Structural analysis of zoonosis-related high-molecular protein aggregation by double electron-electron resonance (DEER) technique

• Project/Area Number: 21380185
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Hokkaido University
• Principal Investigator: INANAMI Osamu 北海道大学, 大学院・獣医学研究科, 教授 (10193559)
• Co-Investigator(Kenkyū-buntansha): INABA Mustumi 北海道大学, 大学院・獣医学研究科, 教授 (00183179) ; HORIUCHI Motohiro 北海道大学, 大学院・獣医学研究科, 教授 (30219216) ; KUWABARA Mikinori 北海道大学, 大学院・獣医学研究科, 名誉教授 (10002081) ; YAMAMORI Tohru 北海道大学, 大学院・獣医学研究科, 准教授 (00512675)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2011: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2009: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: プリオン / 構造生物学 / ESR分光法 / ニトロオキシドプローブ / 距離情報 / 部位特異的スピンラベル法 / 人獣共通感染症 / インフルエンサ / インフルエンザ

Research Abstract

In this study, to obtain the structural information for this pathological fibrous form of PrPs', we measured the intermolecular distance in the pathological fibrous form with D178N mutation by the site-directed spin labeling (SDSL) with continuous wave (CW) ESR (X-band) and pulsed ESR (Q-band). After the formation of the fibrous structure by exposure to 1M of guanidine hydrochloride in pH4.0, the ESR spectra were measured by CW-ESR. The interspin-distances in pathological fibrous form were measured by continuous wave ESR (CW-ESR) and the four-pulse double electron-electron resonance (DEER) technique. The information of intermolecular distances will be useful to estimate the pathogenic fibrous structure of the familial prion disease, since it is difficult to analyze the macromolecules such as the fibrous protein by NMR or X-ray crystallography.

Research Products

• [Journal Article] A nucleoside anticancer drug, 1-(3-C-ethynyl-Q-D-ribo-pentofuranosyl ) cytosine (TAS106), sensitizes cells to radiation by suppressing BRCA2 expression (2011)
  • Author(s): Meike S., Yamamori T., Yasui H., Eitaki M., Matsuda A., Morimatsu M., Fukushima M., Yamasaki Y., and Inanami O.
  • Journal Title: Molecular Cancer Volume: 10 Issue: 1 Pages: 92-92
  • DOI: 10.1186/1476-4598-10-92
  • Peer Reviewed
• [Journal Article] 8-Aminoadenosine Enhances Radiation-induced Cell Death in Human Lung Carcinoma A549 Cells (2011)
  • Author(s): Meike S., Yamamori T., Yasui H., Eitaki M., Matsuda A., and Inanami O
  • Journal Title: Journal of Radiation Research Volume: 52 Issue: 4 Pages: 456-463
  • DOI: 10.1269/jrr.10188
  • NAID: 10029122169
  • ISSN: 0449-3060, 1349-9157
  • Peer Reviewed
• [Journal Article] Oral administration of bovine lactoferrin upregulates neutrophil functions in a dog with familial 2-integrin-related neutrophil dysfunction. (2011)
  • Author(s): Kobayashi S, Abe Y, Inanami 0, Oda S, Yamauchi K, Hankanga C, Yasuda J, Sato R.
  • Journal Title: Veterinary Immunology and Immunopathology Volume: 143 Issue: 1-2 Pages: 155-161
  • DOI: 10.1016/j.vetimm.2011.05.027
  • Peer Reviewed
• [Journal Article] Phosphorylation of p66shc mediates 6-hydroxydopamine cytotoxicity (2011)
  • Author(s): Yamamori, T., Mizobata, A., Saito, Y., Urano, Y., Inanami, O., Irani, K. and Noguchi, N.
  • Journal Title: Free Radical Research Volume: 45 Issue: 3 Pages: 342-350
  • DOI: 10.3109/10715762.2010.532496
  • Peer Reviewed
• [Journal Article] 24(S)-hydroxycholesterol induces neuronal cell death through necroptosis, a form of programmed necrosis. (2011)
  • Author(s): Yamanaka, K., Saito, Y., Yamamori, T., Urano, Y., Noguchi, N.
  • Journal Title: Journal of Biological Chemistry Volume: 286 Issue: 28 Pages: 24666-24673
  • DOI: 10.1074/jbc.m111.236273
  • Peer Reviewed
• [Journal Article] Regulation of DNA repair enzyme AP endonuclease 1 by lifespan regulation-related protein Sirt1. (2011)
  • Author(s): Yamamori, T. and Irani, K.
  • Journal Title: The Journal of the Hokkaido Veterinary Medical Association Volume: 55 Pages: 45-49
• [Journal Article] Regulation of DNA repair enzyme AP endonuclease 1 by lifespanregulation-related protein Sirt1 (2011)
  • Author(s): Yamamori, T. and Irani, K.
  • Journal Title: The Journal of the Hokkaido Veterinary Medical Association Volume: 55 Pages: 45-49
• [Journal Article] 8-Amino-adenosine Enhances Radiation-induced Cell Deathin Human Lung Carcinoma A549 Cells (2011)
  • Author(s): Meike S
  • Journal Title: Journal of Radiation Research Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Phosphorylation of p66shc mediates 6-hydroxydopamine cytotoxicitY. (2011)
  • Author(s): Yamamori T
  • Journal Title: Free Radical Research Volume: 45 Pages: 342-350
  • Peer Reviewed
• [Journal Article] SIRT1 deacetylates APE1 and regulates cellular base excision repair. (2010)
  • Author(s): Yamamori, T., DeRicco, J., Naqvi A, Hoffman TA., Mattagajasingh, I., Kasuno, K., Jung SB, Kim CS., and Irani, K.
  • Journal Title: Nucleic Acids Research Volume: 38 Pages: 832-845
  • Peer Reviewed
• [Journal Article] Super paramagnetic iron oxide MRI shows defective Kupffer cell uptake function in non-alcoholic fatty liver disease. (2010)
  • Author(s): Asanuma, T., Ono, M., kubota, K., Hirose, A., Hayashi, Y., Saibara, T., Inanami, O., Enzan, H., Onishi, S., Kuwabara, M., and Oben, JA.
  • Journal Title: Gut Volume: 59 Pages: 258-266
  • Peer Reviewed
• [Journal Article] Induction of neurite outgrowth by alpha-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells. (2010)
  • Author(s): Yasui, H., Ito, N., Yamamori, T., Nakamura, H., Okano, J., Asanuma, T., Nakajima, T., Kuwabara, M. , and Inanami, O.
  • Journal Title: Free Radical Research Volume: 44 Pages: 645-654
  • Peer Reviewed
• [Journal Article] A novel copper(II) coordination at His186 in full-length murine prion protein. (2010)
  • Author(s): Watanabe, Y., Hiraoka, W., Igarashi, M., Ito, K., Shimoyama, Y., Horiuchi, M., Yamamori, T., Yasui, H., Kuwabara, M., Inagaki, F., and Inanami, O.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 394 Pages: 522-528
  • NAID: 120002148672
  • Peer Reviewed
• [Journal Article] New insight into hypoxia-targeting cancer therapy. (2010)
  • Author(s): Yasui, H., Yamamori. T., Meike, S., Eitaki, M., Iizuka, D., Kuwabara, M., and Inanami, O.
  • Journal Title: Radiation Biology Research Communications Volume: 45 Pages: 45-57
• [Journal Article] Super paramagnetic iron oxide MRI shows defective Kupffer cell uptake function in non-alcoholic fatty liver disease. (2010)
  • Author(s): Asanuma T
  • Journal Title: Gut. Volume: 59 Pages: 258-266
  • Peer Reviewed
• [Journal Article] Induction of neurite outgrowth by alpha-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathwav in PC12 cells. (2010)
  • Author(s): Yasui H
  • Journal Title: Free Radical Research Volume: 44 Pages: 645-654
  • Peer Reviewed
• [Journal Article] A novel copper(II) coordination at His186 in full-length murine prion protein. (2010)
  • Author(s): Watanabe Y
  • Journal Title: Biochemical and Biophysical Research Communications. Volume: 394 Pages: 522-528
  • Peer Reviewed
• [Journal Article] New insight into Hypoxia-targeting cancer therapy. (2010)
  • Author(s): Yasui H
  • Journal Title: Radiation Biology Research communications (in japanese) Volume: 45 Pages: 45-57
  • Peer Reviewed
• [Journal Article] 低酸素環境を標的とした癌治療の新たな展開 (2010)
  • Author(s): 安井博宣、山盛徹、女池俊介、永瀧正人、飯塚大輔、桑原幹典、稲波修
  • Journal Title: Free Radic Res. 45 Pages: 58-70
• [Journal Article] Canine neutrophil dysfunction caused by downregulation of beta2-integrin expression without mutation. (2009)
  • Author(s): Kobayashi S., Sato R., Abe Y., Inanami O., Yasui H., Omoe K., Yasuda J., Hankanga C., Oda S., and Sasaki J.
  • Journal Title: Veterinary Immunology and Immunopathology. Volume: 130 Pages: 187-196
  • Peer Reviewed
• [Journal Article] Redox regulation in radiation-nduced cytochrome c release from mitochondria of human lung carcinoma A549 cells. (2009)
  • Author(s): Ogura A., Oowada S., Kon Y., Hirayama A., Yasui H., Meike S., Kobayashi S., Kuwabara M., and Inanami O.
  • Journal Title: Cancer Letter Volume: 277(1) Pages: 64-71
  • Peer Reviewed
• [Journal Article] Canine neutrophil dysfunction caused by downregulation of beta2-integrin expression without mutation. (2009)
  • Author(s): Kobayashi S, Sato R, Abe Y, Inanami 0, Yasui H, Omoe K, Yasuda J, Hankanga C, Oda S, Sasaki J.
  • Journal Title: Veterinary Immunology and Imunopathology Volume: 130(3-4) Pages: 187-96
  • Peer Reviewed
• [Journal Article] Redox regulation in radiation-induced cytochrome c release from mitochondria of human lung carcinoma A549 cells. (2009)
  • Author(s): Ogura A, Oowada S, Kon Y, Hirayama A, Yasui H, Meike S, Kobayashi S, Kuwabara M, Inanami O.
  • Journal Title: Cancer Lett. 277 Pages: 64-71
  • NAID: 120001253404
  • Peer Reviewed
• [Journal Article] Canine neutrophil dysfunction caused by downregulation of beta2-integrin expression without mutation. (2009)
  • Author(s): Kobayashi S, Sato R, Abe Y, Inanami O, Yasui H, Omoe K, Yasuda J, Hankanga C, Oda S, Sasaki J.
  • Journal Title: Vet Immunol Immunopathol. 130 Pages: 187-196
  • Peer Reviewed
• [Journal Article] Super paramagnetic iron oxide MRI shows defective Kupffer cell uptake function in non-alcoholic fatty liver disease. (2009)
  • Author(s): Asanuma T, Ono M, Kubota K, Hirose A, Hayashi Y, Saibara T, Inanami O, Ogawa Y, Enzan H, Onishi S, Kuwabara M, Oben JA.
  • Journal Title: Gut. 59 Pages: 258-266
  • Peer Reviewed
• [Journal Article] A novel copper(II) coordination at His186 in full-length murine prion protein. (2009)
  • Author(s): Watanabe Y, Hiraoka W, Igarashi M, Ito K, Shimoyama Y, Horiuchi M, Yamamori T, Yasui H, Kuwabara M, Inagaki F, Inanami O.
  • Journal Title: Biochem Biophys Res Commun. 394 Pages: 522-528
  • NAID: 120002148672
  • Peer Reviewed
• [Journal Article] Induction of neurite outgrowth by alpha-phenyl-N-tert-butylnitrone through nitric oxide release and Ras-ERK pathway in PC12 cells.
  • Author(s): Yasui H, Ito N, Yamamori T, Nakamura H, Okano J, Asanuma T, Nakajima T, Kuwabara M, Inanami O.
  • Journal Title: Free Radic Res. (印刷中)
  • Peer Reviewed
• [Presentation] Structual characterization in the mouse prion protein by site-directed spin-labeling and ESR technique (2012)
  • Author(s): 稲波修
  • Organizer: Advanced ESR Studies for New Frontiers in Biofunctional Spin Science and Technology
  • Place of Presentation: 神戸,神戸大瀧川メモリアルホール
  • Year and Date: 2012-11-14
• [Presentation] 放射線誘導性再酸素化の動態と一酸化窒素の関与 (2012)
  • Author(s): 稲波修
  • Organizer: アイソトープ放射線薬学研究会
  • Place of Presentation: KKRホテル札幌(札幌)(招待講演)
  • Year and Date: 2012-03-29
• [Presentation] 放射線誘導性再酸素化の動態と一酸化窒素の関与 (2012)
  • Author(s): 永根大幹、安井博宣、山盛徹、趙松吉、久下祐司、中村秀夫、稲波修
  • Organizer: 第14回癌治療増感研究シンポジウム
  • Place of Presentation: 猿沢荘(奈良)
  • Year and Date: 2012-02-10
• [Presentation] Nitric oxide production associated with radiation-induced tumor reoxygenation (2012)
  • Author(s): Nagane M, Yasui H, Yamamori T, Zhao S, Kuge Y, Nakamura H, Inanami O
  • Organizer: 20^<th> Core-to-Core Seminar International Redox Core Symposium on In Vivo Magnetic Resonance Imaging
  • Place of Presentation: 西鉄グランドホテル(福岡)
  • Year and Date: 2012-02-06
• [Presentation] DISTANCE MEASUREMENTS OF FIBROUS PRION PROTEINS BY SITE-DIRECTED SPIN LABELING(SDSL) AND ESR SPECTROSCOPY (2012)
  • Author(s): Inanami O, Furukawa K, Nakamura T, Yamamori T, Hiraoka W
  • Organizer: 20^<th> Core-to-Core Seminar International Redox Core Symposium on In Vivo Magnetic Resonance Imaging
  • Place of Presentation: 西鉄グランドホテル(福岡)(招待講演)
  • Year and Date: 2012-02-05
• [Presentation] Histological visualization of intermittent hypoxia and its influence on resistance in rat glioma (2012)
  • Author(s): Yasui H, Yamamori T, Matsumoto S, Krishna M, Inanami O
  • Organizer: 19^<th> JSPS Core-to-Core Seminar Winter School in Fukuoka
  • Place of Presentation: 九州大学Innovation Centrer for Medical Redox Navigation(福岡)
  • Year and Date: 2012-02-04
• [Presentation] Distance measurements of fibrous prion proteins by site-directed spin labeling(SDSL) and ESR spectrouscopy (2012)
  • Author(s): 稲波修
  • Organizer: 20^<th> JSPS Coe-to-core Seminar Internatiional Redox Core Symposium on In Vivo Magnetic Resonance Imaging
  • Place of Presentation: 福岡,西鉄グランドホテル
• [Presentation] 脳室内移植グリオーマにおける間欠的低酸素の存在と放射線抵抗性への寄与 (2011)
  • Author(s): 安井博宣、新坊弦也、山盛徹、稲波修
  • Organizer: 第9回がんとハイポキシア研究会
  • Place of Presentation: 学習院大学目白キャンパス(東京)
  • Year and Date: 2011-11-27
• [Presentation] 免疫組織化学と電子スピン共鳴法を用いた腫瘍における放射線照射後の再酸素化機構に関する研究 (2011)
  • Author(s): 永根大幹、安井博宣、山盛徹、中村秀夫、稲波修
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(神戸)
  • Year and Date: 2011-11-19
• [Presentation] 腫瘍組織に特徴的な間欠的低酸素のイメージングと放射線抵抗性への関与 (2011)
  • Author(s): 安井博宣、松元慎吾、新坊弦也、Krishna Murali、山盛徹、Mitchell James、稲波修
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(神戸)
  • Year and Date: 2011-11-19
• [Presentation] 放射線誘導バイスタンター効果が細胞の浸潤能に与える影響 (2011)
  • Author(s): 永瀧正人、山盛徹、安井博宣、稲波修
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(神戸)
  • Year and Date: 2011-11-18
• [Presentation] Methyl-pyruvateによるミトコンドリア機能の修飾と放射線増感効果 (2011)
  • Author(s): 西田直哉、安井博宣、山盛徹、稲波修
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(神戸)
  • Year and Date: 2011-11-17
• [Presentation] 小胞体ストレスがDNA損傷修復機構に及ぼす影響とそれを利用した放射線増感の可能性の検討 (2011)
  • Author(s): 女池俊介、山盛徹、安井博宣、稲波修
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(神戸)
  • Year and Date: 2011-11-17
• [Presentation] 放射線により引き起こされるミトコンドリア由来ROS産生のメカニズム (2011)
  • Author(s): 山盛徹、安井博宣、稲波修
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(神戸)
  • Year and Date: 2011-11-17
• [Presentation] X線照射によるアポトーシスはミトコンドリアが主体となりおこる (2011)
  • Author(s): 馬嶋秀行、犬童寛子、稲波修、幸村知子、中川靖一、松本謙一郎、小澤俊彦
  • Organizer: 日本放射線影響学会第54回大会
  • Place of Presentation: 神戸商工会議所会館(神戸)
  • Year and Date: 2011-11-17
• [Presentation] Structual characterization in the mouse prion protein by site-directed spin-labeling and ESR technique (2011)
  • Author(s): Inanami O, Yamamori T, Yasui H, Kuwabara M, Hiraoka W
  • Organizer: Advanced ESR Studies for New Frontiers in Biofunctional Spin Science and Technology
  • Place of Presentation: 神戸大瀧川メモリアルホール(神戸)(招待講演)
  • Year and Date: 2011-11-13
• [Presentation] p66shcはHSP72との相互作用を介して細胞の放射線感受性を調節する (2011)
  • Author(s): 山盛徹、松下明子、女池俊介、永瀧正人、安井博宣、稲波修
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-09-24
• [Presentation] ビンクリスチンはGEF-H1/RhoA/ROCK/Myosin light chainシグナルを介してMKN45細胞のアメーバ様運動を促進する (2011)
  • Author(s): 永瀧正人、山盛徹、女池俊介、安井博宣、稲波修
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2011-09-22
• [Presentation] 核酸代謝拮抗剤TAS106による相同組換え修復阻害作用を介した放射線増感効果 (2011)
  • Author(s): 女池俊介、山盛徹、安井博宣、松田彰、森松正美、福島正和、山崎靖人、稲波修
  • Organizer: 第152回日本獣医学会学術集会学会
  • Place of Presentation: 大阪府大中百舌鳥キャンパス(大阪)
  • Year and Date: 2011-09-20
• [Presentation] ラット脳腫瘍における間欠的低酸素の可視化と放射線感受性に与える影響 (2011)
  • Author(s): 安井博宣、新坊弦也、山盛徹、趙松吉、久下祐司、稲波修
  • Organizer: 第152回日本獣医学会学術集会学会
  • Place of Presentation: 大阪府大中百舌鳥キャンパス(大阪)
  • Year and Date: 2011-09-20
• [Presentation] p66shc CH2ドメインを介したタンパク質相互作用が細胞の放射線感受性に与える影響 (2011)
  • Author(s): 山盛徹、松下明子、女池俊介、永瀧正人、安井博宣、稲波修
  • Organizer: 第152回日本獣医学会学術集会学会
  • Place of Presentation: 大阪府大中百舌鳥キャンパス(大阪)
  • Year and Date: 2011-09-20
• [Presentation] マウス扁平上皮癌SCCV II細胞におけるmethyl-pyruvateを用いた放射線増感効果とそのメカニズムの解析 (2011)
  • Author(s): 西田直哉、安井博宣、山盛徹、稲波修
  • Organizer: 第152回日本獣医学会学術集会学会
  • Place of Presentation: 大阪府大中百舌鳥キャンパス(大阪)
  • Year and Date: 2011-09-20
• [Presentation] インビボ電子スピン共鳴法を用いた放射線照射後の再酸素化機構に関する研究 (2011)
  • Author(s): 永根大幹、安井博宣、山盛徹、中村秀夫、稲波修
  • Organizer: 第152回日本獣医学会学術集会学会
  • Place of Presentation: 大阪府大中百舌鳥キャンパス(大阪)
  • Year and Date: 2011-09-20
• [Presentation] Guanine nucleotide exchange factor-H1はMKN45細胞においてビンクリスチンによる浸潤能の促進を媒介する (2011)
  • Author(s): 永瀧正人、山盛徹、女池俊介、安井博宣、稲波修
  • Organizer: 第152回日本獣医学会学術集会学会
  • Place of Presentation: 大阪府大中百舌鳥キャンパス(大阪)
  • Year and Date: 2011-09-19
• [Presentation] Iozing radiation stimulates mitochondrial electron transport chainactivity and increases mitochondrial content (2011)
  • Author(s): Yamamori T, Yasui H, Yamazumi M, Nakamura Y, Nakamura H, Inanami O
  • Organizer: 5th Biennial Meeting of Society for Free Radical Research-Asia, 8th Conference of Asian Society for Mitochondrial Research and Medicine, 11th Conference of Japanese Society of Mitochondrial Research and Medicine
  • Place of Presentation: 鹿児島市民文化ホール(鹿児島)
  • Year and Date: 2011-09-02
• [Presentation] Novel EPR imaging to visualize cycling hypoxia associated with a defect of vascular integrity in transplanted tumors (2011)
  • Author(s): Yasui H, Inanami O, Matsumoto S, J.B.Michell, M.C.Krishna
  • Organizer: 14th International Congress of Radiation Research Incorporating 57^<th> Annual Meeting of the Radiation Research Society
  • Place of Presentation: Memorial to Maria Sklodowska-Curie(ワルシャワ)
  • Year and Date: 2011-08-29
• [Presentation] Novel EPR imaging to visualize cycling hypoxia associated with a defect of vascular integrity integrity in transplanted tumors. (2011)
  • Author(s): 稲波修,安井博宣
  • Organizer: 14th International Congress of Radiation Research
  • Place of Presentation: Warsaw, Memorial to Maria Sklodowska-Curie
  • Year and Date: 2011-08-28
• [Presentation] X線照射により引き起こされるミトコンドリア由来活性酸素種生成メカニズムの解析 (2011)
  • Author(s): 山盛徹、安井博宣、山住雅之、中村吉就、中村秀夫、桑原幹典、稲波修
  • Organizer: 第64回日本酸化ストレス学会
  • Place of Presentation: ルスツリゾートホテル&コンベンション(虻田郡留寿都村)
  • Year and Date: 2011-07-02
• [Presentation] 細胞周期との関連性からみたX線照射に起因する活性酸素種の遅発性産生機構 (2011)
  • Author(s): 安井博宣, 山盛徹, 和田悠佑, 山住雅之, 稲波修
  • Organizer: 第64回日本酸化ストレス学会
  • Place of Presentation: ルスツリゾートホテル&コンベンション(虻田郡留寿都村)
  • Year and Date: 2011-07-02
• [Presentation] Dichloroacetateを用いたがん細胞の代謝調節による放射線増感作用 (2011)
  • Author(s): 安井博宣, 西田直哉, 山盛徹, 稲波修
  • Organizer: 第17回国際癌治療増感研究会
  • Place of Presentation: 岩松旅館(仙台)
  • Year and Date: 2011-06-25
• [Presentation] 間欠的低酸素がラットグリオーマ細胞の放射線感受性に与える影響 (2011)
  • Author(s): 安井博宣、新坊弦也、山盛徹、趙松吉、久下祐司、稲波修
  • Organizer: 第13回癌治療増感研究シンポジウム
  • Place of Presentation: 猿沢荘、京都
  • Year and Date: 2011-02-11
• [Presentation] Ionizing radiation increased mitochondrial electrontrasnsport chain activity and mitochondrial content inhuman lung carcinoma A549 cells (2011)
  • Author(s): Tohoru Yamamori, Hironobu Yasui, Hideo Nakamura, Masayuki Yamazumi, Osamu Inanami
  • Organizer: International Symposium on Free Radical Research
  • Place of Presentation: Hyatt Regency Kyoto 京都
  • Year and Date: 2011-01-21
• [Presentation] X線照射により誘導されるミトコンドリア由来活性酸素種生成上昇メカニズムの解析 (2010)
  • Author(s): 山盛徹、安井博宣、中村秀夫、山住雅之、稲波修
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都テルサ、京都
  • Year and Date: 2010-10-21
• [Presentation] X線照射されたA549細胞における遅発性細胞内活性酸素種産生と細胞周期との関連性 (2010)
  • Author(s): 和田悠祐、山盛徹、安井博宣、山住雅之、稲波修
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都テルサ、京都
  • Year and Date: 2010-10-21
• [Presentation] 核酸代謝拮抗剤TAS106による相同組換え修復阻害作用を介した放射線増感効果 (2010)
  • Author(s): 女池俊介、山盛徹、安井博宣、松田彰、森松正美、福島正和、山崎靖人、稲波修
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都テルサ、京都
  • Year and Date: 2010-10-20
• [Presentation] 間欠的低酸素が腫瘍内の放射線抵抗性に与える影響 (2010)
  • Author(s): 新坊弦也、安井博宣、山盛徹、稲波修
  • Organizer: 日本放射線影響学会第53回大会
  • Place of Presentation: 京都テルサ、京都
  • Year and Date: 2010-10-20
• [Presentation] Identification of a novel Cu (II) binding site in full-length murine prionprotein by using ESR/SDSL technique. (2010)
  • Author(s): O.Inanami, Y.Watanabe, M.Igarashi, K.Ito, T.Yamamori, Hyasui, W.Hiraoka
  • Organizer: Asia-Pacific EPR/ESR Symposium2010
  • Place of Presentation: International Convention Center、済州(韓国)
  • Year and Date: 2010-10-12
• [Presentation] 高濃度VincristineはROCK経路活性化を介してアメーバ様浸潤を誘導する (2010)
  • Author(s): 永瀧正人、山盛徹、稲波修
  • Organizer: 第150回日本獣医学会学術集会
  • Place of Presentation: 帯広畜産大学、帯広
  • Year and Date: 2010-09-17
• [Presentation] X線照射後に遅れて起きる細胞内活性酸素種の増加反応 (2010)
  • Author(s): 稲波修、安井博宣、山住雅之、桑原幹典、山盛徹
  • Organizer: 第63回日本酸化ストレス学会学術集会
  • Place of Presentation: 神奈川県民ホール、横浜
  • Year and Date: 2010-06-24
• [Presentation] 新規抗癌剤と放射線併用によるG_2チェックポイントとアポトーシス誘導 (2010)
  • Author(s): 稲波修
  • Organizer: 第12回 癌治療増感研究シンポジウム 4つの"R"-現象論から分子メカニズムの解明へ-
  • Place of Presentation: 奈良
  • Year and Date: 2010-02-13
• [Presentation] Idenfication of a novel Cu (II) binding site in full-length murine prionprotein by using ESR/SDSL technique (2010)
  • Author(s): 稲波修
  • Organizer: Asia-Pacific EPR/ESR Symposium
  • Place of Presentation: Jeju
• [Presentation] マウス扁平上皮がんSCC VII細胞におけるエネルギー代謝と放射線感受性 (2009)
  • Author(s): 西田直哉、山盛徹、安井博宣、稲波修
  • Organizer: 第52回 日本放射線影響学会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-11
• [Presentation] X線照射はA549細胞においてミトコンドリア機能亢進と遅発性細胞内活性酸素種産生を誘導する (2009)
  • Author(s): 山住雅之、山盛徹、稲波修
  • Organizer: 第52回 日本放射線影響学会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-11
• [Presentation] 金粒子含有ナノゲル存在下でのX線照射による細胞死の増強作用とその機序の解析 (2009)
  • Author(s): 武内亮、安井博宣、山盛徹、中村隆仁、大石基、長崎幸夫、稲波修
  • Organizer: 第52回 日本放射線影響学会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-11
• [Presentation] 8-Amino-adenosineによる放射線誘導細胞死の増感効果 (2009)
  • Author(s): 女池俊介、山盛徹、安井博信、松田彰、稲波修
  • Organizer: 第52回 日本放射線影響学会
  • Place of Presentation: 広島
  • Year and Date: 2009-11-11
• [Presentation] ヒト胃腺がん細胞MKN45における浸潤能と微小管重合状態の関連性 (2009)
  • Author(s): 永瀧正人、山盛徹、稲波修
  • Organizer: 第82回 日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] マウスプリオンタンパク質ヒスジン186周辺の銅イオン結合部位に関する構造解析 (2009)
  • Author(s): 稲波修、渡辺康子、五十嵐学、伊藤公人、堀内基広、桑原幹典、平岡和佳子
  • Organizer: 第148回 日本獣医学会学術集会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-09-25
• [Presentation] A549細胞における8-amino-adenosineと放射線の併用による抗腫瘍効果の検討 (2009)
  • Author(s): 女池俊介、山盛徹、安井博信、松田彰、稲波修
  • Organizer: 第148回 日本獣医学会学術集会
  • Place of Presentation: 鳥取
  • Year and Date: 2009-09-25
• [Presentation] 腫瘍細胞における金粒子含有ナノゲルを用いた放射線増感作用 (2009)
  • Author(s): 武内亮、安井博宣、山盛徹、中村隆仁、大石基、長崎幸夫、稲波修
  • Organizer: 第48回 日本医学放射線学会生物部会学術大会
  • Place of Presentation: 富山
  • Year and Date: 2009-07-11
• [Presentation] ヌクレオシド誘導体による放射線増感 (2009)
  • Author(s): 稲波修
  • Organizer: 第39回 放射線による制癌シンポジウム-基礎と臨床との対話-
  • Place of Presentation: 富山
  • Year and Date: 2009-07-10
• [Presentation] 制癌剤処理細胞における細胞内活性酸素種の増加と浸潤能力との関連性について (2009)
  • Author(s): 永瀧正人、山盛徹、稲波修
  • Organizer: 第62回 日本酸化ストレス学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2009-06-11
• [Presentation] 放射線照射したA549細胞に見られる遅延性細胞内活性酸素種の上昇に関する研究 (2009)
  • Author(s): 山住雅之、山盛徹、稲波修
  • Organizer: 第62回 日本酸化ストレス学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2009-06-11
• [Presentation] 経口吸着薬AST-120(クレメジン^<[○!R]>)は虚血性急性腎不全(ARF)ラット腎ミトコドリアからのフリーラジカル産生を抑制する (2009)
  • Author(s): 大和田滋、平山暁、植田敦志、伊藤俊輔、後藤寿美恵、西島冬彦、稲波修
  • Organizer: 第62回 日本酸化ストレス学会学術集会
  • Place of Presentation: 福岡
  • Year and Date: 2009-06-11
• [Presentation] マウスプリオンタンパク質ヒスチジン186周辺の銅イオン結合部位に関する構造解析 (2009)
  • Author(s): 稲波修
  • Organizer: 第148回日本獣医学会
  • Place of Presentation: 鳥取
• [Remarks]
  • URL: http://www.vetmed.hokudai.ac.jp/organization/radbiol/index.html
• [Remarks]
  • URL: http://www.vetmed.hokudai.ac.jp/organization/radbiol/index.html
• [Remarks]
  • URL: http://www.vetmed.hokudai.ac.ju/organization/radbiol/index.html
• [Remarks]
  • URL: http://www.vetmed.hokudai.ac.jp/organization/radbiol/index.html