Clarification of molecular basis of transporter diseases by the comprehensive detection of protein-rotein interactions.
| Project/Area Number |
21380191
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Clinical veterinary science
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| Research Institution | Hokkaido University |
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Principal Investigator |
SATO Kota 北海道大学, 大学院・獣医学研究科, 准教授 (50283974)
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| Co-Investigator(Kenkyū-buntansha) |
INABA Mutsumi 北海道大学, 大学院・獣医学研究科, 教授 (00183179)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000)
Fiscal Year 2011: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
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| Keywords | トランスポーター / 蛋白質問相互作用 / アダプター蛋白質 / チャネル / 蛋白質間相互作用 / 生体膜 / RNAi |
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| Research Abstract |
Transporter diseases are caused by disorders of various membrane transporter and channel proteins. We analyzed the comprehensive protein-protein interactions that alter intracellular traffic of transporters by utilizing the hereditary defects of glutamate transporter GLAST in Japanese dog red cells as a model of the transporter disease. The PDZ domain proteins NHERF1 and NHERF2 interacted with the GLAST proteins in the endoplasmic reticulum and the post-Golgi compartments during the traffic to the plasma membrane, respectively. Since coexpression of these proteins altered the cell surface expression level of GLAST, it is suggested that the interaction of PDZ domain proteins with GLAST is associated with the disorder of intracellular trafficking of GLAST.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Extrusion of Na, K-ATPase and transferring receptor with lipid raft-associated proteins in different populations of exosomes during reticulocyte maturation in dogs2010
Author(s)
Komatsu, T., Arashiki, N., Otsuka, Y., Sato, K., Inaba, M
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Journal Title
Jpn. J. Vet. Res.
Volume: 58
Pages: 17-27
URL
Related Report
Peer Reviewed
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[Journal Article] Parallel reductions in stomatin and Na,K-ATPase through the exosomal pathway during reticulocyte maturation in dogs:stomatin as a genotypic and phenotypic marker of high K^+ and low K^+ red cells.2010
Author(s)
Komatsu, T., Sato, K., Otsuka, Y., Arashiki, N., Tanaka, K., Tamahara, S., Ono, K., Inaba, M.
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Journal Title
J. Vet. Med. Sci
Volume: 72
Pages: 893-901
URL
Related Report
Peer Reviewed
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