Date
2010-06-21
Related Report
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