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An interrelation among regulatory mechanisms of cardiac Ca2+channels

Research Project

Project/Area Number 21390059
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKagoshima University

Principal Investigator

KAMEYAMA Masaki  鹿児島大学, 医歯学総合研究科, 教授 (60150059)

Co-Investigator(Kenkyū-buntansha) YAZAWA Kazuto  鹿児島大学, 大学院・医歯学総合研究科, 講師 (90212274)
MINOBE Etsuko  鹿児島大学, 大学院・医歯学総合研究科, 助教 (00448581)
Co-Investigator(Renkei-kenkyūsha) XU Jian-jun  鹿児島大学, 大学院・医歯学総合研究科, 助教 (10581689)
Research Collaborator KAMEYAMA Asako  鹿児島大学, 大学院・医歯学総合研究科, 客員研究員
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords生体膜 / チャネル / シグナル伝達 / リン酸化 / トランスポーター / 能動輸送
Research Abstract

We investigated regulation of L-type Ca2+channels by calmodulin(CaM) and phosphorylation. CaM bound to preIQ and IQ regions in the C-terminal of the channel. PKA phosphorylated Ser1574 and Ser1626 as well as Ser1927(guinea-pig). The facilitatory effect of CaM was enhanced by CaMKII-mediated phosphorylation of Thr1603, suggesting a cross-talk between the CaM's effect and channel phosphorylation.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (50 results)

All 2012 2011 2010 2009 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (31 results) Remarks (4 results)

  • [Journal Article] Cloning and expression of the two new variants of Nav1.5/SCN5A in rat brain2012

    • Author(s)
      Ren CT, Li DM, Ou SW, Wang YJ, Lin Y, Zong ZH, Kameyama M, Kameyama A
    • Journal Title

      Mol.Cell.Biochem.

      Volume: (In press) Issue: 1-2 Pages: 139-148

    • DOI

      10.1007/s11010-012-1253-7

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Calpastatin domain L is a partial agonist of the calmodulin-binding site for channel activation in Cav12011

    • Author(s)
      Minobe E, Asmara H, Saud ZA, and Kameyama M.
    • Journal Title

      J Biol Chem

      Volume: 286(45) Pages: 39013-39022

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calpastatin domain L is a partial agonist of the calmodulin-binding site for channel activation in Cav1.2 Ca^<2+> channels2011

    • Author(s)
      Minobe E, Asmara H, Saud ZA, Kameyama M
    • Journal Title

      J.Biol.Chem.

      Volume: 286 Issue: 45 Pages: 39013-39022

    • DOI

      10.1074/jbc.m111.242248

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interactions of calmodulin with the multiple binding sites of Cav1. 2 Ca^<2+> channels2010

    • Author(s)
      Asmara H, Minobe E, Saud ZA, Kameyama M.
    • Journal Title

      J Pharmacol Sci

      Volume: 112(4) Pages: 397-404

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calmodulin-and Ca^<2+>-dependent facilitation and inactivation of the CaV1. 2 Ca^<2+> channels in guinea-pig ventricular myocytes2010

    • Author(s)
      Han DY, Minobe E, Wang WY, Guo F, Xu JJ, Hao LY, Kameyama M.
    • Journal Title

      J Pharmacol Sci

      Volume: 112(3) Pages: 310-319

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Both N-and C-lobes of calmodulin are required for Ca^<2+>-dependent regulations of CaV1. 2 Ca^<2+> channels2010

    • Author(s)
      Guo F, Minobe E, Yazawa K, Asmara H, Bai XY, Han DY, Hao LY and Kameyama M.
    • Journal Title

      Biochem Biophys Res Comm

      Volume: 391(2) Pages: 1170-1176

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calmodulin- and Ca^<2+>-dependent facilitation and inactivation of the CaV1.2 Ca^<2+> channels in guinea-pig ventricular myocytes.2010

    • Author(s)
      Han DY
    • Journal Title

      J Pharmacol Sci

      Volume: 112 Pages: 310-319

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Interactions of calmodulin with the multiple binding sites of Cavl.2 Ca^<2+> channels.2010

    • Author(s)
      Asmara H
    • Journal Title

      J Pharmacol Sci

      Volume: 112 Pages: 397-404

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Both N- and C-lobes of calmodulin are required for Ca^<2+> -dependent regulations of CaV1.2 Ca^<2+> channels2010

    • Author(s)
      Guo F.
    • Journal Title

      Biochem Biophys Res Comm 391

      Pages: 1170-1176

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The distinct roles of calmodulin and calmodulin kinase II in the reversal of run-down of L-type Ca^<2+> channels in guinea-pig ventricular myocytes2009

    • Author(s)
      Hao LY, Wang WY, Minobe M, Han DY, Xu JJ, Kameyama A, Kameyama M.
    • Journal Title

      J. Pharmacol. Sci

      Volume: 111(4) Pages: 416-425

    • NAID

      10026154787

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] CaMKII phosphorylates a threonine residue in the C-terminal tail of Cav1. 2 Ca^<2+> channel and modulates the interaction of the channel with calmodulin2009

    • Author(s)
      Wang WY, Hao LY, Minobe E, Saud ZA, Han DY, Kameyama M.
    • Journal Title

      J Physiol Sci

      Volume: 59(4) Pages: 283-90

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] CaMKII phosphorylates a threonine residue in the C-terminal tail of Cav1.2 Ca^<2+> channel and modulates the interaction of the channel with calmodulin2009

    • Author(s)
      Wang WY.
    • Journal Title

      J Physiol Sci 59

      Pages: 283-290

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of four novel variants of Nav1.5/SCN5A cloned from the brain2009

    • Author(s)
      Wang J.
    • Journal Title

      Neurosci Res 64

      Pages: 339-347

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Bio-assessment of RISK in long-term manned space exploration -Cell death factors in space radiation and/or microgravity : A review-2009

    • Author(s)
      Majima HJ.
    • Journal Title

      Biol Sci Space 23

      Pages: 43-53

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The distinct roles of calmodulin and calmodulin kinase II in the reversal of run-down of L-type Ca^<2+> channels in guinea-pig ventricular myocytes2009

    • Author(s)
      Hao LY.
    • Journal Title

      J Pharmacol Sc. 111

      Pages: 416-425

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] モルモット心室筋細胞におけるL型Ca^<2+>チャネルの過酸化水素による活性調節機構2012

    • Author(s)
      楊磊、徐建軍、蓑部悦子、封瑞、亀山亜砂子、矢沢和人、亀山正樹
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Cav1. 2 Ca^<2+>チャネルC末端部には、複数の機能的Aキナーゼリン酸化部位がある2012

    • Author(s)
      蓑部悦子、前田佐知子、徐建軍、はお麗英、封瑞、矢沢和人、亀山亜砂子、亀山正樹
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本
    • Related Report
      2011 Final Research Report
  • [Presentation] Cav1. 2 Ca^<2+>チャネルの活性調節におけるカルパスタチンとカルモジュリンの競合作用2012

    • Author(s)
      蓑部悦子、Hadhimulya Asmara, Zahangir A. Saud, 亀山正樹
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本
    • Related Report
      2011 Final Research Report
  • [Presentation] Cav1.2 Ca^<2+>チャネルの活性調節におけるカルパスタチンとカルモジュリンの競合作用2012

    • Author(s)
      蓑部悦子
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本
    • Related Report
      2011 Annual Research Report
  • [Presentation] Cav1.2 Ca^<2+>チャネルC末端部には、複数の機能的Aキナーゼリン酸化部位がある2012

    • Author(s)
      蓑部悦子
    • Organizer
      第89回日本生理学会大会
    • Place of Presentation
      松本
    • Related Report
      2011 Annual Research Report
  • [Presentation] CaMKII-dependent and independent facilitation of L-type Ca^<2+> channels by hydrogen peroxide in guinea-pig ventricular myocytes2011

    • Author(s)
      Yang L, Xu J-J, Minobe E, Feng R, Kameyama A, Yazawa K, Kameyama M.
    • Organizer
      The 62th meeting of the Physiological Society of West Japan
    • Place of Presentation
      Saga
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Facilitation of L-type Ca^<2+> channels by hydrogen peroxide in guinea-pig ventricular myocytes2011

    • Author(s)
      Yang L, Xu J-J, Minobe E, Feng R, Kameyama A, Yazawa K, Kameyama M.
    • Organizer
      The 5th Biennial Meeting of Society for Free Radical Research(SFRR)-Asia and The 8th Conference of Asian Society for Mitochondrial Research and Medicine(ASMRM)
    • Place of Presentation
      Kagoshima
    • Related Report
      2011 Final Research Report
  • [Presentation] Effect of Ca^<2+> and calmodulin on activity of calmodulin-linked Cav1. 2 channel2011

    • Author(s)
      Minobe E, Mori MX, Kameyama M.
    • Organizer
      The 88th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Both PKA-mediated phosphorylation and calmodulin interaction are required for basal activity of cardiac L-type Ca^<2+> channel2011

    • Author(s)
      Xu J-J, Saud ZA, Liu Y, Yang L, Minobe E, Kameyama A, Yazawa K, Hao L-Y, Kameyama M.
    • Organizer
      The 88th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Facilitation of L-type Ca2+ channels by hydrogen peroxide in guinea-pig ventricular myocytes2011

    • Author(s)
      Yang L
    • Organizer
      The 5th Biennial Meeting of Society for Free Radical Research (SFRR)-Asia and The 8th Conference of Asian Society for Mitochondrial Research and Medicine (ASMRM)
    • Place of Presentation
      Kagoshima
    • Related Report
      2011 Annual Research Report
  • [Presentation] カルパスタチンのL領域は、Cav1.2 Ca2+チャネルのカルモジュリンによる活性化部位の部分アゴニストとして働く2011

    • Author(s)
      蓑部悦子
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎
    • Related Report
      2011 Annual Research Report
  • [Presentation] PKA-mediated phosphorylation modulates the interaction of calmod ulin with cardiac Cav1. 2 channels2010

    • Author(s)
      Xu J-J, Saud ZA, Liu Y, Yang L, Minobe E, Kameyama A, Yazawa K, Hao L-Y, Kameyama M.
    • Organizer
      The 9th Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles
    • Place of Presentation
      Kagoshima
    • Related Report
      2011 Final Research Report
  • [Presentation] Ca^<2+>-dependent modulation in calmodulin-linked Cav1. 2 channels2010

    • Author(s)
      Minobe E, Mori MX, Xu J-J, Kameyama M.
    • Organizer
      The 9th Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles
    • Place of Presentation
      Kagoshima
    • Related Report
      2011 Final Research Report
  • [Presentation] Roles of calmodulin and the C-terminal tail of Cav1. 2 channel in its activity2010

    • Author(s)
      Minobe E, Mori MX, Kameyama M.
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      盛岡
    • Related Report
      2011 Final Research Report
  • [Presentation] Roles of calmodulin and the C-terminal tail of Cav1.2 channel in its activity.2010

    • Author(s)
      Minobe E
    • Organizer
      第87回日本生理学会大会
    • Place of Presentation
      盛岡
    • Related Report
      2010 Annual Research Report
  • [Presentation] Ca^<2+>-dependent modulation in calmodulin-linked Cav1.2 channels.2010

    • Author(s)
      Minobe E
    • Organizer
      The 9th Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles.
    • Place of Presentation
      Kagoshima
    • Related Report
      2010 Annual Research Report
  • [Presentation] PKA-mediated phosphorylation modulates the interaction of calmodulin with cardiac Cav1.2 channels.2010

    • Author(s)
      Xu J-J
    • Organizer
      The 9th Japan-Korea Joint Symposium on Brain Sciences, and Cardiac and Smooth Muscles.
    • Place of Presentation
      Kagoshima
    • Related Report
      2010 Annual Research Report
  • [Presentation] カルモジュリンをリンクさせたCav1.2型Ca^<2+>チャネルのCa^<2+>依存性調節2010

    • Author(s)
      蓑部悦子
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎
    • Related Report
      2010 Annual Research Report
  • [Presentation] Multiple molecules of calmodulin bind to the Cav1. 2 Ca^<2+> channel2009

    • Author(s)
      Asmara H, Minobe E, Saud ZA, Kameyama M.
    • Organizer
      The 60th meeting of the Physiological Society of West Japan
    • Place of Presentation
      Fukuoka
    • Related Report
      2011 Final Research Report
  • [Presentation] Regulation of Cav1. 2 Ca^<2+> channels by Ca^<2+>, calmodulin and CaMKII2009

    • Author(s)
      Kameyama M, Hao L-Y, Minobe E, Xu J-J, Han D-Y, Nie H-G, Saud ZA, Wang W-Y, Asmara H, Yazawa K.
    • Organizer
      IUPS Satellite Symposium on Ion channels : function, structure and physiology
    • Place of Presentation
      Hiei/Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] Multiple binding sites of calmodulin in the C-terminal tail of Cav1. 2 Ca^<2+> channel2009

    • Author(s)
      Asmara H, Minobe E, Saud ZA, Wang W-Y, Kameyama M.
    • Organizer
      The 36th International Congress of Physiological Sciences(IUPS2009)
    • Place of Presentation
      Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] Facilitatory and inhibitory effects of calmodulin on activity of Cav1. 2 Ca^<2+> channel2009

    • Author(s)
      Han D-Y, Hao L-Y, Minobe E, Wang W-Y, Xu J-J, Kamey ama M.
    • Organizer
      The 36th International Congress of Physiological Sciences(IUPS2009)
    • Place of Presentation
      Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] Calpastatin domain L competes with calmodulin on IQ region of Cav1. 2 channel2009

    • Author(s)
      Minobe E, Saud ZA, Han D-Y, Asmara H, Wang W-Y, Hao L-Y, Kameyama M.
    • Organizer
      The 36th International Congress of Physiological Sciences(IUPS2009)
    • Place of Presentation
      Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] Ca^<2+> channels and cardiac functions2009

    • Author(s)
      Kameyama M, Hao L-Y, Minobe E, Xu J-J, Han D-Y, Nie H-G, Saud ZA, Wang W-Y, Asmara H.
    • Organizer
      The 36th International Congress of Physiological Sciences(IUPS2009)
    • Place of Presentation
      Kyoto
    • Related Report
      2011 Final Research Report
  • [Presentation] Ca^<2+> channels and cardiac functions. The 36th International2009

    • Author(s)
      亀山正樹
    • Organizer
      Congress of Physiological Sciences(IUPS2009)
    • Place of Presentation
      Kyoto
    • Related Report
      2009 Annual Research Report
  • [Presentation] Calpastatin domain L competes with calmodulin on IQ region of Cav1.2 channel2009

    • Author(s)
      Minobe E.
    • Organizer
      Congress of Physiological Sciences(IUPS2009)
    • Place of Presentation
      Kyoto
    • Related Report
      2009 Annual Research Report
  • [Presentation] Facilitatory and inhibitory effects of calmodulin on activity of Cav1.2 Ca^<2+> channel2009

    • Author(s)
      Han DY.
    • Organizer
      Congress of Physiological Sciences(IUPS2009)
    • Place of Presentation
      Kyoto
    • Related Report
      2009 Annual Research Report
  • [Presentation] Multiple binding sites of calmodulin in the C-terminal tail of Cav1.2 Ca^<2+> channel2009

    • Author(s)
      Asmara H.
    • Organizer
      Congress of Physiological Sciences(IUPS2009)
    • Place of Presentation
      Kyoto
    • Related Report
      2009 Annual Research Report
  • [Presentation] Regulation of Cav1.2 Ca^<2+> channels by Ca^<2+>, calmodulin and CaMKII2009

    • Author(s)
      亀山正樹
    • Organizer
      IUPS Satellite Symposium on Ion channels : function, structure and physiology
    • Place of Presentation
      Hiei/Kyoto
    • Related Report
      2009 Annual Research Report
  • [Presentation] Multiple molecules of calmodulin bind to the Cav1.2 Ca^<2+> channel2009

    • Author(s)
      Asmara H.
    • Organizer
      The 60th meeting of the Physiological Society of West Japan
    • Place of Presentation
      Fukuoka
    • Related Report
      2009 Annual Research Report
  • [Presentation] カルモジュリンの心筋Ca^<2+>チャネル(Cav1.2)に対する二重作用2009

    • Author(s)
      亀山正樹
    • Organizer
      生理学研究所研究会
    • Place of Presentation
      岡崎市(愛知県)
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.kufm.kagoshima-u.ac.jp/~physiol2/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.kufm.kagoshima-u.ac.jp/~physio12/

    • Related Report
      2010 Annual Research Report
  • [Remarks]

    • URL

      http://www.kufm.kagoshima-u.ac.jp/~physiol2/

    • Related Report
      2009 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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