Development of the methods for mass propagation and production of sterile strains of fast growing timber tree crop of Acacia.

• Project/Area Number: 21380196
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Chiba University
• Principal Investigator: MII Masahiro 千葉大学, 大学院・園芸学研究科, 教授 (30093074)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000); Fiscal Year 2012: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000); Fiscal Year 2011: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2009: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
• Keywords: 林木 / バイオマス / 形質転換 / 大量増殖 / 組織培養 / 遺伝子組換え / 染色体倍加 / 熱帯林再生 / 育種学 / 環境 / バイオテクノロジー / 林学

Research Abstract

Efficient method for micropropagation of Acacia mangium and its interspecific hybrids with A. auriculiformis was established through callus and multiple shoot formation. By utilizing this system, transgenic callus and shoot primordia were obtained through Agrobacterium-mediated genetic transformation method. Tetraploid plants were also obtained by treating axillary buds of in vitro propagated plantlets with some chromosome doubling agents.