Studies on structure and function of bacterial yellow affinity substance capable of enhancing enzymatic degradation of cellulose and its gene

• Project/Area Number: 21380197
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Boundary agriculture
• Research Institution: Mie University
• Principal Investigator: SAKKA Kazuo 三重大学, 大学院・生物資源学研究科, 教授 (20154031)
• Co-Investigator(Kenkyū-buntansha): SAKKA Makiko 三重大学, 大学院・生物資源学研究科 (00422882)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2009: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: Clostridium thermocellum / 黄色色素 / セルラーゼ / セルロソーム / セルロース分解

Research Abstract

Yellow affinity substance(YAS) extracted from cells of Clostridium thermocellum strain ATCC 27405 using acetone as the extracting solvent was adsorbed to microcrystalline cellulose(Funacel) and its digestibility was examined using some cellulase preparations. YAS-adsorbed Funacel was about 3-folds more efficiently degraded by cellulosome prepared from the culture supernatant of C. thermocellum ATCC 27405 than non-treated Funacel was. In addition, YAS-adsorbed Funacel was 2.5-folds more susceptible to mini-cellulosome constructed from three cellulases and a chimeric scaffolding protein than non-treated Funacel. This is the first study showing that YAS has an ability to enhance susceptibility of cellulose to cellulolytic enzymes.

Research Products

• [Journal Article] Analysis of cohesin-dockerin interactions using mutant dockerin proteins (2011)
  • Author(s): Kazutaka Sakka, Yuka Sugihara, Sadanari Jindou, Makiko Sakka, Minoru Inagaki, Kazuo Sakka and Tetsuya Kimura
  • Journal Title: FEMS Microbiol Lett Volume: vol.314 Pages: 75-80
  • Peer Reviewed
• [Journal Article] Analysis of cohesin-dockerin interactions using mutant dockerin proteins (2011)
  • Author(s): K.Sakka, 他
  • Journal Title: FEMS Microbiol., Lett. Volume: 314 Pages: 75-80
  • Peer Reviewed
• [Journal Article] Unusual binding properties of the dockerin module of Clostridium thermocellum endoglucanase CelJ(Cel9D-Cel44A) (2009)
  • Author(s): Kazutaka Sakka, Yuko Kishino, Yuka Sugihara, Sadanari Jindou, Makiko Sakka, Minoru Inagaki, Tetsuya Kimura and Kazuo Sakka
  • Journal Title: FEMS Microbiol Lett Volume: vol.300 Pages: 249-255
  • Peer Reviewed
• [Journal Article] Unusual binding properties of the dockerin module of Clostridium thermocellum endoglucanase CelJ (Cel9D-Cel44A) (2009)
  • Author(s): K.Sakka, 他
  • Journal Title: FEMS Microbiol., Lett. 300 Pages: 249-255
  • Peer Reviewed
• [Presentation] 人工ミニセルロソームにおけるファミリー3のCBMの機能解析 (2011)
  • Author(s): 吉田和生、粟冠真紀子、木村哲哉、粟冠和郎
  • Organizer: セルラーゼ研究会
  • Place of Presentation: 花王(株)霞ヶ浦研修所
  • Year and Date: 2011-10-15
• [Presentation] 人工セルロソームの構築と機能解析 (2011)
  • Author(s): 吉田和生、粟冠真紀子、木村哲哉、粟冠和郎
  • Organizer: 日本農芸化学会2011年度大会
  • Place of Presentation: 京都女子大学(京都市)
  • Year and Date: 2011-03-26
• [Presentation] Clostridium josui Cel9Aの酵素特性解析と多酵素複合体への利用 (2010)
  • Author(s): 小林崇也、粟冠真紀子、木村哲哉、粟冠和郎
  • Organizer: 酵素工学研究会第64回研究会
  • Place of Presentation: 東京大学山上会館(東京都)
  • Year and Date: 2010-11-19
• [Presentation] キメラ骨格タンパクを利用した人工セルロソームの構築 (2010)
  • Author(s): 米澤優希、粟冠真紀子、木村哲哉、粟冠和郎
  • Organizer: 酵素工学研究会第64回研究会
  • Place of Presentation: 東京大学山上会館(東京都)
  • Year and Date: 2010-11-19
• [Presentation] キメラ骨格タンパク質とミニセルロソームの構築 (2009)
  • Author(s): 小林崇也、粟冠真紀子、木村哲哉、粟冠和郎
  • Organizer: 第61回日本生物工学会大会
  • Place of Presentation: 名古屋大学
  • Year and Date: 2009-09-24
• [Book] 人工セルロソームの構築と酵母での発現、バイオマス分解研究の最前線 (2012)
  • Author(s): 粟冠和郎、幸田勝典
  • Publisher: シーエムシー出版
• [Book] バイオマス分解研究の最前線(人工セルロソームの構築と酵母での発現) (2012)
  • Author(s): 粟冠和郎、幸田勝典
  • Publisher: CMC出版