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Regeneration ofthe chick organs and the application for breeding by use of a stem cell

Research Project

Project/Area Number 21380173
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied animal science
Research InstitutionShinshu University

Principal Investigator

KAGAMI Hiroshi  信州大学, 農学部, 教授 (80308303)

Co-Investigator(Kenkyū-buntansha) ONO Tamao  信州大学, 農学部, 教授 (10177264)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥19,500,000 (Direct Cost: ¥15,000,000、Indirect Cost: ¥4,500,000)
Fiscal Year 2012: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2011: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2010: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2009: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywords育種 / 胚性幹細胞 / 体性幹細胞 / 臓器 / 器官 / 再生 / 家禽 / 幹細胞 / 分化制御 / 臓器再生 / 個体再生 / sp細胞 / 単一幹細胞 / SP細胞 / 個体再
Research Abstract

Stem cells which retained pluripotency was isolated from the chick embryos or somatic cells. The obtained stem cells were used as donor cell for making germline and somatic chicmeras. The embryonic stem cells were derived from area pellucida of stage X blastoderm. The somatic stem cells were obtained from bone marrowcells or adipocytes. The germline chimeras were effectively generated by the embryonic stem cells. The bone marrow cells or adipocyte could be regenerated into vein, muscle or bone cells. Due to these results, the stem cells could be useful tools for future poultry breeding.

Report

(5 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (38 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (10 results) (of which Peer Reviewed: 9 results) Presentation (24 results) (of which Invited: 2 results) Book (3 results) Remarks (1 results)

  • [Journal Article] Development, Differentiation and Manipulation of Chicken Germ Cells2013

    • Author(s)
      Nakamura, Y., Kagami, H., Tagami, T
    • Journal Title

      Development, Growth & Differentiation

      Volume: 55 Pages: 20-40

    • NAID

      40019549269

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development, differentiation and manipulation of chicken germ cells2013

    • Author(s)
      Yoshiaki Nakamura, Hiroshi Kagami, Takahiro Tagami
    • Journal Title

      Development, Growth & Differentiation

      Volume: 55 Pages: 20-40

    • NAID

      40019549269

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] X-irradiation Removes Endogenous Primordial Germ Cells (PGCs) and Increases Germline Transmission of Donor PGCs in Chimeric Chickens2012

    • Author(s)
      Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Ono, T., Kagami, H., Takeda, K., Nirasawa, K., Tagami, T
    • Journal Title

      Journal of Reproduction and Development

      Volume: 58 Pages: 432-437

    • NAID

      10031066902

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] X-irradiation Removes Endogenous Primordial Germ Cells and Increas Germline Transmission of Donor PGCs in Chimeric Chickens2012

    • Author(s)
      Hiroshi Kagami
    • Journal Title

      Journal of Reproduction and Development

      Volume: (掲載確定)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Simple Culture method of Chicken Blastodermal Cells for Germline Transmission2011

    • Author(s)
      Matsubara, Y., Hirota, A., Sobajima, H., Kagami, H., Tagami, T. and Yasue. H.
    • Journal Title

      Journal of Poultry Science

      Volume: 48 Pages: 14-18

    • NAID

      10027817484

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A Simple Culture Method of Chicken Blastodermal Cells for Germline Transmission.2011

    • Author(s)
      Hiroshi Kagami
    • Journal Title

      Journal of Poultry Science

      Volume: 48 Pages: 14-18

    • NAID

      10027817484

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Efficient System for Preservation and Regeneration of Genetic Resources in Chicken: Concurrent Store of Primordial Germ Cells and Live Animals from early Embryos of A Rare Indigenous Fowl (Gifujidoro). Reproduction2010

    • Author(s)
      Nakamura, Y., Usui, F., Miyahara, D., Mori, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H and Tagami, T.
    • Journal Title

      Fertility and Development

      Volume: 22 Pages: 1237-1344

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Germline Replacement by Transfer of Primordial Germ Cells into Partially Sterilized Embryos in the Chicken.2010

    • Author(s)
      Nakamura, Y., Usui, F., Ono, T., Takeda, K., Nirasawa, K., Kagami, H and Tagami, T.
    • Journal Title

      Biology of Reproduction

      Volume: 83 Pages: 130-137

    • Related Report
      2012 Final Research Report 2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Novel Concentration System of Chicken Stem Cells by Bone Marrow Side Population Cells.2010

    • Author(s)
      Usui, F., Nakamura, Y., Yamamoto, Y., Tatsumi, K., Tominari, K., Ono, T. and Kagami, H
    • Journal Title

      Journal of Poultry Science

      Volume: 47 Pages: 53-56

    • NAID

      10026253103

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel concentrating system of chicken stem cells by bone marrow side population cells2010

    • Author(s)
      Hiroshi Kagami
    • Journal Title

      The Journal of Poultry Science 47

      Pages: 53-56

    • NAID

      10026253103

    • Related Report
      2009 Annual Research Report
  • [Presentation] 幹細胞の畜産への活用2013

    • Author(s)
      鏡味裕
    • Organizer
      日本畜産学会第116回大会
    • Place of Presentation
      広島
    • Year and Date
      2013-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] Future Possibility and Current Status of AnimalStem Cell Technology2012

    • Author(s)
      Kagami, H.
    • Organizer
      The 2ndJapan-Korea-China Joint Symposium
    • Place of Presentation
      Society for Reproduction, Tsukuba
    • Year and Date
      2012-09-05
    • Related Report
      2012 Final Research Report
  • [Presentation] Perspective for Animal Biotechnology.2012

    • Author(s)
      Kagami, H.
    • Organizer
      International Symposium on Animal Biotechnology
    • Place of Presentation
      Minamiminowa
    • Year and Date
      2012-01-31
    • Related Report
      2012 Final Research Report
  • [Presentation] Perspectives for Animal Biotechnology2012

    • Author(s)
      Hiroshi Kagami
    • Organizer
      International Symposium on Animal Biotechnology
    • Place of Presentation
      長野県、信州大学(招待講演)
    • Year and Date
      2012-01-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] 幹細胞分化制御による鳥類生殖工学への応用2011

    • Author(s)
      鏡味裕、中村隼明、宮原大地、森貴史、渡辺晴陽、大西翔、砂岡耕平、田中健生、田村芽衣、小野珠乙、松原悠子、韮澤圭二郎、田上貴寛.
    • Organizer
      104回日本繁殖生物学会大会
    • Place of Presentation
      盛岡
    • Year and Date
      2011-09-02
    • Related Report
      2012 Final Research Report
  • [Presentation] ニワトリ幹細胞の培養条件検討と単一幹細胞培養への活用.2011

    • Author(s)
      鏡味裕、中村隼明、宮原大地、森貴史、渡辺晴陽、今井一樹、中村幸夫、小野珠乙、松原悠子、中村俊、小柴満美子、韮澤圭二郎、田上貴寛.
    • Organizer
      日本畜産学会114回大会
    • Place of Presentation
      十和田
    • Year and Date
      2011-08-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 迅速簡便法による各ニワトリ品種の始原生殖細胞の採取と特性評価2011

    • Author(s)
      鏡味裕、臼井文武、中村隼明、山本耕裕、宮原大地、森貴史、渡辺晴陽、今井一樹、小野珠乙、松原悠子、韮澤圭二郎、田上貴寛.
    • Organizer
      日本家禽学会 2011 年度春季大会
    • Place of Presentation
      厚木
    • Year and Date
      2011-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] Effect ofX-ray Irradiation of Primordoal Germ Cell-depletion in Chicken Embryos.2011

    • Author(s)
      Nakamura, Y., Usui, F., Miyahara, D., Mori, T., Watanabe, H., Ono, T., Takeda, K., Nirasawa, Kagami, H., Tagami, T
    • Organizer
      th Asia Pacific Poultry Conference
    • Place of Presentation
      Taipei
    • Year and Date
      2011-03-22
    • Related Report
      2012 Final Research Report
  • [Presentation] Mixed Sex Chimeras and Sex Reversal2011

    • Author(s)
      Hiroshi Kagami
    • Organizer
      First International Symposium on Propagation of Endangered Species of Birds
    • Place of Presentation
      エミレーツパレスホテル(アブダビ市)
    • Year and Date
      2011-02-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Mixed Sex Chimeras and Sex Reversal.2011

    • Author(s)
      Kagami, H.
    • Organizer
      1stInternational Symposium on Conservation & Propagation of Endangered Species of Birds.
    • Place of Presentation
      Abu Dhabi
    • Year and Date
      2011-02-08
    • Related Report
      2012 Final Research Report
  • [Presentation] Avian Stem Cells and the Application for Biotechnology.2010

    • Author(s)
      Kagami, H., Usui, F., Nakamura, Y., Kashiwagi, M., Yamamoto, Y., Ono, T., Tagami, T.,Nirasawa, K., Matsubara, Y., Petitte, J.N.
    • Organizer
      43rd Annual Meeting for the Japanese Society of Developmental Biologist
    • Place of Presentation
      Kyoto
    • Year and Date
      2010-06-21
    • Related Report
      2012 Final Research Report
  • [Presentation] ニワトリ始原生殖細胞の利用:移動様式の解明と凍結保存・移植・除去技術の確立.2010

    • Author(s)
      中村隼明、宮原大地、森貴史、今井一樹、渡辺晴陽、小野珠乙、武田久美子、韮澤圭二郎、鏡味裕、田上貴寛.
    • Organizer
      日本分子生物学会第10回春季シンポジウム
    • Place of Presentation
      松島
    • Year and Date
      2010-06-07
    • Related Report
      2012 Final Research Report
  • [Presentation] ニワトリ幹細胞・始原生殖細胞の遺伝的特徴解析と分化制御の試み2010

    • Author(s)
      宮原大地、今井一樹、森貴史、坂田〓子、坪井友子、都築明香里、中村隼明、臼井文武、山本耕裕、田上貴寛、韮澤圭二郎、松原悠子、川口耕一郎、藤井博、小野珠乙、鏡味裕
    • Organizer
      日本分子生物学会第10回春季シンポジウム
    • Place of Presentation
      松島
    • Year and Date
      2010-06-07
    • Related Report
      2012 Final Research Report
  • [Presentation] ニワトリ幹細胞・始原生殖細胞の培養と発生・分化における特徴把握2010

    • Author(s)
      鏡味裕、臼井文武、中村隼明、柏木まや、伊東陽平、山本耕裕、田上貴寛、韮澤圭二郎、松原悠子、小野珠乙
    • Organizer
      日本畜産学会 112回大会
    • Place of Presentation
      東京
    • Year and Date
      2010-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 始原生殖細胞の新規採取法の開発と生殖細胞キメラ作出への活用.発生・分化における特徴把握.2010

    • Author(s)
      鏡味裕、柏木まや、大屋智子、臼井文武、中村隼明、山本耕裕、小野珠乙
    • Organizer
      日本畜産学会 112回大会
    • Place of Presentation
      始原生殖細胞の新規採取法の開発と生殖細胞キメラ作出への活用.発生・分化における特徴把握.
    • Year and Date
      2010-03-29
    • Related Report
      2012 Final Research Report
  • [Presentation] 幹細胞・生殖細胞の遺伝的特徴把握と鳥類における発生・再生制御への活用の試み.2009

    • Author(s)
      鏡味裕、臼井文武、中村隼明、山本耕裕、大友朝子、柏木まや、田上貴寛、韮澤圭二郎、松原悠子、小野珠乙
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-10
    • Related Report
      2012 Final Research Report
  • [Presentation] 始原生殖細胞とその収集時に温存したニワトリ胚からの個体再生2009

    • Author(s)
      中村隼明、臼井文武、柏木まや、坂田詢子、小野珠乙、武田久美子、韮澤圭二郎、鏡味裕、田上貴寛
    • Organizer
      日本畜産学会 111回大会
    • Place of Presentation
      那覇
    • Year and Date
      2009-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 胚盤葉明域中央部への局所的 UV 照射及び生殖新月環への UV 照射を用いたレシピエント胚由来PGCs 数減少の試み2009

    • Author(s)
      柏木まや、中村隼明、山本耕裕、臼井文武、川越雄太、松原和衛、小野珠乙、鏡味裕
    • Organizer
      日本畜産学会111回大会
    • Place of Presentation
      那覇
    • Year and Date
      2009-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] ニワトリ幹細胞及び始原生殖細胞の発生に伴う遺伝子発現の解析と分化制御への活用の試み2009

    • Author(s)
      鏡味裕、臼井文武、中村隼明、山本耕裕、大友朝子、柏木まや、片倉真沙美、田上貴寛、韮澤圭二郎、松原悠子、小野珠乙
    • Organizer
      日本畜産学会111回大会
    • Place of Presentation
      那覇
    • Year and Date
      2009-09-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 凍結融解操作がニワトリ始原生殖細胞の生存性および生殖巣移住能へ及ぼす影響2009

    • Author(s)
      中村隼明、臼井文武、柏木まや、坂田詢子、小野珠乙、武田久美子、韮澤圭二郎、鏡味裕、田上貴寛
    • Organizer
      日本家禽学会 2009 年度秋季大会
    • Place of Presentation
      那覇
    • Year and Date
      2009-09-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 造血幹細胞同定を目的とした致死量放射線量の探索.2009

    • Author(s)
      臼井文武、中村隼明、小野珠乙、鏡味裕.
    • Organizer
      日本家禽学会 2009 年度秋季大会
    • Place of Presentation
      那覇
    • Year and Date
      2009-09-26
    • Related Report
      2012 Final Research Report
  • [Presentation] 宿主の内因性始原生殖細胞の除去によるドナー由来の生殖系譜に置換されたキメラニワトリの作出用.2009

    • Author(s)
      中村隼明、臼井文武、柏木まや、坂田詢子、小野珠乙、武田久美子、韮澤圭二郎、鏡味裕、田上貴寛
    • Organizer
      第102回日本繁殖生物学会大会
    • Place of Presentation
      奈良
    • Year and Date
      2009-09-10
    • Related Report
      2012 Final Research Report
  • [Presentation] Avian stem cells & PGCs for developmental manipulation

    • Author(s)
      Hiroshi Kagami
    • Organizer
      7th International Chick Meeting
    • Place of Presentation
      Nagoya
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] Future possibility and current status of animal stem cell technology

    • Author(s)
      Hiroshi Kagami
    • Organizer
      SRD-NILGS The 2nd Japan-korea-China Joint Symposium
    • Place of Presentation
      Tsukuba
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Book] 鳥類の受精、発生、胚操作. 新動物生殖学(佐藤英明編著)2011

    • Author(s)
      鏡味裕
    • Publisher
      朝倉書店
    • Related Report
      2012 Final Research Report
  • [Book] ニワトリ初期胚における DNA メチル化状態の変化. DNA 多型 Vol. 17(日本 DNA 多型学会編)2009

    • Author(s)
      臼井文武、小野珠乙、鏡味裕
    • Publisher
      東洋書店
    • Related Report
      2012 Final Research Report
  • [Book] DNA多型Vol.17(分担)2009

    • Author(s)
      鏡味裕
    • Total Pages
      296
    • Publisher
      東洋書店
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://karamatsu.shinshu-u.ac.jp/lab/ono_kagami/hasseiken.htm

    • Related Report
      2012 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2019-07-29  

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