Development of a new therapeutic treatment for metabolic diseases based on the solution of the relationship between bile acids and homeostasis of lipids and sugars
| Project/Area Number |
21390006
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Tohoku University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIMADA Miki 東北大学, 病院, 准教授 (10196488)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2009: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
|
|---|
| Keywords | 分析化学 / メタボロミクス / 抱合型胆汁酸 / プロテオミクス / 翻訳後修飾 / 質量分析 / 胆汁酸結合タンパク質 / 3次元培養 / 2次元分離 / 胆汁酸 / シグナル伝達 / デオキシコール酸 / ケノデオキシコール酸 / 2次元ゲル電気泳動 / 結合タンパク質 |
|---|
| Research Abstract |
We analyzed proteome in cultured cells after bile acid stimulation, and found quantitative variation for posttranslational modifications of α-enolase and peroxiredoxin-1. By cleavable affinity gel extraction, we found intracellular carrier proteins and some enzymes for bile acid metabolism besides peroxiredoxin-1 as bile acid binding proteins in hepatocytes. We developed a method for one 18O-atom labeling at C-terminal of tryptic digested peptides, which allowed application to quantitative proteomics. The method for comprehensive analysis of bile acid conjugates developed in this study was useful for finding specific metabolites in some diseases.
|
Report
(4 results)
Research Products
(197 results)
-
-
-
-
-
-
-
-
[Journal Article] Biliary bile acids in birds of the Cotingidae family : Taurine-conjugated (24R, 25R)-3α, 7α, 24-trihydroxy-5β-cholestan-27-oic acid and two epimers (25R and 25S)of 3α, 7α-dihydroxy-5β-cholestan-27-oic acid2011
Author(s)
Lee R. Hagey, Takashi Iida, Shoujiro Ogawa, Yuuki Adachi, Mizuho Une, Kumiko Mushiake, Masamitsu Maekawa, Miki Shimada, Nariyasu Mano, and Alan F. Hofmann
-
Journal Title
Steroids
Volume: 76
Pages: 1126-1135
Related Report
Peer Reviewed
-
[Journal Article] Chemical synthesis of (22E)-3α, 6α, 7α, 12α-tetrahydroxy-5β-chol-22-en-24-oic acid and its N-acylamidated conjugates with glycine or taurine : Precursors of the [22, 23-3H] labelled tracers2011
Author(s)
Shoujiro Ogawa, Yuuki Adachi, Genta Kakiyama, Miki Shimada, Nariyasu Mano, Junichi Goto, and Takashi Iida
-
Journal Title
Chem. Pharm. Bull
Volume: 58
Pages: 1103-1106
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] Chemical synthesis of 3-and 21-monosulfates and their double-conjugates of tetrahydrocorticosteroids in the 5α-and 5β-series2010
Author(s)
Rika Okihara, Kuniko Mitamura, Maki Hasegawa, Megumi Mori, Akina Muto, Genta Kakiyama, Shoujiro Ogawa, Takashi Iida, Miki Shimada, Nariyasu Mano, and Shigeo Ikegawa : Potential corticoid metabolites
-
Journal Title
Chem. Pharm. Bull
Volume: 58
Pages: 344-353
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] A new, major C27 biliary bile acid in the red-winged tinamou (Rhynchotus rufescens):(25R)-1β, 3α, 7α-trihydroxy-5β-cholestan-27-oic acid2009
Author(s)
Lee R. Hagey, Genta Kakiyama, Akina Muto, Takashi Iida, Kumiko Mushiake, Takaaki Goto, Nariyasu Mano, Junichi Goto, Cleida A. Oliveria, and Alan F. Hofmann
-
Journal Title
J. Lipid Res
Volume: 50
Pages: 651-657
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
[Journal Article] DCK is frequently inactivated in acquired gemcitabine-resistant human cancer cells
Author(s)
Yuriko Saiki, Yuki Yoshino, Hiroko Fujimura, Tatsuya Manabe, Yuki Kudo, Miki Shimada, Nariyasu Mano, Tomohiro Nakano, Yoonha Lee, Shinjiro Shimizu, Shinya Oba, Sho Fujiwara, Hideyuki Shimizu, Na Chen, Zhaleh K Nezhad, Guo Jin, Shinichi Fukushige, Makoto Sunamura, Masaharu Ishida, Fuyuhiko Motoi, Shinichi Egawa, Michiaki Unno, and Akira Horii
-
Journal Title
Biochem. Biophys. Res. Commun., in press
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Determination of multi-conjugated bile acids in the urine of patients with Niemann-Pick disease, type C by liquid chromatography/electrospray ionization tandem mass spectrometry2010
Author(s)
Masamitsu Maekawa, Yasushi Misawa, Ayako Sotoura, Osamu Sakamoto, Toshihiro Ohura, Daiki Abukawa, Takehiko Inoue, Kousaku Ohno, Akina Muto, Hajime Takei, Hiroshi Nittono, Shoujiro Ogawa, Genta Kakiyama, Takashi Iida, Alan F. Hofmann, Junichi Goto, Miki Shimada, and Nariyasu Mano
Organizer
XXI International Bile Acid Meeting ; Bile Acids as Metabolic Integrators and Therapeutics
Place of Presentation
Freiburg, Germany(Poster)
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
