Molecular mechanisms of import to nuclei, mitochondria, and chloroplast, and UVB resistance in rice.
| Project/Area Number |
21380205
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
HIDEMA Jun 東北大学, 大学院・生命科学研究科, 准教授 (20250855)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAZAWA Yutaka 東北大学, 大学院・生命科学研究科, 助教 (00342858)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2009: ¥9,100,000 (Direct Cost: ¥7,000,000、Indirect Cost: ¥2,100,000)
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| Keywords | CPD光回復酵素 / オルガネラ移行シグナル / UVB抵抗性 / 植物 / 葉緑体 / ミトコンドリア / 光回復酵素 / シグナルペプチド / DNA修復 / ピリミジン二量体 / ミトコンドリアDNA修復 / タンパク質輸送 / イネ / 紫外線 |
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| Research Abstract |
Cyclobutane pyrimidine dimer (CPD)photolyase is major pathway for repairing CPD in plant, and CPD photolyase is a crucial factor for determining UVB sensitivity in plants. We found that CPD photolyase, which is encoded by a single-copy gene in the nuclear genome, is expressed and targeted not only to nuclei but also to mitochondria and chloroplasts. To clarify the molecular mechanisms of import to each organelle in rice, the subcellular localization analyses were performed. We found that the amino acids residues from 391 to 401 and from 487 to 489 are functional targeting signal for transport into mitochondria and nuclei, respectively. The results indicate that rice may have evolved a CPD photolyase that functions in all three organelles that contain DNA to protect cells from the harmful effects of UVB radiation.
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Report
(4 results)
Research Products
(54 results)
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[Journal Article] Eukaryotic Class II CPD photolyase structure reveals a basis for improved UV-tolerance in plants2012
Author(s)
Hitomi K, Arvai AS, Yamamoto J, Hitomi C, Teranishi M, Hirouchi T, Yamamoto K, Iwai S, Tainer JA, Hidema J, Getzoff ED
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Journal Title
J Biol Chem
Volume: 287
Pages: 12060-9
Related Report
Peer Reviewed
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