Assessment of in vivo mutagenicity and trans-generational effect of compounds contained in suspended particulate matter in urban air

• Project/Area Number: 21390038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental pharmacy
• Research Institution: National Institute for Environmental Studies
• Principal Investigator: AOKI Yasunobu 独立行政法人国立環境研究所, 環境リスク研究センター, 副センター長 (20159297)
• Co-Investigator(Kenkyū-buntansha): NOUMI Takehiko 国立医薬品食品衛生研究所, 変異遺伝部, 部長 (30150890) ; KAGEYAMA Shiho 郡山女子大学, 家政学部, 講師 (00514316) ; MATSUMOTO Michi 独立行政法人国立環境研究所, 環境リスク研究センター, 主任研究員 (60132867) ; NAKAJIMA Daisuke 独立行政法人国立環境研究所, 環境リスク研究センター, 主任研究員 (10281411)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000); Fiscal Year 2011: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2010: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2009: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
• Keywords: 環境系薬学 / 有害大気汚染物質 / 体内変異原性 / 肺 / 精巣 / 多環芳香族炭化水素 / In vivo mutation / gpt deltaマウス / 経気道曝露 / ディーゼルナノ粒子 / 点突然変異 / 環境質定量化・予測 / 人間生活環境 / 有害化学物質 / 遺伝子 / 癌

Research Abstract

We evaluated mutagenesis induced in the target organs, such as the lung, by exposure of total suspended particulate matter(TSP) in ambient air using a model transgenic rodent for detecting in mutations, named gpt delta mouse. Consequently, in vivo mutagenicity of TSP extract obtained in Tsukuba City was estimated as 0.76 x 10^<-5>/mg. Compounds causing in vivo mutagenisis in Tar have not been identified, but several observations suggest that any potent mutagens other than polycyclic aromatic hydrocarbons exist in ambient air.

Research Products

• [Journal Article] Evaluation of the in vivo Mutagenicity of Nickel Subsulfide in the Lung of F344 gpt delta Transgenic Rats Exposed by Intratracheal Instillation: A Collaborative Study for the gpt delta Transgenic Rat Mutation Assay (2012)
  • Author(s): Kamigaito T, Noguchi T, Narumi K, Takashima R, Hamada S, Sanada H, Hasuko M, Hayashi H, Masumura K, Nohmi T
  • Journal Title: Genes and Environment Volume: 34 Issue: 1 Pages: 34-44
  • DOI: 10.3123/jemsge.34.34
  • NAID: 130004481194
  • ISSN: 1880-7046, 1880-7062
  • Peer Reviewed
• [Journal Article] Evaluation of <i>In Vivo</i> Mutagenicity by 2,4-Diaminotoluene and 2,6-Diaminotoluene in Liver of F344 <i>gpt</i> delta Transgenic Rat Dosed for 28 Days: A Collaborative Study of the <i>gpt</i> delta Transgenic Rat Mutation Assay (2012)
  • Author(s): Sui H, Ohta R, Shiragiku T, Akahori A, Suzuki K, Nakajima M, Hayashi H, Masumura K, Nohmi T
  • Journal Title: Genes and Environment Volume: 34 Issue: 1 Pages: 25-33
  • DOI: 10.3123/jemsge.34.25
  • NAID: 130004481193
  • ISSN: 1880-7046, 1880-7062
  • Peer Reviewed
• [Journal Article] Estimation of daily inhalation rate in preschool children using a tri-axial accelerometer : a pilot study (2011)
  • Author(s): Kawahara J, Tanaka S, Tanaka C, Aoki Y, Yonemoto J
  • Journal Title: Sci Total Environ Volume: 409 Pages: 3037-3077
  • Peer Reviewed
• [Journal Article] Evaluation of the in vivo mutagenicity of nickel subsulfide in the lung of F344 gpt delta transgenic rats exposed by intratracheal instillation : A collaborative study for the gpt delta transgenic rat mutation assay (2011)
  • Author(s): Kamigaito T, Noguchi T, Narumi K, Takashima R, Hamada S, Sanada H, Hasuko M, Hayashi H, Masumura K, Nohmi T
  • Journal Title: Genes En viron Volume: 34 Pages: 34-44
  • Peer Reviewed
• [Journal Article] Evaluation of in vivo mutagenicity by 2, 4-diaminotoluene and 2, 6-diaminotoluene in liver of F344 gpt delta transgenic rat dosed for 28 days : a collaborative study of the gpt delta transgenic rat mutation assay (2011)
  • Author(s): Sui H, Ohta R, Shiragiku T, Akahori A, Suzuki K, Nakajima M, Hayashi H, Masumura K, Nohmi T
  • Journal Title: Genes Environ Volume: 34 Pages: 25-33
  • Peer Reviewed
• [Journal Article] Estimation of the respiratory ventilation rate of preschool children in daily life using accelerometers (2011)
  • Author(s): Kawahara J, Tanaka S, Tanaka C, Aoki Y, Yonemoto J
  • Journal Title: J. Air Waste Manag. Assoc Volume: 61 Pages: 46-54
  • Peer Reviewed
• [Journal Article] Application of chemical reaction mechanistic domains to an ecotoxicity QSAR model, the KAshinhou Tool for Ecotoxicity (KATE) (2011)
  • Author(s): Furuhama A, Hasunuma K, Aoki Y, Yoshioka Y, Shiraishi H
  • Journal Title: SAR QSAR Environ Res Volume: 22 Pages: 505-523
  • Peer Reviewed
• [Journal Article] Estimation of the respiratory ventilation rate of preschool children in daily life using accelerometers (2011)
  • Author(s): Kawahara J., Tanaka S., Tanaka C., Aoki Y., Yonemoto J.
  • Journal Title: J.Air Waste Manag.Assoc. Volume: 61(1) Pages: 46-54
  • Peer Reviewed
• [Journal Article] Potency of air pollutants at DNA adduct formation and assessment by in vivo mutagenesis (2010)
  • Author(s): Aoki Y, Hashimoto AH, Amanuma K, Matsumoto M
  • Journal Title: DNA adduct formation, detection and mutagenesis.(ed.Alvarez E.and Cunha R.)(Nova Science Publishers) Pages: 143-153
  • Peer Reviewed
• [Presentation] Comparative study on the utilities of animal experimental data for risk assessment of inhalation exposure of chemicals (2012)
  • Author(s): Aoki Y, Matsumoto Y, Matsumoto M
  • Organizer: 51st Annual Meeting, Society of Toxicology
  • Place of Presentation: Mosconw Convention Center(San Francisco)
  • Year and Date: 2012-03-12
• [Presentation] Comparative study on the utilities of animal experimental data for risk assessment of inhalation exposure of chemicals (2012)
  • Author(s): Aoki Y, Matsumoto Y, Matsumoto M
  • Organizer: 51st Annual Meeting, Society of Toxicology
  • Place of Presentation: Mosconw Convention Center (San Francisco)
  • Year and Date: 2012-03-12
• [Presentation] Health risk assessemnt of air pollutants : Air pollutant genotoxicity and its enhancement on suppression of phase II drug-metabolizing enzymes (2011)
  • Author(s): Aoki Y
  • Organizer: International Symposium on Genotoxic and Carcinogenicity Thresholds
  • Place of Presentation: 一橋記念講堂(東京都)
  • Year and Date: 2011-11-23
• [Presentation] Health risk assessemnt of air pollutants : Air pollutant genotoxicity and its enhancement on suppression of phase II drug-metabolizing enzymes (2011)
  • Author(s): Aoki Y
  • Organizer: International Symposium on Genotoxic and Carcinogenic Thresholds
  • Place of Presentation: 一橋記念講堂(東京都)
  • Year and Date: 2011-11-23
• [Presentation] 大気汚染物質によるgpt deltaマウス肺中の突然変異とヒト肺がん組織中p53遺伝子の突然変異の比較 (2011)
  • Author(s): 青木康展,松本理,能美健彦
  • Organizer: 日本環境変異原学会第40回大会
  • Place of Presentation: 一橋記念講堂(東京都)
  • Year and Date: 2011-11-21
• [Presentation] 遺伝子発現プロファイルと分子ネットワークによる都市大気成分の毒性寄与予測手法の開発 (2011)
  • Author(s): 佐藤陽美,中島大介,影山志保,後藤純雄,松下秀鶴,渡辺徹志,青木康展
  • Organizer: 日本薬学会第131年会
  • Place of Presentation: グランシップ(静岡県)
  • Year and Date: 2011-03-30
• [Presentation] 遺伝子発現プロファイルと分子ネットワークによる都市大気成分の毒性寄与予測手法の開発 (2011)
  • Author(s): 佐藤陽美, 中島大介, 影山志保, 後藤純雄, 松下秀鶴, 渡辺徹志, 青木康展
  • Organizer: 日本薬学会 第131年会
  • Place of Presentation: グランシップ(静岡県)
  • Year and Date: 2011-03-30
• [Presentation] In vivo bioassays for assessing the total environmental risk (2011)
  • Author(s): Aoki Y
  • Organizer: Environmental Health 2011
  • Place of Presentation: Bahia Othon Palace Hotel(Salvador, Brasil)
  • Year and Date: 2011-02-09
• [Presentation] In vivo bioassays for assessing the total environmental risk (2011)
  • Author(s): Aoki Y.
  • Organizer: Environmental Health 2011
  • Place of Presentation: Bahia Othon Palace Hotel (Salvador, Brasil)
  • Year and Date: 2011-02-09
• [Presentation] In vivo mutagenicity of ambient air in the lungs of gpt delta transgenic mice ; A case study in Tsukuba city, Japan (2010)
  • Author(s): Sato H, Nakajima D, Kageyama S, Sakashita Y, Yanagisawa R, Masumura K, Nohmi T, Aoki Y
  • Organizer: 2^<nd> Asian Conf. Environ. Mutagens
  • Place of Presentation: Dusit Thani Hotel(Pattaya, Thailand)
  • Year and Date: 2010-12-16
• [Presentation] In vivo mutagenicity of ambient air in the lungs of gpt delta transgenicmice ; A case study in Tsukuba city, Japan. (2010)
  • Author(s): Sato H., Nakajima D., Kageyama S., Sakashita Y., Yanagisawa R., Masumura K., Nohmi T., Aoki Y.
  • Organizer: 2nd Asian Conf.Environ.Mutagens
  • Place of Presentation: Dusit Thani Hotel (Pattaya, Thailand)
  • Year and Date: 2010-12-16
• [Presentation] 日本人幼児の1日肺換気量 (2010)
  • Author(s): 河原純子,田中千晶,田中茂穂,青木康展,米元純三
  • Organizer: 日本リスク研究学会第23回年次大会
  • Place of Presentation: 明治大学駿河台キャンパス(東京都)
  • Year and Date: 2010-11-28
• [Presentation] 日本人幼児の1日肺換気量 (2010)
  • Author(s): 河原純子, 田中千晶, 田中茂穂, 青木康展, 米元純三
  • Organizer: 日本リスク研究学会 第23回年次大会
  • Place of Presentation: 明治大学駿河台キャンパス(東京都)
  • Year and Date: 2010-11-28
• [Presentation] 大気中の発がん物質のリスク評価-疫学研究データと動物実験データに基づく評価値の比較- (2010)
  • Author(s): 松本理,青木康展
  • Organizer: 日本環境変異原学会第39回大会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2010-11-17
• [Presentation] 大気中の粒子状物質抽出物がgpt deltaマウス肺中に示す変異原性; 2009年つくば市内, 1989年バンコク市内の試料について (2010)
  • Author(s): 青木康展,佐藤陽美,中島大介,影山志保,阪下由香利,柳澤利枝,後藤純雄,松下秀鶴,増村健一,能美健彦
  • Organizer: 日本環境変異原学会第39回大会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2010-11-17
• [Presentation] ディーゼルナノ粒子長期曝露によりgpt deltaマウス肺・肝臓に誘導される突然変異 (2010)
  • Author(s): 佐藤陽美,阪下由香利,増村健一,古山昭子,平野靖史郎,能美健彦,青木康展
  • Organizer: 日本環境変異原学会第39回大会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2010-11-17
• [Presentation] 大気中の発がん物質のリスク評価-疫学研究データと動物実験データに基づく評価値の比較- (2010)
  • Author(s): 松本理, 青木康展
  • Organizer: 日本環境変異原学会 第39回大会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2010-11-17
• [Presentation] 大気中の粒子状物質抽出物がgpt deltaマウス肺中に示す変異原性;2009年つくば市内,1989年バンコク市内の試料について (2010)
  • Author(s): 青木康展, 佐藤陽美, 中島大介, 影山志保, 阪下由香利, 柳澤利枝, 後藤純雄, 松下秀鶴, 増村健一
  • Organizer: 日本環境変異原学会 第39回大会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2010-11-16
• [Presentation] ディーゼルナノ粒子長期曝露によりgpt deltaマウス肺・肝臓に誘導される突然変異 (2010)
  • Author(s): 佐藤陽美, 阪下由香利, 増村健一, 古山昭子, 平野靖史郎, 能美健彦, 青木康展
  • Organizer: 日本環境変異原学会 第38回大会
  • Place of Presentation: つくば国際会議場(茨城県)
  • Year and Date: 2010-11-16
• [Presentation] 国内5地点で採取した都市大気浮遊粉じんの変異原性 (2010)
  • Author(s): 影山志保,稲葉洋平,佐藤陽美,松本理,青木康展,嵐谷奎一,矢島博文,中島大介,後藤純雄
  • Organizer: 第16回バイオアッセイ研究会・日本環境毒性学会合同研究発表会
  • Place of Presentation: つくば研究支援センター(茨城県)
  • Year and Date: 2010-09-02
• [Presentation] Assessment of in vivo mutagenesis induced by environmental chemicals using transgenic animals for detecting mutagens (2009)
  • Author(s): Aoki Y
  • Organizer: 2nd International Conference on Environmental Health Science
  • Place of Presentation: Korea Institute of Science and Technology(Seoul, Korea)
  • Year and Date: 2009-10-30
• [Presentation] Assessment of in vivo mutagenesis induced by environmental chemicals using transgenic animals for detecting mutagens (2009)
  • Author(s): Aoki Y.
  • Organizer: 2nd International Conference on Environmental Health Science
  • Place of Presentation: Seoul
  • Year and Date: 2009-10-30
• [Presentation] In vivo mutagenesis induced by the air pollutants in the testis of gpt delta transgenic mice (2009)
  • Author(s): Aoki Y, Hashimoto AH, Amanuma K, Matsumoto M, Masumura K, Nohmi T
  • Organizer: 21st Cent. Adv. Mol. Toxicol. Environ. Chem. Pathog. Dis
  • Place of Presentation: 東京大学医学部鉄門講堂(東京都)
  • Year and Date: 2009-10-26
• [Presentation] In vivo mutagenesis induced by the air pollutants in the test is of gpt delta transgenic mice (2009)
  • Author(s): Aoki Y, Hashimoto AH, Amanuma K, Matsumoto M, Masumura K, Nohmi T
  • Organizer: 21st Cent.Adv.Mol.Toxicol.Environ.Chem.Pathog.Dis.
  • Place of Presentation: Tokyo
  • Year and Date: 2009-10-26
• [Presentation] Enhanced in vivo mutations in the lung of phase II enzyme-suppressed mice (2009)
  • Author(s): Aoki Y
  • Organizer: X International Conference on Environmental Mutagens
  • Place of Presentation: Congress Center(Firenze, Italy)
  • Year and Date: 2009-08-22
• [Presentation] Enhanced in vivo mutations in the lung of phase II enzyme-suppressed mice (2009)
  • Author(s): Aoki Y
  • Organizer: X International Conference on Environmental Mutagens
  • Place of Presentation: Firenze
  • Year and Date: 2009-08-22
• [Book] Potency of air pollutants at DNA adduct formation and assessment by in vivo mutagenesis. In DNA adduct formation, detection and mutagenesis (2010)
  • Author(s): Aoki Y, Hashimoto AH, Amanuma K, Matsumoto M
  • Publisher: Nova Science Publishers Inc