| Budget Amount *help |
¥18,980,000 (Direct Cost: ¥14,600,000、Indirect Cost: ¥4,380,000)
Fiscal Year 2011: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2010: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2009: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Research Abstract |
In order to improve the nitrogenase-based photobiological hydrogen production by cyanobacteria, we aimed at creating cyanobacteria mutants that preferentially express V or Fe-only type nitrogenase in place of Mo type, by genetic engineering. We have knocked out the Mo type nitrogenase gene of the cyanobacteria that have either Mo type and V type, or solely Mo type. We have prepared plasmids that harbor V or Fe-only type genes. In order to decrease the Mo possibly eluted from culture glass bottles into the culture medium, we developed hydrogen-gas-barrier plastic bags.
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