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Regulation of aquaporin-4 by formation of protein complex and orthogonal array

Research Project

Project/Area Number 21390061
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field General physiology
Research InstitutionKeio University

Principal Investigator

YASUI Masato  慶應義塾大学, 医学部, 教授 (90246637)

Co-Investigator(Kenkyū-buntansha) NAKAO Kazuki  独立行政法人理化学研究所, 動物実験支援ユニット, ユニットリーダー (20217657)
Co-Investigator(Renkei-kenkyūsha) ABE Yoichiro  慶應義塾大学, 医学部, 助教 (10317331)
YUKUTAKE Yoshinori  慶應義塾大学, 医学部, 助教 (80449016)
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2011: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2009: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Keywordsアクアポリン / 蛋白複合体 / 構造機能相関 / 水分子 / 脳浮腫 / 非線形光学 / コンピューターシミュレーション
Research Abstract

To investigate physiological roles of square array formation of aquaporin-4 (AQP4) in vivo, we established two kinds of AQP4 knockout mice: M1 isoform-specific KO, which is expected to possess much larger arrays than wild type, and null mice. As a result of comprehensive phenotype analysis, we found that appropriate size of the square arrays is essential for the retinal response to light stimulation. In addition, we demonstrated that AQP4 plays an important role for immunological function in the central nervous system by making stub-wounded model using AQP4-null mice. Both types of knockout mice have been deposited at RIKEN BRC so as to be used freely by other researchers.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (54 results)

All 2013 2012 2011 2010 2009 Other

All Journal Article (24 results) (of which Peer Reviewed: 24 results) Presentation (26 results) Remarks (4 results)

  • [Journal Article] Establishment of Monoclonal antibodies against the extracellular domain that block binding of NMO-IgG to AQP42013

    • Author(s)
      Miyazaki K, Abe Y, Iwanari H, Suzuki Y, Kikuchi T, Ito T, Kato J, Kusano-Arai O, Takahashi T, Nishiyama S, Ikeshima-Kataoka H, Tsuji S, Arimitsu T, Kato Y, Sakihama T, Toyama Y, Fujihara K, Hamakubo T and Yasui M
    • Journal Title

      J. Neuroimmunol

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Immunological function of aquaporin-4 in stab-wounded mouse brain in concert with a pro-inflammatory cytokine inducer, osteopontin2013

    • Author(s)
      Ikeshima-Kataoka H, Abe Y, Abe T, and Yasui M
    • Journal Title

      Mol. Cell. Neurosci

      Volume: (in press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An astrocyte-specific enhancer of aquaporin-4 gene functions through a consensus sequence of POU transcription factors in concert with multiple upstream elements2012

    • Author(s)
      Abe Y, Ikeshima-Kataoka H, Goda W, Niikura T, and Yasui M
    • Journal Title

      J. Neurochem

      Volume: 120(6) Pages: 899-912

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] An astrocyte-specific enhancer of aquaporin-4 gene functions through a consensus sequence of POU transcription factors in concert with multiple upstream elements2012

    • Author(s)
      Abe Y, Ikeshima-Kataoka H, Goda W, Niikura T., Yasui M
    • Journal Title

      J.Neurochem

      Volume: 120(6) Pages: 899-912

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Quiescence and _YH2AX in Neuroblastoma are Regulated by Ouabain/Na, K-ATPase2012

    • Author(s)
      Hiyoshi H, Abdelhady S, Segerstrom L, Sveinbjornsson B, Nuriya M, Lundgren T.K, Desfrere L, Miyakawa A, Yasui M, Kogne P, Johnsen J.I, Andang M, Uhlen P
    • Journal Title

      British Journal of Cancer

      Volume: (in press)

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Apoptosis in mouse amniotic epithelium is induced by activated macrophages through the TNF receptor type 1/TNF pathway2011

    • Author(s)
      Kobayashi K, Umezawa K., Yasui M.
    • Journal Title

      Biology of Reproduction

      Volume: 84 Pages: 248-54

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Progesterone maintains amniotic tight junctions during midpregnancy in mice2011

    • Author(s)
      Kobayashi K, Miwa H., Yasui M
    • Journal Title

      Mol Cell Endocrinol

      Volume: 337(1-2) Pages: 36-42

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Curcumin and genistein additively potentiate G551D-CFTR2011

    • Author(s)
      Yu YC*, Miki H*, Nakamura Y, Hanyuda A, Matsuzaki Y, Abe Y, Yasui M, Tanaka K, Hwang TC, Bompadre SB., Sohma Y (*co-first authors)
    • Journal Title

      Journal ot Cystic Fibrosis

      Volume: 10(4) Pages: 243-52

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plasma membrane aquaporin AqpZ is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp.PCC 68032011

    • Author(s)
      Akai M, Onai K, Kusano M, Sato M, Redestig H, Toyooka K, Morishita M, Miyake H, Hazama A, Checchetto V, Szabo I, Matsuoka K, Saito K, Yasui M, Ishiura M., Uozumi N
    • Journal Title

      Journal of Biological Chemistry

      Volume: 286(28) Pages: 25224-35

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-Gaussian Fluctuations Resulting from Power-Law Trapping in a Lipid Bilayer2011

    • Author(s)
      Akimoto T, Yamamoto E, Yasuoka K, Hirano Y., Yasui M
    • Journal Title

      Phys.Rev.Lett.

      Volume: 107(17) Pages: 178103-7

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Analysis of Aquaporin-Mediated Diffusional Water Permeability by Coherent Anti-Stokes Raman Scattering Microscopy2011

    • Author(s)
      Ibata K, Takimoto S, Morisaku T, Atsushi Miyawaki., Yasui M
    • Journal Title

      Biophys J

      Volume: 101(9) Pages: 2277-2283

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of water permeability through aquaporin-42010

    • Author(s)
      Yukutake, Y., Yasui, M.
    • Journal Title

      Neuroscience (Epub ahead of print)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Mechanisms How Mercury Inhibits Water Permeation of Aquaporin-1 : Understanding by Molecular Dynamics Simulation2010

    • Author(s)
      Hirano Y, Okimoto N, Yasuoka K, Taiji M, Suematsu M, Yasui M
    • Journal Title

      Biophys.J. (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expression and distribution of tight junction proteins in human amnion during late pregnancy2010

    • Author(s)
      Kobayashi, K., Kadohira, I., Tanaka, M., Yoshimura, Y., Ikeda, K., Yasui, M.
    • Journal Title

      Placenta 31

      Pages: 158-162

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of Kv2.1 phosphorylation in an animal model of anoxia2010

    • Author(s)
      Ito T, Nuriya M, Yasui M.
    • Journal Title

      Neurobiol.Dis. 38

      Pages: 85-91

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Activity-dependent regulation of HCN1 protein in cortical neurons2009

    • Author(s)
      Arimitsu T, Nuriya M, Ikeda K, Takahashi T, Yasui M
    • Journal Title

      Biochem.Biophys.Res.Commun. 387

      Pages: 87-91

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila big brain does not act as a water channel, but mediates cell adhesion2009

    • Author(s)
      Tatsumi K, Tsuji S, Miwa H, Morisaku T, Nuriya M, Orihara M, Kaneko K, Okano H, Yasui M.
    • Journal Title

      FEBS Lett. 583

      Pages: 2077-2082

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] TSHR Mutations as a Cause of Congenital Hypothyroidism in Japan : a Population-Based Genetic Epidemiology Study2009

    • Author(s)
      Narumi S, Muroya K, Abe Y, Yasui M, Asakura Y, Adachi M, Hasegawa T
    • Journal Title

      J.Clin.Endocrinol.Metab. 94

      Pages: 1317-1323

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rapid and reversible inhibition of aquaporin-4 by zinc2009

    • Author(s)
      Yukutake Y, Hirano Y, Suematsu M, Yasui M
    • Journal Title

      Biochem. 48

      Pages: 12059-12061

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic changes in amniotic tight junctions during pregnancy2009

    • Author(s)
      Kobayashi, K., Inai, T., Shibata, Y., Yasui, M
    • Journal Title

      Placenta 30

      Pages: 840-847

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] pH regulated anion permeability of aquaporin-62009

    • Author(s)
      Yasui, M.
    • Journal Title

      Handb.Exp.Pharmacol. 190

      Pages: 299-308

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aquaporin 6 binds calmodulin in a calcium-dependent manner2009

    • Author(s)
      Rabaud, N.E., Song, L., Wang, Y., Agre, P., Yasui, M., Carbrey, J.M.
    • Journal Title

      Biochem.Biophys.Res.Commun. 383

      Pages: 54-57

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Aquaporin-6 is expressed along the rat gastrointestinal tract and upregulated by feeding in the small intestine2009

    • Author(s)
      Laforenza, U., Gastaldi, G., Polimeni, M., Tritto, S., Tosco, M., Ventura, U., Scaffino, M.F., Yasui, M.
    • Journal Title

      BMC Physiol. 9

      Pages: 1-12

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Membrane potential dynamics of axons in cultured hippocampal neurons probed by second harmonic generation imaging2009

    • Author(s)
      Nuriya M, Yasui M.
    • Journal Title

      Journal of Biomedical Optics (in press)

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Water Biology:水分子ナノ動態から高次脳機能に迫る2011

    • Author(s)
      安井 正人
    • Organizer
      第5 回トランスポーター研究会九州部会
    • Place of Presentation
      ホテルJAL シティ 宮崎
    • Year and Date
      2011-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Water Biology:水分子ナノ動態から高次脳機能に迫る2011

    • Author(s)
      安井正人
    • Organizer
      第5回トランスポーター研究会九州部会
    • Place of Presentation
      ホテルJALシティ宮崎(宮崎)(招待講演)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Water Biology:水分子ナノ動態から高次脳機能に迫る2011

    • Author(s)
      安井 正人
    • Organizer
      創薬薬理フォーラム第19 回シンポジウム
    • Year and Date
      2011-09-13
    • Related Report
      2011 Final Research Report
  • [Presentation] Water Biology:水分子ナノ動態から高次脳機能に迫る2011

    • Author(s)
      安井正人
    • Organizer
      創薬薬理フォーラム第19回シンポジウム
    • Place of Presentation
      日本薬学会長井記念館(東京)(招待講演)
    • Year and Date
      2011-09-13
    • Related Report
      2011 Annual Research Report
  • [Presentation] Roles of aquaporins in water dynamics of the cells2011

    • Author(s)
      安井正人
    • Organizer
      International Conference "Water and Nanomedicine"
    • Place of Presentation
      Academy of Sciences and Arts of Republic of Srpska Banja Luka (Bosnia and Herzegovina)(招待講演)
    • Year and Date
      2011-08-30
    • Related Report
      2011 Annual Research Report
  • [Presentation] Roles of aquaporins in water dynamics of the cells2011

    • Author(s)
      安井 正人
    • Organizer
      International Conference "Water and Nanomedicine"
    • Place of Presentation
      Academy of Sciences and Arts of Republic of Srpska Banja Luka
    • Year and Date
      2011-08-16
    • Related Report
      2011 Final Research Report
  • [Presentation] AQP と脳水腫2011

    • Author(s)
      安井 正人
    • Organizer
      北京大学医学部特別講演会
    • Place of Presentation
      北京大学医学部
    • Year and Date
      2011-08-16
    • Related Report
      2011 Final Research Report
  • [Presentation] AQPと脳水腫2011

    • Author(s)
      安井正人
    • Organizer
      北京大学医学部特別講演会
    • Place of Presentation
      北京大学医学部(北京、中国)(招待講演)
    • Year and Date
      2011-08-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] アクアポリンとドライシンドローム2011

    • Author(s)
      安井 正人
    • Organizer
      日本ドライシンドローム学会設立記念講演会
    • Year and Date
      2011-03-04
    • Related Report
      2011 Final Research Report
  • [Presentation] Roles of AQP4 in brain disorders, THE FIRST WORLD CONGRESS ON WATER CHANNEL PROTEINS (AQUAPORINS AND RELATIVES)2011

    • Author(s)
      安井 正人
    • Organizer
      CELEBRATING THE 25TH ANNIVERSARY OF THE DISCOVERY OF THE FIRST WATER CHANNEL PROTEIN(LATER CALLED AQUAPORIN 1)
    • Place of Presentation
      CLUJ-NAPOCA, ROMANIA
    • Related Report
      2011 Final Research Report
  • [Presentation] Perinatal dynamism of water balance and role of aquaporin2011

    • Author(s)
      安井 正人
    • Organizer
      第11 回アジア小児腎臓学会学術集会, 第46 回日本小児腎臓病学会学術集会
    • Place of Presentation
      福岡国際会議場
    • Related Report
      2011 Final Research Report
  • [Presentation] Perinatal dynamism of water balance and role of aquaporin2011

    • Author(s)
      安井正人
    • Organizer
      第11回アジア小児腎臓学会学術集会第46回日本小児腎臓病学会学術集会
    • Place of Presentation
      福岡国際会議場(福岡)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Roles of AQP4 in brain disorders2011

    • Author(s)
      安井正人
    • Organizer
      THE FIRST WORLD CONGRESS ON WATER CHANNEL PROTEINS (AQUAPORINS AND RELATIVES) CELEBRATING THE 25TH ANNIVERSARY OF THE DISCOVEEY OF THE FIRST WATER CHANNEL PEOTEIN (LATER CALLED AQUAPORIN 1)
    • Place of Presentation
      CLUJ-NAPOCA, ROMANIA(招待講演)
    • Related Report
      2011 Annual Research Report
  • [Presentation] Integrated physiology in epithelial transport using non-linear optics and computer modeling2010

    • Author(s)
      安井正人
    • Organizer
      第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] Aquaporin and Water Seen by Two Photon Microscopy2009

    • Author(s)
      安井 正人
    • Organizer
      WINPTech2009 Workshop on Information, Nano and Photonics Technology 2009
    • Place of Presentation
      KOBE University Centennial Hall and Takikawa Memorial Hall, Kobe University
    • Year and Date
      2009-12-02
    • Related Report
      2011 Final Research Report
  • [Presentation] Aquaporin and Water Seen by Two Photon Microscopy2009

    • Author(s)
      安井正人
    • Organizer
      WINPTech2009 Workshop on Information, Nano and Photonics Technology 2009
    • Place of Presentation
      KOBE University Centennial Hall and Takikawa Memorial Hall, Kobe University, Kobe, Japan
    • Year and Date
      2009-12-02
    • Related Report
      2009 Annual Research Report
  • [Presentation] アクアポリン-4 の調節機構2009

    • Author(s)
      安井 正人
    • Organizer
      第39 回慶應ニューロサイエンス研究会, アクアポリン-4 と神経疾患-新たな病態メカニズムの展開と治療への戦略
    • Place of Presentation
      慶應義塾大学医学部 北里講堂
    • Year and Date
      2009-10-31
    • Related Report
      2011 Final Research Report
  • [Presentation] アクアポリン-4の調節機構2009

    • Author(s)
      安井正人
    • Organizer
      第39回慶應ニューロサイエンス研究会『アクアポリン-4と神経疾患-新たな病態メカニズムの展開と治療への戦略』
    • Place of Presentation
      慶應義塾大学医学部 北里講堂
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] Clinical relevance of water channel aquaporin2009

    • Author(s)
      安井 正人
    • Organizer
      The First International Meeting on Japan - China - Korea Traditional Medicine
    • Place of Presentation
      昭和薬科大学記念講堂
    • Year and Date
      2009-10-25
    • Related Report
      2011 Final Research Report
  • [Presentation] Clinical relevance of water channel, aquaporin2009

    • Author(s)
      安井正人
    • Organizer
      The First International Meeting on Japan-China-Korea Traditional Medicine
    • Place of Presentation
      昭和薬科大学記念講堂(講義棟2F)
    • Year and Date
      2009-10-25
    • Related Report
      2009 Annual Research Report
  • [Presentation] Aquaporins in brain disorders2009

    • Author(s)
      安井 正人
    • Organizer
      北京大学医学部
    • Place of Presentation
      北京大学
    • Year and Date
      2009-09-16
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Presentation] Regulation, structure, and function of brain aquaporins2009

    • Author(s)
      安井 正人
    • Organizer
      The 22nd Biennial Joint Meeting of the International Society for Neurochemistry (ISN) and the Asian Pacific Society for Neurochemistry(APSN)
    • Place of Presentation
      BEXCO, Busan, Korea,
    • Year and Date
      2009-08-24
    • Related Report
      2011 Final Research Report
  • [Presentation] Regulation, structure, and function of brain aquaporins2009

    • Author(s)
      安井正人
    • Organizer
      The 22nd Biennial Joint Meeting of the International Society for Neurochemistry(ISN)and the Asian Pacific Society for Neurochemistry(APSN), Busan, Korea
    • Place of Presentation
      BEXCO, Busan, Korea
    • Year and Date
      2009-08-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] 水代謝の生理と病態に関する最新の話題ーアクアポリンの構造機能相関と分子標的創薬2009

    • Author(s)
      安井 正人
    • Organizer
      第44回日本小児腎臓病学会学術集会
    • Place of Presentation
      一橋記念講堂
    • Year and Date
      2009-06-26
    • Related Report
      2011 Final Research Report
  • [Presentation] 水代謝の生理と病態に関する最新の話題-アクアポリンの構造機能相関と分子標的創薬2009

    • Author(s)
      安井正人
    • Organizer
      第44回日本小児腎臓病学会学術集会
    • Place of Presentation
      一橋記念講堂
    • Year and Date
      2009-06-26
    • Related Report
      2009 Annual Research Report
  • [Presentation] 中枢神経におけるアクアポリン-4(AQP4)の役割2009

    • Author(s)
      安井 正人
    • Organizer
      第50回日本神経学会総会
    • Place of Presentation
      仙台国際センター
    • Year and Date
      2009-05-22
    • Related Report
      2011 Final Research Report 2009 Annual Research Report
  • [Remarks]

    • URL

      http://web.sc.itc.keio.ac.jp/pharm/

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://web.sc.itc.keio.ac.jp/pharm/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://web.sc.itc.keio.ac.jp/pharm/index-jp.html

    • Related Report
      2010 Annual Research Report
  • [Remarks] 薬理学教室HP

    • URL

      http://web.sc.itc.keio.ac.jp/pharm/index-jp.html

    • Related Report
      2009 Annual Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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