Visualization and analysis of receptor oligomerization in living cells by using novel fluorescence labeling method
| Project/Area Number |
21390007
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Physical pharmacy
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YANO Yoshiaki 京都大学, 大学院・薬学研究科, 助教 (60402799)
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2010: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2009: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
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| Keywords | 蛍光イメージング / GPCR / オリゴマー化 / βアドレナリン受容体 / 蛍光励起エネルギー移動 / コンフォメーション変化 / β2アドレナリン受容体 / グルタミン酸受容体 |
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| Research Abstract |
An accurate method to analyze oligomeric state of membrane proteins in living cells by using novel fluorescence labeling method(coiled-coil labeling method) has been established. The method enables analysis of association number of target proteins, which has been difficult by using conventional methods.
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Report
(4 results)
Research Products
(31 results)
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[Presentation] Oligomerization of membrane receptors2010
Author(s)
Yoshiaki Yano, Kaoru Omae, and Katsumi Matsuzaki
Organizer
FRET analysis using coiled-coil tag-probe labeling and spectral imaging Biophysical Society 54th annual meeting Aan Francisco
Place of Presentation
USA
Year and Date
2010-10-23
Related Report
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