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Diversity and regulation of ascorbate biosynthesis in photosynthetic organisms

Research Project

Project/Area Number 21380207
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Applied molecular and cellular biology
Research InstitutionShimane University

Principal Investigator

ISHIKAWA Takahiro  島根大学, 生物資源科学部, 教授 (60285385)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥19,370,000 (Direct Cost: ¥14,900,000、Indirect Cost: ¥4,470,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2009: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Keywords光合成生物 / アスコルビン酸生合成 / 光調節 / バイオテクノロジー / 遺伝子 / アスコルビン酸 / 代謝調節機構
Research Abstract

Ascorbate biosynthesis pathway and its regulation mechanism have been studied in photosynthesis organisms. A moss, Physcomitrella patens, possesses both D-mannose/L-galactose pathway and D-galacturonate pathway, and an identification of aldonolactonase reveals that the enzyme plays a role in regulation of ascorbate level in the moss. It has also revealed that the alternative D-galacturonate pathway contributes to ascorbate accumulation in ripened tomato fruits. In Arabidopsis leaves, VTC2, one of the components of D-mannose/L-galactose pathway, plays a central role in light regulation of ascorbate via transcriptional and post-translational control.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (46 results)

All 2012 2011 2010 2009

All Journal Article (11 results) (of which Peer Reviewed: 11 results) Presentation (33 results) Book (2 results)

  • [Journal Article] Analysis of two L-galactono-1, 4-lactone responsive genes with complementary expression during the development of Arabidopsis thaliana2012

    • Author(s)
      Gao, Y., Badejo, A. A., Sawa, Y., Ishikawa, T.
    • Journal Title

      Plant Cell Physiol.

      Volume: Vol.53 Issue: 3 Pages: 592-601

    • DOI

      10.1093/pcp/pcs014

    • NAID

      10030309635

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Translocation and the alternative D-galacturonate pathway contribute to increasing ascorbate level in ripening tomato fruits together with the D-mannose/L-galactose pathway2012

    • Author(s)
      Badejo, A.A., Wada, K., et al
    • Journal Title

      Journal of Experimental Botany

      Volume: 63 Issue: 1 Pages: 229-239

    • DOI

      10.1093/jxb/err275

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 光合成生物におけるアスコルビン酸生合成研究の新展開2011

    • Author(s)
      石川孝博
    • Journal Title

      生化学

      Volume: 83巻 Pages: 838-841

    • NAID

      10029737825

    • URL

      http://wwwsoc.nii.ac.jp/jbiochem/magazine/83-09-05.pdf

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression analysis of the VTC2 and VTC5 genes encoding GDP-L-galactose phosphorylase, an enzyme involved in ascorbate biosynthesis, in Arabidopsis thaliana2011

    • Author(s)
      Gao, Y., Badejo, A. A., Shibata, H., Sawa, Y., Maruta, T., Shigeoka, S., Page, M., Smirnoff, N., Ishikawa, T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: Vol.75 Pages: 1783-1788

    • NAID

      10029757408

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of aspartyl protease and C3HC4-type RING zinc finger genes are responsive to ascorbic acid in Arabidopsis thaliana2011

    • Author(s)
      Gao, Y., Nishikawa, H., Badejo, A. A., Shibata, H., Sawa, Y., Nakagawa, T., Maruta, T., Shigeoka, S., Smirnoff, N., Ishikawa, T.
    • Journal Title

      J. Exp. Bot.

      Volume: Vol.62 Issue: 10 Pages: 3647-3657

    • DOI

      10.1093/jxb/err068

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Arabidopsis NADPH oxidases, Atrboh D and Atrboh F, are essential for jasmonic acid-induced expression of genes regulated by MYC2 transcription factor2011

    • Author(s)
      Maruta, T., Inoue, T., Tamoi, M., Yabuta, Y., Yoshimura, K., Ishikawa, T., Shigeoka, S.
    • Journal Title

      Plant Sci.

      Volume: Vol.180 Issue: 4 Pages: 655-660

    • DOI

      10.1016/j.plantsci.2011.01.014

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression Analysis of the VTC2 and VTC5 Genes Encoding GDP-L-Galactose Phosphorylase, an Enzyme Involved in Ascorbate Biosynthesis, in Arabidopsis thaliana2011

    • Author(s)
      Gao, Y., Badejo, A.A., et al
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 75 Issue: 9 Pages: 1783-1788

    • DOI

      10.1271/bbb.110320

    • NAID

      10029757408

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Contribution of Arabidopsis Homologs of L-Gulono-1,4-lactone Oxidase to the Biosynthesis of Ascorbic Acid2010

    • Author(s)
      Maruta, T., Ichikawa, Y., Mieda, T., Takeda, T., Tamoi, M., Yabuta, Y., Ishikawa, T., Shigeoka, S.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Issue: 7 Pages: 1494-1497

    • DOI

      10.1271/bbb.100157

    • NAID

      10027557628

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Euglena gracilis ascorbate peroxidase forms an intramolecular dimeric structure : its unique molecular characterization2010

    • Author(s)
      Ishikawa, T., Tajima, N., Nishikawa, H., Gao, Y., Rapolu, M., Shibata, H., Sawa, Y. and Shigeoka S.
    • Journal Title

      Biochem. J.

      Volume: Vol.426 Issue: 2 Pages: 125-134

    • DOI

      10.1042/bj20091406

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Contribution of Arabidopsis homologs of L-gulono-1,4-lactone oxidase to the biosynthesis of ascorbic acid.2010

    • Author(s)
      Maruta, T., Ichikawa, Y., et.al.
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 74 Pages: 1494-1497

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Euglena gracilis ascorbate peroxidase forms an intramolecular dimeric structure : its unique molecular characterization.2010

    • Author(s)
      Ishikawa, T., Tajima, N., et.al.
    • Journal Title

      Biochemical Journal

      Volume: 426 Pages: 125-134

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] トマト果実におけるアスコルビン酸蓄積機構の解明2012

    • Author(s)
      Adebanjo A. Badejo, 和田慶子, 丸田隆典, 澤嘉弘, 重岡成, 石川孝博
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都市・京都産業大学
    • Year and Date
      2012-03-16
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] シロイヌナズナvtc2変異体におけるアスコルビン酸取込み能の評価2012

    • Author(s)
      山本遥, 孝田翔, 丸田隆典, 澤嘉弘, 石川孝博
    • Organizer
      日本農芸化学会中四国支部第32回講演会
    • Place of Presentation
      鳥取市・鳥取大学
    • Year and Date
      2012-01-21
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] 蘇類ヒメツリガネゴケにおけるアルドノラクトナーゼの機能解析2012

    • Author(s)
      西川仁、丸田隆典、澤嘉弘、重岡成、石川孝博
    • Organizer
      第53回日本植物生理学会年会
    • Place of Presentation
      京都市・京都産業大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナGDP-L-ガラクトースホスホリラーゼ遺伝子の発現解析2012

    • Author(s)
      増澤拓也、小口能里枝、丸田隆典、澤嘉弘、重岡成、石川孝博
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都市・京都女子大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナにおける新奇アスコルビン酸欠乏変異体の機能解析2012

    • Author(s)
      原井健司、丸田隆典、澤嘉弘、重岡成、石川孝博
    • Organizer
      日本農芸化学会2012年度大会
    • Place of Presentation
      京都市・京都女子大学
    • Related Report
      2011 Annual Research Report
  • [Presentation] 蘚類ヒメツリガネゴケのアスコルビン酸生合成にかかわるアルドノラクトナーゼの機能解析2011

    • Author(s)
      西川仁, 丸田隆典, 澤嘉弘, 重岡成, 石川孝博
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of two DUF 642 family genes with complementary expression during the development of Arabidopsis thaliana2011

    • Author(s)
      高用順, 澤嘉弘, 石川孝博
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Year and Date
      2011-12-13
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Unfolding the mechanism of vitamin C accumulation in ripening Micro-Tom fruits2011

    • Author(s)
      Adebanjo A. Badejo, Takanori Maruta, Yoshihiro Sawa, Shigeru Shigeoka, Takahiro Ishikawa
    • Organizer
      8th Solanaceae and 2nd Cucurbitaceae Genome Joint Conference
    • Place of Presentation
      神戸市・コンベンションセンター
    • Year and Date
      2011-11-29
    • Related Report
      2011 Final Research Report
  • [Presentation] 青色光によるEuglenaアスコルビン酸生合成調節機構の検討2011

    • Author(s)
      松原まどか, 西川仁, 丸田隆典, 澤嘉弘, 重岡成, 石川孝博
    • Organizer
      ユーグレナ研究会第27回研究会
    • Place of Presentation
      春日井市・中部大学
    • Year and Date
      2011-11-12
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物ビタミンC生合成に関わるVTCC2/5遺伝子の機構解析2011

    • Author(s)
      高用順, 増澤拓也, 澤嘉弘, 柴田均, 丸田隆典, 重岡成, 石川孝博
    • Organizer
      日本ビタミン学会第63回大会
    • Place of Presentation
      広島市・安田女子大学
    • Year and Date
      2011-06-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナビタミンC欠乏変異体vtc3の機能解析2011

    • Author(s)
      原井健司、柴田均、澤嘉弘、丸田隆典、重岡成、石川孝博
    • Organizer
      日本ビタミン学会第63回大会
    • Place of Presentation
      広島市・安田女子大学
    • Year and Date
      2011-06-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Vitamin C Biosynthesis and Translocation in Tomato (Lycopersicum esculentum) Fruits2011

    • Author(s)
      A.A.Badejo, K.Wada, H.Shibata, Y.Sawa, T.Maruta, S.Shigeoka, T,Ishikawa
    • Organizer
      日本ビタミン学会第63回大会
    • Place of Presentation
      広島市・安田女子大学
    • Year and Date
      2011-06-05
    • Related Report
      2011 Annual Research Report
  • [Presentation] Vitamin C Biosynthesis and Translocation in Tomato(Lycopersicum esculentum) Fruits.2011

    • Author(s)
      Adebanjo A. Badejo, Keiko Wada, Hitoshi Shibata, Yoshihiro Sawa, Takanori Maruta, Shigeru Shigeoka, Takahiro Ishikawa
    • Organizer
      日本ビタミン学会第63回大会
    • Place of Presentation
      広島市・安田女子大
    • Year and Date
      2011-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] シロイヌナズナビタミンC欠乏変異体vtc3の機能解析2011

    • Author(s)
      原井健司, 柴田均, 澤嘉弘, 丸田隆典, 重岡成, 石川孝博
    • Organizer
      日本ビタミン学会第63回大会
    • Place of Presentation
      広島市・安田女子大
    • Year and Date
      2011-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 植物ビタミンC生合成に関わるVTC2/5遺伝子の機構解析2011

    • Author(s)
      高用順, 増澤拓也, 澤嘉弘, 柴田均, 丸田隆典, 重岡成, 石川孝博
    • Organizer
      日本ビタミン学会第63回大会
    • Place of Presentation
      広島市・安田女子大
    • Year and Date
      2011-06-04
    • Related Report
      2011 Final Research Report
  • [Presentation] 光合成生物におけるビタミンC研究の新展開2011

    • Author(s)
      石川孝博
    • Organizer
      日本農芸化学会中四国支部第4回農芸化学の未来開拓セミナー
    • Place of Presentation
      岡山市・岡山大学(招待講演)
    • Year and Date
      2011-05-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] シロイヌナズナアスコルビン酸欠乏変異体vtc3の機能解析2011

    • Author(s)
      原井健司, 石川孝博, 他5名
    • Organizer
      日本農芸化学会
    • Place of Presentation
      京都市
    • Year and Date
      2011-03-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] トマト果実におけるアスコルビン酸プールサイズ調節機構の解明2011

    • Author(s)
      高用順, 増澤拓也, Badejo A. Adebanjo, 柴田均, 澤嘉弘, Smirnoff Nicholas, 丸田隆典, 重岡成, 石川孝博
    • Organizer
      第52回日本植物生理学会年会
    • Place of Presentation
      仙台市(要旨公表成立)
    • Year and Date
      2011-03-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Regulation of ascorbate pool size in tomato fruits.2011

    • Author(s)
      Adebanjo A. Badejo, Keiko Wada, Takanori Maruta, Yoshihiro Sawa, Shigeru Shigeoka, Takahiro Ishikawa
    • Organizer
      The 10th International Conference on Reactive Oxygen and Nitrogen Species in Plants
    • Place of Presentation
      Budapest, Hungary
    • Related Report
      2011 Final Research Report
  • [Presentation] Regulation of Ascorbate Pool Size in Tomato Fruits2011

    • Author(s)
      AA.Badejo, K.Wada, T.Maruta, Y,Sawa, S.Shigeoka, T.Ishikawa
    • Organizer
      10th International Conference on Reactive Oxygen and Nitrogen Species in Plants
    • Place of Presentation
      Budapest, Hungary
    • Related Report
      2011 Annual Research Report
  • [Presentation] 蘇類ヒメツリガネゴケのアスコルビン酸生合成にかかわるアルドノラクトナーゼの機能解析2011

    • Author(s)
      西川仁、丸田隆典、澤嘉弘、重岡成、石川孝博
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Related Report
      2011 Annual Research Report
  • [Presentation] アスコルビン酸生合成に関わるシロイヌナズナVTC2/5遺伝子の発現解析2010

    • Author(s)
      増澤拓也, 高用順, 澤嘉弘, 柴田均, 丸田隆典, 重岡成, 石川孝博
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      神戸市・コンベンションセンター
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] トマト果実成熟過程におけるアスコルビン酸生合成関連酵素遺伝子の発現解析2010

    • Author(s)
      和田慶子, Badejo A. Adebanjo, 澤嘉弘, 柴田均, 丸田隆典, 重岡成, 石川孝博
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      (神戸市)神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2011 Final Research Report
  • [Presentation] アスコルビン酸生合成に関わるシロイヌナズナVTC2/5遺伝子の発現解析2010

    • Author(s)
      増澤拓也, 石川孝博, 他4名
    • Organizer
      日本分子生物学会
    • Place of Presentation
      神戸市
    • Year and Date
      2010-12-07
    • Related Report
      2010 Annual Research Report
  • [Presentation] アスコルビン酸生合成に関わるヒメツリガネゴケ由来アルドノラクトナーゼ遺伝子の解析2010

    • Author(s)
      石川孝博, 西川仁
    • Organizer
      第132回ビタミンC研究委員会
    • Place of Presentation
      東京・お茶の水大
    • Year and Date
      2010-07-24
    • Related Report
      2011 Final Research Report
  • [Presentation] 蘚類ヒメツリガネゴケのアスコルビン酸生合成に関わるアルドノラクトナーゼの機能解析2010

    • Author(s)
      西川仁, 原井健司, 澤嘉弘, 丸田隆典, 重岡成, 石川孝博
    • Organizer
      第62回日本ビタミン学会
    • Place of Presentation
      (盛岡市)岩手県公会堂
    • Year and Date
      2010-06-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Ascorbate Responsive Genes in Arabidopsis thaliana.2010

    • Author(s)
      Yongshun Gao, Yoshihiro Sawa, Tsuyoshi Nakagawa, Nicholas Smirnoff, Shigeru Shigeoka, Takahiro Ishikawa
    • Organizer
      21th International Conference on Arabidopsis Research
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      2010-06-07
    • Related Report
      2011 Final Research Report
  • [Presentation] ヌクレオシド二リン酸キナーゼはアスコルビン酸生合成の光調節に関与するか?2010

    • Author(s)
      有田未希、丸田隆典、澤嘉弘、柴田均、重岡成、石川孝博
    • Organizer
      2010年度日本農芸化学会年会
    • Place of Presentation
      東京駒場・東京大学
    • Year and Date
      2010-03-28
    • Related Report
      2011 Final Research Report
  • [Presentation] シロイヌナズナにおけるアスコルビン酸応答遺伝子の探索2010

    • Author(s)
      高用順、澤嘉弘、柴田均、中川強、Nicholas Smirnoff、重岡成、石川孝博
    • Organizer
      第51回日本植物生理学会年会
    • Place of Presentation
      熊本市・熊本大学
    • Year and Date
      2010-03-19
    • Related Report
      2011 Final Research Report
  • [Presentation] ユーグレナL-ガラクトノ-1, 4-ラクトン脱水素酵素の機能解析2009

    • Author(s)
      西川仁、松原まどか、澤嘉弘、柴田均、薮田行哲、丸田隆典、重岡成、石川孝博
    • Organizer
      第32回日本分子生物学会年会
    • Place of Presentation
      横浜市・パシフィコ横浜
    • Year and Date
      2009-12-10
    • Related Report
      2011 Final Research Report
  • [Presentation] ユーグレナL-ガラクトノ-1,4-ラクトン脱水素酵素の機能解析2009

    • Author(s)
      西川仁, 石川孝博, 他6名
    • Organizer
      日本分子生物学会
    • Place of Presentation
      横浜市
    • Year and Date
      2009-12-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蘚類ヒメツリガネゴケのアスコルビン酸生合成経路の解析2009

    • Author(s)
      西川仁, 石川孝博, 他5名
    • Organizer
      日本ビタミン学会
    • Place of Presentation
      京都府亀岡市
    • Year and Date
      2009-05-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] 蘚類ヒメツリガネゴケのアスコルビン酸生合成経路の解析2009

    • Author(s)
      西川仁, 澤嘉弘, 柴田均, 薮田行哲, 丸田隆典, 重岡成, 石川孝博
    • Organizer
      第61回日本ビタミン学会
    • Place of Presentation
      京都府亀岡市・京都学園大学
    • Year and Date
      2009-05-30
    • Related Report
      2011 Final Research Report
  • [Book] II 9.ビタミンC(9. 2. 2生合成経路と酸化還元系)ビタミン総合辞典2010

    • Author(s)
      石川孝博、重岡成
    • Publisher
      朝倉書店
    • Related Report
      2011 Final Research Report
  • [Book] ビタミン総合辞典2010

    • Author(s)
      石川孝博, 重岡成
    • Total Pages
      624
    • Publisher
      朝倉書店
    • Related Report
      2010 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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