Basic study on mechanisms of metacyclogenesis-related vector-parasite relationships

• Project/Area Number: 21380186
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied veterinary science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: INOUE Noboru 帯広畜産大学, 原虫病研究センター, 教授 (10271751)
• Co-Investigator(Kenkyū-buntansha): KAWAZU Shin-ichiro 帯広畜産大学, 原虫病研究センター, 教授 (60312295) ; GOTO Yasuyuki 東京大学, 大学院・農学生命科学研究科, 准教授 (50553434)
• Co-Investigator(Renkei-kenkyūsha): SUGIMOTO Chihiro 北海道大学, 人獣共通感染症リサーチセンター, 教授 (90231373) ; WATANABE Jyunichi 東京大学, 医科学研究所, 助教 (20201189) ; KURATA Shouichiro 東北大学, 薬学研究科, 教授 (90221944)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000); Fiscal Year 2011: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000); Fiscal Year 2010: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
• Keywords: 疾病予防・制御 / ツェツェバエ / アフリカトリパノソーマ / 生活環 / 遺伝子転写翻訳制御 / ライフサイクル / 細胞分化 / 遺伝子 / 転写翻訳制御 / 感染症 / 昆虫 / 原虫 / ツエツエバエ

Research Abstract

The world's first whole life-cycle stage proteome and EST data bases were established. These databases could potentially promote basic studies on control measures of human and animal African trypanosomoses. In addition, identification, cloning and characterization of congolense epimastigote specific protein (CESP) provided a novel knowledge which enhances further studies on trypanosome control strategies in its vector insect, tsetse.

Research Products

• [Journal Article] アフリカトリパノソーマの遺伝子発現調節機構 (2013)
  • Author(s): 菅沼啓輔、井上昇
  • Journal Title: 獣医寄生虫学会誌 Volume: 11 Pages: 87-92
  • Peer Reviewed
• [Journal Article] The epimastigote stage-specific gene expression of CESP is tightly regulated by its 3'UTR (2012)
  • Author(s): Suganuma, K., Yamasaki, S., Asada, M., Kawazu, S. and Inoue, N.
  • Journal Title: Molecular and Biochemical Parasitology Volume: 186 (1) Issue: 1 Pages: 77-80
  • DOI: 10.1016/j.molbiopara.2012.09.010
  • Peer Reviewed
• [Journal Article] Kinetics of regulatory dendritic cells in inflammatory responses during Trypanosoma evansi infection (2012)
  • Author(s): Hirohisa Mekata
  • Journal Title: Parasite Immunology Volume: 34 Issue: 6 Pages: 318-329
  • DOI: 10.1111/j.1365-3024.2012.01362.x
  • Peer Reviewed
• [Journal Article] Structural characterization and epitope mapping of the Glutamic Acid/Alanine Rich protein from Trypanosoma congolense : Defining assembly on the parasite cell surface (2011)
  • Author(s): Loveless, B. C., Mason, J.W., Sakurai, T., Inoue, N., Razavi, M., Pearson, T. W. and Boulanger, M. J.
  • Journal Title: The Journal of Biological Chemistry Volume: 286(23) Issue: 23 Pages: 20658-20665
  • DOI: 10.1074/jbc.m111.218941
  • Peer Reviewed
• [Journal Article] Differential protein expression throughout the life cycle of Trypanosoma congolense, a major parasite of cattle in Africa (2011)
  • Author(s): Eyford, B. A., Sakurai, T., Smith, D., Loveless, B. C., Hertz-Fowler, C., Donelson, J. E., Inoue, N. and Pearson, T. W.
  • Journal Title: Molecular & Biochemical Parasitology Volume: 177 (2) Issue: 2 Pages: 116-125
  • DOI: 10.1016/j.molbiopara.2011.02.009
  • Peer Reviewed
• [Journal Article] Biased cellular locations of tandem repeat antigens in African trypanosomes (2011)
  • Author(s): Goto, Y., Duthie, M. S., Kawazu, S., Inoue, N. and Carter, D.
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 405 (3) Issue: 3 Pages: 434-438
  • DOI: 10.1016/j.bbrc.2011.01.048
  • Peer Reviewed
• [Journal Article] Structural characterization and epitope mapping of the Glutamic Acid/A lanine Rich protein from Trypanosoma congolense : Defining assembly on the parasite cell surfac (2011)
  • Author(s): Loveless BC, et al.
  • Journal Title: The Journal of Biological Chemistry Volume: (印刷中)
  • Peer Reviewed
• [Journal Article] Differential protein expression throughout the life cycle of Trypanosoma congolense, a major parasite of cattle in Africa (2011)
  • Author(s): Eyford BA, et al.
  • Journal Title: Molecular & Biochemical Parasitology Volume: 177 Pages: 116-125
  • Peer Reviewed
• [Journal Article] Trypanosome alternative oxidase, a potential therapeutic target for sleeping sickness, is conserved among Trypanosoma brucei subspecies (2010)
  • Author(s): Nakamura, K., Fujioka, S., Fukumoto, S., Inoue, N., Sakamoto, K., Hirata, H., Kido, Y., Yabu, Y., Suzuki, T., Watanabe, Y., Saimoto, H., Akiyama, H. and Kita, K.
  • Journal Title: Parasitology International Volume: 59 (4) Issue: 4 Pages: 560-564
  • DOI: 10.1016/j.parint.2010.07.006
  • NAID: 10029826326
  • Peer Reviewed
• [Journal Article] Upregulated expression of B-cell antigen family tandem repeat proteins by Leishmania amastigotes (2010)
  • Author(s): Goto, Y., Carter, D., Guderian, J., Inoue, N., Kawazu, S. and Reed, S. G.
  • Journal Title: Infection and Immunity Volume: 78 (5) Issue: 5 Pages: 2138-2145
  • DOI: 10.1128/iai.01102-09
  • Peer Reviewed
• [Journal Article] Analysis of expressed sequence tags from the four main developmental stages of Trypanosoma congolense (2009)
  • Author(s): Helm, J. R., Hertz-Fowler, C., Aslett, M., Berriman, M., Sanders, M., Quail, M. A., Soares, M. B., Bonaldo, M. F., Sakurai, T., Inoue, N. and Donelson, J. E.
  • Journal Title: Molecular and Biochemical Parasitology Volume: 168 (1) Issue: 1 Pages: 34-42
  • DOI: 10.1016/j.molbiopara.2009.06.004
  • Peer Reviewed
• [Journal Article] Analysis of expressed sequence tags from the four main developmental stages of Trypanosoma congolense. (2009)
  • Author(s): Helm, J.R., et al.
  • Journal Title: Molecular and Biochemical Parasitology 168 Pages: 34-42
  • Peer Reviewed
• [Presentation] Analysis of the cis-element for gene expression regulation in congolense epimastigote specific protein (CESP) (2013)
  • Author(s): Suganuma, K.
  • Organizer: The 24^<th> International Conference of the World Association for the Advancement of Veterinary Parasitology
  • Place of Presentation: パース・オーストラリア
• [Presentation] Trypanosoma congolense EMFステージ特異的遺伝子の発現調節機構 (2013)
  • Author(s): 菅沼啓輔
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京
• [Presentation] Trypanosoma congolense EMF特異的遺伝子cespの発現調節領域の特定 (2013)
  • Author(s): 菅沼啓輔
  • Organizer: 第20回分子寄生虫ワークショップ
  • Place of Presentation: 神戸
• [Presentation] Analysis of the cis-element for gene expression regulation in congolense epimastigote specific protein (CESP) (2013)
  • Author(s): Keisuke Suganuma
  • Organizer: The 24th International Conference of the World Association for the Advancement of Veterinary Parasitology
  • Place of Presentation: パース、オーストラリア
• [Presentation] アフリカトリパノソーマ細胞表面分子による宿主認識とトロピズム (2011)
  • Author(s): 井上昇
  • Organizer: 第4回原虫感染免疫研究会
  • Place of Presentation: 長崎
  • Year and Date: 2011-02-04
• [Presentation] アフリカトリパノソーマ細胞表面分子による宿主認識とトロピズム (2011)
  • Author(s): 井上昇
  • Organizer: 第四回原虫感染免疫研究会
  • Place of Presentation: 長崎大学医学部
  • Year and Date: 2011-02-04
• [Presentation] 新たに同定したアフリカトリパノソーマのツェツェバエ体内ステージ特異的表面分子CESPについて (2010)
  • Author(s): 井上昇
  • Organizer: 第63回日本寄生虫学会南日本支部会・第60回日本衛生動物学会南日本支部会合同大会
  • Place of Presentation: 鹿児島
  • Year and Date: 2010-11-06
• [Presentation] 新たに同定したアフリカトリパノソーマのツェツェバエ体内ステージ特異的表面分子CESPについて (2010)
  • Author(s): 井上昇
  • Organizer: 第63回日本寄生虫学会南日本支部大会・第60回日本衛生動物学会南日本支部大会合同大会
  • Place of Presentation: 鹿児島大学農学部
  • Year and Date: 2010-11-06
• [Presentation] Trypanosoma congolense EMF虫体特異的表面蛋白(CESP)の多型解析 (2009)
  • Author(s): 竹本知代
  • Organizer: 第17回分子寄生虫ワークショップ
  • Place of Presentation: 群馬
• [Presentation] Trypanosoma congolense (Tc)のツェツェ体内におけるCESP発現パターンの解析 (2009)
  • Author(s): 菅沼啓輔
  • Organizer: 第17回分子寄生虫ワークショップ
  • Place of Presentation: 群馬
• [Presentation] Trypanosoma congolense CESPの多型解析 (2009)
  • Author(s): 竹本知代
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取
• [Presentation] Trypanosoma congolense (Tc)のツェツェバエ体内におけるCESP発現パターンの解析 (2009)
  • Author(s): 菅沼啓輔
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取
• [Presentation] Trypanosoma congolense EMF虫体特異的表面蛋白(CESP)の多型解析 (2009)
  • Author(s): 竹本知代
  • Organizer: 第17回分子寄生虫ワークショップ
  • Place of Presentation: 群馬県吾妻郡草津町
• [Presentation] Trypanosoma congolense(Tc)のツエツエ体内におけるCESP発現パターンの解析 (2009)
  • Author(s): 菅沼啓輔
  • Organizer: 第17回分子寄生虫ワークショップ
  • Place of Presentation: 群馬県吾妻郡草津町
• [Presentation] Trypanosoma congolense CESPの多型解析 (2009)
  • Author(s): 竹本知代
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取県鳥取市
• [Presentation] Trypanosoma congolense(Tc)のツエツエバエ体内におけるCESP発現パターンの解析 (2009)
  • Author(s): 菅沼啓輔
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取県鳥取市
• [Book] 改訂版人獣共通感染症 (2011)
  • Author(s): 井上昇(分担執筆)
  • Publisher: 医薬ジャーナル社
• [Book] 動物の感染症第三版 (2011)
  • Author(s): 井上昇(分担執筆)
  • Total Pages: 144
  • Publisher: 近代出版
• [Book] 改訂版人獣嫡感染症木村哲・喜田宏編 (2011)
  • Author(s): 井上昇(分担執筆)
  • Total Pages: 551
  • Publisher: 医薬ジャーナル社
• [Book] 動物の感染症小沼操ほか編 (2011)
  • Author(s): 井上昇(分担執筆)
  • Publisher: 近代出版(未定)
• [Remarks]
  • URL: http://www.obihiro.ac.jp/~tryp
• [Remarks]
  • URL: http://www.obihiro.ac.jp/~tryp