Design and delivery of plasmid vector for spatiotemporal control of the expression of therapeutic protein and siRNA
| Project/Area Number |
21390009
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Physical pharmacy
|
|---|
| Research Institution | Kyoto University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
NISHIKAWA Makiya 京都大学, 大学院・薬学研究科, 准教授 (40273437)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2011: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2010: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
|
|---|
| Keywords | ドラッグデリバリー / 遺伝子治療 / RNA干渉 / プラスミドDNA / 融合タンパク質 / デリバリー |
|---|
| Research Abstract |
Interferon-γ(IFN-γ) was selected as a therapeutic protein and several IFN-γgene therapy systems were developed. Selection of proper promoters of IFN-γ-expressing vectors resulted in the regulation of the time profile of IFN-γtransgene expression. A fusion of IFN-γwith a protein that regulates the tissue distribution resulted in the spatiotemporal control of IFN-γafter in vivo gene transfer. This control led to an increase in the therapeutic effect of IFN-γ-based gene therapy on metastatic tumor with reduction in adverse effects.
|
Report
(4 results)
Research Products
(37 results)
