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Elucidation of functions of genes involved in cell polarity in vivo

Research Project

Project/Area Number 21390050
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General anatomy (including Histology/Embryology)
Research InstitutionOsaka University

Principal Investigator

HARADA Akihiro  大阪大学, 医学系研究科, 教授 (40251441)

Co-Investigator(Kenkyū-buntansha) SATO Ken  群馬大学, 生体調節研究所, 教授 (30311343)
SATO Takashi  群馬大学, 生体調節研究所, 准教授 (70344934)
Project Period (FY) 2009 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2011: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2009: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Keywords細胞極性 / ノックアウトマウス / 極性輸送 / 線虫 / Rab8 / 線毛
Research Abstract

To elucidate the molecular mechanism of cell polarity, we knocked out genes involved in polarized transport. In addition, we tried to find out novel genes involved in cell polarity using the worm, C.elegans. As we found various phenotypes in our knockout mice, we are preparing manuscripts for the analyses of knockout mice. We finished screening of C.elegans in search of novel genes. We are now making knockout mice for some of the novel genes now.

Report

(4 results)
  • 2012 Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (47 results)

All 2012 2011 2010 2009 Other

All Journal Article (20 results) (of which Peer Reviewed: 20 results) Presentation (24 results) (of which Invited: 1 results) Remarks (3 results)

  • [Journal Article] Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector.2012

    • Author(s)
      Hashimoto Y
    • Journal Title

      FASEB Journal

      Volume: 26 Issue: 11 Pages: 4662-4674

    • DOI

      10.1096/fj.12-207530

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Delivery of endosomes to lysosomes via microautophagy in the visceral endoderm of mouse embryos2012

    • Author(s)
      Kawamura, Wada et al.
    • Journal Title

      Nature Communications

      Volume: 3 Issue: 1 Pages: 1071-1071

    • DOI

      10.1038/ncomms2069

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cdc42 and Rab8a are critical for intestinal stem cell division, survival, and differentiation in mice.2012

    • Author(s)
      Sakamori R
    • Journal Title

      J Clin Invest

      Volume: 122 Issue: 3 Pages: 1052-1065

    • DOI

      10.1172/jci60282

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deficiency of proton-sensing ovarian cancer G protein-coupled receptor 1 attenuates glucose- stimulated insulin secretion2012

    • Author(s)
      Nakakura T
    • Journal Title

      Endocrinology

      Volume: 153 Issue: 9 Pages: 4171

    • DOI

      10.1210/en.2012-1164

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Critical roles of type III phosphatidylinositol phosphate kinase in murine embryonic visceral endoderm and adult intestine2012

    • Author(s)
      Takasuga , Wada et al.
    • Journal Title

      PNAS 110

      Volume: 1726-1731 Issue: 5 Pages: 1726-31

    • DOI

      10.1073/pnas.1213212110

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Caenorhabditis elegans SNAP-29 isrequired for organellar integrity of the endomembrane system and general exocytosis in intestinal epithelial cells.2011

    • Author(s)
      Sato M, Saegusa K, Sato K, Hara T, Harada A, Sato K.
    • Journal Title

      Molecular Biology of the Cell

      Volume: 22 Issue: 14 Pages: 2579-2587

    • DOI

      10.1091/mbc.e11-04-0279

    • NAID

      120004898416

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] The role of VAMP7/TI-VAMP in cell polarity and lysosomal exocytosis in vivo.2011

    • Author(s)
      Sato M, Yoshimura S, Hirai R, Goto A, Kunii M, Atik N, Sato T, Sato K, Harada R, Shimada J, Hatabu T, Yorifuji H, Harada A.
    • Journal Title

      Traffic

      Volume: 12 Issue: 10 Pages: 1383-1393

    • DOI

      10.1111/j.1600-0854.2011.01247.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanism of polarized transport.2010

    • Author(s)
      Harada A.
    • Journal Title

      Journal of Biochemistry

      Volume: 147 Issue: 5 Pages: 619-624

    • DOI

      10.1093/jb/mvq027

    • NAID

      10027875494

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of liver steatosis and lipid droplet formation in ATF6alpha-knockout mice burdened with pharmacological endoplasmic reticulum stress.2010

    • Author(s)
      Yamamoto K, Takahara K, Oyadomari S, Okada T, Sato T, Harada A, Mori K.
    • Journal Title

      Molecular Biology of the Cell

      Volume: 21 Issue: 17 Pages: 2975-2986

    • DOI

      10.1091/mbc.e09-02-0133

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Induction of liver steatosis and lipid droplet formation in ATF6alpha-knockout mice burdened with pharmacological endoplasmic reticulum stress.2010

    • Author(s)
      Yamamoto K, Takahara K, Oyadomari S, Okada T, Sato T, Harada A, Mori K.
    • Journal Title

      Mol Biol Cell.

      Volume: 21 Pages: 2975-2986

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular mechanism of polarized transport.2010

    • Author(s)
      Harada A
    • Journal Title

      J.Biochem.

      Volume: 147 Pages: 619-624

    • NAID

      10027875494

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of proton-sensing TDAG8 in extracellular acidification-induced inhibition of proinflammatory cytokine production in peritoneal macrophages.2009

    • Author(s)
      Mogi C, Tobo M, Tomura H, Murata N, He XD, Sato K, Kimura T, Ishizuka T, Sasaki T, Sato T, Kihara Y, Ishii S, Harada A, Okajima F.
    • Journal Title

      Journal of Immunology

      Volume: 182 Issue: 5 Pages: 3243-3251

    • DOI

      10.4049/jimmunol.0803466

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] SAP-1 is a microvillus-specific protein tyrosine phosphatase that modulates intestinal tumorigenesis.2009

    • Author(s)
      Sadakata H, Okazawa H, Sato T, Supriatna Y, Ohnishi H, Kusakari S, Murata Y, Ito T, Nishiyama U, Minegishi T, Harada A, Matozaki T.
    • Journal Title

      Genes to Cells

      Volume: 14 Issue: 3 Pages: 295-308

    • DOI

      10.1111/j.1365-2443.2008.01270.x

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Investigation of the role of oligopeptide transporter PEPT1 and sodium/glucose cotransporter SGLT1 in intestinal absorption of their substrates using small GTP-binding protein Rab8-null mice2009

    • Author(s)
      Kato Y, Sugiura T, Nakadera Y, Sugiura M, Kubo Y, Sato T, Harada A, Tsuji A.
    • Journal Title

      Drug Metabolism and Disposition

      Volume: 37 Issue: 3 Pages: 602

    • DOI

      10.1124/dmd.108.023689

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] p31 deficiency influences endoplasmic reticulum tubular morphology and cell survival.2009

    • Author(s)
      Uemura T, Sato T, Aoki T, Yamamoto A, Okada T, Hirai R, Harada R, Mori K, Tagaya M, Harada A.
    • Journal Title

      Molecular and Cellular Biology

      Volume: 29 Issue: 7 Pages: 1869-1881

    • DOI

      10.1128/mcb.01089-08

    • NAID

      120004898418

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Generation of cortactin floxed mice and cellular analysis of motility in fibroblasts2009

    • Author(s)
      Tanaka, S., Kunii, M., Harada, A., Okabe, S
    • Journal Title

      Genesis

      Volume: 47 Issue: 9 Pages: 638-646

    • DOI

      10.1002/dvg.20544

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel role for hGas7b in microtubular maintenance: possible implication in tau-associated pathology in Alzheimer disease.2009

    • Author(s)
      Akiyama H, Gotoh A, Shin RW, Koga T, Ohashi T, Sakamoto W, Harada A, Arai H, Sawa A, Uchida C, Uchida T.
    • Journal Title

      Journal of Biological Chemistry

      Volume: 284 Issue: 47 Pages: 32695-32699

    • DOI

      10.1074/jbc.m109.035998

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] p31 deficiency influences endoplasmic reticulum tubular morphology and cell survival.2009

    • Author(s)
      Takefumi Uemura
    • Journal Title

      Molecular and Cellular Biology 29

      Pages: 1869-1881

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Generation of cortactin floxed mice and cellular analysis of motility in fibroblasts.2009

    • Author(s)
      Shinji Tanaka
    • Journal Title

      Genesis 47

      Pages: 638-646

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel role for hGas7b in microtubular maintenance : possible implication in tau-associated pathology in Alzheimer disease2009

    • Author(s)
      Hirotada Akiyama
    • Journal Title

      The Journal of Biological Chemistry 284

      Pages: 32695-32699

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 上皮細胞の極性輸送を司る分子の欠損マウスの解析2012

    • Author(s)
      原田彰宏,國井政孝,吉村信一郎
    • Organizer
      日本解剖学会
    • Place of Presentation
      山梨大学甲府キャンパス
    • Year and Date
      2012-03-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 脳の形態形成における小胞輸送関連分子 SNAP23 の機能の解明2012

    • Author(s)
      國井政孝、原田彰宏
    • Organizer
      日本解剖学会
    • Place of Presentation
      山梨大学甲府キャンパス
    • Year and Date
      2012-03-27
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内極性輸送に関わる遺伝子の欠損マウスの神経細胞の極性形成について2012

    • Author(s)
      原田彰宏
    • Organizer
      日本神経科学会大会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Annual Research Report
  • [Presentation] Roles of membrane trafficking in cell polarity and secretion in model animals2012

    • Author(s)
      原田彰宏
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2011 Annual Research Report
    • Invited
  • [Presentation] Uncovering genes required for neuronal morphology by morphology-based gene trap screening with a revertible retrovirus vector2012

    • Author(s)
      原田彰宏
    • Organizer
      日本分子生物学会
    • Place of Presentation
      福岡
    • Related Report
      2011 Annual Research Report
  • [Presentation] The role of VAMP7/TI-VAMP in cell polarity and lysosomal exocytosis in vivo (ポスター)2011

    • Author(s)
      原田彰宏
    • Organizer
      日本 分子生物学会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-12-16
    • Related Report
      2012 Final Research Report
  • [Presentation] SNARE 分子のノックアウトマウスにおける神経系の表現型の解析(ポスター)2011

    • Author(s)
      原田彰宏
    • Organizer
      日本神経科学大会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2011-09-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内極性輸送を司る分子の組織・個体における機能の分子細胞生物学的解析2011

    • Author(s)
      原田彰宏
    • Organizer
      日本顕微鏡学会
    • Place of Presentation
      福岡国際会議場
    • Year and Date
      2011-05-16
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内極性輸送を司る分子を欠損したマウスの作製とその細胞極性の解析(ポスター)2011

    • Author(s)
      原田彰宏
    • Organizer
      日本生理学会大会・日本解剖学会合同大会
    • Place of Presentation
      パシフィコ横浜(震災のため誌上開催)
    • Year and Date
      2011-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内極性輸送を司る分子を欠損したマウスの作製とその細胞極性の解析2011

    • Author(s)
      原田彰宏
    • Organizer
      第88回日本生理学会大会 第116回日本解剖学会総会・全国学術集会合同大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2011-03-30
    • Related Report
      2010 Annual Research Report
  • [Presentation] 上皮細胞の極性と機能における、細胞内極性輸送を司る分子の役割とその欠損による病態2010

    • Author(s)
      原田彰宏
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートピアホテル
    • Year and Date
      2010-12-10
    • Related Report
      2012 Final Research Report
  • [Presentation] 上皮細胞の極性と機能における、細胞内極性輸送を司る分子の役割とその欠損による病態2010

    • Author(s)
      原田彰宏
    • Organizer
      BMB2010(第33回日本分子生物学会年会、第83回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸ポートピアホテル(兵庫県神戸市)(招待講演)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] 細胞内極性輸送を司る分子の欠損マウスの神経の解析(ポスター)2010

    • Author(s)
      原田彰宏
    • Organizer
      Neuro2010
    • Place of Presentation
      神戸国際展示場
    • Year and Date
      2010-09-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞内極性輸送を司る分子の欠損マウスの神経の解析2010

    • Author(s)
      原田彰宏
    • Organizer
      Neuro2010(第33回日本神経科学大会・第53回日本神経科学会大会・第20回日本神経回路学会大会)
    • Place of Presentation
      神戸国際展示場(兵庫県神戸市)
    • Year and Date
      2010-09-02
    • Related Report
      2010 Annual Research Report
  • [Presentation] 選別輸送関連蛋白 PKD1,PKD2遺伝子欠損マウスの解析(ポスター)2010

    • Author(s)
      國井政孝、古本奈緒美、佐藤隆史、稲見圭子、原田彰宏
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      大阪国際会議場
    • Year and Date
      2010-05-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 選別輸送関連蛋白PKD1,PKD2遺伝子欠損マウスの解析2010

    • Author(s)
      國井政孝、古本奈緒美、佐藤隆史、稲見圭子、原田彰宏
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪国際会議場(大阪府大阪市)
    • Year and Date
      2010-05-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] 低分子量 GTPase Rab8 の生体における機能について2009

    • Author(s)
      原田彰宏
    • Organizer
      日本生化学会
    • Place of Presentation
      神戸国際会議場
    • Year and Date
      2009-10-23
    • Related Report
      2012 Final Research Report
  • [Presentation] シンポジウム「低分子量GTPaseから眺めるメンブレントラフィック研究の新展開」(招待演者)「低分子量GTPase Rab8の生体における機能について」2009

    • Author(s)
      原田彰宏
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-10-23
    • Related Report
      2009 Annual Research Report
  • [Presentation] マウスでできること-ノックアウトマウスの作り方と解析について(イブニングレクチャー)2009

    • Author(s)
      原田彰宏
    • Organizer
      日本細胞生物学会
    • Place of Presentation
      名古屋国際会議場
    • Year and Date
      2009-06-02
    • Related Report
      2012 Final Research Report
  • [Presentation] 極性輸送関連分子欠損マウスを用いた細胞極性形成、維持のメカニズムの解明(解剖学・生理学連携シンポジウム)2009

    • Author(s)
      原田彰宏、佐藤隆史
    • Organizer
      日本解剖学会
    • Place of Presentation
      岡山理科大学
    • Year and Date
      2009-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞極性関連分子 VAMP7 欠失マウスの解析2009

    • Author(s)
      佐藤真人、 佐藤隆史、植村武文、國井政孝、多鹿友喜、村上徹、依藤宏、原田彰宏
    • Organizer
      日本解剖学会
    • Place of Presentation
      岡山理科大学
    • Year and Date
      2009-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞極性の維持における Rab8a および Rab8b の役割2009

    • Author(s)
      佐藤隆史、佐藤健、佐藤美由紀、平井里香、原田玲子、原田彰宏
    • Organizer
      日本解剖学会
    • Place of Presentation
      岡山理科大学
    • Year and Date
      2009-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 細胞極性及び脂質輸送関連タンパクFAPP1, 2 のノックアウトマウスの解析2009

    • Author(s)
      植村武文、古本奈緒美、佐藤隆史、原田彰宏
    • Organizer
      日本解剖学会
    • Place of Presentation
      岡山理科大学
    • Year and Date
      2009-03-30
    • Related Report
      2012 Final Research Report
  • [Presentation] 選別輸送関連蛋白 PKD1,PKD2遺伝子欠損マウスの作製および解析(ポスター)2009

    • Author(s)
      國井政孝、古本奈緒美、佐藤隆史、稲見圭子、原田彰宏
    • Organizer
      日本解剖学会
    • Place of Presentation
      岩手県民会館
    • Year and Date
      2009-03-29
    • Related Report
      2012 Final Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/acb/index.html

    • Related Report
      2012 Final Research Report
  • [Remarks] 研究代表者の研究室のHP

    • URL

      http://www.med.osaka-u.ac.jp/pub/acb/index.html

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.med.osaka-u.ac.jp/pub/acb/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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