Studies on the mechanism of antimicrobial peptide synthesis for a strategy to enhance the innate immunity in hen oviduct

• Project/Area Number: 21380174
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied animal science
• Research Institution: Hiroshima University
• Principal Investigator: YOSHIMURA Yukinori 広島大学, 大学院・生物圏科学研究科, 教授 (10167017)
• Co-Investigator(Renkei-kenkyūsha): ISOBE Naoki 広島大学, 大学院・生物圏科学研究科, 准教授 (80284230)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000); Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2010: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 鳥類 / 卵管 / 自然免疫 / 抗菌ペプチド / 粘膜バリア / 感染防御 / サイトカイン / 繁殖 / 粘液糖類 / 粘液糖鎖

Research Abstract

The aim of this study was to determine the host defense functions by innate immune system in the hen oviduct. The results showed the first defense systemwas formed by mucosal barrier with mucin layer and epithelial cell tight junctions, induction of immunoresponse by cytokines, and synthesis of avian β-defensins (AvBDs). Decline of mucosal barrier system in molting birds was likely to be one of the reasons whyinfectious risk increases in them. The current study suggested a new mechanism by which AvBDs are induced, namely IL1β upregulates the expression of some AvBDs.

Research Products

• [Journal Article] Induction of avian β-defensins by CpG oligodeoxynucleo- tides and proinflammatory cytokines in hen vaginal cells in vitro (2013)
  • Author(s): Sonoda Y, Abdel Mageed AM, Isobe N, Yoshimura Y
  • Journal Title: Reproduction Volume: 145(6) Pages: 621-631
  • Peer Reviewed
• [Journal Article] Expression of tight junction molecule "claudins" in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens. (2013)
  • Author(s): Ariyadi B, Isobe N, Yoshimura Y
  • Journal Title: Theriogenology Volume: 79(2) Pages: 211-218
  • Peer Reviewed
• [Journal Article] Effects of Repeated Lipopolysaccharide Stimulation on the Development of Antigen-presenting Cells and T Cells Pool in Hen Vagina (2013)
  • Author(s): Nii T, Isobe N, Yoshimura Y
  • Journal Title: The Journal of Poultry Science Volume: 50 Issue: 1 Pages: 83-89
  • DOI: 10.2141/jpsa.0120086
  • NAID: 130004446389
  • ISSN: 1346-7395, 1349-0486
  • Peer Reviewed
• [Journal Article] Induction of avian b-defensins by CpG oligodeoxynucleotides and proinflammatory cytokines in hen vaginal cells in vitro (2013)
  • Author(s): Yuka Sonoda, Ahmad M Abdel Mageed, Naoki Isobe and Yukinori Yoshimura
  • Journal Title: Reproduction Volume: 145 Issue: 6 Pages: 621-631
  • DOI: 10.1530/rep-12-0518
  • Peer Reviewed
• [Journal Article] Effects of repeated lipopolysaccharide stimulation on the development of antigenpresenting cells and T cells pool in hen vagina. (2012)
  • Author(s): Nii T, Isobe N, Yoshimura Y
  • Journal Title: J Poult Sci Volume: 50 Pages: 83-89
  • Peer Reviewed
• [Journal Article] Differences in the mucosal surface barrier formed by mucin in the lower oviductal segments between laying and molting hens (2012)
  • Author(s): Ariyadi B, Isobe N, Yoshimura Y
  • Journal Title: Poult Sci Volume: 91(5) Pages: 1173-1178
  • Peer Reviewed
• [Journal Article] Differences in the mucosal surface barrier formed by mucin in the lower oviductal segments between laying and molting hens (2012)
  • Author(s): Ariyadi B, Isobe N, Yoshimura Y
  • Journal Title: Poultry Science Volume: (In press)
  • Peer Reviewed
• [Journal Article] Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and the subsequent recruitment of immunocompetent cells in the oviduct of laying and molting hens (2011)
  • Author(s): Nii T, Sonoda Y, Isobe N, Yoshimura Y
  • Journal Title: Poult Sci Volume: 90(10) Pages: 233-2341
  • Peer Reviewed
• [Journal Article] Changes in the density of immunoreactive avian beta-defensin-3 and -11 in the hen uterus in response to lipopolysaccharide inoculation. (2011)
  • Author(s): Abdel Mageed AM, Isobe N and Yoshimura Y
  • Journal Title: J Poult Sc Volume: 48(2) Pages: 73-77
  • Peer Reviewed
• [Journal Article] Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and the subsequent recruitment of immunocompetent cells in the oviduct of laying and molting hens (2011)
  • Author(s): Nii T, Sonoda Y, Isobe N, Yoshimura Y
  • Journal Title: Poultry Science Volume: 90 Issue: 10 Pages: 2332-2341
  • DOI: 10.3382/ps.2011-01596
  • Peer Reviewed
• [Journal Article] Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and influx of leukocytes in hen ovary (2011)
  • Author(s): Abdelsalam M, Isobe N, Yoshimura Y
  • Journal Title: Poultry Science Volume: 90 Issue: 9 Pages: 2054-2062
  • DOI: 10.3382/ps.2011-01394
  • Peer Reviewed
• [Journal Article] Changes in the density of immunoreactive avian beta-defensin-3 and -11 in the hen uterus in response to lipopolysaccharide inoculation. (2011)
  • Author(s): Abdel Mageed AM, Isobe N, Yoshimura Y
  • Journal Title: Journal of Poultry Science Volume: 48 Pages: 73-77
  • Peer Reviewed
• [Journal Article] Changes in the localization of immunoreactive avian beta-defensin-8-10 and -12 in hen ovarian follicles during follicular growth. (2010)
  • Author(s): Abdelsalam M, Isobe N, Yoshimura Y
  • Journal Title: Journal of Poultry Science Volume: 47 Pages: 77-84
  • NAID: 10026253225
  • Peer Reviewed
• [Journal Article] Immunolocalization of avian beta-defensins in the hen oviduct and their changes in the uterus during eggshell formation (2009)
  • Author(s): Abdel Mageed AM, Isobe N and Yoshimura Y
  • Journal Title: Reproduction Volume: 138(6) Pages: 971-978
  • Peer Reviewed
• [Presentation] Expressions of tight junction molecule "claudins" in the lower oviductal segments and their changes with gonadal steroids stimulation in the laying and molting hens (2012)
  • Author(s): Bambang Ariyadi, Naoki Isobe, Yukinori Yoshimura
  • Organizer: 日本家禽学会2012年度春季大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-03-30
• [Presentation] ニワトリ卵管のCpG-オリゴDNAによる炎症性サイトカインと抗菌ペプチドの誘導性 (2012)
  • Author(s): 園田由佳・磯部直樹・吉村幸則
  • Organizer: 日本家禽学会2012年度春季大会
  • Place of Presentation: 名古屋市
  • Year and Date: 2012-03-30
• [Presentation] Regulation of β-defensins expression by microbial components and proinflammatory cytokines in hen oviduct (2012)
  • Author(s): Yoshimura Y., Abdel-Mageed A., Sonoda Y., Nii T., Ariyadi B., and Isobe N
  • Organizer: 10th Int Symposium on Avian Endocrinol
  • Place of Presentation: 長良川コンベンションセンター, 岐阜市)
• [Presentation] Effects of estradiol on the mucin mRNA expression in the lower oviductal segments of hens (2012)
  • Author(s): Bambang Ariyadi, Naoki Isobe, Yukinori Yoshimura
  • Organizer: 日本家禽学会2012年度秋季大会
  • Place of Presentation: 高松市
• [Presentation] Regulation of β-defensins expression by microbial components and proinflammatory cytokines in hen oviduct. (2012)
  • Author(s): Yukinori Yoshimura, Ahmad Abdel-Mageed, Yuka Sonoda, Takahiro Nii, Bambang Ariyadi, and Naoki Isobe
  • Organizer: 10th International Symposium on Avian Endocrinology
  • Place of Presentation: 岐阜市
• [Presentation] Effects of repeated stimulation by lipopolysaccharide on the antigen presenting cell-T cell associated immunity in hen vagina. (2012)
  • Author(s): Takahiro Nii, Naoki Isobe, and Yukinori Yoshimura
  • Organizer: 10th International Symposium on Avian Endocrinology
  • Place of Presentation: 岐阜市
• [Presentation] ニワトリ腔部のMHCクラスIIおよびT細胞系免疫能に及ぼすLPS反復刺激の影響 (2012)
  • Author(s): 新居隆浩・磯部直樹・吉村幸則
  • Organizer: 日本畜産学会第115回大会
  • Place of Presentation: 名古屋市
• [Presentation] Innate Immune system in hen oviduct (2011)
  • Author(s): Yoshimura Y.
  • Organizer: Korea-Japan Joint Symposium on Avian Biology
  • Place of Presentation: ソウル、韓国
  • Year and Date: 2011-07-11
• [Presentation] Inductions in the recruitment of T cells and expression of chemokines in response to pathogenic agents in the lower oviducts between laying and molting hens.Korea-Japan Joint Symposium on Avian Biology 2011(2011.7.11,ソウル大、韓国) (2011)
  • Author(s): Nii T, Sonoda Y, Isobe N, Yoshimura Y
  • Organizer: Korea-Japan Joint Symposium on Avian Biology
  • Place of Presentation: ソウル、韓国
  • Year and Date: 2011-07-11
• [Presentation] 産卵鶏の卵管膣部におけるリポ多糖刺激に伴う炎症性サイトカインおよび抗菌ペプチドの発現変化 (2011)
  • Author(s): 園田由佳・磯部直樹・吉村幸則
  • Organizer: 2011年度関西畜産学会
  • Place of Presentation: 岡山市
• [Presentation] Differences in the localization and molecular size of glycoproteins forming the mucosal barrier in the lower part of oviduct in laying and molting hens (2011)
  • Author(s): Bambang Ariyadi, Naoki Isobe, Yukinori Yoshimura
  • Organizer: 2011年度関西畜産学会
  • Place of Presentation: 岡山市
• [Presentation] Host Defense Systems in the Oviduct and Their Changes with Egg-laying Activity and Aging in Hens.Proceedings of 14th, vol.1, 351-354, 2010((plenary session), Pintung, Taiwan) (2010)
  • Author(s): Yoshimura Y
  • Organizer: アジア大洋州畜産学会議
  • Place of Presentation: 台湾(招待講演)
• [Remarks] 広島大学大学院生物圏科学研究科家畜生体機構学研究室
  • URL: http://home.hiroshima-u.ac.jp/anat/