Analysis of lethal pathogenicity mechanism of herpesvirus in non-natural host

• Project/Area Number: 21380179
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Gifu University
• Principal Investigator: FUKUSHI Hideto 岐阜大学, 応用生物科学部, 教授 (10156763)
• Co-Investigator(Kenkyū-buntansha): OHYA Kenji 岐阜大学, 応用生物科学部, 准教授 (50402219)
• Co-Investigator(Renkei-kenkyūsha): MATSUMURA Tomio 日本中央競馬会競走馬総合研究所, 栃木支所, 室長 (20446511) ; TSUJIMURA Koji 日本中央競馬会競走馬総合研究所, 栃木支所, 室員 (50446514)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000); Fiscal Year 2011: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2010: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2009: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 病原微生物 / ヘルペスウイルス / 神経病原性

Research Abstract

The object of this research is to reveal the molecular mechanism of herpesvirus lethal encephalitis in non-natural host. Equine herpesviruses(EHVs) 1 and 9 were used. Virus genes involving in the lethal encephalitis in non-natural host were identified. Comparison of neuropathogenic and non-neuropathogenic EHV-1s showed nine gene differences between their whole genome sequences. Comprehensive transcriptome analysis in EHV-infected cells were examined. Several viral and host genes were identified as variable transcript encoded genes in EHV-infected cells.

Research Products

• [Journal Article] Functional characterization of EUL47 in productive replication morphogenesis and infectivity of equine herpesvirus 1 (2011)
  • Author(s): Mi Htay Htay Yu, Samy Gomaa Ahmed Kasem, Norio Yoshizaki, Ochir Pagamjav, Tsuyoshi Yamaguchi, Kenji Ohya, Hideto Fukushi
  • Journal Title: Virus Research Volume: vol.163 Pages: 310-319
  • Peer Reviewed
• [Journal Article] Functional characterization of EUL47 in productive replication, morphogenesis and infectivity of equine herpesvirus 1 (2011)
  • Author(s): Yu MH, Kasem S, Yoshizaki N, Pagamjav O, Yamaguchi T, Ohya K, Fukushi H
  • Journal Title: Virus Research Volume: 163 Issue: 1 Pages: 310-319
  • DOI: 10.1016/j.virusres.2011.10.021
  • Peer Reviewed
• [Journal Article] Kinetics and pathogenicity of oral infection by equine herpesvirus-9 in mice and suckling hamsters (2011)
  • Author(s): El-Nahass E, El-Habashi N, Abdelaziz AA, Navel M, Kasem S, Fukushi H, Tuji H, Hirata A, Sakai H, Yanai T
  • Journal Title: J.Comp.Pathol. Volume: 146 Issue: 2-3 Pages: 211-222
  • DOI: 10.1016/j.jcpa.2011.01.009
  • Peer Reviewed
• [Journal Article] Effects of equine herpesvirus-9 infection in pregnant mice and hamsters (2011)
  • Author(s): El-Habashi N, El-Nahass E, Fukushi H, Nayel M, Hibi D, Sakai H, Yanai T
  • Journal Title: J.Comp.Pathol Volume: 144 Pages: 103-112
  • Peer Reviewed
• [Journal Article] The ORF37(UL24) is an neuropathogenicity determinant of equine herpesvirus 1(EHV-1) in the mouse encephalitis model (2010)
  • Author(s): Samy Kasem, Mi Htay Htay Yu, Souichi Yamada, Akari Kodaira, Tomio Matsumura, Koji Tsujimura, Hanafy Madbouly, Tsuyoshi Yamaguchi, Kenji Ohya, Hideto Fukushi
  • Journal Title: Virology Volume: vol.400 Pages: 259-270
  • Peer Reviewed
• [Journal Article] Diverse pathogenicity of equine herpesvirus 1(EHV-1) isolates in CBA mouse mode (2010)
  • Author(s): Mi Htay Htay Yu, Samy Gomaa Ahmed Kasem, Koji Tsujimura, Tomio Matsumura, Tokuma Yanai, Tsuyoshi Yamaguchi, Kenji Ohya, Hideto Fukushi
  • Journal Title: Journal of Veterinary Medical Sciences Volume: vol.72 Pages: 259-270
  • Peer Reviewed
• [Journal Article] Diverse pathogenicity of equine herpesvirus 1 (EHV-1) isolates in CBA mouse model. (2010)
  • Author(s): Yu MH, Kasem SG, Tsujimura K, Matsumura T, Yanai T, Yamaguchi T, Ohya K, Fukushi H.
  • Journal Title: J.Vet.Med.Sci. Volume: 72 Pages: 301-306
  • NAID: 130000134545
  • Peer Reviewed
• [Journal Article] The ORF37 (UL24) is a neuropathogenicity determinant of equine herpesvirus 1 (EHV-1) in the mouse encephalitis model (2010)
  • Author(s): Kasem S, Yu MH, Yamada S, Kodaira A, Matsumura T, Tsujimura K, Madbouly H, Yamaguchi T, Ohya K, Fukushi H.
  • Journal Title: Virology Volume: 400 Pages: 259-270
  • Peer Reviewed
• [Journal Article] Experimental infranasal infection of equine herpesvirus 9 (EHV-9) in suckling hamsters : kinetics of viral transmission and inflammation in the nasal cavity and brain. (2010)
  • Author(s): El-Habashi N, El-Nahass el-S, Fukushi H, Hibi D, Sakai H, Sasseville V, Yanai T.
  • Journal Title: J.Neurovirol. Volume: 16 Pages: 242-248
  • Peer Reviewed
• [Journal Article] Cynomolgus monkeys (Macaca fascicularis) may not become infected with equine herpesvirus 9 (2010)
  • Author(s): Kodama A, Yanai T, Kubo M, El-Habashi N, Kasem S, Sakai H, Masegi T, Fukushi, H, Kuraishi T, Yoneda M, Hattori S, Kai C.
  • Journal Title: J.Med.Primatol. Volume: 40 Pages: 18-20
  • Peer Reviewed
• [Journal Article] The ORF37 (UL24) is a neuropathogenicity determinant of equine herpesvirus 1 (EHV-1) in the mouse encephalitis model (2010)
  • Author(s): Kasem, et al.
  • Journal Title: Virology 400 Pages: 259-270
  • Peer Reviewed
• [Journal Article] Diverse Pathogenicity of Equine Herpesvirus 1 (EHV-1) Isolates in CBA Mouse Model (2009)
  • Author(s): Yu, et al.
  • Journal Title: J.Vet.Med.Sci. 72 Pages: 301-306
  • Peer Reviewed
• [Presentation] The effect of point mutation found in Equine Herpesvirus BAC clone, Ab4p attB, to the pathogenicity in the hamster model (2011)
  • Author(s): 郭小芹,岡田彩加, Abdelaziz Amira,大屋賢司,福士秀人
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-09-20
• [Presentation] ウマヘルペスウイルス1型テグメントタンパクVP22の細胞内動態解析 (2011)
  • Author(s): 岡田彩加,羽入さち子,大屋賢司,福士秀人
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-09-20
• [Presentation] ウマヘルヘ・スウイルス1型ORF75のRK13細胞における細胞内局在 (2011)
  • Author(s): 井爪聡子,辻村行司,松村富夫,大屋賢司,福士秀人
  • Place of Presentation: 大阪府立大学
  • Year and Date: 2011-09-20
• [Presentation] ウマヘルペスウイルス1型ORF75のRK13細胞における細胞内局在 (2011)
  • Author(s): 井爪聡子, 辻村行司, 松村富夫, 大屋賢司, 福士秀人
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
• [Presentation] Kinetics and Pathogenicity of Oral Infection by Equine Herpesvirus-9 in Mice and Suckling Hamsters (2011)
  • Author(s): El-NahassEl-Shaymaa, El-DakhlyKhaled, AsaSamah Abou, 福士秀人, 平田暁大, 酒井洋樹, 柳井徳磨
  • Organizer: 第152回日本獣医学会学術集会
  • Place of Presentation: 大阪府立大学
• [Presentation] Affection of Equine Herpesvirus 9 (EHV-9) in pregnant rats (2010)
  • Author(s): El-HabashiNagwan, El-Nahass El Shymaa, Mohamed Nayel,福士秀人, 酒井洋樹, 柳井徳磨
  • Organizer: 日本獣医学会学術集会第150回
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-17
• [Presentation] In vivo and in virto Characterization of Equine herpesvirus-1 thymidine kinase-deficient mutants (2010)
  • Author(s): KasemSamy, YuMi Htay Htay,大屋賢司,福士秀人
  • Organizer: 日本獣医学会学術集会第150回
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-16
• [Presentation] 蛍光蛋白質融合テグメントVP22保有ウマヘルペスウイルス1型感染細胞におけるVP22動態のリアルタイム解析 (2010)
  • Author(s): 羽入さち子,小平彩里,山田壮一, Samy, Kasem, Mi Htay Htay Yu,大屋賢司,福士秀人
  • Organizer: 日本獣医学会学術集会第150回
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-16
• [Presentation] ウマヘルペスウイルス増殖に優れたウマ胎仔脳由来細胞株の樹立 (2010)
  • Author(s): 岡田彩加, Samy Kasem,辻村行司,松村富夫,大屋賢司,福士秀人
  • Organizer: 日本獣医学会学術集会第150回
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-16
• [Presentation] Functional characterization of ORF 13 of EHV-1 (2010)
  • Author(s): YuMi Htay Htay, 吉崎範夫, KasemSamy, 大屋賢司, 福士秀人
  • Organizer: 日本獣医学会学術集会第150回
  • Place of Presentation: 帯広畜産大学
  • Year and Date: 2010-09-16
• [Book] Virology and Pathology of Encephalitis in Alien Hosts by Neurotropic Equine Herpesvirus 9. In "Non-Flavivirus Encephalitis", ed. Sergey Tkachev (2011)
  • Author(s): Hideto Fukushi and Tokuma Yanai
  • Publisher: In Tech, Croatia
• [Book] Equine Herpesvirus 9(EHV-9)-induced encephalitis in non-human primates. In "Non-flavivirus Encephalitis" ed. Sergey Tkachev (2011)
  • Author(s): Tokuma Yanai, Atsushi Kodama, Hiroki Sakai, Hideto Fukushi, Takeshi Kuraishi, Seisaku Hattori, Chieko Cai
  • Publisher: In Tech, Croatia
• [Book] Virology and Pathology of Encephalitis in Alien Hosts by Neurotropic Equine Herpesvirus 9. In "Non-Flavivirus Encephalitis", ed.Sergey Tkachev, pp 127-146 (2011)
  • Author(s): Fukushi, H., Yanai, T.
  • Total Pages: 360
  • Publisher: In Tech, Croatia
• [Book] Equine Herpesvirus 9(EHV-9)-induced encephalitis in non-human primates.In "Non-flavivirus Encephalitis" ed.Sergey Tkachev, pp.147-156 (2011)
  • Author(s): Yanai, T., Kodama, A., Sakai, H., Fukushi, H., Hattori, S., Kai, C.
  • Total Pages: 360
  • Publisher: In Tech, Croatia