Molecular analysis of resistant mechanisms against both hypoxic and pro-apoptotic stimulation in malignant hematopoietic cell tumors.

• Project/Area Number: 21380194
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Clinical veterinary science
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: TANAKA Akane 東京農工大学, 大学院・農学研究院, 教授 (80418673)
• Co-Investigator(Kenkyū-buntansha): MATSUDA Hiroshi 東京農工大学, 大学院・農学研究院, 教授 (80145820) ; OHMORI Keitaro 東京農工大学, 大学院・農学研究院, 講師 (20466915)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,630,000 (Direct Cost: ¥15,100,000、Indirect Cost: ¥4,530,000); Fiscal Year 2011: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2010: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2009: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
• Keywords: 肥満細胞腫 / リンパ腫 / 白血病 / 伴侶動物 / 細胞増殖

Research Abstract

he malignant acquired character that is the most serious in the tumor development of the cell is the abnormal proliferation and apoptosis resistance by the disablement of the cell death signal. In this study, I analyzed the disablement mechanism of the endogenous apoptotic process dependent on the mitochondria in the haematopoietic malignant tumor and cell function maintenance mechanism under the hypoxia environment. I also searched for the molecular target medicine which showed anti-cancer property. The crosstalk of the hypoxia-inducible factor(HIF) and NF-κB resulting in apoptosis resistance was investigated.

Research Products

• [Journal Article] Peroxisome proliferator-activated receptorγ-mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC/Tnd mice (2011)
  • Author(s): Jung K, Tanaka A, Fujita H, Matsuda A, Oida K, Karasawa K, Okamoto N, Ohmori K, Jee Y, Shin T, Matsuda H.
  • Journal Title: J Allergy Clin Immunol Volume: 127 Issue: 2 Pages: 420-429
  • DOI: 10.1016/j.jaci.2010.10.043
  • Peer Reviewed
• [Journal Article] Silencing of Int6 gene restores function of the ischemic hindlimb in a rat model for peripheral arterial disease (2011)
  • Author(s): Okamoto N, Tanaka A, Jung K, Karasawa K, Orito K, Matsuda A, Amagai Y, Oida K, Ohmori K, Matsuda H.
  • Journal Title: diovasc Res Volume: 92 Issue: 2 Pages: 209-217
  • DOI: 10.1093/cvr/cvr203
  • Peer Reviewed
• [Journal Article] Glucocorticoid sensitivity depends on expression levels of glucocorticoid receptors in canine neoplastic mast cells (2011)
  • Author(s): Matsuda A, Tanaka A, Amagai Y, Ohmori K, Nishikawa S, Xia Y, Karasawa K, Okamoto N, Oida K, Jang H, Matsuda H.
  • Journal Title: Vet Immunol Immunopathol Volume: 144 Issue: 3-4 Pages: 321-328
  • DOI: 10.1016/j.vetimm.2011.08.013
  • Peer Reviewed
• [Journal Article] Consecutive daily low-dose S-1 adjuvant chemotherapy after radical treatment for squamous cell carcinoma in head and neck cancer (2011)
  • Author(s): Furusaka T, Tanaka A, Matsuda H, Ikeda M.
  • Journal Title: Acta Otolaryngol Volume: 131 Issue: 10 Pages: 1099-1103
  • DOI: 10.3109/00016489.2011.590153
  • Peer Reviewed
• [Journal Article] Mast cells function as an alternative modulator of adipogenesis through 15-deoxy-delta-12, 14-prostaglandin J2 (2011)
  • Author(s): Tanaka A, Nomura Y, Matsuda A, Ohmori K, Matsuda H.
  • Journal Title: Am J Physiol Cell Physiol Volume: 301 Issue: 6 Pages: 1360-1367
  • DOI: 10.1152/ajpcell.00514.2010
  • Peer Reviewed
• [Journal Article] Mast cells function as an alternative modulator of adipogenesis through 15-deoxy-delta-12,14-prostaglandin J_2 (2011)
  • Author(s): Tanaka A, Nomura Y, Matsuda A, Ohmori K, Matsuda H
  • Journal Title: Am.J.Physiol.Cell Physiol Volume: 301 Pages: 360-367
  • Peer Reviewed
• [Journal Article] Glucocorticoid sensitivity depends on expression levels of glucocorticoid receptors in canine neoplastic mast cells (2011)
  • Author(s): Matsuda A, Tanaka A, Amagai Y, Ohmori K, Nishikawa S, Xia Y, Karasawa K, Okamoto N, Oida K, Jang H, Matsuda H
  • Journal Title: Vet.Immunol.Immunopathol. Volume: 144 Pages: 321-328
  • Peer Reviewed
• [Journal Article] Silencing of Int6 gene restores function of the ischemic hindlimb in a rat model for peripheral arterial disease (2011)
  • Author(s): Okamoto N, Tanaka A, Jung K, Karasawa K, Orito K, Matsuda A, Amagai Y, Oida K, Ohmori K, Matsuda H
  • Journal Title: Cardiovasc.Res. Volume: 92 Pages: 209-217
  • Peer Reviewed
• [Journal Article] Peroxisome proliferator-activated receptor γ-mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC/Tnd mice. (2011)
  • Author(s): Jung K, Tanaka A, Fujita H, Matsuda A, Oida K, Karasawa K, Okamoto N, Ohmori K, Jee Y, Shin T, Matsuda H.
  • Journal Title: Journal of Allergy and Clinical Immunology Volume: 127 Pages: 420-429
  • Peer Reviewed
• [Journal Article] Evaluation of itch by using NC/NgaTnd mice : a model of human atopic dermatitis. (2011)
  • Author(s): Tanaka A, Matsuda H.
  • Journal Title: Journal of Biomedicine and Biotechnology Volume: 790436
  • Peer Reviewed
• [Journal Article] A novel NF-kappa B inhibitor improves glucocorticoid sensitivity of canine neoplastic lymphoid cells by up-regulating expression of glucocorticoid receptors (2010)
  • Author(s): Matsuda A, Tanaka A, Muto S, Ohmori K, Furusaka T, Jung K, Karasawa K, Okamoto N, Oida K, Itai A, Matsuda H.
  • Journal Title: Res Vet Sci Volume: 89 Pages: 378-382
  • Peer Reviewed
• [Journal Article] Pilot evaluation of the efficacy of shampoo treatment with ultra-pure soft water for canine pruritus (2010)
  • Author(s): Ohmori K, Tanaka A, Makita Y, Takai M, Yoshinari, Y, Matsuda H.
  • Journal Title: Vet Dermatol Volume: 21 Issue: 5 Pages: 477-483
  • DOI: 10.1111/j.1365-3164.2010.00900.x
  • Peer Reviewed
• [Journal Article] A novel NF-κB inhibitor improves glucocorticoid sensitivity of canine neoplastic lymphoid cells by up-regulating expression of glucocorticoid receptors. (2010)
  • Author(s): Matsuda A, Tanaka A, Muto S, Ohmori K, Furusaka T, Jung K, Karasawa K, Okamoto N, Oida K, Itai A, Matsuda H.
  • Journal Title: Research in Veterinary Science Volume: 89 Pages: 387-382
  • Peer Reviewed
• [Journal Article] A novel NF-kappaB inhibitor improves glucocorticoid sensitivity of canine neoplastic lymphoid cells by up-regulating expression of glucocorticoid receptors (2010)
  • Author(s): 松田彬, 田中あかね, Muto S, 大森啓太郎, Furusaka T, Jung K, Karasawa K, Okamoto N, Oida K, Itai A, 松田浩珍
  • Journal Title: Research in Veterinary Science (In press)
  • Peer Reviewed
• [Journal Article] Pilot evaluation of the efficacy of shampoo treatment with ultra-pure soft water for canine pruritus (2010)
  • Author(s): 大森啓太郎, 田中あかね, Makita Y, Takai M, Yoshinari, Y, 松田浩珍
  • Journal Title: Veterinary Dermatology (In press)
  • Peer Reviewed
• [Journal Article] Oral supplementation with Lactobacillus rhamnosus CGMCC 1. 3724 prevents development of atopic dermatitis in NC/NgaTnd mice possibly by modulating local production of IFN-gamma (2009)
  • Author(s): Tanaka A, Jung K, Benyacoub J, Prioult G, Okamoto N, Ohmori K, Blum S, Mercenier A, Matsuda H.
  • Journal Title: Exp Dermatol Volume: 18 Pages: 1022-1027
  • Peer Reviewed
• [Journal Article] Oral supplementation with Lactobacillus rhamnosus CGMCC 1.3724 prevents development of atopic dermatitis in NC/NgaTnd mice possibly by modulating local production of IFN-gamma (2009)
  • Author(s): 田中あかね, Jung K, Benyacoub J, Prioult G, Okamoto N, 大森啓太郎, Blum S, Mercenier A, 松田浩珍
  • Journal Title: Experimental Dermatology 18(12) Pages: 1022-1027
  • Peer Reviewed
• [Presentation] XXII World Allergy Congress (2011)
  • Organizer: Metallic soap aggravates skin conditions in patients with atopic dermatitis and a mouse model for human atopic dermatitis
  • Place of Presentation: メキシコ・カンクン
  • Year and Date: 2011-12-09
• [Presentation] Meitallic soap aggravates skin conditions in patients with atopic dermatitis and a mouse model for human atopic dermatitis, NC/Tnd mice (2011)
  • Author(s): Tanaka A.
  • Organizer: XXII World Allergy Congress
  • Place of Presentation: メキシコ・カンクン
  • Year and Date: 2011-12-09
• [Presentation] Expression levels of glucocorticoid receptors correlated with glucocorticoid sensitivity in mast cell tumors (2011)
  • Organizer: 1st Biohousing International Symposium
  • Place of Presentation: 韓国・グアンジュ
  • Year and Date: 2011-09-30
• [Presentation] Expression levels of glucocorticoid receptors correlated with glucocorticoid sensitivity in mast cell tumors (2011)
  • Author(s): Tanaka A.
  • Organizer: 1st Biohousing International Symposium
  • Place of Presentation: 韓国・グアンジュ(招待講演)
  • Year and Date: 2011-09-30
• [Presentation] Expression levels of glucocorticoid receptors correlated with glucocorticoid sensitivity in mast cell tumors (2011)
  • Organizer: 22^<nd> World Congress of Dermatology
  • Place of Presentation: 韓国・ソウル
  • Year and Date: 2011-05-28
• [Presentation] Expression levels of glucocorticoid receptors correlated with glucocorticoid sensitivity in mast cell tumors (2011)
  • Author(s): Tanaka A.
  • Organizer: 22^<nd> World Congress of Dermatology
  • Place of Presentation: 韓国・ソウル(招待講演)
  • Year and Date: 2011-05-28
• [Presentation] PPARγ-mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC/NgaTnd mice (2010)
  • Organizer: Skin Allergy Meeting
  • Place of Presentation: イタリア・ベニス
  • Year and Date: 2010-11-12
• [Presentation] PPARy-mediated suppression of dendritic cell function prevents the onset of atopic dermatitis in NC/NgaTnd mice. (2010)
  • Author(s): Tanaka A, Jung K, Ohmori K, Matsuda H.
  • Organizer: Skin Allergy Meeting 2010
  • Place of Presentation: イタリア・ベニス
  • Year and Date: 2010-11-12
• [Presentation] リンパ腫/白血病細胞におけるグルココルチコイド耐性獲得機構における転写因子NF-κBの関与 (2009)
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取県
  • Year and Date: 2009-09-26
• [Presentation] ヒト乳癌細胞株の生存・増殖におけるNF-κBの役割 (2009)
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取県鳥取市
  • Year and Date: 2009-09-26
• [Presentation] リンパ腫/白血病細胞におけるグルココルチコイド耐性獲得機構における転写因子NF-KBの関与 (2009)
  • Author(s): 松田彬、大森啓太郎、田中あかね、松田浩珍
  • Organizer: 第148回日本獣医学会学術集会
  • Place of Presentation: 鳥取県鳥取市
  • Year and Date: 2009-09-26
• [Presentation] 肥満細胞腫におけるアポトーシス耐性獲得機構の解析 (2009)
  • Organizer: 第147回日本獣医学会学術集会
  • Place of Presentation: 栃木県宇都宮市
  • Year and Date: 2009-04-04
• [Presentation] 肥満細胞腫におけるアポトーシス耐性獲得機構の解析 (2009)
  • Author(s): 松田彬、田中あかね、大森啓太郎、松田浩珍
  • Organizer: 第148回日本獣医学会学術集会 臨床分科会シンポジウム
  • Place of Presentation: 栃木県宇都宮市
  • Year and Date: 2009-04-04
• [Book] 疾患モデルマウス表現型解析指南(アレルギー別冊、山村研一・若菜茂晴編集) (2011)
  • Author(s): 田中あかね・松田浩珍
  • Total Pages: 400
  • Publisher: 中山書店
• [Book] 疾患モデルマウス表現型解析指南
  • Author(s): 山村研一・若菜茂晴編集
  • Publisher: 中山書店
• [Remarks]
  • URL: http://www.tuat.ac.jp/~mol_path/
• [Remarks]
  • URL: http://www.tuat.ac.jp/~mol_path/
• [Remarks] 東京農工大学・獣医分子病態治療学研究室ホームベージ
  • URL: http://www.tuat.ac.jp/~mol_path/index.html
• [Remarks]
  • URL: http://www.tuat.ac.jp/~mol_path/index.html
• [Patent(Industrial Property Rights)] 移植用細胞シートの製造方法、移植用細胞シート、及び移植用細胞シートを用いる (2012)
  • Inventor(s): 田中あかね、松田浩珍
  • Industrial Property Rights Holder: 田中あかね、松田浩珍
  • Filing Date: 2012-08-30
• [Patent(Industrial Property Rights)] オリゴヌクレオチド (2011)
  • Inventor(s): 田中あかね
  • Industrial Property Rights Holder: 田中あかね
  • Industrial Property Number: 2011-274897
  • Filing Date: 2011-12-16
• [Patent(Industrial Property Rights)] オリゴヌクレオチド (2010)
  • Inventor(s): 田中あかね、松田浩珍、松田彬
  • Industrial Property Rights Holder: 田中あかね、松田浩珍、松田彬
  • Filing Date: 2010-12-16
• [Patent(Industrial Property Rights)] 移植用細胞シートの製造方法、移植用細胞シート、及び移植用細胞シートを用いる治療方法 (2010)
  • Inventor(s): 田中あかね、松田浩珍
  • Industrial Property Rights Holder: 田中あかね
  • Filing Date: 2010-08-30