| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2011: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2010: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2009: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000)
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| Research Abstract |
We previously reported that the ubiquitin-proteasome system(UPS) plays a key extracellular role in fertilization, as an egg-coat lysin, which allows sperm to penetrate through the egg-coat in the ascidians H. roretziand C. intestinalis. However, the structural features and the sorting mechanism of the sperm UPS are not known. Here, we investigated the sperm-specific UPS using these ascidians. We found that a certain ubiquitin-conjugating enzyme(Ube2r) is specifically expressed in the testis of C. intestinalis and that the C-terminal 16 residues of theα6/PSMA1 subunit of the sperm 20S proteasome are specifically processed in H. roretzi. These findings enabled us to further investigate the possible involvement of the C-terminal processing of the proteasome in the sorting of the proteasome to an acrosome or outer surface of sperm head regions.
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