Mechanism of nano-particle translocation at the air-bloodbarrier and its effects on health

• Project/Area Number: 21380181
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Basic veterinary science/Basic zootechnical science
• Research Institution: Tottori University
• Principal Investigator: SHIMADA Akinori 鳥取大学, 農学部, 教授 (20216055)
• Co-Investigator(Renkei-kenkyūsha): TAKANO Hirohisa 独立行政法人国立環境研究所, 環境健康領域, 領域長 (60281698)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: ナノ粒子 / 空気血液関門 / 突破機序 / エンドサイトーシス / カベオラ / 免疫組織化学 / 免疫電顕 / 浮遊粒子状物質 / フラーレン

Research Abstract

After exposure of nano-particles(C60 Fullerene, colloidal gold) into the respiratory tract of mice, localization and translocation pathway of the particles, effects of the particles on the tissues were examined with the use of light microscopy, immunohistochemistry and electron microscopy. Nano-particles showed no signs of tissue damage in the respiratory system. Association of diffusion and caveolae mediated endocytosis were indicated as the translocation pathway of the exposed nano-particles.

Research Products

• [Journal Article] Caveolae-mediated endocytosis of intratracheally instilled gold colloid nanoparticles at the air-blood barrier in mice (2012)
  • Author(s): Naota M, Shimada A, Morita T, Yamamoto Y, Inoue K, Takano H.
  • Journal Title: Toxicologic Pathology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Translocation pathway of the intratracheally instilled C60 Fullerene from the lung into the blood circulation in mouse : possible association of diffusion and caveolae mediated pinocytosis (2009)
  • Author(s): Naota M, Shimada A, Morita T, Inoue K, Takano H.
  • Journal Title: Toxicologic Pathology Volume: Vol.37 Pages: 456-462
  • Peer Reviewed
• [Journal Article] Translocation pathway of the intratracheally instilled C60 Fullerene from the lung into the blood circulation in mouse : possible association of diffusion and caveolae mediated pinocytosis. (2009)
  • Author(s): Naota M, Shimada A, Morita T, Inoue K, Takano H.
  • Journal Title: Toxicol Pathol 37巻 Pages: 456-462
  • Peer Reviewed
• [Presentation] Pathological study on the pulmonary toxicity induced by the intratracheally instilled Asian sand dust in mice (2009)
  • Author(s): Naota M.
  • Organizer: 4th Asian Society of Veterinary Pathologists Conference
  • Place of Presentation: Chulalongkorn University, Bangkok, Thailand
  • Year and Date: 2009-11-19
• [Presentation] 気管内投与黄砂粒子の急性肺毒性についての病理学的研究 (2009)
  • Author(s): 直田みさき
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取市
  • Year and Date: 2009-09-26