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Studies on structure and function of bacterial yellow affinity substance capable of enhancing enzymatic degradation of cellulose and its gene

Research Project

Project/Area Number 21380197
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Boundary agriculture
Research InstitutionMie University

Principal Investigator

SAKKA Kazuo  三重大学, 大学院・生物資源学研究科, 教授 (20154031)

Co-Investigator(Kenkyū-buntansha) SAKKA Makiko  三重大学, 大学院・生物資源学研究科 (00422882)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2009: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
KeywordsClostridium thermocellum / 黄色色素 / セルラーゼ / セルロソーム / セルロース分解
Research Abstract

Yellow affinity substance(YAS) extracted from cells of Clostridium thermocellum strain ATCC 27405 using acetone as the extracting solvent was adsorbed to microcrystalline cellulose(Funacel) and its digestibility was examined using some cellulase preparations. YAS-adsorbed Funacel was about 3-folds more efficiently degraded by cellulosome prepared from the culture supernatant of C. thermocellum ATCC 27405 than non-treated Funacel was. In addition, YAS-adsorbed Funacel was 2.5-folds more susceptible to mini-cellulosome constructed from three cellulases and a chimeric scaffolding protein than non-treated Funacel. This is the first study showing that YAS has an ability to enhance susceptibility of cellulose to cellulolytic enzymes.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (11 results)

All 2012 2011 2010 2009

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (5 results) Book (2 results)

  • [Journal Article] Analysis of cohesin-dockerin interactions using mutant dockerin proteins2011

    • Author(s)
      Kazutaka Sakka, Yuka Sugihara, Sadanari Jindou, Makiko Sakka, Minoru Inagaki, Kazuo Sakka and Tetsuya Kimura
    • Journal Title

      FEMS Microbiol Lett

      Volume: vol.314 Pages: 75-80

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of cohesin-dockerin interactions using mutant dockerin proteins2011

    • Author(s)
      K.Sakka, 他
    • Journal Title

      FEMS Microbiol., Lett.

      Volume: 314 Pages: 75-80

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Unusual binding properties of the dockerin module of Clostridium thermocellum endoglucanase CelJ(Cel9D-Cel44A)2009

    • Author(s)
      Kazutaka Sakka, Yuko Kishino, Yuka Sugihara, Sadanari Jindou, Makiko Sakka, Minoru Inagaki, Tetsuya Kimura and Kazuo Sakka
    • Journal Title

      FEMS Microbiol Lett

      Volume: vol.300 Pages: 249-255

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Unusual binding properties of the dockerin module of Clostridium thermocellum endoglucanase CelJ (Cel9D-Cel44A)2009

    • Author(s)
      K.Sakka, 他
    • Journal Title

      FEMS Microbiol., Lett. 300

      Pages: 249-255

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 人工ミニセルロソームにおけるファミリー3のCBMの機能解析2011

    • Author(s)
      吉田和生、粟冠真紀子、木村哲哉、粟冠和郎
    • Organizer
      セルラーゼ研究会
    • Place of Presentation
      花王(株)霞ヶ浦研修所
    • Year and Date
      2011-10-15
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 人工セルロソームの構築と機能解析2011

    • Author(s)
      吉田和生、粟冠真紀子、木村哲哉、粟冠和郎
    • Organizer
      日本農芸化学会2011年度大会
    • Place of Presentation
      京都女子大学(京都市)
    • Year and Date
      2011-03-26
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Clostridium josui Cel9Aの酵素特性解析と多酵素複合体への利用2010

    • Author(s)
      小林崇也、粟冠真紀子、木村哲哉、粟冠和郎
    • Organizer
      酵素工学研究会第64回研究会
    • Place of Presentation
      東京大学山上会館(東京都)
    • Year and Date
      2010-11-19
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] キメラ骨格タンパクを利用した人工セルロソームの構築2010

    • Author(s)
      米澤優希、粟冠真紀子、木村哲哉、粟冠和郎
    • Organizer
      酵素工学研究会第64回研究会
    • Place of Presentation
      東京大学山上会館(東京都)
    • Year and Date
      2010-11-19
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] キメラ骨格タンパク質とミニセルロソームの構築2009

    • Author(s)
      小林崇也、粟冠真紀子、木村哲哉、粟冠和郎
    • Organizer
      第61回日本生物工学会大会
    • Place of Presentation
      名古屋大学
    • Year and Date
      2009-09-24
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Book] 人工セルロソームの構築と酵母での発現、バイオマス分解研究の最前線2012

    • Author(s)
      粟冠和郎、幸田勝典
    • Publisher
      シーエムシー出版
    • Related Report
      2011 Final Research Report
  • [Book] バイオマス分解研究の最前線(人工セルロソームの構築と酵母での発現)2012

    • Author(s)
      粟冠和郎、幸田勝典
    • Publisher
      CMC出版
    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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