Development of scanning probe microscopy in biomedical fields.

• Project/Area Number: 21390051
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General anatomy (including Histology/Embryology)
• Research Institution: Niigata University
• Principal Investigator: USHIKI Tatsuo 新潟大学, 医歯学系, 教授 (40184999)
• Co-Investigator(Kenkyū-buntansha): HOSHI Osamu 新潟大学, 医歯学系, 准教授 (10303124) ; KOGA Daisuke 新潟大学, 医歯学系, 助教 (30467071) ; NAKAJIMA Masato 新潟大学, 医歯学系, 助教 (60588250)
• Co-Investigator(Renkei-kenkyūsha): IWATA Futoshi 静岡大学, 工学部, 教授 (30262794)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2009: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: 走査型プローブ顕微鏡 / 原子間力顕微鏡 / 走査型イオン伝導顕微鏡 / マニピュレーション / 細胞

Research Abstract

In this project, we studied applicability of the following three techniques of scanning probe microscopy(SPM) to biological fields : 1) Real-time imaging in liquid by atomic force microscopy(AFM) 2) Scanning ion-conductance microscopy(SICM) 3) Manipulation using AFM manipulators Our results showed that these techniques are useful in the field of microscopic anatomy and expected to become powerful tools for imaging and manipulation of cells and tissues.

Research Products

• [Journal Article] Scanning ionconductance microscopy for imagingbiological samples in liquid Acomparative study Scanning ionconductance microscopy for imagingbiological samples in liquid (2012)
  • Author(s): Ushiki T
  • Journal Title: Acomparative study with atomic forcemicroscopy and scanning electronmicroscopy
  • Peer Reviewed
• [Journal Article] Nanomanipulation of biological samples using a compactatomic force microscope under scanningelectron microscope observation. (2012)
  • Author(s): Iwata F
  • Journal Title: J Electron Microsc Volume: 60 Issue: 6 Pages: 359-366
  • DOI: 10.1093/jmicro/dfr070
  • Peer Reviewed
• [Journal Article] 走査型プローブ顕微鏡で染色体の構造を解明する (2012)
  • Author(s): 中島真人、牛木辰男
  • Journal Title: 遺伝 Volume: 66 Pages: 119-124
• [Journal Article] 電子顕微鏡におけるAFMのマニピュレータ利用 (2012)
  • Author(s): 岩田太、牛木辰男
  • Journal Title: O plus E Volume: 34 Pages: 229-234
• [Journal Article] Scanning ion conductance microscopy for imaging biological samples in liquid : A comparative study with atomic force microscopy and scanning electron microscopy (2012)
  • Author(s): Ushiki T, et al.
  • Journal Title: Micron Volume: (In press) Issue: 12 Pages: 1390-1398
  • DOI: 10.1016/j.micron.2012.01.012
  • Peer Reviewed
• [Journal Article] Replication bandingpatterns in human chromosomes detectedusing Replication bandingpatterns in human chromosomes detectedusing (2011)
  • Author(s): Hoshi O
  • Journal Title: Acta Histochem Cytochem Volume: 44 Pages: 233-237
  • Peer Reviewed
• [Journal Article] Replication banding patterns in human chromosomes detected using 5-ethynyl-2'-deoxyuridine incorporation (2011)
  • Author(s): Hoshi O, et al.
  • Journal Title: Acta Histochem Cytochem Volume: 44 Pages: 233-237
  • NAID: 130001089433
  • Peer Reviewed
• [Journal Article] Operation of Self-Sensitive Cantilever in Liquid for Multiprobe Manipulation (2010)
  • Author(s): Iwata, F
  • Journal Title: Jpn J Appl Phys Volume: 49
  • NAID: 210000069137
  • Peer Reviewed
• [Journal Article] リアルタイム3D走査電子顕微鏡の医学生物応用 (2010)
  • Author(s): 牛木辰男, 他
  • Journal Title: 顕微鏡 Volume: 45 Pages: 198-201
  • NAID: 130007788878
  • Peer Reviewed
• [Journal Article] 走査型電子顕微鏡と細胞・組織の3Dイメージング技術の進展 (2010)
  • Author(s): 牛木辰男
  • Journal Title: 新潟医師会報 Volume: 475 Pages: 1-8
• [Journal Article] Operation of Self-Sensitive Cantilever in Liquid for Multiprobe Manipulation. (2010)
  • Author(s): Iwata, F, et al.
  • Journal Title: Jpn J Appl Phys Volume: 49
  • NAID: 210000069137
  • Peer Reviewed
• [Presentation] 細胞分裂中期の分裂装置と走査型イオン伝導顕微鏡解析 (2012)
  • Author(s): 中島真人、牛木辰男
  • Organizer: 第117回日本解剖学会総会・全国学術集会
  • Place of Presentation: 山梨大学甲府キャンパス(山梨県)
  • Year and Date: 2012-03-27
• [Presentation] 走査型イオン伝導顕微鏡(SICM)の生物応用 (2011)
  • Author(s): 牛木辰男、中島真人
  • Organizer: 日本顕微鏡学会関西支部会特別講演会(招待講演)
  • Place of Presentation: ウィンクあいち(愛知県)
  • Year and Date: 2011-12-28
• [Presentation] 走査型イオン伝導顕微鏡(SICM)の生物応用 (2011)
  • Author(s): 牛木辰男、中島真人
  • Organizer: 日本顕微鏡学会関西支部会特別講演会
  • Place of Presentation: ウィンクあいち(愛知県)(招待講演)
  • Year and Date: 2011-12-28
• [Presentation] 形態学から見た染色体の高次構造-走査電子顕微鏡と走査プローブ顕微鏡によるアプローチからー (2011)
  • Author(s): 牛木辰男、他(招待講演)
  • Organizer: 染色体学会第62回年会(招待講演)
  • Place of Presentation: 神奈川大学(神奈川県)
  • Year and Date: 2011-11-12
• [Presentation] 形態学から見た染色体の高次構造-走査電子顕微鏡と走査プローブ顕微鏡によるアプローチから- (2011)
  • Author(s): 牛木辰男, 他
  • Organizer: 染色体学会第62回年会
  • Place of Presentation: 神奈川大学(神奈川県)(招待講演)
  • Year and Date: 2011-11-12
• [Presentation] SEM技術現代から未来へ (2011)
  • Author(s): 牛木辰男
  • Organizer: (医生物)日本顕微鏡学会第67回学術講演会(指定講演)
  • Place of Presentation: 福岡国際会議場(福岡市)
  • Year and Date: 2011-05-17
• [Presentation] SEM技術現代から未来へ(医生物) (2011)
  • Author(s): 牛木辰男
  • Organizer: 日本顕微鏡学会第67回学術講演会
  • Place of Presentation: 福岡国際会議場(福岡市)(指定講演)
  • Year and Date: 2011-05-17
• [Presentation] 動原体の構造機能解析のために分裂装置を単離する (2011)
  • Author(s): 中島真人、他
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] リアルタイム3D走査電子顕微鏡内での生物試料のマニピュレーション (2011)
  • Author(s): 牛木辰男、他
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: 横浜
  • Year and Date: 2011-03-28
• [Presentation] リアルタイム3D走査電子顕微鏡内での生物試料のマニピュレーション (2011)
  • Author(s): 牛木辰男, 他
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: 横浜(誌上開催)
  • Year and Date: 2011-03-28
• [Presentation] 動原体の構造機能解析のために分裂装置を単離する (2011)
  • Author(s): 中島真人, 他
  • Organizer: 第116回日本解剖学会全国学術集会
  • Place of Presentation: 横浜(誌上開催)
  • Year and Date: 2011-03-28
• [Presentation] Atomic force microscopy andits related techniques for biologicalstudies (2011)
  • Author(s): Ushiki T
  • Organizer: Russian Nanotox Week2011(招待講演)
  • Place of Presentation: Vladivostok, Russia
• [Presentation] Interactivenanomanipulator coupled with a highspeed imaging technique (2011)
  • Author(s): Iwata F
  • Organizer: International scanning probe microscopy conference 2011
  • Place of Presentation: TUM-IAS(Munchen, Germany)
• [Presentation] Atomic force microscopy of biological samples in a real-time stereo scanning electron microscope (2011)
  • Author(s): Ushiki T
  • Organizer: International scanning probe microscopyconference 2011
  • Place of Presentation: TUM-IAS(Munchen, Germany)
• [Presentation] Atomic force microscopy of biological samples in a real-time stereo scanning electron microscope (2011)
  • Author(s): Ushiki T, et al.
  • Organizer: International scanning probe microscopy conference 2011
  • Place of Presentation: TUM-IAS (Munchen, Germany)
• [Presentation] Interactive nanomanipulator coupled with a high speed imaging technique (2011)
  • Author(s): Iwata F, et al.
  • Organizer: International scanning probe microscopy conference 2011
  • Place of Presentation: TUM-IAS (Munchen, Germany)
• [Presentation] Atomic force microscopy and its related techniques for biological studies (2011)
  • Author(s): Ushiki T
  • Organizer: Russian Nanotox Week 2011
  • Place of Presentation: (Vladivostok, Russia)Far Eastern Federal University(招待講演)
• [Presentation] AFMナノマニピュレータを用いたバイオサンプルの顕微解剖 (2010)
  • Author(s): 浅井慶祐,河西晋佐,佐々木彰,牛木辰男,岩田太
  • Organizer: 2009年度精密工学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-13
• [Presentation] AFMナニマニピュレータを用いたバイオサンプルの顕微解剖 (2010)
  • Author(s): 浅井慶祐, 河西晋佐, 佐々木彰, 牛木辰男, 岩田太
  • Organizer: 2009年度精密工学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-13
• [Presentation] Collagen fibrilformation by human osteosarcoma cells observed by atomic force microscopy (2010)
  • Author(s): Hoshi O and Ushiki T
  • Organizer: Bristol nano science symposium 2010
  • Place of Presentation: Bristol, UK
• [Presentation] Multiprobe manipulation of biologicalsamples using compact atomic forcemicroscopies (2010)
  • Author(s): Iwata F and Ushiki T(招待講演)
  • Organizer: XXIInternationalSymposium on Morphological Sciences
  • Place of Presentation: Taormina, Italy
• [Presentation] リアルタイムステレオSEMによる生物試料のマニピュレーション (2010)
  • Author(s): 牛木辰男、他
  • Organizer: 日本顕微鏡学会第66回学術集会
  • Place of Presentation: 名古屋
• [Presentation] Collagen fibrilformation by human osteosarcoma cellsobserved by atomic force microscopy (2010)
  • Author(s): Hoshi O and Ushiki T
  • Organizer: The 12th International Scanning Probe Microscopy Conference
  • Place of Presentation: 札幌
• [Presentation] Compactmanipulation based on an atomic force microscope coupled with a haptic device for multi-probe manipulation of biological samples (2010)
  • Author(s): Iwata F and Ushiki T
  • Organizer: The 12th International Scanning Probe Microscopy Conference
  • Place of Presentation: 札幌
• [Presentation] 細胞と組織の構造機能解析ツールとしての原子間力顕微鏡 (2010)
  • Author(s): 牛木辰男
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] 培養細胞系を用いたコラーゲン線維形成過程の原子間力顕微鏡による構造解析 (2010)
  • Author(s): 星治、牛木辰男
  • Organizer: 第115回日本解剖学会全国学術集会
  • Place of Presentation: 盛岡
• [Presentation] Compact manipulation based on an atomic force microscope coupled with ahaptic device for multi-probe manipulation of biological samples. (2010)
  • Author(s): Iwata F, Ushiki T
  • Organizer: The 12th International Scanning Probe Microscopy Conference
  • Place of Presentation: 札幌
• [Presentation] Collagen fibril formation by human osteosarcoma cells observed by atomic force microscopy. (2010)
  • Author(s): Hoshi O, Ushiki T
  • Organizer: The 12th International Scanning Probe Microscopy Conference
  • Place of Presentation: 札幌
• [Presentation] Collagen fibril formation by human osteosarcoma cells observed by atomic force microscopy. (2010)
  • Author(s): Hoshi O, Ushiki T
  • Organizer: Bristol nano science symposium 2010
  • Place of Presentation: Bristol, UK
• [Presentation] Multiprobe manipulation of biological samples using compact atomic force microscopies (2010)
  • Author(s): Iwata F, Ushiki T
  • Organizer: XXI International Symposium on Morphological Sciences
  • Place of Presentation: Taormina, Italy(招待講演)
• [Presentation] Compact AFM for multi-probemanipulation of biological samples in liquid condition (2009)
  • Author(s): Mizuguchi Y, Ozawa K, Ushiki T and Iwata F
  • Organizer: The 17th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: Atagawa, Japan
  • Year and Date: 2009-12-10
• [Presentation] Compact AFN for multi-probe manipulation of biological samples in liquid condition. (2009)
  • Author(s): Y.Mizuguchi, K.Ozawa, T.Ushiki, F.Iwata
  • Organizer: The 17th International Colloquium on Scanning Probe Microscopy
  • Place of Presentation: Atagawa, Japan
  • Year and Date: 2009-12-10
• [Presentation] 走査型イオン伝導顕微鏡(SICM)のバイオ応用 (2009)
  • Author(s): 牛木辰男、米谷雄作、岩田太
  • Organizer: 日本顕微鏡学会SPM研究部会
  • Place of Presentation: 越後湯沢
  • Year and Date: 2009-11-28
• [Presentation] 走査型イオン伝導顕微鏡(SICM)のバイオ応用 (2009)
  • Author(s): 牛木辰男、米谷雄作、岩田太
  • Organizer: 日本顕微鏡学会 SPM研究部会
  • Place of Presentation: 越後湯沢
  • Year and Date: 2009-11-28
• [Presentation] SEM内での顕微解剖可能なAFMマニピュレータの開発 (2009)
  • Author(s): 耕秀之、河西晋佐、青山尚之、牛木辰男、岩田太
  • Organizer: 応用物理学会(M & BE分科会)有機バイオSPM研究会
  • Place of Presentation: 幕張
  • Year and Date: 2009-09-04
• [Presentation] SEM内での顕微解剖可能なAFMマニピュレータの開発 (2009)
  • Author(s): 耕秀之、河西晋佐、青山尚之、牛木辰男、岩田太
  • Organizer: 応用物理学会(M&BE分科会)有機バイオSPM研究会
  • Place of Presentation: 幕張
  • Year and Date: 2009-09-04
• [Presentation] 電子顕微鏡内で動作する力覚提示AFMマニピュレータの開発と顕微解剖への応用 (2009)
  • Author(s): 耕秀之、河西晋佐、青山尚之、牛木辰男、岩田太
  • Organizer: 2009年第70回応用物理学会学術講演会
  • Place of Presentation: 富山
• [Presentation] An atomic force microscope coupled with a haptic device for observation and manipulation of biological samples in a scanning electron microscope (2009)
  • Author(s): Ushiki T, Iwata F
  • Organizer: VII International scanningprobe microscopy conference
  • Place of Presentation: Madrid, Spain
• [Presentation] An atomic force microscope coupled with a haptic device for observation and manipulation of biological samples in a scanning electron microscope. (2009)
  • Author(s): T.Ushiki, F.Iwata
  • Organizer: VII International scanning probe microscopy conference
  • Place of Presentation: Madrid, Spain
• [Presentation] 電子顕微鏡内で動作する力覚提示AFMマニピュレータの開発と顕微解剖への応用 (2009)
  • Author(s): 耕秀之、河西晋佐、青山尚之、牛木辰男、岩田太
  • Organizer: 2009年度精密工学会春季大会
  • Place of Presentation: 神戸
• [Book] 新・走査電子顕微鏡 (2011)
  • Author(s): 日本顕微鏡学会関東支部編
  • Publisher: 共立出版
• [Remarks]
  • URL: http://www.med.niigata-u.ac.jp/an3/welcome.html